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The Protective Effects of Ganoderma lucidum on the DNA Damage and Mutagenesis  

이길수 (한림대학교 자연과학대학 생명과학부)
공석경 (한림대학교 자연과학대학 생명과학부)
최수영 (한림대학교 자연과학대학 생명과학부)
Publication Information
Biomolecules & Therapeutics / v.11, no.2, 2003 , pp. 139-144 More about this Journal
Abstract
Ganoderma lucidum is commonly known as medically potent mushroom, which has been widely used in China and other oriental countries for the treatment of various diseases, including cancer. In this report, we investigated the anti-oxidant and protective effect of Ganodema lucidum extract (GLE) against the DNA damage induced by free radical and U.V. In the assay of cell growth inhibition, the inhibitory cell growth rate induced by hydroxyl radical was dose-dependently decreased by GLE. This results support that GLE has a detoxifying activity against cytotoxicity of hydroxyl radical in E. coli cell. GLE also protected ColE1 plasmid DNA damage in the concentration of 200$\mu\textrm{g}$ per reaction on the DNA fragmentation assay. The nuclear tailing by hydrogen peroxide in single cell gel electrophoresis(SCGE) was decreased by GLE in the concentration of 50$\mu\textrm{g}$/ml. These data indicate that Ganoderma lucidum has an anti-oxidative activity to hydrogen peroxide. The mutation rate after irradiation of U.V. was reduced by 50$\mu\textrm{g}$/ml GLE and total number of Rif (Rifampicin) resistant mutants was decreased in a concentration dependent manner when added the GLE exogenously in a culture media. According to the results, it is likely that GLE has not only an anti-oxidative activity to hydroxyl radical but also an anti-mutagenic activity to U.V. mutagenesis.
Keywords
Ganoderma lucidum; free radical; DNA damage; UV mutagenesis; anti-oxidant effect;
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1 Wang, S. Y., Hsu, M. L., Hsu, H. C., Tzeng, C. H., Lee, S. S., Shiao, M. S. and Ho, C. K. (1997). The anti-tumor effect of Ganoderma luciduni is mediated by cytokines released from activated macrophages and T lymphocytes. Int. J Cancer 70(6), 699-705   DOI   ScienceOn
2 Zhang, J., Tang, Q., Zimmerman-Kordmann, M., Reutter, W. and Fan, H. (2002). Activation of B lymphocytes by GLIS, a bioactive proteoglycan from Ganoderma lucidum. Life Sci. 71(6), 623-638   DOI   ScienceOn
3 Bao, X. F., Wang, X. S., Dong, Q., Fang, J. N., and Li, X. Y. (2002). Structural features of immunologically active polysaccharides from Ganoderma lucidum. Phytochemistry 59(2), 175-181   DOI   ScienceOn
4 Fridovich, I. (1978). The Biology of Oxygen Radicals. Science. 201, 875-880   DOI
5 Barbouti, A., Doulias, P, Nousis, L., Tenopoulou, M., and Galaris, D. (2002). DNA damage and apoptosis in hydrogen peroxide-exposed Jurkat cells: bolus addition versus continuous generation of H$_{2}O_{2}$. Free Radle Biol. Med. 33(5), 691   DOI   ScienceOn
6 Ding, W. X., Shen, H. M., Zhu, H. G., Lee, B. L., and Ong, C N. (1999). Genotoxicity of microcystic cyanobacteria extract of a water source in China. Mutat. Res. 442(2), 69-77   DOI   ScienceOn
7 Emcrit, J., Beaumount, C. and Trivin, F. (2001). Iron metabolism, free radicals, and oxidative injury, Biomed Pharmacothen 55, 333-339   DOI   ScienceOn
8 Halliwell, B. and Gutteridge, .J. M. C. (1989). Free radicals in biology and medicine. Oxford Science. New York, pp.86
9 Keycr, K., Gort A. S. and Imlay J. A. (1995). Superoxide and the production of Oxidative DNA damage. Jounal of Bacteriology. 177, 6782-6790   DOI
10 Kim, K. C. and Kim, I. G. (1999). Ganoderma lucidum extract protects DNA from strand breakage caused by hydroxyl radical and UV irradiation. Int. J. Mol. Med. 4(3), 273-277
11 Miller, J. H. (1992). A short course in bacterial genetics, CSHL Press, USA, pp.150
12 Olive, P. L., Wlodek, D., Durand, R. E. and Banath, J. P. (1992). Factors influencing DNA Migration from individual Cells Subjected to Gel Electrophoresis. Exp. Cell Res. 198, 259-267   DOI   ScienceOn
13 Sastre, J., Borras, C., Garcia-Sala, D., Lloret, A, Pallardo, F. V. and Vina, J.(2002). Mitochondrial damage in aging and apoptosis. Ann N.Y. Acad Sci. 959, 448-451   DOI
14 Shi, Y., James, A. E., Benzie, I. F. and Buswell, J. A. (2002). Mushroom-derived preparations in the prevention of H$_{2}O_{2}induced oxidative damage to cellular DNA Tetratog Carcinog Mutagen. 22(2), 103-111
15 Zhu, H. S., Yang, X. L., Wang L. B., Zhao, D. X. and Chen, L. (2000). Effects of extracts from sporoderm-broken spores of Ganoderma lucidurn on HcLa cells. Cell Biol. Toxicol. 16(3), 201-206
16 Shieh, Y. H., Liu, C. F., Huang, Y. K., Yang, J. Y., Wu, I. L., Lin, C. H. and Li S. C.(2001). Evaluation of the hepatic and renal-protective effcets of Ganoderma lwcidum in mice. Am. J. Chin. Med. 29(3-4), 501-507   DOI   ScienceOn
17 Sliva, Do, Labarrere, C., Slivova, V., Sedlak, M., Lloyd, F. P. Jr, and Ho, N. W. (2002). Ganoderma lucidum suppresses mobility of highly invasive breast and prostate cancer cells. Biochem. Biophvs. Res. Conunun. 298(4), 603-612   DOI   ScienceOn
18 Szweda, P. A., Friguet, B. and Szweda, L. I. (2002). Proteolysis, free radicals, and aging., Free Radic Biol. Med. 33(1), 29-36   DOI   ScienceOn
19 Keyer, K. and Imlay J, A. (1996). Superoxide accelerates DNA damage by elcvating free-iron levels. Proc. Natl. Acad. Sci. USA. 93, 13635- 13640   DOI   ScienceOn
20 Jong, S. C. and Birmingham J. M. (1992). Medicinal benefits of the mushroom Ganoderma: Adv. Appl. Microbiol. 37, 101-134   DOI