• Journal of Ginseng Research
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• 제46권4호
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• pp.536-542
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• 2022

Background: In aged skin, reactive oxygen species (ROS) induces degradation of the extracellular matrix (ECM), leading to visible aging signs. Collagens in the ECM are cleaved by matrix metalloproteinases (MMPs). Syringaresinol (SYR), isolated from Panax ginseng berry, has various physiological activities, including anti-inflammatory action. However, the anti-aging effects of SYR via antioxidant and autophagy regulation have not been elucidated. Methods: The preventive effect of SYR on skin aging was investigated in human HaCaT keratinocytes in the presence of H₂O₂, and the keratinocyte cells were treated with SYR (0-200 ㎍/mL). mRNA and protein levels of MMP-2 and -9 were determined by real-time PCR and Western blotting, respectively. Radical scavenging activity was researched by 2,2 diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays. LC3B level was assessed by Western blotting and confocal microscopy. Results: SYR significantly reduced gene expression and protein levels of MMP-9 and -2 in both H₂O₂-treated and untreated HaCaT cells. SYR did not show cytotoxicity to HaCaT cells. SYR exhibited DPPH and ABTS radical scavenging activities with an EC₅₀ value of 10.77 and 10.35 ㎍/mL, respectively. SYR elevated total levels of endogenous and exogenous LC3B in H₂O₂-stimulated HaCaT cells. 3-Methyladenine (3-MA), an autophagy inhibitor, counteracted the inhibitory effect of SYR on MMP-2 expression. Conclusion: SYR showed antioxidant activity and up-regulated autophagy activity in H₂O₂-stimulated HaCaT cells, lowering the expression of MMP-2 and MMP-9 associated with skin aging. Our results suggest that SYR has potential value as a cosmetic additive for prevention of skin aging.

• 한방안이비인후피부과학회지
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• 제29권3호
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• pp.124-133
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• 2016

Objectives : Recently, the demands for the effective and safe depigmentating and anti-aging agents of the skin have increased due to the medical, pharmaceutical and cosmetic reasons. The aim of this study is to search new materials from the traditional herbal medicines which inhibit the aging process of skin in vitro.Methods : Human dermis cell (HS68) was used to test the effect of Rhus verniciflua Stokes (RVS). 80% ethanol or water extracts were screened for their inhibitory activities against elastase. Elastase inhibition effect was tested by microplate reader instrument. And MMP-1 suppression effect of RVS was tested by western blot. These cells were investigated the viability by MTS assay. And also the inhibition effect of tyrosinase by RVS was tested.Results : RVS (final concentrstion 1 ㎎/㎖ appeared over 30% of inhibition of elastase activity. So we are investigated anti wrinkle effects of Rhus verniciflua Stokes look through MMP-1 inhibition activity, also Extracts of RVS showed higher anti-tyrosinase activity than arbutin as final concentration 1 mg/ml. These results suggest that herbal medicines could be strong potential sources of inhibition of anti-aging and whitening effects for the skin.Colclusions : RVS was the best suppressor candidate of elastase activity among other control oriental drugs. It was found that RVS did not have toxicity to cells. We found proper concentration of RVS to treat in HS68 culture and investigated not only wrinkle care effect but also whitening effect of RVS.

• 한방안이비인후피부과학회지
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• 제34권4호
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• pp.1-11
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• 2021

Objectives : The aim of this study was to investigated the anti-aging and anti-wrinkles effects of the pinus koreaiensis seed(PKS) oil. Methods : The anti-oxidant effect was performed by beta-carotene bleaching assay and the intracellular proteome was analyzed expression of each 15 proteins by 2-D electrophoresis. And fatty acid was analysed by gas chromatography. Anti-wrinkle effect was analyzing human skin by the PRIMOS system. Results : Fatty acid analysis of PKS oil has shown oleic acid was 49.7% and linoleic acid was 34.1%. And the antioxidant effect was about 125% compared with alpha-tocoperol(0.1%) by beta carotene bleaching assay. In 2D PAGE analysis, fifteen protein changes in five mechanisms which was collagen synthesis pathway, MMPs, ECM-cell interaction, cytokine, antioxidant enzymes were analyzed. In case of anti-wrinkle effect was proved in vivo by analyzing human skin by the PRIMOS system. The analysis results of eye wrinkles for 4 weeks showed an improvement effect of over 6%. Conclusions : In this study, the amount of protein change in the five mechanism through the cell experiment and the skin anti wrinkle efficacy by the human in vivo test were investigated. As a result pinus koreaiensis seed oil by supercritical extraction could be used as a anti-aging and anti-wrinkle substance for the skin.

• 대한화장품학회지
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• 제24권3호
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• pp.17-23
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• 1998

Inner nutshell of Castanea mollisima BL (chestnut) has been used as an anti-aging and anti-wrinkle agent from the ancient time in east Asia. In order to develop new anti-aging and anti-wrinkle, ethanolic extract of inner nutshell of Castanea mollisima BL (Cor-285) was prepared and various biological activities were evaluated. Cor-285 showed potent antioxidant activity, Especially, Cor-285 possessed potent free radical scavenging activity in vitro (IC50:7.6 g/ml) compared to gallic acid (IC50:12.5 g/ml), Cor-285 showed the preventive effect against UV-induced cytotoxicity of fibroblast at concentration of 25-250 g/ml. When Cor-285 was evaluated for its anti-allergic activity, it effectively inhibited histamine release from mast cells induced by compound 48/80 (86% inhibition at 10 mg/ml). The inhibitory activity was stronger than that of glycyrrhiznate. Cor-285 also showed in vivo inhibition against delayed hypersensitivity as well as croton-oil induced ear edema in mice when topically applied These results strongly suggest that Cor-285 may reduce immunoregulatory 1 inflammatory skin trouble. From the attempts to isolate the constituents, citropten (simple coumarin) and ellagic acid, a well known radical scavenger, were isolated. In a clinical trial of twenty healthy volunteers with aged skin,6 weeks application of Cor-285 (3% cream) decreased wrinkle about 26% and increased moisturizing 20% on the skin. All of these results indicate that Cor-285 may be an effective anti-aging and anti-wrinkle agent.

• 대한화장품학회지
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• 제50권1호
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• pp.67-75
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• 2024

피부는 자외선, 감염 등과 같은 외부 요인에 의해 피부 노화가 진행된다. 이러한 요인들에 의해 피부의 섬유아세포는 단백질 분해효소인 matrix metalloproteinases (MMPs)를 분비한다. MMPs는 세포외기질에 위치하는 콜라겐의 분해를 유도하여 노화에 직접적인 영향을 미친다. 으름덩굴(Akebia quinata Decaisne) 줄기는 항산화, 항염증 효과가 있는 것으로 보고되었다. 하지만 으름덩굴 줄기 에탄올 추출물(AQSEE)의 항노화 효과에 대해서는 알려지지 않았다. 따라서 인간 섬유아세포에서 TNF-α로 유도된 MMP-1 억제 효과를 연구하였다. MTT asaay를 통해 AQSEE의 세포 생존율을 확인한 결과 400 ㎍/mL까지 독성을 나타내지 않았다. RT-qPCR과 ELISA를 통해 MMP-1 mRNA와 단백질 분비를 억제하는지 확인한 결과 100, 200, 400 ㎍/mL 농도에서 유의하게 감소하였다. 또한, western blotting을 통해 MAPKs 신호전달경로와 전사인자의 인산화가 감소하는지 확인하였다. 그 결과 p38, c-Jun, p65의 인산화가 모든 농도에서 유의하게 감소하였다. AQSEE의 radical 소거능을 확인하기 위해 DPPH, ABTS assay를 진행한 결과 모든 농도에서 유의하게 감소하였다. 본 연구 결과를 통해 MMP-1 억제 효과와 radical 소거능을 확인하였으며, 이는 항노화 물질로 사용될 수 있음을 시사한다.

• KSBB Journal
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• 제28권3호
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• pp.196-201
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• 2013

Silkworm-derived silk materials which contain 45 to 55% protein (18 amino acids, including 8 essential amino acids) have free radical-scavenging activities. In the present study, we investigated the whitening and anti-aging effects of silkworm gland under various conditions with B16F1 melanoma cells and human dermal fibroblasts. To evaluate the toxicity of soluble gland hydrolysate (SGH), mouse-derived B16F1 melanoma cells were treated with 0.2~5 mg/mL, cytotoxicity was not observed at all concentrations. The tyrosinase and elastase activities were significantly decreased as dosage levels of SGH increased. In addition, cell death was decreased by treated with SGH and antioxidative activity was the most effective in the SGH treatment. These findings suggest that SGH may be used as a potential functional biomaterial in having the skin whitening effect and anti-aging activity by inhibition of tyrosinase and elastase activities.

• 대한화장품학회지
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• 제36권4호
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• pp.295-301
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• 2010

본 연구에서는 천연 추출물을 이용하여 화장품 원료를 개발하고자 다양한 천연물을 대상으로 조사하였고 그중 광나무 추출물을 선별하여 주름 개선 원료로 사용하고자 하였다. 고성능액체크로마토그래피를 수행하여 주름 개선 효과로 잘 알려진 oleanolic acid 31.06 %와 ursolic acid 8.92 %를 확인하였다. 제조된 광나무 추출물은 피부 섬유아세포에서 procollagen type I과 hyaluronan synthase-3의 발현을 증가시키고 matrix metalloproteinase (MMP)-1, MMP-2, cyclooxygenase-2의 발현을 감소시킬 뿐 아니라 elastase의 활성을 억제하여 주름 개선 기능을 갖는 것을 확인하였다. 따라서 광나무 추출물은 주름개선 화장품 소재로 개발될 수 있는 잠재력이 있다.

• 한국식생활문화학회지
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• 제34권5호
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• pp.645-650
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• 2019

Skin plays important roles in protecting the internal organs from the chemical-biological risk factors and ultraviolet light. Exposure to the chemical and biological stimuli has a detrimental effect on skin's structure and physiological regulation. Therefore, much attention has been paid to natural products that show biological activities such as anti-oxidation, anti-aging and anti-bacterial activities. In this study, we investigated the skin-related biological activities of Oenothera lamarckiana aerial part extract. The extract contained 229.35 mg TAE (tannic acid equivalents)/g total polyphenolic compounds and the extract showed relative high antioxidant activity ($SC_{50}$ value: $8.52{\mu}g/mL$). The $IC_{50}$ value against tyrosinase and elastase were 307.94 and $181.51{\mu}g/mL$, respectively. This suggested that O. lamarckiana can be applied to whiten skin and slow the aging of skin. O. lamarckiana extract showed a growth inhibitory effect on Staphylococcu epidermidis (minimum inhibitory concentration: $250{\mu}g/mL$). Interestingly, O. lamarckiana extract showed no inhibitory effect on Pseudomonas aeruginosa on the paper disc assay. Yet the extract inhibited the growth of Pseudomonas aeruginosa on the broth dilution assay in a dose-dependent manners. Taken together, O. lamarckiana could have good potential for development as an additive in the cosmetic industry.

• Journal of Microbiology and Biotechnology
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• 제29권8호
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• pp.1212-1220
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• 2019

The study of metabolomics in natural products using the diverse analytical instruments including GC-MS, LC-MS, and NMR is useful for the exploration of physiological and biological effects and the investigation of drug discovery and health functional foods. Cordyceps militaris has been very attractive to natural medicine as a traditional Chinese medicine, due to its various bioactive properties including anti-cancer and anti-oxidant effects. In this study, we analyzed the metabolite profile in 50% ethanol extracts of C. militaris fruit bodies from three development periods (growth period, matured period, and aging period) using $^1H-NMR$, and identified 44 metabolites, which are classified as 16 amino acids, 10 organic acids, 5 carbohydrates, 3 nucleotide derivatives, and 10 other compounds. Among the three development periods of the C. militaris fruit body, the aging period showed significantly higher levels of metabolites including cordycepin, mannitol (cordycepic acid), and ${\beta}-glucan$. Interestingly, these bioactive metabolites are positively correlated with antitumor growth effect; the extract of the aging period showed significant inhibition of HepG2 hepatic cancer cell proliferation. These results showed that the aging period during the development of C. militaris fruit bodies was more highly enriched with bioactive metabolites that are associated with cancer cell growth inhibition.

• 대한화장품학회지
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• 제43권3호
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• pp.239-245
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• 2017

노화는 생리학적으로 비가역적으로 일어나는 과정으로 노화가 진행됨에 따라 단백질의 산화화학반응 등으로 노화징후가 축적된다. 활성산소와 당화된 최종 당화산물(advanced glycation endproducts, AGEs 최종 당화산물)은 생체 조직과 세포를 공격하여 노화를 촉진한다고 알려져 있다. 본 연구에서는 항산화 물질로 알려진 aminoguanidine을 양성 대조군으로 anti-glycation 효과를 확인하였으며, methoxy PEG-45 thioctate(LA-PEG)를 농도별로 처리하여 anti-glycation 효과를 평가하였다. 실험결과 LA-PEG는 매우 우수한 anti-glycation 효과로 최종 당화산물(AGEs) 생성억제 활성이 매우 우수하게 나타났으며, 항산화 효과와 밀접한 연관을 나타냈다. 또, 세포노화 지표물질인 senescence-associated ${\beta}$-galactosidase ($SA-{\beta}-gal$) 활성을 사람 섬유아세포(HDF)를 이용하여 확인한 결과, LA-PEG를 처리하였을 때 염색된 세포의 수가 감소하여 세포의 senescence를 억제하는 것을 확인할 수 있었다. 본 연구결과, LA-PEG의 anti-glycation 효과 및 산화로 인한 단백질 손상에 대한 보호 효과가 우수하게 나타났으며, 항노화 화장품에 적용 시 효과적으로 적용할 수 있을 것으로 사료된다.