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groES Expression Related to Antifungal Activity of Streptomyces sp. SAR01  

이영근 (한국원자력연구소 R. 방사선응용연구팀)
김재성 (한국원자력연구소 R. 방사선응용연구팀)
조규성 (한국원자력연구소 R. 방사선응용연구팀)
장병일 (한국원자력연구소 R. 방사선응용연구팀)
추철형 (한국원자력연구소 R. 방사선응용연구팀)
Publication Information
Korean Journal of Microbiology / v.38, no.3, 2002 , pp. 162-167 More about this Journal
Abstract
To analyse proteins and gene related to antifungal activity, SAR01 strain was isolated from a brown seaweed and identified as Streptomyces sp. by FAME(fatty acid methyl ester) analysis. Antifungal activity deficient mutant(SAR535) of Streptomyces sp. SAR01 was induced by gamma radiation$({60}^Co)$. It was found that 6 specific protein spots appeared only in SAR01 by 2-D electrophoresis analysis. Among them, a protein of 10 kDa had homology of 96% with 10 kD chaperonin cpn 10 (GroES) by Basic Local Alignment Search Tool(BLAST, NCBI) analysis. SAR535 transformants into which groES was transferred by electroporation revealed antifungal activity newly similar with SAR01 It suggested that groES be supposed to be related to the antifungal activity of Streptomyces sp. SAR01.
Keywords
antifungal protein; 2-DE; gamma radiation; groES; mutant; Streptomyces;
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