• Fisheries and Aquatic Sciences
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• v.21 no.8
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• pp.22.1-22.7
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• 2018

Background: Apoptosis is a process of programmed cell death, and apoptosis defect results in serious diseases such as cancer. Apoptosis induction is one of the key mechanisms of anti-cancer agents. This study was aimed to find anti-prostate cancer compounds from marine-derived fungus Microsporum sp. Results: We found that physcion isolated from the fermentation broth extract of the marine fungus Microsporum sp. strain MFS-YL decreases the cell proliferation of PC3 human prostate cancer cells. Physcion induced cell apoptosis as determined by Annexin V/propidium iodide double staining. Physcion downregulated the anti-apopotoic proteins such as Ras, Bcl-xL, and Bcl-2, whereas upregulated the pro-apoptotic Bax. Physcion also activated caspase-3, caspase-8, and caspase-9. Conclusion: These results suggest that physcion from Microsporum sp. inhibits the proliferation of PC3 human prostate cancer cells via the pathway leading to apoptotic cell death. Physcion may be a potential candidate in the field of anticancer drug discovery against human prostate cancer.

• Journal of the Korean Recycled Construction Resources Institute
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• v.2 no.2
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• pp.93-100
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• 2006

Building materials are trending toward environmental materials nowadays and the market share of those is growing. So those researches and developments for environmental property are proceeding now. The main properties of environmental products are far infrared emission, negative ion emission, electro magnetic wave shielding, and anti fungus, these products are used with shape of mortar, and spray on the finish material. But There are not much researches for the concrete, main material in construction field, with those functional properties. So in this research we evaluated slump, compressive strength and air content as basic properties for concrete using functional materials of sericite, wood pattern sand stone, carbon black and nanometric silver solution and functional properties like far infrared emission, negative ion emission, electro magnetic wave shielding, and anti fungus. The results were as follows. The most useful material in the functional materials was carbon black. Sericite and nanometric silver solution had a little effect on functional property, so it was difficult to apply to concrete, and wood pattern sand stone had a high functional property but low compressive strength, can be applied to a factory product. Anti fungus of the concrete using nanometric silver solution was not clear but if those specimens were aged in $CO_2$ gas for a long time it might apparent.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2014.11a
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• pp.65-67
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• 2014

There are many elastomeric joint sealant applications in construction such as structural glazing, weatherproofing and insulating glass fabrication etc. Each sealant joints require unique durability functions to perform well through building life cycle. Elastomeric joints in bathroom and kitchen is one of areas which require durable sealing. In this application, anti fungal durability is proprietary function of sealant during building life cycle. Premature failure of anti fungus resistance of sealant is putting big stresses to general contractor as well as the inhabitants due to costly rework and poor sanitation. Accordingly, when chemists design a product, they must take into account various parameters not only formulation components also test conditions in order to have long term fungal durability. This paper reviews several biocide options with various industry standards for fungus resistance performance to suggest making new test method for construction sealant industry.

• Journal of Life Science
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• v.29 no.4
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• pp.470-477
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• 2019

White jelly fungus (Tremella fuciformis Berk) is effective for hypertension, diabetes, obesity, lung disease, beauty, and has been known as an elixir of life in ancient Chinese dynasty. In this study, we investigated the anti-obesity and diabetic effects of the fermented white jelly fungus extract by the bioconversion process. We fed an obesity-inducing mouse with 5% non-fermented Tremella fuciformis (TF), 2.5% fermented Tremella fuciformis (FTF), and 5% FTF containing High Fats Diet (HFD) and HFD for 8 weeks, respectively. The oral glucose tolerance test (OGTT) was performed analysis after 7 weeks of feeding and the dietary intake, food efficiency ratio, body weight, liver, epididymal fat weight, and serum insulin level were measured after 8 weeks of feeding. Also, HOMA-IR was analyzed. The concentrations of serum total cholesterol, triglycerides test was analyzed. The FTF compare with 5% TF and HFD confirmed that 5% FTF reduced body weight, tissue weight, triglycerides concentration, HOMA-IR, respectively. As a result, we confirmed that the fermented white jelly fungus has the anti-obesity effect. Finally, this study can be used a basic data for obesity treatment using fermented white jelly fungus.

• The Plant Pathology Journal
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• v.19 no.2
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• pp.75-78
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• 2003

A lichen-forming fungus was successfully isolated by discharged spore method from Korean lichen (Hetero-dermia sp.) and cultivated in pure culture. The isolate JR0012 inhibited mycelial growth of several plant-pathogenic fungi. Mycelial growth of the four Pythium spp. tested was completely inhibited. Potato dextrose broth was found to be the medium favorable for large-scale production of antibiotics from the isolate. Anti-fungal substances produced in axenic culture were partially purified. This is the first report in Korea of lichen-forming fungus successfully isolated and which exhibited strong antifungal activity against plant-pathogenic fungi, especially the four Pythium spp..

• Mycobiology
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• v.47 no.4
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• pp.483-493
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• 2019

Antrodia cinnamomea is a unique medicinal fungus in Taiwan. It has been found rich in some pharmacologically active compounds for anti-cancer, hangover, and immune regulation etc. With the in-depth study of these components, it would be interesting and important to establish a molecular system for basic studies of A. cinnamomea. Thus, we would like to set up a foundation for this purpose by studying the A. cinnamomea protoplast preparation and regeneration. Firstly, we studied the optimization method of protoplast preparation of A. cinnamomea, and found various factors that may affect the yield during protoplast preparation, such as mycelial ages, pH values, and osmotic stabilizers. Secondly, in the regeneration of protoplasts, we explored the effects of various conditions on the regeneration of protoplasts, including different media and osmotic pressure. In addition, we found that citrate buffer with pH value around 3 dramatically increased the regeneration of protoplasts of A. cinnamomea, and provided a set of regeneration methodology for A. cinnamomea.

• Bulletin of the Korean Chemical Society
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• v.35 no.10
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• pp.3059-3062
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• 2014

Two new butyrolactones, asperphenol A (1) and B (2), together with four known butyrolactones (3-6) were isolated from the fermentation products of an endophytic fungus Aspergillus versicolor. Their structures were elucidated by spectroscopic methods including extensive 1D- and 2D-NMR techniques. Compounds 1-6 were also tested for their anti-tobacco mosaic virus (anti-TMV) activities. The results showed that compound 2 exhibited high anti-TMV activity with inhibition rate of 46.7%. The other compounds also exhibited potential anti-TMV activities with inhibition rates in the range of 21.8-28.4%.

• YAKHAK HOEJI
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• v.49 no.2
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• pp.140-146
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• 2005

Candida albicans, a polymorphic fungus, causes systemic and local infections. Recent reports show that the fungus is a main etiological agent for the arthritis. For trea tment, antifungal drugs and/or rheumatoid drugs are used, but resistance and side effects limit application of the drugs. In search of new sources for treatment of the fungal arthritis, we choose Egb 761 (extract of Ginkgo leaves 761), one of the most popular over-the-counter herbal medicines. The Egb 761 contains two major ingredients such as terpene and flavonoid. In the present study, we examined if the terpene portion of Egb 761 had anti-inflammatory activity against C.albicans-caused arthritis. The terpene was extracted with combination of methanol and water from the Egb 761, followed by gel-permeation chromatography. Presence of terpene was determined by the Salkowski colorimetric method and HPLC analysis. For an animal model of inflammation induction, mice were given an emulsion form of C.albicans cell wall mixed with Complete Freund's Adjuvant (CFA) by footpad-injection. Results showed that intraperitoneal administration of the water-soluble portion that contained terpene and flavonoid reduced the inflammation. Whereas the terpene had anti-inflammatory activity, flavonoid portion had no such activity, For determination of possible mechanism of the activity, the terpene seemed to be suppression of nitric oxide (NO) production from LPS-treated macrophages. Taken together the Ginkgo terpene may have anti-inflammatory effect against C.albicans-caused arthritis, possibly by blocking NO production.

• Journal of the Korean Wood Science and Technology
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• v.41 no.6
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• pp.559-565
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• 2013

This study evaluated the ability of white and brown rot fungi to decompose fire retardant-treated wood by measuring mass loss. Anti efficacy of FRT against sapstain and mold fungi was evaluated. Wood was treated with liquid sodium silicate and boric acid, ammonium borate, di-ammonium phosphate. Retardant treated wood was then subjected to fungal decay resistance tests performed according to KS standard method using a brown-rot fungus, Fomitopsis palustris and white rot fungus Trametes versicolor. Aspergillus niger, Penicillium funiculosum, Rhizopus nigricans, Aureobasidium pullulans, Tricoderma virede fungi were used anti-sapstain and mold test. Boron and phosphorus chemicals used in this study increased the resistance of fire retardant treated wood against both fungal attack. Anti mold and sapstain efficacy of the fire retardant treated wood was excellent but there were difference depend on mold. After the liquid sodium silicate treatment, the second chemical treatment process could lead chemical fixation into wood, which effects decay resistance.

• Mycobiology
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• v.51 no.4
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• pp.246-255
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• 2023

Genus Penicillium comprising the most important and extensively studied fungi has been well-known as a rich source of secondary metabolites. Our study aimed to analyze and investigate biological activities, including in vitro anti-cancer, anti-inflammatory and anti-diabetic properties, of metabolites from a marine-derived fungus belonging to P. levitum. The chemical compounds in the culture broth of P. levitum strain N33.2 were extracted with ethyl acetate. Followingly, chemical analysis of the extract leaded to the isolation of three ergostane-type steroid components, namely cerevisterol (1), ergosterol peroxide (2), and (3β,5α,22E)-ergosta-6,8(14),22-triene-3,5-diol (3). Among these, (3) was the most potent cytotoxic against human cancer cell lines Hep-G2, A549 and MCF-7 with IC₅₀ values of 2.89, 18.51, and 16.47 ㎍/mL, respectively, while the compound (1) showed no significant effect against tested cancer cells. Anti-inflammatory properties of purified compounds were evaluated based on NO-production in LPS-induced murine RAW264.7 macrophages. As a result, tested compounds performed diverse inhibitory effects on NO production by the macrophages, with the most significant inhibition rate of 81.37±1.35% at 25 ㎍/mL by the compound (2). Interestingly, compounds (2) and (3) exhibited inhibitory activities against pancreatic lipase and α-glucosidase enzymes in vitro assays. Our study brought out new data concerning the chemical properties and biological activities of isolated steroids from a P. levitum fungus.