• Title/Summary/Keyword: Anti-bacterial activity

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A Study on the Antibacteria Effect by Manufacturing Processes of Chrome-Tanned Garment Leather (의류용 크롬유혁의 제조공정에 따르는 항미생물 효과에 관한 연구)

  • 조승식;심미숙;김운배
    • Textile Coloration and Finishing
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    • v.5 no.1
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    • pp.33-39
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    • 1993
  • This study was to examine the anti-bacteria effect of chrome-tanned garment leathers by various processes to article from hide. Anti-bacterial test by halo test and shake flask method, and pH value measurment are carried out in this study. The results can be obtained as follows: 1. In the soaking process of chrome-tanned garment leathers, it needs more effective anti-bacterial treatment. 2. pH value of samples by various processes has affected to anti-bacteria effect. 3. Chrome used by the tanning and the neutralization process have been found to be the anti-bacteria activity. 4. The dyeing, the fatliquoring and the finishing process has required anti bacteria treatment.

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Anti-inflammatory and Anti-bacterial Active Ingredients Derived from the Extract of the Leaves of Hydrangea Petiolaris (등수국 잎 추출물 유래 항염 및 항균 활성 성분)

  • Jo, Seong Mi;Kim, Jung Eun;Lee, Nam Ho
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.46 no.3
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    • pp.207-218
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    • 2020
  • In this study, the anti-inflammatory and anti-bacterial activities of the extracts from the leaves of the Hydrangea petiolaris were identified, and the chemical structure was identified by separating the active ingredient. As the result of the anti-inflammatory activity experiment using RAW 264.7 cells, it was confirmed that the n-hexane (Hex) and ethyl acetate (EtOAc) fractions inhibited the production of nitric oxide (NO) and the expression of iNOS protein in a concentration-dependent manner without cytotoxicity. In addition, the n-Hex and EtOAc fractions reduced the production of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6). Upon the anti-bacterial tests using Staphylococcus epidermidis and Cutibacterium acnes, the extract, n-Hex, EtOAc and n-butanol (BuOH) fractions showed potent activities. In order to isolate the active constituents, the n-Hex and EtOAc fractions were further purified to afford four phytochemicals; phytol (1), corosolic acid (2), asiatic acid (3) and 1-O-p-coumaroyl-β-D-glucopyranoside (4). All of the compounds 1 - 4 were isolated for the first time from this plant. In addition, the contents of isolated compounds were determined by HPLC and the quantity of phytol (1) was 27.8 mg/g for the 70% EtOH extract. Based on the above research results, it is believed that it will be possible to develop a natural cosmetic material that has anti-inflammatory and anti-bacterial effects using the extract of H. petiolaris leaves.

Isolation of Antimicrobial Active Substances from Chinese Gall Nut (Schlechtendalia chinensis) against Watermelon Fruit Rot Pathogens (Acidovorax avenae subsp. Citrulli) (오배자(Schlechtendalia chinensis)로부터 수박 과실썩음병 병원균(Acidovorax avenae subsp. citrulli)에 대한 항균 활성물질 탐색)

  • Kim, Hyun-Woo;Choi, Yong-Hwa
    • Korean Journal of Organic Agriculture
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    • v.23 no.2
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    • pp.323-334
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    • 2015
  • This study was conducted to develop environment-friendly agricultural products with anti-microbial activity against Acidovorax avenae subsp. citrulli as a pathogen of bacterial fruit blotch in cucurbit. Schlechtendalia chinensis was extracted by MeOH and solvent fraction. The hexane fraction, which showed highest value of anti-microbial activity, was analyzed by GC-MS. Each mass spectra, corresponding to each peak of chromatogram, was compared to MS database of Wiley library. As a result, myristic acid, palmitic acid and 3-n-pentadecylphenol were identified as maine compounds showing antimicrobial activity against A. avenae subsp. citrulli. Bioassay using commercial myristic acid, palmitic acid and 3-n-pentadecylphenol to test for the anti-microbial activity conformed the anti-microbial activity of potential active compounds, and myristic acid and 3-n-pentadecylphenol showed strong activity. In conclusion, myristic acid and 3-n-pentadecylphenol identified from S. chinensis were anti-microbial chemicals.

Preparation of Multi Skin Care Gauze by Blending of Silk Fiber and Separated Cellulose from Waste Milk Pack (견사와 폐 우유팩으로부터 분리한 셀룰로오스가 함유된 복합 위생포 제작)

  • 여주홍;이광길;이용우;김종호
    • Journal of Sericultural and Entomological Science
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    • v.42 no.2
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    • pp.109-113
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    • 2000
  • The preparation of skin care gauze could be make to mixing separated cellulose from waste milk pack and degummed silk fibroin fiber. Also, its wound covering and anti-bacterial activity were investigated in order to find out the enhancement of their functionality. By the 30% silk fibroin fiber including skin care gauze, the anti-bacterial activity values of Staphylococcus strain are much 4 times higher than of 0∼10% silk fibroin fiber including skin care gauze. The average yield of cellulose from waste milk pack was obtained 50-60%, and their morphologies, physical properties, modulus and biodegradation ratios are studies, respectively.

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Study on the effects of herbal extracts on tooth whitening, antioxidant, nitric oxide synthesis and Streptoccocus mutans. (數種 韓藥材의 齒牙美白 效果에 관한 實驗的 硏究)

  • Jeong, Hyun-A;Rho, Seok-Sun;Lim, Seok-Yin
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.15 no.2
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    • pp.1-19
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    • 2002
  • Fifteen herbs used in oral medicine were extracted in ethanol and screened for tooth whitening effect, radical scavenging activity, inhibition of inducible nitric oxide synthase activity and anti-bacterial activity on Streptoccocus mutans. The results are as followed: 1. The tooth whitening effect of toothpaste containing 1 $\%$ ethanol extracts of 15 herbs was tested in brushing method using artificially stained HAT tablet and bovine tooth specimens. Toothpaste containing 1$\%$ of Vucia unijuga, Illicium verum, Angelica dahurica and Piper longum extracts showed tooth whitening effects on both HAT tablet and bovine tooth specimens. But the others did not show a considerable activity. 2. The antioxidant activity of ethanol extracts of 15 herbs was tested using the method of 1,1-diphenyl-2-picryl hydrazyl (DPPH) reactivity. Two ethanol extracts of Cimicifuga heracleifolia and Phyllostachys nigra var. henonis were found to be the most effective on radical scavenging activity. C. heracleifolia and P. nigra var. henonis extracts removed 86$\%$ and 81 $\%$ of DPPH radical at 0.01$\%$, compared with butylated hydroxy toluene as positive control. 3. The inhibition activity of ethanol extracts on nitrate production In RAW264.7 cell stimulated by lipopolysacchride was tested using the Griess reagent. But all extracts did not inhibit nitrate production. 4. The antibacterial activity on Streptoccocus mutans was tested by paper disk method. But no one extract showed any anti - bacterial activity.

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Anti-Bacterial Effects of Basil Oil on Streptococcus mutans and Porphyromonas gingivalis (Streptococcus mutans와 Porphyromonas gingivalis에 대한 Basil Oil의 항균효과)

  • Yoon, Hyunseo;Park, Chungmu
    • Journal of The Korean Society of Integrative Medicine
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    • v.6 no.3
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    • pp.131-139
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    • 2018
  • Purpose : The study objective was to assess the antibacterial activity of essential oil of basil against S. mutans and P. gingivalis and to explore its potential to prevent dental caries and peridontal disease. Method : Essential oil of basil, extracted using steam distillation, was diluted with triple distilled water and Tween 20 to generate samples at various concentration, that is 30%, 50%, and 70% (v/v). Strains of S. mutans and P. gingivalis were incubated in the medium under anaerobic condition. Broth microdilution susceptibility testing and plate incubation diffusion were utilized to determine the minimum inhibitory concentration (MIC) and to measure antibacterial activity, respectively. Result : An upsurge in antibacterial activity was seen to correlate with and increase in the concentration used for both bacterial strains, but was more significant with S. mutans. A statistically significant growth inhibition effect and reduction in the number of colonies was also observed with both strains dependent on the concentration used following 24 hours of incubation. Conclusion : Thus, the current study finding was that essential oil of basil was effective against dental caries and periodontal disease and could be used in dentifrice to help prevent oral disease.

A study on Antibacterial Activity of Samsinhwan Ethanol Extract against MRSA (삼신환(三神丸) 에탄올 추출물(SSH)의 MRSA에 대한 항균활성 연구)

  • Woo, Haksik;Lee, Yeoung Ju
    • Journal of Digital Convergence
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    • v.17 no.8
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    • pp.271-282
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    • 2019
  • The purpose of this study is to identify the antimicrobial effects of SSH(三神丸) and synerggy effects of the existing antibiotics Oxacillin and Ciprofloxacin on MRSA and to identify the mechanisms. In this case, the procedure and method for verifying anti-bacterial activity and active concentration of MARA by measuring the minimum inhibitory concentration (MIC) of the trichin is used to verify the potency and active concentration of MARA, to confirm the potency of the disease by treating antibiotics and trichine ethanol extract in parallel, and to confirm the anti-bacterial effect over time, when the trichine is activated as an anti-bacterial activity to MARA, and a compound. In addition, we hope that this research will not only serve as meaningful data for the development of new drugs to control MARA immunity, but also serve as an opportunity to further accelerate the research and development of antibiotics to overcome resistant strains.

Anti-acne and Anti-atopic Dermatitis Effect of Plant Extracts Including Eucommia ulmoides Oliv and Phellodendron amurense (두충나무, 황벽나무 등을 포함하는 수목추출물의 항여드름 및 항아토피 효과)

  • Kim, Gi Eun;Kim, Jin Hong;Hong, Seul Ki;Kim, Tagon;Kim, Donguk
    • Korean Chemical Engineering Research
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    • v.48 no.6
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    • pp.700-703
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    • 2010
  • In this study, plant extracts including Eucommia ulmoides Oliv. and Phellodendron amurense were studied to test possible application for cosmetics and skin related medicine. Anti-oxidation effect of plant extracts was measured by DPPH free radical scavenging activity and it was insignificant at low concentration, however, it was as good as vitamin C, excellent anti-oxidation agent, at $1000{\mu}g/ml$. Anti-bacterial effect was tested by disc diffusion method, and plant extracts showed mild anti-bacterial effect for normal skin flora, Staphylococcus epidermidis while it indicated strong anti-bacterial effect for acne inducing Propionibacterium acne. Therefore it had powerful potential for anti-acne material because of selectivity. Anti-atopic dermatitis effect was tested by hairless mouse and plant extracts recovered damaged skin to near normal condition after 14 days of treatment. IgE concentration in treated mouse was decreased 16% compared with control. From the research, plant extracts indicated strong anti-acne and anti-atopic dermatitis effect, and showed strong potential for cosmetics and skin related medicine.

Anti-Biofilm Activities of Manuka Honey against Escherichia coli O157:H7

  • Kim, Su-Yeon;Kang, Seok-Seong
    • Food Science of Animal Resources
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    • v.40 no.4
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    • pp.668-674
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    • 2020
  • Manuka honey (MH) has been shown anti-bacterial activity against several pathogenic bacteria. However, the inhibitory effect of MH on biofilm formation by Escherichia coli O157:H7 has not yet been examined. In this study, MH significantly reduced E. coli O157:H7 biofilm. Moreover, pre- and post-treatment with MH also significantly reduced E. coli O157:H7 biofilm. Cellular metabolic activities exhibited that the viability of E. coli O157:H7 biofilm cells was reduced in the presence of MH. Further, colony forming unit of MH-treated E. coli O157:H7 biofilm was significantly reduced by over 70%. Collectively, this study suggests the potential of anti-biofilm properties of MH which could be applied to control E. coli O157:H7.

Antibacterial Activity Evaluation of Radioisotope Lu-177 with a Modified Tube on Plate Core (중심부에 주입구가 존재하는 플레이트를 통한 방사성동위원소의 항균능력 측정)

  • Joh, Eun-Ha
    • Microbiology and Biotechnology Letters
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    • v.41 no.4
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    • pp.469-471
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    • 2013
  • In this study, we measured the anti-bacterial activity of radioisotope Lu-177 using a new laboratory instrument. The disk method used for the measurement of existing anti-bacterial antibiotics is drug diffusion into the medium. To measure the antimicrobial activity of a radioisotope, a new type of laboratory instrument is necessary to prevent the drug from spreading and the present invention was thus tested. In the medium, a space isolated separately for radioisotope injection was used to prevent the radioisotope from spreading and radioisotopes are actually injected by this system. We found that the antibacterial activity increased according to the radiation dose increases. It is expected that, through the present study, measuring the antibacterial activity of the other radioisotopes easily in the laboratory will be possible.