• Korean Chemical Engineering Research
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• 제62권2호
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• pp.142-146
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• 2024

전세계적으로 천연화장품에 대한 관심과 수요가 크게 늘어나고 있다. 따라서 기존 화학합성 기능성화장품소재를 대체하기 위한 노력의 일환으로서 천연물에서 기능성화장품소재를 발굴하고자 하였다. 모자반은 우리나라의 해안 전지역에서 쉽게 볼 수 있는 갈조류의 일종으로서 식용으로도 이용되는 만큼 안전성이 확보되어있는 해조류이다. 본 연구에서는 큰잎 모자반(Sargassum coreanum). 짝잎 모자반(Sargassum hemiphyllum), 쌍발이 모자반(Sargassum patens)의 3가지 종류의 모자반으로부터 열수를 이용하여 유효성분을 추출하였으며, 항산화효과, 미백효과, 주름개선효과, 자외선흡수효과 및 항염증효과를 측정하여 기능성 화장품 소재로서의 응용 가능성을 살펴보고자 하였다. 모자반추출물은 세포독성시험결과 안전성이 매우 우수하였고, DPPH radical scavenging activity를 이용한 항산화능 측정결과 우수한 항산화능을 보여주었으며 tyrosinase inhibition을 이용한 미백효과시험에서는 큰잎 모자반(Sargassum coreanum)의 미백효과가 상당히 우수하였다. 그러나 주름개선효과, 자외선흡수효과와 항염증효과는 비교적 낮았다. 결론적으로 큰잎 모자반(Sargassum coreanum)은 세포독성이 적었고, 항산화효과와 미백효과가 우수하여 미백 기능성화장품소재로서 응용가능성이 높음을 알 수 있었다.

• 한국식품과학회지
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• 제53권2호
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• pp.213-217
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• 2021

Benign prostate hyperplasia (BPH) is one of the most common elderly disease, and because of prolonged incubation period and many side effects of medication or surgical interventions, the use of dietary phytochemicals is considered as an effective measure for prevention of BPH. The purpose of this study is to investigate the mechanism of inhibition effect for BPH by flavonoids such as baicalein and kaempferol. BPH cells were collected through biopsy from patients with PSA of 4 or higher, followed by primary culture. In vitro experiments were conducted to evaluate mitochondrial respiration, intracellular reactive oxygen species (ROS) level and expression of inflammatory markers, genes, and anti-oxidants. In conclusion, baicalein and kaempferol have been demonstrated to inhibit BPH through lowering ROS, thereby reducing inflammation triggers, and reduced inflammation. This study is expected to be helpful in the development of flavonoids that have a clinical effect on suppressing BPH.

• 대한화장품학회지
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• 제32권1호
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• pp.23-28
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• 2006

본 연구는 택사의 화장품 원료로서의 특성을 알아보기 위하여 화장품의 기능들인 항산화, 미백, 세포 손상 회복 및 항염증과 관련된 다양한 실험을 실시하였다. 30, 70, 100% MeOH 용매로 추출한 택사 추출물들은 DPPH법으로 실시한 free radical scavenging assay에서 좋은 활성을 보여주었고, tyrosinase 활성 억제 시험에서도 0.5% 이상의 농도에서 농도 의존적인 활성을 보여주었다. Human fibroblast를 사용한 proliferation assay (MTT assay)에서 각 용매 추출물들은 별다른 효과를 보여주지 못했고 0.05% 이하의 농도에서는 세포 독성으로부터 안전하다는 것을 알 수 있었다. Bl6 melanocyte를 사용한 melanin 생성 억제시험에서 각 용매별 추출물은 독성으로부터 안전한 0.05% 이하의 농도에서 melanin 생성을 40% 이상 억제하는 높은 활성을 보여주었다. 이후 우리는 택사 추출물의 MPLC분리 분획을 실시하여 세 가지 분획을 얻었으며 이들을 대상으로 세포 손상 회복시험과 melanin생성 억제 시험, 염증인자 생성 억제 시험을 실시하였다. 그 결과 분획물들 중 3번 분획물이 세포 손상 회복을 30% 이상 올려주는 좋은 결과를 보여주었고, melanin 생성 억제 시험과 COX-2 생성억제에서도 주목할만한 결과를 보여주었다.

• 한국미생물·생명공학회지
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• 제38권4호
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• pp.434-441
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• 2010

최근 연구에서 폴리페놀을 함유한 천연물은 질병의 위험과 노화의 원인인 ROS와 RNS를 감소시키는 것과 연관이 있다고 밝혔다. 본 연구에서는 섬쑥부쟁이 추출물과 분획물의 산화와 염증에 대한 저해 효과가 있는지에 대한 연구를 조사하였다. 섬쑥부쟁이 70% 메탄올 추출물은 헥산층, 에틸아세테이트층, 부탄올층, 물층을 극성별로 분획하였다. 섬쑥부쟁이로부터 분획한 에틸아세테이트 추출물과 부탄올 추출물의 전자공여능은 $50\;{\mu}g/mL$에서 각각 58.0%, 46.4%를 나타냈다. 에틸아세테이트 추출물의 superoxide anion radical 소거능은 $50\;{\mu}g/mL$에서 64.65%를 나타냈고, 부탄올 추출물은 $50\;{\mu}g/mL$의 농도에서 35.66%의 저해 효과를 나타내었다. 항염증 효과와 관련된 hyaluronidase와 lipoxygenase의 저해 활성에 대한 에틸아세테이트 분획물의 효과는 $5\;{\mu}g/mL$의 농도에서 각각 24.37%, 29.5%의 저해 효과를 나타냈다. 이러한 에틸아세테이트 추출물의 항염증 효과는 nitric oxide 생성 억제에서도 유의했으며, Raw 264.7 cell 내 LPS에 의해 유도된 iNOS 단백질에서 $50\;{\mu}g/mL$의 EtOAc 추출물을 처리시 81.5%의 발현을 억제하는 효과가 확인되었다.

• 한방안이비인후피부과학회지
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• 제31권4호
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• pp.22-30
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• 2018

Objectives : The aim of this study is to investigate the relationship between chronic low grade inflammation theory, psoriasis, and herbal medicine. Methods : We reviewed recent studies on the relationship between chronic low-grade inflammation, psoriasis, and herbal medicine through Pubmed. Results : The pathological basis for psoriasis is the action of inflammatory mediators by the activation of the immune response, which can be a cause of various cardiovascular, metabolic and psychological symptoms of psoriasis patients, in addition to skin lesions. The herbal medicines improve these inflammatory conditions and improve local lesions through herbal medicine such as Qingdai, which have a strong inhibitory effect on IL-17,22 production. Conclusions : Herbal medicines used in psoriasis are thought to be effective not only for the improvement of local psoriasis lesions through anti-inflammatory effect but also for the improvement of systemic inflammation associated with chronic low grade inflammation.

• 대한한방내과학회지
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• 제43권1호
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• pp.68-78
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• 2022

Objective: In this study, we investigated the protective effect of rhododendron mucronulatum extract (RME) on allergic reactions and inflammation. Methods: The effect of RME was determined using ELISA and RT-PCR in RBL-2H3 mast cells and RAW 264.7 macrophage cells. We determined cell viability, β-hexosaminidase release, and the synthesis of IL-4 and TNF-α in RBL-2H3 cells. In addition, we determined NO from RAW 264.7 and the gene expression of IL-1β, iNOS, IL-6, TNF-α, and IL-10. Results: RME inhibited β-hexosaminidase release and synthesis of IL-4 and TNF-α in RBL-2H3 by the anti-DNP IgE plus DNP-HSA stimulation. In addition, RME inhibited the production of NO and the gene expression of IL-1β, iNOS, IL-6, and TNF-α in LPS-stimulated RAW 264.7 cells. Conclusion: From these results, we concluded that RME possesses anti-allergic activity and anti-inflammatory activity due to the inhibition of mast cells and macrophage function.

• 동의생리병리학회지
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• 제31권4호
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• pp.220-225
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• 2017

Lithospermi Radix (LR) is known to have an anti-inflammatory effect. However, the mechanisms are not well known. In this study, LPS-induced mouse RAW 264.7 macrophage cells were treated with LR to investigate the time-dependent inflammation response of LR. RAW 264.7 cells were treated with various concentrations of LR for 24 hours, followed by MTS assay. Cell viability was increased at all experimental concentrations. The mRNA expression levels of $IL-1{\beta}$, $TNF-{\alpha}$ and iNOS were increased by treatment of RAW 264.7 cells with LR at a concentration of $200{\mu}g/ml$ for 6 hours and 24 hours. Treatment of LR with $200{\mu}g/ml$ concentration for 6 hours promoted mRNA expression levels of $IL-1{\beta}$, $TNF-{\alpha}$ and iNOS in LPS-induced RAW 264.7 cells. However, $IL-1{\beta}$, $TNF-{\alpha}$ and iNOS mRNA expression was suppressed by treatment of LR with $200{\mu}g/ml$ concentration for 24 hours in LPS-induced RAW 264.7 cells. These results suggest that the effect on inflammation of LR is promptly promoted and then to rapidly alleviate the inflammatory reaction. This study proposes that the time-dependent activities of herbal medicine is a very important factor in analyzing the anti-inflammatory effect of various herbal medicines including LR.

• 대한본초학회지
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• 제31권5호
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• pp.79-84
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• 2016

Objective : Nypa fruticans Wurmb. Fruit (NF) has been used as a conventional medicine to treat inflammatory peridontal diseases in Myanmar and Eastern Asia. However, the anti-inflammatory effect of NF aqueous extract on lipopolysaccharide (LPS)-induced inflammatory responses was not well-investigated. Therefore, this study was aimed to investigate the anti-inflammatory effect of NF on LPS-induced inflammatory responses on RAW 264.7 cells.Methods : To induce inflammation on the macrophage cell line, RAW 264.7 cells were treated with 500 ng/mL of LPS. Water extracts of NF was treated 1 h prior to treatment of LPS. Cell viability was measured by MTT assay. Production of nitrite was measured with Griess assay and pro-inflammatory cytokines such as interleukine (IL)-1β and IL-6, and tumor necrosis factor (TNF)-α was measured by enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (PCR). In addition, we examined the inhibitory mechanisms of NF by western blot and immunocytochemistry.Result : Water Extract from NF itself did not have any cytotoxic effect at the concentration of 200 ㎍/ml in RAW 264.7 cells. Treatment of NF inhibited the production of nitrite, and pro-inflammatory cytokines inlcuding IL-1β, IL-6 and TNF-α in a dose dependant. In addition, NF treatment inhibited the LPS-induced activation and translocation of nuclear factor (NF)-κB.Conclusion : In summary, our result suggest that treatment of NF could reduce the LPS-induced inflammatory responses via deactivation of NF-κB. This study could suggest that NF could be a beneficial drug or agent to prevent inflammation.

• The Journal of Korean Physical Therapy
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• 제15권2호
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• pp.182-195
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• 2003

This study investigated the effects of triamcinolone acetonide by iontophoretic transdermal drug delivery on anti-inflammatory action into the rats and which had carrageenan-induced hyperalgesia and edema in the feet, trauma-induced tissue damage in the thigh. Each group was treated under the fellowing conditions. 1. Group I : Control group 2. Group II : Application of direct current 3. Group III : Application of 0.1$\%$ triamcinolone acetonide solution 4. Group IV : Iontophoresis of 0.1$\%$ triamcinolone acetonide solution The degree of anti-inflammation was evaluated by the paw withdrawal latency, the change in volume of foot the change of paw edema, histological change in rats. 1. In paw withdrawal latency, group IV showed the most significant therapeutic effect than the other groups at 0, 3, 6 and 9 hours(p < 0.001). 2. In paw edema experiment in the foot, group IV showed the most significant effect than group I at 0, 3, 6 and 9 hours. It meant that there was effective anti-inflammatory reaction in group I (p < 0.001). 3. In the light microscopic observation, group IV showed the most significant reduction of haemorrhage, hyperemia and infiltrative inflammation. From the results, the iontophoresis with triamcinolone acetonide is more effective than using each groups. It is one of the effective physical agent which delivered large molecular weight drug into the body. The continuous study is needed for many interesting issues of iontophoretic transdermal drug delivery in new future.

• 한방재활의학과학회지
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• 제23권1호
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• pp.25-49
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• 2013

Objectives : This study was carried out to know the anti-osteroarthritic effects of Bangkibokryeong-tang(Fanjifuling-tang(BBT)) on the papain-induced osteoarthritis C57BL/10 mouse. Methods : Osteoarthritis was induced by injection of papain(6 ${\mu}l$) into knee joint of mouse. Osteoarthritic mice were divided into 4 groups(normal, control, joins(R), BBT). The injection did not fit the normal group. A week later, after the injection of papain, control group was taken normal saline 200 ${\mu}l$, positive control group was taken joins(R)(100 mg/kg), treated group was taken extract of Bangkibokryeong-tang(Fanjifuling-tang(BBT))(400 mg/kg). After then, we examined hepatotoxicity, nephrotoxicity, inflammation cytokines, expression of inflammation factor mRNA, hemotology, histology through the micro CT-arthrography, and etc. Results : 1. Hepatotoxicity and nephrotoxicity have not expressed. 2. The levels of IL-$1{\beta}$, TNF-${\alpha}$, IL-6, MCP-1, Thromboxane B2, Leukotriene B4, Prostaglandin E2 in serum were significantly decreased. 3. In hematology, the levels of neutrophils and monocytes were significantly decreased. 4. The expression of inflammation factor mRNA like TNF-${\alpha}$ and IL-6, COX-2, iNOS-II were significantly inhibited. 5. In micro CT-arthrography, cartilage volume was less decreased. 6. The degree of osteoarthritis induced damage of joint of BBT group is low in histopathologic observation(hematoxylin&eosin(H&E), Safranin-O). Conclusions : According to this study, BBT has effect of anti-osteoarthritis. Further clinical research for the cartilage protective effect is necessary.