• 한국응용과학기술학회지
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• 제34권4호
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• pp.924-937
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• 2017

2016년 4월부터 5월까지 20대부터 60대까지의 국내 성인 여성들을 대상으로 설문지 366부를 본 연구에 사용하였다. 천연 소재를 활용하여 항산화 맥주의 활용 가능성을 확인하고자 항산화 활성을 측정하였고, 항산화 수제 맥주에 대한 선호도 조사를 실시하였다. 연구 결과, 연구 대상자는 음주 인식에 대해 긍정적으로 확인되었으며, 국내 성인 여성들은 맥주에 대한 선호도가 높으며, 체력, 기분, 상황, 장소, 동행자 유형에 따라 음주 형태는 달라지는 것을 확인 할 수 있었다. 또한 주정으로 추출된 천연 소재에서 높은 항산화 활성 효과를 확인하였고, 수제 맥주 선호도 조사에서는 아로니아 열매를 첨가한 군이 전체적으로 높은 평가를 받은 것으로 확인되었다. 이와 같은 결과를 통해 국내 성인 여성 음주 및 항산화 맥주 개발에 대한 기초 자료가 될 것으로 사료되어 진다.

• Journal of Nutrition and Health
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• 제46권4호
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• pp.315-323
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• 2013

본 연구에서 청미래덩굴 잎과 뿌리의 물추출물을 이용하여 총 폴리페놀과 플라보노이드의 함량을 측정한 결과 잎 추출물에서 더 많은 함량이 확인되었으며, DPPH 및 ABTS radical 소거능, 환원력 측정을 통한 항산화 활성을 비교한 결과도 폴리페놀과 플라보노이드함량 측정 결과와 마찬가지로 잎에서 높은 활성을 명확하게 확인할 수 있었다. 그리고 청미래덩굴 추출물의 항당뇨 효능에 대한 최초의 시도로서 ${\alpha}$-glucosidase 활성억제능을 측정한 결과 뿌리추출물에서는 효소의 억제력을 확인할 수 없었지만 잎 추출물에서는 농도 및 반응시간의 증가에 따라 억제력이 증가하여 물 추출물 상태에서 1 mg/mL 기준으로 acarbose의 ${\alpha}$-glucosidase 활성 억제능과 비교하여 71.8%의 활성을 관찰할 수 있었다. 또한 당뇨와 염증의 관련성이 대두되는 최근의 연구 상황에서 청미래덩굴 잎 추출물의 IL-$1{\beta}$, IL-6, iNOS, COX-2의 mRNA 발현 억제작용을 확인함으로써 염증반응의 개선 가능성도 제시할 수 있었다. 결과적으로 청미래덩굴 잎 추출물의 항산화능, ${\alpha}$-glucosidase 활성억제능 및 항염증효능을 확인함으로써 neutraceuticals 소재로서의 개발가능성을 증명하였고 추가 연구의 필요성을 제시하였다.

• 대한본초학회지
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• 제25권1호
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• pp.83-91
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• 2010

Objectives : In this study, we compared the anti-oxidant and anti-inflammatory activities of Curcumae longae Radix (CLRa) and Curcumae longae Rhizoma (CLRh). Methods : We performed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) cation scavenging assays, and determined total polyphenolic content to examine the anti-oxidant effects of CLRa and CLRh. We also evaluated the anti-oxidant effects of CLRa and CLRh against hydrogen peroxide ($H_2O_2$)-induced toxicity in PC12 cells using thiazolyl blue tetrazolium bromide (MTT) and reactive oxygen species (ROS) assays. Next, to compare the anti-inflammatory effects of CLRa and CLRh against lipopolysaccharide (LPS)-induced inflammation in microglia BV2 cells, we measured nitric oxide (NO) assay and inducible nitrite synthase (iNOS) using Western blotting analysis. Results : CLRa showed higher activity in DPPH and ABTS assays and lower total polyphenolic contents compared with CLRh. In PC12 cells, CLRa and CLRh showed no difference in H2O2-induced cell toxicity and ROS overproduction. In BV2 cells, CLRa showed higher effect than CLRh in NO and iNOS production induced by LPS. Conclusions : These results demonstrate that CLRa has higher radical scavenging activities and anti-inflammatory effect in BV2 cells comparing CLRh. However, CLRa and CLRh have no effect and no difference in $H_2O_2$-induced toxicity.

• 대한의생명과학회지
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• 제24권3호
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• pp.263-269
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• 2018

This study aims to investigate the biological activities related to hair loss of short anti-oxidant peptides (DK peptides) $5{\alpha}$-reductase inhibition and anti-oxidation. The series of DK peptides were generated amphipathic helical properties using leucines, lysines and tryptophan residues. Cell viability and free radical scavenging activities were performed using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. The DK peptides were investigated for inhibitory activity against $5{\alpha}$-reductase. Antioxidant activities were determined by means of, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assays. All peptides could inhibit $5{\alpha}$-reductase in lipopolysaccharide-stimulated macrophage. In conclusion, DK peptides was suggested as the most attractive ingredients for improving hair loss, because of the high inhibitory against $5{\alpha}$-reductase inhibition.

• Biomolecules & Therapeutics
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• 제11권2호
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• pp.139-144
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• 2003

Ganoderma lucidum is commonly known as medically potent mushroom, which has been widely used in China and other oriental countries for the treatment of various diseases, including cancer. In this report, we investigated the anti-oxidant and protective effect of Ganodema lucidum extract (GLE) against the DNA damage induced by free radical and U.V. In the assay of cell growth inhibition, the inhibitory cell growth rate induced by hydroxyl radical was dose-dependently decreased by GLE. This results support that GLE has a detoxifying activity against cytotoxicity of hydroxyl radical in E. coli cell. GLE also protected ColE1 plasmid DNA damage in the concentration of 200$\mu\textrm{g}$ per reaction on the DNA fragmentation assay. The nuclear tailing by hydrogen peroxide in single cell gel electrophoresis(SCGE) was decreased by GLE in the concentration of 50$\mu\textrm{g}$/ml. These data indicate that Ganoderma lucidum has an anti-oxidative activity to hydrogen peroxide. The mutation rate after irradiation of U.V. was reduced by 50$\mu\textrm{g}$/ml GLE and total number of Rif (Rifampicin) resistant mutants was decreased in a concentration dependent manner when added the GLE exogenously in a culture media. According to the results, it is likely that GLE has not only an anti-oxidative activity to hydroxyl radical but also an anti-mutagenic activity to U.V. mutagenesis.

• International Journal of Advanced Culture Technology
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• 제7권4호
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• pp.118-124
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• 2019

There is a strong connection between the diet rich in antioxidants and the decreased incidence of cardiovascular and cancerous diseases. Diets that are rich in anti-oxidants particularly include fruits and vegetables containing the high amounts of vitamin A-E, carotenoids, and minerals. Different processing conditions applied for vegetables and plants results in the alteration of the nutrients present in them. Therefore the rationale of our study was to compare the antioxidant effects of different processed vegetables and plants and to see that which one of them showed best anti-oxidant activity. For this purpose, we have used acetaminophen induced oxidative stress model in mice to check the effects of processed apple, pear, carrot, cabbage, broccoli and radish. Our results have shown that the administration of these samples effectively decreased the expression of parameters related with oxidative stress like ALT, AST, catalase, superoxide dismutase, GPx and 8-OHdG. Moreover they also significantly protected the mice livers from APAP induced damage as shown by histological changes. Therefore our results have demonstrated the effects of processed fruits and vegetables in mice model of oxidative stress.

• 한국융합학회논문지
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• 제9권8호
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• pp.225-233
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• 2018

천연물 유래 난치성 피부질환 기능성 소재를 탐색하기 위해 느릅나무 가지를 초임계 유체 기술을 활용하여 추출을 수행하였고, 확보된 느릅나무 초임계 추출물을 수종의 피부상재균에 대하여 항균력 실험을 실시하였고, 모든 균종에 대해서 항균력이 확인이 되었으며, ABTS 라디컬 소거능과 NO 소거능 실험을 통해서 느릅나무 초임계 추출물의 항산화 활성과 항염증 활성의 우수한 활성이 확인이 되어서 산화적 스트레스 질환과 염증성 피부질환에 대해서 예방 및 치료 보완 기능성 소재로 개발이 가능성이 높다는 것을 알 수 있었다. 결론적으로 느릅나무 초임계 추출물은 피부상재균에 대한 항균활성, 라디컬 소거능에 의한 항산화 활성, 과 생성된 NO 소거능에 의한 항염증활성이 각각 확인이 되었다. 이 상의 결과로 느릅나무 초임계 추출물은 항균효능, 항산화효능, 항염증 효능을 갖는 기능성 원료로서의 가능성을 확인할 수 있었고, 향후 만성 염증성 피부면역 질환 개선 및 치료 보조제 관련 의약품 또는 기능성 향장품 개발을 위한 기초 연구 자료가 될 것으로 사료된다.

• BMB Reports
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• 제36권4호
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• pp.337-343
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• 2003

Rhus verniciflua Stokes (RVS) has been used as a traditional herbal medicine. Several earlier studies indicated that an ethanol extract of RVS has both anti-oxidant and anti-tumor properties, although the mechanism for the activity remains to be elucidated. In this report, we prepared a highly purified ethanol extract from RVS, named REEE-1 ($\underline{R}$hus $\underline{e}$thanol $\underline{e}$luted $\underline{e}$xtract-1), and investigated the mechanism involved in its growth-inhibitory effect on the human B and T lymphoma cell lines, BJAB and Jurkat, respectively. Results from tritium uptake proliferation assays showed that the proliferative capacities of both BJAB and Jurkat cells were strongly suppressed in the presence of REEE-1. This was further confirmed through trypan blue exclusion experiments that revealed a dose-dependent decrease in viable cell numbers after REEE-1 treatment. REEE-1-mediated suppression of cell growth was verified to be apoptotic, based on the increase in DNA fragmentation, low fluorescence intensity in nuclei after propidium iodide staining, and the appearance of DNA laddering. In particular, REEE-1 exerted its anti-oxidant activity through the inhibition of hydroxyl radical-mediated degradation by iron ion chelation rather than direct scavenging of hydroxyl radicals. Furthermore, REEE-1 was revealed to be a potential scavenger of superoxide anions. Collectively, our findings suggest that REEE-1 is a natural anti-oxidant that could be used as a cancer chemo-preventive and therapeutic agent.

• Preventive Nutrition and Food Science
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• 제13권3호
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• pp.139-145
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• 2008

We located a group of healthy young males (aged $20{\sim}30$) who had been taking a high dose (more than 5 g) of vitamin C daily for more than one year. We observed that this vitamin C group had plasma levels of vitamin C that were more than three times that of the control group. The control group had not taken any additional vitamin C except for that included in their diets. But the vitamin C group showed significantly lower amounts of Cu/ZnSOD, catalase and glutathione-s-transferase and lower activities of glutathione peroxidase and glutathione reductase in erythrocyte lysates than the control group. However, there was no difference in the plasma levels of lipid peroxides between the two groups. These results suggest that vitamin C offsets its own contribution to anti-oxidant activity by repressing the expression of anti-oxidant enzymes and also excludes the possibility that vitamin C acts as a pro-oxidant in vivo.

• 대한화장품학회지
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• 제31권2호
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• pp.189-196
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• 2005

익모초(Leonurus sibiricus L)는 한국, 중국, 일본, 아시아 각지에 야생으로 자생하는 2년생 초본으로 꿀풀과(Labiatae) 식물로 높이 1.5${\~}$2m 정도 자란다. 약효로는 부인병의 각종 자궁질환, 활혈, 조경, 이뇨, 소증 효과는 물론 혈변, 혈뇨, 치질, 대${\cdot}$소변 불통 등의 치료제로 사용한다. 그 외에 고혈압, 강심작용, 항암연구도 활발하다. 익모초의 $CHC1_3$ 엑스와 MeOH 엑스를 $H_2O$, $30\%$ MeOH, $60\%$ MeOH, $100\%$ MeOH로 분획하였고, 이 5가지 분획물들에 대하여 activity guided fractionation 방법에 따라 DPPH free radical에 대한 scavenging activity와 LDL의 lipid peroxidation을 이용한 TBARS assay로 항산화 활성실험을 측정하였다. 그 결과 익모초의 MeOH 엑스는 $30\%$ MeOH, $60\%$ MeOH 분획물이 항산화 활성이 증가하였으며, Vitamin C와 비교해 볼 때 강한 활성을 나타내었다. 또한 pyrogaliol을 이용한 SOD 활성에서도 250 ppm에서는 Vitamin C 보다 우수한 유사 활성을 보였다. 이상과 같이 익모초로부터 비교적 강한 항산화 활성을 확인할 수 있어 천연항산화 기능성 화장품의 개발이 가능하리라 보여 진다.