• 한국식품조리과학회지
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• 제29권6호
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• pp.653-660
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• 2013

Acaiberry(Euterpe oleracea Mart.) was partially substituted from the formula for juak and the subsequent effects on the physiochemical and physical characteristics. And this study was carried out to standardize the recipe of acaiberry-juak. Acaiberry powder was added in 0(control), 1, 3 and 5% substitution ratios by weight per glutinous rice flour. Also, acaiberry puree was added in 3, 6 and 9% substitution ratios by weight water. The moisture contents showed juak with addition of acaiberry contents were lower then control. The diameter of the juak decreased with increasing levels of the added acaiberry powder and puree, whereas the height increased with increasing amount of the powder and puree. The Hunter colorimeteric L-values of the dough and acaiberry-juak decreased as the amount of acaiberry powder and puree increased. Hunter a and b-values both increased with increasing levels of the acaiberry powder and puree. Textural characteristics there were increase in hardness, springiness, chewiness as the amount of acaiberry powder and puree increased(p<.001). Cohesiveness were not significantly different as the level of acaiberry powder and puree increased(p<.001). The anti-oxidant capacity results, anthocyanin, DPPH radical scavenging activity, SOD-like activity, Nitrate-scavenging ability tended to increased by according to the addition acaiberry powder and puree(p<.001). As measured DSC, the onset temperature and enthalpy were accordingly increased during storage period as the acaiberry contents were higher then control. According to sensory evaluation results, the after swallowing, appearance, flavor, taste and texture of juak was acaiberry powder 1% and acaiberry puree 6% proper score is recorded. Overall acceptability of acaiberry juak powder 1%, puree 6% percent of the additives are also the highest mark in the sensory characteristics. Also aciberry powder addition of more than demonstrate a good reputation in the development juak will suggest more appropriate form of puree.

• 한국응용과학기술학회지
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• 제32권1호
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• pp.16-22
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• 2015

빈카마이너 L.의 알칼로이드는 HPLC의 PDA 검출기를 이용하여 측정하고 이를 표준시료인 빈카민의 자외선/가시광선 흡수 스펙트럼과 비교하여 분석하였다. 플라보노이드류를 포함하여 추출물에서 9개 이상의 화합물에 대한 자외선/가시광선 흡수 스펙트럼을 얻었다. 추출방법에 따라 에탄올 추출물과 열수 추출물을 얻고 이를 DPPH 라디칼 소거법에 의해 항산화 효과를 조사하였다. 그 결과 에탄올 추출물과 열수 추출물 모두 비교군인 케르세틴과 비타민 C 대비 약 14~15%의 항산화 효과를 보여 추출법에 상관없이 유사한 결과를 보였다.

• 한국축산시설환경학회지
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• 제19권2호
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• pp.155-162
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• 2013

The objective of this study is to investigate the effects of Cynanchum wilfordii (CW) on cell viability, anti-oxidant activity, volatile fatty acid (VFA) production and methane gas production. Collected rumen fluid incubated with CW powder (1% w/v) for 12 and 24 hours were analyzed for pH, VFAs and methane. Alamar blue assay showed no significant difference on the viability of 3T3-L1 and C2C12 cells treated with CW for 24 hours. TBARS data showed a dose dependent increase on the antioxidant activity of CW. VFAs increased in the CW-treated groups compared to the control group. In addition, propionate increased more than other VFAs by the treatment with CW. There was a significant decrease in methane gas production in batch culture treated with CW in 12hrs. In conclusion, it was suggested that Cynanchum wilfordii could manipulate rumen fermentation considered by increasing VFA production and inhibition of methanogenesis.

• Biomolecules & Therapeutics
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• 제20권6호
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• pp.499-505
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• 2012

Chemoprevention represents a strategy designed to protect cells or tissues against various carcinogens and carcinogenic metabolites derived from exogenous or endogenous sources. Recent studies indicate that plant-derived triterpenoids, like oleanolic acid, may exert cytoprotective functions via regulation of the activity of different transcription factors. The chemopreventive effects may be mediated through induction of the nuclear factor erythroid 2-related factor 2 (Nrf2) transcription factor. Activation of Nrf2 by triterpenoids induces the expression of phase 2 detoxifying and antioxidant enzymes such as NAD(P)H quinone oxidoreductase 1 (NQO1) and heme oxygenase-1 (HO-1) - proteins which can protect cells or tissues against various toxic metabolites. On the other hand, inhibition of other transcription factors, like NF-${\kappa}B$ leads to the decrease in the pro-inflammatory gene expression. Moreover, the modulation of microRNAs activity may constitute a new mechanism responsible for valuable effects of triterpenoids. Recently, based on the structure of naturally occurring triterpenoids and with involvement of bioinformatics and computational chemistry, many synthetic analogs with improved biological properties have been obtained. Data from in vitro and in vivo experiments strongly suggest synthetic derivatives as promising candidates in the chemopreventive and chemotherapeutic strategies.

• 한국미생물·생명공학회지
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• 제33권3호
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• pp.231-235
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• 2005

RBL-2H3 cell에서 HRE에 의하여 histamine이 분비되는 과정에서 ROS가 생성되는지 실험해 본 결과, ROS가 HRF를 처리한지 5분대에 최대치를 보이며 생성되었다가 소멸되는 것을 관찰할 수 있었다. 따라서 HRF가 세포내 second messenger로써의 ROS를 생성하였다고 확인할 수 있었다. 또한 ROS는 단백질 정제 과정에서의 endotoxin오염에 의해 영향을 받지만,본 실험에서 규명한 HRF에 의한 ROS 생성은 endotoxin에 의한 것이 아닌, 순수하게 HRF에 의한 signaling의 결과라는 것도 확인할 수 있었다.

• The Korean Journal of Physiology and Pharmacology
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• 제10권2호
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• pp.79-83
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• 2006

We evaluated therapeutic and preventive properties of dehydroepiandrosterone (DHEA), a weak androgenic steroid, against isoproterenol-induced cardiomyopathy. The cardiomyopathy was induced by daily i.p. administration of isoproterenol to rats for five days. One group of rats were given with daily s.c. for 5 days during isoproterenol and the other group with daily s.c. DHEA for total 10 days, including 5 days before and during isoproterenol. The animals were killed after each treatment, and cardiac muscle failure was evaluated using histopathologic examination and biochemical indices. DHEA was found to reduce the damaged area and inhibit the elevation in the serum levels of glutamic oxaloacetic transaminase (SGOT), lactate dehydrogenase (LDH), skeletal muscle creatine kinase (CK) and heart creatine kinase (CK-MB) induced by isoproterenol. We also assayed widely used oxidative stress parameters, including thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), catalase and glutathion peroxidase (GPx). DHEA decreased the escalated level of TBARS and enhanced the anti oxidant defense reaction with an increase in Mn-SOD and Cu/Zn-SOD. On the other hand, the treatment with DHEA did not affect catalase and GPx activity. The present study indicates that DHEA has a therapeutic and preventive effect against isoproterenol-induced cardiomyopathy and its effects may depend largely on the increase in SOD activity.

• The Korean Journal of Physiology and Pharmacology
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• 제16권6호
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• pp.463-468
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• 2012

Type 1 diabetes (T1D) is caused by dysregulation of the immune system in the pancreatic islets, which eventually leads to insulin-producing pancreatic ${\beta}$-cell death and destabilization of glucose homeostasis. One of the major characteristics of T1D pathogenesis is the production of inflammatory mediators by macrophages that result in destruction or damage of pancreatic ${\beta}$-cells. In this study the inflammatory microenvironment of T1D was simulated with RAW264.7 cells and MIN6 cells, acting as macrophages and pancreatic ${\beta}$-cells respectably. In this setting, peroxiredoxin-1, an anti-oxidant enzyme was knocked down to observe its functions in the pathogenesis of T1D. RAW264.7 cells were primed with lipopolysaccharide and co-cultured with MIN6 cells while PRX-1 was knocked down in one or both cell types. Our results suggest that hindrance of PRX-1 activity or the deficiency of this enzyme in inflammatory conditions negatively affects pancreatic ${\beta}$-cell survival. The observed decrease in viability of MIN6 cells seems to be caused by nitric oxide production. Additionally, it seems that PRX-1 affects previously reported protective activity of IL-6 in pancreatic ${\beta}$ cells as well. These results signify new, undiscovered roles for PRX-1 in inflammatory conditions and may contribute toward our understanding of autoimmunity.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2003년도 제20차 정기총회 및 학술발표회
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• pp.73-74
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• 2003

육계 병아리에서 어유 함유사료 급여에 의한 급성기 반응중의 생산성 감소 완화와 단백질 대사, 에너지 대사 및 항산화효소 활성의 관계를 조사하였다. 병아리에 1일령부터 어유 사료를 급여하면 급성기 반응중에 에너지 밸런스를 높이나 성장률, 요산 배설량과 질소 밸런스가 낮아지지 않았으며, 대두유 함유 사료를 급여한 것에 비하여 간장과 적혈구 세포액의 SOD 활성을 높였다. 이러한 결과는 어유 함유사료 급여로 급성기 반응중의 증체량 감소가 완화되는 효과는 항산화효소계의 조정과 에너지 급원의 소화관내 흡수 증가에 기인한다는 것을 나타낸다.

• Clinics in Shoulder and Elbow
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• 제22권2호
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• pp.79-86
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• 2019

Background: Increased oxidative stress and inflammation play a critical role in the etiopathogenesis of chronic tendinopathy. Melatonin is an endogenous molecule that exhibits antioxidant and anti-inflammatory activity. The aim of this study was to evaluate the biochemical and histopathological effects of exogenous melatonin administrations in supraspinatus overuse tendinopathy. Methods: Fifty rats were divided into the following four groups: cage activity, melatonin treatment, corticosteriod therapy, and control. Melatonin (10 mg/kg, intraperitoneal; twice a day) and triamcinolone (0.3 mg/kg, subacromial; weekly) were administered to the treatment groups after the overuse period. Biochemical and histopathological evaluations were performed on serum samples and biopsies obtained from rats. Plasma inducible nitric oxide synthase (iNOS), vascular endothelial growth factor (VEGF), total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) levels were evaluated biochemically. Results: The TAS, TOS, OSI, iNOS, and VEGF values were significantly lower than the pre-treatment levels in rats receiving exogenous melatonin treatment (3 or 6 weeks) (p<0.05). TOS, iNOS, VEGF, and OSI values after 3 weeks of triamcinolone administration, and TOS, VEGF, and OSI levels after 6 weeks of triamcinolone application, were significantly lower than the pre-treatment levels (p<0.05). Conclusions: Exogenous melatonin application in overuse tendinopathy reduces oxidative stress and inflammation. Melatonin might be an alternative potential molecule to corticosteroids in the treatment of chronic tendinopathy.

• Journal of Nutrition and Health
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• 제47권6호
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• pp.394-402
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• 2014

본 연구는 황칠나무의 부위별 추출물의 항당뇨효능을 평가하기 위해, 황칠나무의 잎과 줄기의 열수추출물과 에탄올추출물을 분리하여 항산화능과 당뇨질환 개선효능을 측정하였다. 그 결과, total polyphenol과 total flavonoid의 농도는 DLW에서 가장 높게 나타났으며, 다음으로 DLE, DSW, DSE 순서로 나타났다. 또한, DPPH 소거능은 DLW가 대부분의 농도에서 가장 높게 나타났으며, DLE는 $500{\mu}g/mL$의 농도에서 가장 높은 DPPH 소거능을 나타내었다. 항산화능이 우수한 DLW와 DLE를 선정하여 STZ로 유도된 당뇨모델동물에 2주간 투여하여 항당뇨 효능을 분석하였다. 그 결과, 체중은 STZ/vehicle 처리군이 급격히 감소하였으나 STZ/DLW와 STZ/DLE 처리군에서 서서히 감소하였다. 혈당량은 STZ/vehicle 처리군에서 급격히 증가하였으며, STZ/DLW 처리군과 STZ/DLE 처리군에서만 유의적인 감소를 나타내었다. 그러나, 혈청 내 인슐린의 농도는 STZ/vehicle 처리군에 비하여 STZ/DLW와 STZ/DLE 처리군에서 증가하였으며, 특히 STZ/DEL-200 처리군에서 가장 높은 농도를 나타내었다. 이러한 변화는 췌장의 조직학적 변화와 면역염색의 결과에서도 동일하게 관찰되었다. 더불어, 뇨당 농도는 STZ/vehicle 처리군에서 급격히 증가하였으며, 오직 STZ/DLW-200 처리군과 STZ/DLE-200 처리군에서만 유의적으로 감소하였다. 이러한 결과는 황칠나무의 잎의 열수추출물과 에탄올추출물은 우수한 항산화효능과 베타세포의 사멸을 억제함으로서 당뇨질환을 개선하는 효능이 있음을 제시하고 있으며, 특히, 200 mg/kg의 농도가 우수한 효능을 나타내는 농도임을 제시하고 있다.