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http://dx.doi.org/10.5851/kosfa.2020.e42

Anti-Biofilm Activities of Manuka Honey against Escherichia coli O157:H7  

Kim, Su-Yeon (Department of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University-Seoul)
Kang, Seok-Seong (Department of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University-Seoul)
Publication Information
Food Science of Animal Resources / v.40, no.4, 2020 , pp. 668-674 More about this Journal
Abstract
Manuka honey (MH) has been shown anti-bacterial activity against several pathogenic bacteria. However, the inhibitory effect of MH on biofilm formation by Escherichia coli O157:H7 has not yet been examined. In this study, MH significantly reduced E. coli O157:H7 biofilm. Moreover, pre- and post-treatment with MH also significantly reduced E. coli O157:H7 biofilm. Cellular metabolic activities exhibited that the viability of E. coli O157:H7 biofilm cells was reduced in the presence of MH. Further, colony forming unit of MH-treated E. coli O157:H7 biofilm was significantly reduced by over 70%. Collectively, this study suggests the potential of anti-biofilm properties of MH which could be applied to control E. coli O157:H7.
Keywords
Manuka honey; Escherichia coli O157:H7; biofilm; anti-bacterial;
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1 Alvarez-Suarez JM, Giampieri F, Battino M. 2013. Honey as a source of dietary antioxidants: Structures, bioavailability and evidence of protective effects against human chronic diseases. Curr Med Chem 20:621-638.   DOI
2 Jefferson KK. 2004. What drives bacteria to produce a biofilm? FEMS Microbiol Lett 236:163-173.   DOI
3 Jenkins R, Cooper R. 2012. Improving antibiotic activity against wound pathogens with Manuka honey in vitro. PLOS ONE 7:e45600.   DOI
4 Kato Y, Umeda N, Maeda A, Matsumoto D, Kitamoto N, Kikuzaki H. 2012. Identification of a novel glycoside, leptosin, as a chemical marker of Manuka honey. J Agric Food Chem 60:3418-3423.   DOI
5 Khan SN, Khan S, Iqbal J, Khan R, Khan AU. 2017. Enhanced killing and antibiofilm activity of encapsulated cinnamaldehyde against Candida albicans. Front Microbiol 8:1641.   DOI
6 Kim H, Kang SS. 2019. Antifungal activities against Candida albicans, of cell-free supernatants obtained from probiotic Pediococcus acidilactici HW 01. Arch Oral Biol 99:113-119.   DOI
7 Kim NN, Kim WJ, Kang SS. 2019. Anti-biofilm effect of crude bacteriocin derived from Lactobacillus brevis DF01 on Escherichia coli and Salmonella Typhimurium. Food control 98:274-280.   DOI
8 Luis A, Silva F, Sousa S, Duarte AP, Domingues F. 2014. Antistaphylococcal and biofilm inhibitory activities of gallic, caffeic, and chlorogenic acids. Biofouling 30:69-79.   DOI
9 Mandal MD, Mandal S. 2011. Honey: Its medicinal property and antibacterial activity. Asian Pac J Trop Biomed 1:154-160.   DOI
10 Mohawk KL, Melton-Celsa AR, Zangari T, Carroll EE, O'Brien AD. 2010. Pathogenesis of Escherichia coli O157:H7 strain 86-24 following oral infection of balb/c mice with an intact commensal flora. Microb Pathog 48:131-142.   DOI
11 Nett JE, Cain MT, Crawford K, Andes DR. 2011. Optimizing a Candida biofilm microtiter plate model for measurement of antifungal susceptibility by tetrazolium salt assay. J Clin Microbiol 49:1426-1433.   DOI
12 Srey S, Jahid IK, Ha SD. 2013. Biofilm formation in food industries: A food safety concern. Food control 31:572-585.   DOI
13 Sherlock O, Dolan A, Athman R, Power A, Gethin G, Cowman S, Humphreys H. 2010. Comparison of the antimicrobial activity of Ulmo honey from Chile and Manuka honey against methicillin-resistant Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. BMC Complement Altern Med 10:47.   DOI
14 Sivaranjani M, Gowrishankar S, Kamaladevi A, Pandian SK, Balamurugan K, Ravi AV. 2016. Morin inhibits biofilm production and reduces the virulence of Listeria monocytogenes : An in vitro and in vivo approach. Int J Food Microbiol 237:73-82.   DOI
15 Sojka M, Valachova I, Bucekova M, Majtan J. 2016. Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm. J Med Microbiol 65:337-344.   DOI
16 Uhlich GA, Cooke PH, Solomon EB. 2006. Analyses of the red-dry-rough phenotype of an Escherichia coli O157:H7 strain and its role in biofilm formation and resistance to antibacterial agents. Appl Environ Microbiol 72:2564-2572.   DOI
17 Wu H, Lee B, Yang L, Wang H, Givskov M, Molin S, Hoiby N, Song Z. 2011. Effects of ginseng on Pseudomonas aeruginosa motility and biofilm formation. FEMS Immunol Med Microbiol 62:49-56.   DOI
18 Patel S, Cichello S. 2013. Manuka honey: An emerging natural food with medicinal use. Nat Prod Bioprospect 3:121-128.   DOI