• The Plant Pathology Journal
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• 제38권6호
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• pp.685-691
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• 2022

Plants produce chemicals of immense diversity that provide great opportunities for development of new antifungal compounds. In search for environment-friendly alternatives to the fungicide of current use, we screened plant extracts obtained from more than eight hundred plant materials collected in Korea for their antifungal activity against the model plant pathogenic fungus, Magnaporthe oryzae. This initial screening identified antifungal activities from the eleven plant extract samples, among which nine showed reproducibility in the follow-up screening. These nine samples were able to suppress not only M. oryzae but also other fungal pathogens. Interestingly, the plant extracts obtained from Actinostemma lobatum comprised five out of eight samples, and were the most effective in their antifungal activity. We found that butanol fraction of the A. lobatum extract is the most potent. Identification and characterization of antifungal substances in the A. lobatum extracts would provide the promising lead compounds for new fungicide.

• 한국미생물·생명공학회지
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• 제37권3호
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• pp.273-279
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• 2009

형방지황탕은 한의학에서 중요한 약처방으로 인간의 기력 회복에 약효가 탁월한 것으로 알려져 있다. 본 연구에서는 형방지황탕으로부터 항생 물질(HTI)을 분리하여 항진균활성 및 그 작용기작을 검토하였다. 그 결과 각종 병원성 진균에 대해 항진균 활성을 나타내었으며, 인간 적혈구에 대해서는 세포독성이 나타나지 않은 것으로 확인되었다. 또한 캔디다 알비칸스를 대상으로 스트레스 방어기작과 세포 이형성 유도에 HTI이 미치는 영향을 검토한 결과, 트리 할로즈의 증가와 이형성 유도에 영향을 미치는 것으로 나타나 이러한 현상이 항진균활성에 상관관계가 있음을 확인하였으며, 또한 진균의 세포주기에 미치는 영향도 항진균 활성의 요인이 됨을 밝혔다.

• Journal of Microbiology and Biotechnology
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• 제17권7호
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• pp.1217-1220
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• 2007

The saprophytic fungus Ulocladium atrum Preuss is a promising biological control agent for Botrytis cinerea in greenhouse- and field-grown crops. However, despite its known potent antifungal activity, no antifungal substance has yet been reported. In an effort to characterize the antifungal substance from U. atrum, we isolated an antibiotic peptide. Based on extensive spectroscopic analyses, its structure was established as a cyclopeptolide with a high portion of N-methylated amino acids, and its $^1H$ and $^{13}C$ chemical shifts were completely assigned based on extensive 1D and 2D NMR experiments. Compound 1 exhibited potent antifungal activity against the plant pathogenic fungus Botrytis cinerea and moderate activity against Alternaria alternate and Magnaporthe grisea.

• 생약학회지
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• 제34권4호통권135호
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• pp.303-307
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• 2003

For the purpose of searching for the new antifungal agent from the plant origin, we have examined twenty seven species of medicinal plants for the antifungal activity against Candida albicans and Pityrosporum ovale. The whole MeOH extract of each plant material and the n-hexane and EtOAc fractions were evaluated individually for the antifungal activity. Among the test materials, the fractions from the root bark of Zanthoxylum schinifolium, Zanthoxylum piperitum and Citrus unshiu showed significant antifungal activities in a dose dependent manner against Pityrosporum ovale and Candida albicans, respectively.

• Mycobiology
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• 제28권4호
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• pp.190-192
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• 2000

Antifungal bacteria for biological control of plant diseases or production of novel antibiotics to plant pathogens were isolated in 1997 from various soils of Ansung, Chunan, Koyang, and Paju in Korea. Sixty-four bacterial strains pre-screened from approximately 1,400 strains were tested on V-8 juice agar against eight plant pathogenic fungi using in vitro bioassay technique for inhibition of mycelial growth. Test pathogens were Alternaria mali, Colletotrichum gloeosporioides, C. orbiculare, Fusarium oxysporum f. sp. cucumerinum, F. oxysporum f. sp. lycopersici, Magnaporthe grisea, Phytophthora capsici, and Rhizoctonia solani. A wide range of antifungal activity of bacterial strains was found against the pathogenic fungi, and strain RC-B77 showed the best antifungal activity. Correlation analysis between inhibition of each fungus and mean inhibition of all eight fungi by 64 bacterial strains revealed that C. gloeosporioides would be best appropriate for detecting bacterial strains producing antibiotics with potential as biocontrol agents for plant pathogens.

• 보존과학연구
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• 통권30호
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• pp.157-170
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• 2009

We have evaluated the antifungal activities of oak vinegar to develop a natural biocide for organic cultural heritage. Fungi used in this study were screened from the cultural heritages, Kyujanggak and JanggyeongPanjeon and tested on organic substrates-degrading ability. In the results, 7 species of fungi have produced the extracellular enzymes to degrade CMC, xylan, lignin. Thus, we have used these seven species fungi to investigate the antifungal activity of oak vinegar in this study. In the result, the antifungal activity of oak vinegar indicated positive potencial. Especially, methylene chloride and ethylacetate fractions of the oak vinegar had high activities at the concentration of 5.0mg/disc. In these fractions, many different kinds of compounds such as phenolic and furfural, etc. were analyzed by GC-MS. The experiments indicated that the development of a biocide using natural extracts can have a potential to conserve of organic cultural heritages.

• 약학회지
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• 제45권5호
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• pp.431-436
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• 2001

Antifungal activities of trans-cinnamaldehyde (CA) derivatives including commercial CA derivatives as well as synthesized CA derivatives against various human pathogenic fungi were investigated. Among the derivatives tested, -chlorocinnamaldehyde, $\alpha$-bromocinnamaldehyde and 7-phenyl-2,4,6-heptatrienal were more potent than CA in antifungal activity, $\alpha$-Bromocinnamaldehyde was the most effective in inhibiting the growth of representative fungi of dermatomycosis with minimum inhibitory cocentration(MIC) of 0.61~9.76$\mu\textrm{g}$/ml . In the structure-activity relationship, introduction of the chlorine and bromine group into the C-2 of CA resulted in the decrease of MIC. Derivative with more double bonds exhibited the increase of antifungal activity against various pathogenic fungi.

• Archives of Pharmacal Research
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• 제28권7호
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• pp.750-755
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• 2005

A series of 2-arylamino-5-hydroxy-naphthalene-1,4-diones, 3-arylamino-5-methoxy-naphthalene-1,4-diones, and 2-arylamino-3chloro-5-hydroxy-naphthalene-1,4-diones were synthesized and tested for in vitro antifungal activity against the species Candida and Aspergillus niger. Among those tested, 3-arylamino-5-methoxy-naphthalene-1,4-diones exhibited potent antifungal activity. In general, the 3-arylamino-5-methoxy-naphthalene-1,4-diones showed more potent antifungal activity than the 2-arylamino-5-hydroxy-naphthalene-1,4-diones and the 2-arylamino-3-chloro-5-hydroxy-naphthalene-1,4-diones.

• 한국약용작물학회지
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• 제17권6호
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• pp.464-469
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• 2009

This study was conducted to find out environment-friendly disease control method to Alternaria blight caused by Alternaria panax of ginseng. For this study, 150 methanol extracts from medicinal plants were evaluated and the extract of Impatiens balsamina showed most strong antifungal activity against A. panax. The methanol extract of I. balsamina showed also stable antifungal activity against other ginseng pathogens such as Botrytis cinerea and Fusarium sp., when treated by heat or pH. In vivo, Alternaria blight incidence rate was low of 13% with the treatment of I. balsamina methanol extract compared to 35% of the non-treatment. The antifungal compound of I. balsamina was purified and identified by using a silica gel column chromatography, TLC and ESI-LC/MS/MS analysis. The compound which showed strong antifungal activity was identified as 2-methoxy-1,4-naphtoquinone ($C_{11}H_8O_3$).

• 미생물학회지
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• 제38권3호
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• pp.162-167
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• 2002

항진균 활성 관련 단백질을 찾기 위해 미역류로부터 Streptomyces sp. SAR01을 분리하고, FAME (fatty acid methyl ester)분석으로 동정하였다. 감마선(sup $({60}^Co)$)조사를 이용하여 항진균 활성 결핍 돌연변이체(SAR535)를 유도한 후, 이차원전기영동으로 단백질을 분석한 결과 SAR01에만 존재하는 6종의 단백질을 확인할 수 있었다. 이중 10 kDa chaperonin cpn10 (GroES)과 96%의 상동성을 가진 10 kDa의 단백질을 클로닝하였으며, E. coli Ml5에서 과대 발현됨을 확인하였다. 또한 SAR535에 형질전환시킨 결과 SAR01과 유사한 항진균 활성이 나타났다. 이것으로 볼 때 groES는 Streptomyces sp. SAR01의 항진균 활성에 관련된 것으로 사료된다.