Browse > Article
http://dx.doi.org/10.1080/12298093.2019.1687252

Optimization of Protoplast Preparation and Regeneration of a Medicinal Fungus Antrodia cinnamomea  

Wu, Jyun-De (Department of Life Science, National Dong Hwa University)
Chou, Jyh-Ching (Department of Natural Resources and Environmental Studies, National Dong Hwa University)
Publication Information
Mycobiology / v.47, no.4, 2019 , pp. 483-493 More about this Journal
Abstract
Antrodia cinnamomea is a unique medicinal fungus in Taiwan. It has been found rich in some pharmacologically active compounds for anti-cancer, hangover, and immune regulation etc. With the in-depth study of these components, it would be interesting and important to establish a molecular system for basic studies of A. cinnamomea. Thus, we would like to set up a foundation for this purpose by studying the A. cinnamomea protoplast preparation and regeneration. Firstly, we studied the optimization method of protoplast preparation of A. cinnamomea, and found various factors that may affect the yield during protoplast preparation, such as mycelial ages, pH values, and osmotic stabilizers. Secondly, in the regeneration of protoplasts, we explored the effects of various conditions on the regeneration of protoplasts, including different media and osmotic pressure. In addition, we found that citrate buffer with pH value around 3 dramatically increased the regeneration of protoplasts of A. cinnamomea, and provided a set of regeneration methodology for A. cinnamomea.
Keywords
Antrodia cinnamomea; citrate buffer; medicinal fungus; protoplast preparation; protoplast regeneration;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Lu MC, El-Shazly M, Wu TY, et al. Recent research and development of Antrodia cinnamomea. Pharmacol Ther. 2013;139(2):124-156.   DOI
2 Feng H, Sun Z, Li H, et al. Preparation, purification and regeneration optimizing research of protoplasts from Rhizoctonia solani. Afr J Microbiol Res. 2012;6:3222-3230.
3 Yu PW, Chang YC, Liou RF, et al. pks63787, a polyketide synthase gene responsible for the biosynthesis of benzenoids in the medicinal mushroom Antrodia cinnamomea. J Nat Prod. 2016;79(6):1485-1491.   DOI
4 Wubie A, Hu Y, Li W, et al. Factors analysis in protoplast isolation and regeneration from a Chalkbrood fungus, Ascosphaera apis. Int J Agric Biol. 2014;16:89-96.
5 Komoda K, Naito S, Ishikawa M. Replication of plant RNA virus genomes in a cell-free extract of evacuolated plant protoplasts. Proc Natl Acad Sci USA. 2004;101(7):1863-1867.   DOI
6 Fuchino K, Flardh K, Dyson P, et al. Cell-biological studies of osmotic shock response in Streptomyces spp. J Bacteriol. 2017;199:e00465-16.
7 Rehman L, Su X, Guo H, et al. Protoplast transformation as a potential platform for exploring gene function in Verticillium dahliae. BMC Biotechnol. 2016;16(1):57.   DOI
8 Geissenhoner A, Sievers N, Brock M, et al. Aspergillus nidulans DigA, a potential homolog of Saccharomyces cerevisiae Pep3 (Vps18), is required for nuclear migration, mitochondrial morphology and polarized growth. Mol Genet Genom. 2001;266(4):672-685.   DOI
9 Veses V, Richards A, Gow NAR. Vacuoles and fungal biology. Curr Opin Microbiol. 2008;11(6):503-510.   DOI
10 Lu ZM, He Z, Li HX, et al. Modified arthroconidial inoculation method for the efficient fermentation of Antrodia camphorata ATCC 200183. Biochem Eng J. 2014;87:41-49.   DOI
11 Hseu YC, Chang WC, Hseu YT, et al. Protection of oxidative damage by aqueous extract from Antrodia camphorata mycelia in normal human erythrocytes. Life Sci. 2002;71(4):469-482.   DOI
12 Song TY, Yen GC. Antioxidant properties of Antrodia camphorata in submerged culture. J Agric Food Chem. 2002;50(11):3322-3327.   DOI
13 Chen C-H, Yang S-W, Shen Y-C. New steroid acids from Antrodia cinnamomea, a fungal parasite of Cinnamomum micranthum. J Nat Prod. 1995;58(11):1655-1661.   DOI
14 Lee IH, Huang RL, Chen CT, et al. Antrodia camphorata polysaccharides exhibit anti-hepatitis B virus effects. FEMS Microbiol Lett. 2002;209(1):63-67.   DOI
15 Chang TT, Chou WN. Antrodia cinnamomum sp. nov. on Cinnamomum kanehirai in Taiwan. Mycol Res. 1995;99(6):756-758.   DOI
16 Chen ECF, Agrawal DC, Wang HY, et al. T-DNA activation-tagged mycelia producing fruiting bodies-specific triterpenoids in Antrodia cinnamomea - A medicinal fungus. Mycosphere. 2016;7(5):602-611.   DOI
17 Takeshita N. Coordinated process of polarized growth in filamentous fungi. Biosci Biotechnol Biochem. 2016;80(9):1693.   DOI
18 Geethangili M, Tzeng YM. Review of pharmacological effects of Antrodia camphorata and its bioactive compounds. Evidence-Based Compl Alt Med. 2011;2011:1.   DOI
19 Du YC, Wu TY, Chang FR, et al. Chemical profiling of the cytotoxic triterpenoid-concentrating fraction and characterization of ergostane stereoisomer ingredients from Antrodia camphorata. J Pharm Biomed Anal. 2012;58:182-192.   DOI
20 Lin JY, Wu TZ, Chou JC. In vitro induction of fruiting body in Antrodia cinnamomea - A medicinally important fungus. Botan Stud. 2006;47:267-272.
21 Chu YC, Yang RM, Chang TT, et al. Fructification of Antrodia cinnamomea was strain dependent in malt extract media and involved specific gene expression. J Agric Food Chem. 2010;58(1):257-261.   DOI
22 Eeckhaut T, Lakshmanan PS, Deryckere D, et al. Progress in plant protoplast research. Planta. 2013;238(6):991-1003.   DOI
23 Peberdy JF. Fungal protoplasts: isolation, reversion, and fusion. Annu Rev Microbiol. 1979;33(1):21-39.   DOI
24 Biot-Pelletier D, Martin VJJ. Evolutionary engineering by genome shuffling. Appl Microbiol Biotechnol. 2014;98(9):3877-3887.   DOI
25 Gow NAR, Latge JP, Munro CA. The fungal cell wall: structure, biosynthesis, and function. Microbiol Spectr. 2017;5(3). doi:10.1128/microbiolspec.FUNK-0035-2016
26 Gallmetzer M, Burgstaller W, Schinner F. An optimized method for the isolation of protoplasts from Penicillium simplicissimum to produce sealed plasma membrane vesicles. Mycologia. 1999;91(1):206-212.   DOI