• 동의생리병리학회지
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• 제28권5호
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• pp.506-511
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• 2014

Commelina communis Ledeb is a widely used medication for the treatment of antidiabetic, antioxidant and hypoglycemic agent in Korea. Alpha-melanocyte stimulating hormone (${\alpha}$-MSH) is a major factor to stimulate melanin synthesis in the skin. The purposes of this study was to investigate the inhibitory effects of extract from Commelina communis Ledeb (ECC) on ${\alpha}$-MSH-stimulated melanogenesis in B16F10 cells. ECC suppressed melanin synthesis and intracellular tyrosinase activity in B16F10 cells or ${\alpha}$-MSH-induced B16F10 cells in a dose dependent manner. In study on the melanogenic protein expressions, it had especially influence on expressions of tyrosinase and tyrosinase-related protein (TRP-1). Tyrosinase and TRP-1 expressions were gradually decreased in a dose-dependent. Additionally, the extract also decreased the ${\alpha}$-MSH-induced over-expression of tyrosinase and TRP-1. This results show that the anti-melanogenic activity of ECC is correlated with the suppression of tyrosinase and TRP-1 protein expressions in B16F10 cells.

• 생약학회지
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• 제47권4호
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• pp.343-351
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• 2016

The purpose of this study was to evaluate the whitening effect of aerial part of Pueraria lobata and mechanisms. Aerial part of Pueraria lobata, dose-dependently reduced the melanin content. Aerial part of Pueraria lobata, significantly decreased cellular tyrosinase activity, while there was not any effect on tyrosinase in cell-free conditions. To elucidate the mechanisms behind the aerial part of Pueraria lobata, treated melanogenesis regulation, the expressions of melanogensis related genes, proteins, and the activity of ${\alpha}-glucosidase$ were determined. Aerial part of Pueraria lobata, significantly inhibited gene and protein levels of MITF, tyrosinase and TRP-1. It suppressed the ${\alpha}-glucosidase$, leading to inhibition on the maturation of tyrosinase. Also aerial part of Pueraria lobata, was observed to have the high antioxidant activity. These results suggested that whitening effect of aerial part of Pueraria lobata, should be due to the down-regulation of MITF, tyrosinase and TRP-1 expression and the intercepting maturation of tyrosinase through suppressing ${\alpha}-glucosidase$. Another should be the high anti-oxidant activity. The findings show the possibility that aerial part of Pueraria lobata, can be used as a potential skin-whitening agent.

• Journal of Microbiology and Biotechnology
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• 제28권3호
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• pp.375-380
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• 2018

We have previously found that mycelia culture broth of eight kinds of traditional herbal extracts fermented with Phellinus linteus (previously named as 8-HsPLCB) not only inhibited melanin and tyrosinase activity, but also reduced the contents of melanogenesis-related proteins, including tyrosinase and microphthalmia-associated transcription factor, in 3-isobutyl-1-methylxanthine-stimulated B16F0 melanoma cells. For a further study, the effect of 8-HsPLCB against skin pigmentation in brown guinea pigs with ultraviolet B (UVB)-induced hyperpigmentation was investigated. 8-HsPLCB (3%) and arbutin (2%) as positive controls were applied topically twice daily for 4 weeks to the hyperpigmented areas. 8-HsPLCB showed skin-lightening effect as effective as arbutin, one of the most widely used in whitening cosmetics. Melanin index values as the degree of pigmentation showed a significant reduction week by week post 8-HsPLCB treatment and then substantially reduced by 4 weeks. The degree of depigmentation after 4 weeks of topical application with 8-HsPLCB was 32.2% as compared with before treatment (0 week). Moreover, using Fontana-Masson staining and hematoxylin-eosin staining, 8-HsPLCB reduced melanin pigmentation in the basal layer of the epidermis and epidermal thickness changes exposed to the UV-B irradiation as compared with non-treatment and vehicle treatment. The intensity of the skin-lightening effect of 8-HsPLCB was similar to arbutin. These results suggest that the skin-lightening effect of 8-HsPLCB might be resulted from inhibition of melanin synthesis by tyrosinase in melanocytes. To conclude, 8-HsPLCB treatment showed reduction of the melanin pigment and histological changes induced by UV irradiation in brown guinea pigs.

• 한국응용과학기술학회지
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• 제34권3호
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• pp.623-630
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• 2017

본 연구는 구기자 종자를 초임계유체 추출한 후 in vitro 미백활성과 항균 실험을 실시하였으며, 추출물을 에멀션에 적용하여 안정성 평가를 수행하기 위해 pH, 점도, 에멀션 입자를 관찰하였다. 그 결과 미백활성의 경우 $750{\mu}g/mL$에서 81.86 %의 미백 활성을 나타내었으며, 항균효과의 경우 Staphylococcus epidermidis, Candida albicans, Escherichia coli 균에서 항균효과가 나타났다. 또한 추출물을 함유한 에멀션 물리적 안정성 평가에서 점도와 pH가 28일 동안 큰 변화가 없었으며 유화물 입자 관찰 시 경시변화에 따른 합일과 분리현상이 확인되지 않았다. 이러한 결과는 화장품소재로서의 구기자 씨앗 추출물이 유용되게 활용될 수 있는 가능성을 가진 것으로 평가된다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권8호
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• pp.3795-3802
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• 2012

Cochinchina momordica seeds (CMS) have been widely used due to antitumor activity by Mongolian tribes of China. However, the details of the underlying mechanisms remain unknown. In the present study, we found that an EtOAc (ethyl ester) extract of CMS (CMSEE) induced differentiation and caused growth inhibition of melanoma B16 F1 cells. CMSEE at the concentration of $5-200{\mu}g/ml$ exhibited strongest anti-proliferative effects on B16 F1 cells among other CMS fractions (water or petroleum ether). Moreover, CMSEE induced melanoma B16 F1 cell differentiation, characterized by dendrite-like outgrowth, increasing melanogenesis production, as well as enhancing tyrosinase activity. Western blot analysis showed that sustained phosphorylation of p38 MAP accompanied by decrease in ERK1/2 and JNK dephosphorylation were involved in CMSEE-induced B16 F1 cell differentiation. Notably, 6 compounds that were isolated and identified may be responsible for inducing differentiation of CMSEE. These results indicated that CMSEE contributes to the differentiation of B16 F1 cells through modulating MAPKs activity, which may throw some light on the development of potentially therapeutic strategies for melanoma treatment.

• 한국식품과학회지
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• 제49권5호
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• pp.519-523
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• 2017

본 연구에서 공심채(Ipomoea aquatica)에 대한 다양한 생리활성을 조사하여 기능성소재 응용가능성을 검토하였다. 공심채 추출물은 멜라노마 세포에 대하여 낮은 세포독성을 나타냈다. 세포독성이 거의 없는 농도에서 공심채 추출물 처리 시, 산화방지 활성($ID_{50}$, $7.84{\mu}g/mL$), 타이로시네이스 활성저해($ID_{50}$, $106.56{\mu}g/mL$) 및 멜라닌 함량 저하($ID_{50}$, $41.75{\mu}g/mL$)를 보여주었다. 공심채 추출물 농도 의존적으로 타이로시네이스 발현이 억제되었으며, 이는 공심채 추출물이 직접적인 타이로시네이스 활성저해 및 세포 내 타이로시네이스 발현을 억제시킴으로써 멜라닌 합성을 저해하는 것으로 판단된다. 또한, 공심채 추출물이 피부장벽 보호 지표물질로서 관련된 단백질인 인볼루크린 발현을 증가시키는 것을 확인하였다. 이와 같은 결과로 미루어 볼 때 공심채 추출물은 피부미백 소재 등 피부장벽 개선 효과를 지닌 기능성 화장품에 활용하기 위한 매우 효과적인 재료가 될 수 있다고 판단된다.

• 한국약용작물학회지
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• 제19권3호
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• pp.177-184
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• 2011

This study was performed to assess the antioxidant activities and whitening effects of Agrimonia pilosa Ledeb on melanin synthesis. The whitening effects of Agrimonia pilosa Ledeb water extracts were examined by in vitro mushroom tyrosinase assay and B16BL6 melanoma cells. We assessed inhibitory effect of Agrimonia pilosa Ledeb water extract on expression of melanogenic enzyme proteins including tyrosinase, tyrosinase-related protein 1 (TRP-1) and tyrosinase-related protein 2 (TRP-2) in B16BL6 cells. Inhibitory effect of Agrimonia pilosa Ledeb onto free radical generation was determined by measuring DPPH and hydroxyl radical scavenging activitie. Our results indicated that Agrimonia pilosa Ledeb water extract effectively inhibited free radical generation. In DPPH and hydroxy radical scavenging activity, Agrimonia pilosa Ledeb water extract had a potent anti-oxidant activity in a dose-dependent manner. They significantly inhibited tyrosinase activity in vitro and in B16BL6 melanoma cells. Also, Agrimonia pilosa Ledeb suppressed the expression of tyrosinase in B16BL6 melanoma cells. These results show that Agrimonia pilosa Ledeb inhibited melanin production on the melanogenesis. The underlying mechanism of Agrimonia pilosa Ledeb on whitening activity may be due to the inhibition of tyrosinase activity. We suggest that Agrimonia pilosa Ledeb may be useful as new natural active ingredients for antioxidant and whitening cosmetics.

• 한국약용작물학회지
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• 제19권4호
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• pp.264-270
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• 2011

In this study, we investigated the cosmetic application of Acer mono sap through an ultra-high pressure process. Exposing Acer mono sap to a ultra-high pressure process resulted in 90.1% cell viability of human normal fibroblast cells (CCD-986sk) when added at the highest concentration. Acer mono sap also showed the hightest free radical scavenging activity after the ultra high pressure process. The melanogenesis inhibition rate in cloned M-3 cells was 59.0%. Tyrosinase was inhibited at a rate of 87.2% by adding 100% HPAMS. Anti-wrinkle activity was 78.1%. Acer mono sap showed enhanced storage following the ultra high pressure process. These results indicate that Acer mono sap may be a source for functional cosmetic agents capable of improving antioxidant, whitening, and antiwrinkling effects.

• 한국식품과학회지
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• 제50권3호
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• pp.344-348
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• 2018

본 연구에서 오크라(Abelmoschus esculentus)에 대한 다양한 생리활성을 조사하여 기능성소재 응용가능성을 검토하였다. 오크라 추출물은 멜라노마 세포에 대하여 낮은 세포독성을 나타냈다. 세포독성이 거의 없는 농도에서 오크라 추출물 처리 시, 산화방지활성($ID_{50}$, $5.24{\mu}g/mL$), tyrosinase 활성저해($ID_{50}$, $102.12{\mu}g/mL$) 및 멜라닌 함량 저하($ID_{50}$, $17.85{\mu}g/mL$)를 보여주었다. 오크라 추출물 농도 의존적으로 tyrosinase 발현이 억제되었으며, 이는 오크라 추출물이 직접적인 tyrosinase 활성저해 및 세포 내 tyrosinase 발현을 억제시킴으로써 멜라닌 합성을 저해하는 것으로 판단된다. 또한, 오크라 추출물이 피부장벽 보호 지표물질로서 관련된 단백질인 involucrin 발현을 증가시키는 것을 확인하였다. 이와 같은 결과로 미루어 볼 때 오크라 추출물은 피부미백 소재 등 피부장벽 개선 효과를 지닌 기능성 화장품에 활용하기 위한 매우 효과적인 재료가 될 수 있다고 판단된다.

• 한국식품과학회지
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• 제51권1호
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• pp.52-57
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• 2019

본 연구는 그라비올라 잎 조다당 분획물(ALP)의 멜라닌 저해활성에 관하여 알아보기 위하여 B16F10 melanoma 세포의 세포 생존율, tyrosinase 저해활성, 멜라닌 함량, 멜라닌 생합성에 관여하는 단백질(TRP-1, TRP-2, tyrosinase, MITF 및 p-CREB)의 발현을 관찰하였다. ALP의 tyrosinase 활성 저해능을 측정하였을 때, 농도 의존적으로 감소하는 것을 알 수 있었다. 또한 B16F10 세포에 ALP를 처리하여 멜라닌 활성 저해능에 관하여 알아봤을 때, tyrosinase, 멜라닌 함량이 농도 의존적으로 줄어드는 것을 확인하였다. 또한 ALP가 미백과 관련된 TRP-1, TRP-2, tyrosinase, MITF 및 p-CREB의 단백질 발현양이 농도의존적으로 감소되는 것으로 관찰되었다. 따라서 ALP는 멜라닌 저해 활성을 효과적으로 나타낸 것으로 보아 기능성 미백 화장품 소재로서의 활용 가능성을 확인할 수 있었다.