• Archives of Plastic Surgery
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• v.37 no.2
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• pp.115-121
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• 2010

Purpose: Many topical agents had been used for contaminated wound treatment. Although antimicrobial ointments were widely used as topical agents, their comparative antibacterial and wound healing effects were largely unreported. The purpose of this study was to compare antibacterial effects and wound healing effects of common topical ointments on infected full thickness skin defect in the mouse. Methods: One full thickness skin defects in the mice (n=60) were developed on the back and left open for twenty-four hours. Sixty mice were divided into four groups: group B (dressing with Bactroban$^{(R)}$, n=15), group I (dressing with Iodosorb$^{(R)}$, n=15), group T (dressing with Terramycin$^{(R)}$, n=15), group G (control group, dressing with dry gauze, n=15). The size of wound defects and the grades of wound healing were evaluated at 4, 7, 10 days, and antibacterial effect was evaluated with restricted zone in Mueller Hinton agar by disk diffusion method. After the wound was left open for twenty-four hours, many Staphylococcus aureus was cultured. The wound defect size was decreased in order of Bactroban$^{(R)}$ (B), Iodosorb$^{(R)}$ (I), Terramycin$^{(R)}$ and gauze dressing group in all days, but difference among experimental groups was not statistically significant. The grade score of wound healing was increased in order of Bactroban$^{(R)}$, Iodosorb$^{(R)}$, Terramycin$^{(R)}$ and gauze dressing group, and the difference was statistically significant. Antibacterial effect for was increased in order of Bactroban$^{(R)}$, Iodosorb$^{(R)}$, Terramycin$^{(R)}$ and gauze dressing group, and the difference was statistically significant. Conclusion: Topical antimicrobial ointments application was effective against wound infections by S. aureus. Bactroban$^{(R)}$ may be an optimal topical treatment for infected wounds according to this study. However, further study is necessary to evaluate the clinical efficacy of antimicrobial ointments and to search for the mechanisms that explain their effects.

• The Journal of the Korea Contents Association
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• v.17 no.10
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• pp.300-306
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• 2017

The purpose of this study was to investigate the antibacterial effect of functional multiwall carbon nanotube (MWCNT) on the conventional glass ionomer cement(GIC). The MWCNT was incorporated into a commercial powder at 0.125, 0.25, 0.5 and 1.0 wt%. Specimens for vickers hardness(VHN) disks(Ø$10mm{\times}2mm$) each were prepared. Indention were made using a load of 200 N and 20 s dwell on four specimens for each distilled water storage time 1d, 7d, and 14days. The antibacterial effects using agar diffusion test with S. mutans and S. aureus. The diameters of the inhibition zones produced around the materials were measured. The results revealed that all the vickers hardness values were increase significantly with incubation time(p<0.05). Regarding the antibacterial effect for S. mutans, all the tested groups showed a slightly higher value not significantly with the control group(p<0.05). However S. aureus statistical analysis indicated a significant difference for antibacterial agents between control and MWCNT containing 0.25 wt%(p<0.05). These results of this study provide that the conventional GIC with containing MWCNT show good antibacterial effect against and favorable mechanical properties. Further this study on the efficient functionalization of multiwall carbon nanotube will be needed.

• YAKHAK HOEJI
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• v.41 no.2
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• pp.225-232
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• 1997

The in vitro antibacterial activity of DWP20367 (1-Cyclopropyl-6-fluoro-8-chloro-7-(2,7-diazabicyclo[3,3,0]oct-4-ene-7-yl)-1,4-dihydro-4-oxoquinoline-3-carboxylic acid), a new broad-spectrum fluoroquinolone, was compared with those of ciprofloxacin (CPFX), sparfloxacin (SPFX) and ofloxacin (OFLX). DWP20367 was showed antibacterial activity much higher than CPFX, SPFX and OFLK against gram-positive bacteria, while it was slightly more superior to quinolones against gram-negative bacteria. DWP20367 was particularly effective against MRSA, and its $MlC_{90}$ against clinical isolates of methicillin-susceptible S. aureus, low methicillin-resistant S. aureus and high methicillin-resistant S. aureus were 0.098, 0.781 and 1.563 micro g/ml, respectively. Against S. pneumoniae, MIC90 of DWP20367 was 2- to 8-fold higher than those of CPFX. With a view of MIC90, DWP20367 showed slightly more potent activity against P. aeruginosa and E. coli isolates than the control quinolones. DWP20367 activity was not affected by inoculum size and medium pH. But addition of $Mg^{2+}, \;Ca^{2+} $Mg2+, Ca2+ or horse serum (25%) decreased its antibacterial activity. DWP20367 was showed rapidly bactericidal activity within 2 to 4 h, and regrowth was not observed even after 24 h incubation at concentrations near the 4-fold of MIC.

• Journal of Food Hygiene and Safety
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• v.25 no.1
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• pp.6-9
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• 2010

The present study was undertaken to estimate the antibacterial effect of a combination of C. rhizoma, L. Flos, and P japonica (1:1:1) extracts (CLP1000) and a combination of the herbal extract mixture and dioctahedral smectite (CLPS1000) against murine salmonellosis. At the concentration of CLP1000 and CLPS1000 0.5 mg/ml, the antibacterial effect was not showed on Salmonella typhimurium (S. typhimurium). On the other hand, the antibacterial effect against S. typhimurium was observed at the concentration of CLP1000 and CLPS1000 1.0 mg/ml. Oral administration of Smectite, CLPl000, and CLPS1000 at the dose of 10 mg/ml showed a therapeutic effect for S. typhimurium infected BALB/c mice. The mortality of Smectite, CLP1000 and CLPS1000-treated mice was 90%, 90%, and 70% at 12 days, respectively, while that of untreated mice was 100% at 9 days after a lethal dose of S. typhimurium infection. The results of our study strongly indicate that CLPS1000 has potential as an effective of salmonellosis.

• Journal of Korean Medicine Rehabilitation
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• v.28 no.2
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• pp.21-35
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• 2018

Objectives The objective of this study is to determine the antimicrobial effect of Daehwanggyeonu-san(Dahwangqianniu-san,DGE) and synergistic effects with antibiotics oxacillin, ampicillin, and gentamicin against Methicillin-Resistant Staphylococcus aureus(MRSA). Methods The antibacterial activity of DGE extract was evaluated againest MRSA strains by using the Disc diffusion method, broth microdilution method(minimal inhibitory concentration; MIC), checkerboard dilution test. The checkerboard dilution test was used to examined synergetic effect of oxacillin, ampicillin, gentamicin, ciprofloxacin with DGE extract. Results DGE showed antimicrobial activity against MRSA with an MIC value of $125{\sim}250{\mu}g/mL$. In the checkerboard test, the interation of DGE with all tested antibiotics produced almost synergy or partial synergy against MRSA. Conclusions This study shows that DGE reduced the MICs of several antibiotics tested, and a remarkable antibacterial effect of DGE, with membrane permeability enhancers and ATP synthase inhibitors. This study can be a valuable source for the development of a new drug with low MRSA resistance.

• Journal of the Korean Society of Food Culture
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• v.16 no.3
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• pp.243-249
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• 2001

Antibacterial activities in each part of watercress(Oenanthe javanica D.C.) grown under different culture conditions were measured to determine the possibility to use watercress as a resource to develop the antibacterial substance. The leaves of watercress were extracted with methanol and the methanol extract was further fractionated with various organic solvents. Antibacterial activities against Shigella dysenteriae ATCC 9361 in all fractions were determined according to the agar diffusion method using paper disc. Methanol extract of watercress leaves was more effectively inhibited the growth of the tested bacteria than the extracts of roots or stems at the concentration of 0.5 g eq./disc, and the extract of watercress from Hwasoon was the most effective one as compared to others. Phenolic and neutral fractions fractionated from methanol extract of watercress had a considerable inhibiting activity on the growth of the bacteria, but acidic and basic fractions did not show any inhibitory effect. Minimum inhibitory concentrations of phenolic and neutral fractions against Shigella dysenteriae ATCC 9361 were $400\;{\mu}g/disc$ and $550\;{\mu}g/disc$, respectively.

• The Korea Journal of Herbology
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• v.25 no.3
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• pp.111-116
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• 2010

Objectives : This experimental study was performed to investigate the antibacterial effect of Caesalpinia sappan and Coptis chinensis extract against B. cereus and V. parahaemolyticus. Methods : Methanol extracts of C. sappan and C. chinensis was tested against B. cereus and V. parahaemolyticus by paper disc method. Results : The growth of B. cereus and V. parahaemolyticus was inhibited by C. sappan and C. chinensis extract among 6 kinds of medicinal plant extracts. The extract of C. sappan and C. chinensis extract inhibited the growth of V. parahaemolyticus and B. cereus, respectively. The growth of B. cereus and V. parahaemolyticus had a tendency to increase depend on the concentration of the extract. EtOEt and EtOAc fractions and EtOEt and BuOH fractions of the C. sappan extract had a high antibacterial activity against B. cereus and V. parahaemolyticus, respectively. And, BuOH and $H_2O$ fractions of the C. chinensis extract showed antibacterial activity against B. cereus highly. Conclusions : C. sappan and C. chinensis extract efficiently inhibited the growth of B. cereus and V. parahaemolyticus.

• Microbiology and Biotechnology Letters
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• v.20 no.1
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• pp.1-5
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• 1992

Antibacterial activity of the water-soluble portion of Ulmus pzcmila L. extract against 10 bacterial species was studied by both cylinder plate dilution method and broth dilution test tube method. Inhibitory effect of the extract on the bacteria was also investigated by plotting bacterial survival at various concentration of the extract. The crude extract exhibited antibacterial activity against all of the tested bacterial species with exception of K pneurnoniae. The fractions of the extract prepared by CM Sephadex-C 50 ion exchange chromatography were also subjected to test the antibacterial activity, and the activity was studied after autoclaving for 20 minutes.

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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• 2006.06b
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• pp.417-421
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• 2006

In this study, colloidal Ag solution was spouted on the surface of the inorganic pigment using the hybridizer system and the spray nozzle. Then, the surface of the inorganic pigment was modified by titanium dioxide in order to possess antibacterial ability. Nano-sized colloidal Ag was made by using a seed sol method in this study. It was confirmed that the size of particle per unit weight becomes enlarged, as the addition of $AgNO_{3}$ increased, and as the time of reaction increased, in the manufacturing process of nano-sized colloidal Ag. The antibacterial measurement of the inorganic pigment showed that the growth of fungus was reduced as the reaction time increased. It was measured that the antibacterial activity was excellent at fixed time frame, after the antibacterial ability appeared in $5{\sim}7$ hours of the antibacterial inoculation experiment. The experiment of titanium dioxide's Photocatalyst effect showed $60{\sim}70%$ efficiency in about 80 minute reaction time of the dissolution results regarding measurements of benzene. It was shown that more than 90% of the dissolution efficiency was achieved in the reaction time of about 30 minute.

• Journal of Life Science
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• v.11 no.6
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• pp.554-560
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• 2001

For development of functional food, antibacterial effect of Poncirus trifoliata juice was examined. Strong antibacterial activities of Poncirus trifoliata juice were observed aginst Gram positive and negative pathogenic bacteria such as Baillus cereus, Bacillus subtilis, Corynebacterium zerosis, Listeria monocytogenes, Micrococcus luteus, Rhodococcus equi, Klebsiella pneumoniae, Pseudomonas aeruginosa, Vibrio alginolyticus, Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vnlnificus and Yersinia enterocolitica. The minimum inhibitory concentration(MIC) of Poncirus trifoliata juice against Bacillus cereus. Listeria monocytogenes, Micrococcus luteus, Rhodococcus equi, Staphylococcus epidermidis, Citrobacter freundil and Pseudomonas aeruginosa was 2.5% and the MIC against Vibrio cholerae, Vibrio parahemolyticus, Vibrio vulnificus and Yersinia enterocolitica was 1.25%. Also, antibacterial activities of Poncirus trifoliata juice treated for 15 min at 121$^{\circ}C$ were confirmed to be stable.