• Title/Summary/Keyword: Anti-Wrinkles

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The Anti-Wrinkle and Whitening Effect of Extracts of Castanea crenata Inner Shell (밤부산물(율피)의 주름개선 및 미백 효과)

  • Jang, Min-Jung;Cheon, Soon-Ju;Kim, Hui-Yeong;Kwoen, Dae-Jun;Kim, Hak-Yoon;Kim, Sea-Hyun;Lee, Jin-Tae
    • Journal of Life Science
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    • v.21 no.5
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    • pp.734-738
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    • 2011
  • Aging in humans is inexorable and inescapable. The progressive decrease of physiological capacity and the reduction of the ability to cope with environmental stresses lead to increased susceptibility and vulnerability to human disease. Recently, in the cosmetic industry, many researchers have paid considerable attention to delaying or improving the symptoms of skin aging. Since the early 1990`s, there have been various challenges in developing cosmeceutical products which have strong anti-aging effects, and this has been an important issue in the cosmetic industry. Meanwhile, development of anti-aging cosmetics supported by biochemical activities in the skin has been researched. Castanea crenata inner Shell solvent extracts were investigated for anti-wrinkle and whitening effects, in order to apply it as a functional ingredient for cosmetic products. For anti-wrinkle effect, elastase inhibition activity of Castanea crenata inner shell acetone extract (CA) was 51.0% at a concentration of 100 ug/ml. The collagenase inhibition activity of CA and Castanea crenata inner shell ethanol extract (CE) was 96.4%, 94.3% at a concentration of 50 ug/ml. The tyrosinase inhibitory effect, which is related to skin-whitening, was 47.2% and 45.8% in CA and CE at a concentration of 500 ug/ml. All these results suggest that Castanea crenata inner shell can be effectively used as a cosmeceutical ingredient for the prevention of wrinkles.

Effect of Skin Wrinkle Reduction Using Emulsions with Microbiome Extracts Selected by 3D Human Skin Equivalents (3차원 배양 인공피부를 활용한 마이크로바이옴 추출물의 선정 및 이를 함유한 에멀젼 제형의 피부주름개선 임상 평가)

  • Jun Woo Lim;Yerim Kim;Jimin Jeong;Ji-Eun Kwon;Seong-Hyun Jo;Jindong Jang;Junsu Park;Yun-Gon Kim;Jae Hyun Jeong
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.49 no.1
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    • pp.47-58
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    • 2023
  • Recently, along with the remarkable increase in interest in microbiome cosmetics, the application of microbiome extracts in the complex efficacy as anti-aging, brightening etc. has become very important. In this study, Bifidobacterium bifidum (B. bifidum), which has excellent anti-wrinkle efficacy among the microbiome, was selected through an in vitro test using cells and 3D human skin equivalents. And the anti-wrinkle efficacy of cosmetics containing B. bifidum was evaluated by clinical test. Thereafter, the cytotoxicity, anti-oxidation, anti-inflammatory and anti-wrinkle efficacy of the of the bifida fermented filtrate were tested. An emulsion containing bifida fermented filtrate at a concentration of 5% (v/v) with no cytotoxicity and excellent efficacy was prepared with the placebo emulsion. The clinical test was conducted on a total of 21 women at 2 weeks comparing the bifida emulsion and placebo emulsion. Wrinkles around the eyes were instrumentally evaluated using Antera 3D. The wrinkle reduction rate of the Bifida emulsion group compared with the placebo emulsion group differed by 5.6%. Overall, the selection of microbiome using 3D human skin equivalents and the efficacy study of cosmetics with the microbiome extracts would be actively studied to the development of microbiome cosmetics and skin microbiome mechanisms.

Antioxidant, Anti-inflammation, and Anti-aging Effects of Ethyl Acetate Fraction from Cynanchum paniculatum Extracts (산해박 분획물의 항산화, 항염증 및 항노화 활성 효과 연구)

  • Jae Yong Seo;Go Eun Kim;Ji Soo Ryu;A Reum Jang;Su An Sim;Jung No Lee;Seunghee Bae
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.50 no.1
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    • pp.85-93
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    • 2024
  • In this study, we prepared fractions from Cynanchum paniculatum (C. paniculatum) with ethyl acetate and identified the structure and content of paeonol, an indicator substance. Fractions were obtained by sequential solvent fractions using n-hexane, methylene chloride, ethyl acetate, n-butanol, and distilled water, and showed the highest paeonol content in ethyl acetate (EtOAc). The structure of the ethyl acetate fraction (EA) was confirmed by nuclear magnetic resonance (NMR) and the content was determined by high performance liquid chromatography (HPLC) analysis. The antioxidant activity of EA was tested through its ability to scavenge 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and its ability to inhibit nitric oxide (NO) production in RAW 264.7 cells induced by lipopolysaccharide (LPS) was tested for its ability to inhibit NO production in a concentration-dependent manner. Furthermore, MMP-1 production induced by ultraviolet B (UVB) was decreased in a concentration-dependent manner by the EA, and the ability to produce procollagen type-I was increased in a concentration-dependent manner. In addition, as a result of conducting clinical trials using cosmetic formulations containing EA, the effect of improving wrinkles around the eyes was confirmed. Thus, it is expected that Cynanchum paniculatum can be used as a natural functional cosmetic material with antioxidant and anti-aging effects.

Whitening and Anti-Wrinkle Effects of Tremella Fuciformis Extracts (흰목이버섯 추출물의 미백 및 주름개선 효과)

  • Lee, Kwang Ho;Park, Hyun Soo;Yoon, Il Joo;Shin, Young Bong;Baik, Young Chan;Kooh, Dae Ho;Kim, Sung Kew;Jung, Ho Kyung;Sim, Mi Ok;Cho, Hyun Woo;Jung, Won Seok;Kim, Myoung Seok
    • Korean Journal of Medicinal Crop Science
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    • v.24 no.1
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    • pp.38-46
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    • 2016
  • Background : The white jelly mushroom (Tremella fuciformis), one of the most popular edible fungi, has medicinal properties. However, the effects of T. fuciformis in skin whitening or anti-wrinkle efficacy has not been defined to date. The aim of the present study was to investigate the effects of T. fuciformis extracts on whitening and anti-wrinkle efficacy in skin cells. Methods and Results :We prepared T. fuciformis extracts with water. The extracts ($80^{\circ}C$) contained 12.11 mg/g polyphenol and 8.54 mg/g flavonoid concentration. T. fuciformis extracts markedly decreased melanin contents and tyrosinase activity in ${\alpha}$-MSH-stimulated melanocytes (B16F10 cells). In addition, the mRNA expression of melanin formation factors, such as microphthalmia-associated transcription factor (MITF), tyrosinase-related protein-1 (TRP-1) and tyrosinase-related protein-2 (TRP-2) were significantly down-regulated in ${\alpha}$-MSH-stimulated melanocyte. Furthermore, T. fuciformis extracts increased the synthesis of type I procollagen and reduced mRNA expression of matrix metalloproteinase 1 (MMP-1) in the human dermal fibroblast (HDFn cells). These data indicated that T. fuciformis extracts induce repression of cellular melanogenesis and protect against wrinkles caused by UVB-stimulated damage. Conclusions : Thus T. fuciformis extracts could be a cosmetic candidate for skin whitening and anti-wrinkle effects.

Anti-wrinkle Effect of Morinda citrifolia (Noni) Extracts (노니 추출물의 주름개선 효과연구)

  • Lee, Jung-Noh;Kim, Sang-Woo;Yoo, Young-Kyoung;Lee, Ghang-Tai;Lee, Kun-Kook
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.32 no.4 s.59
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    • pp.227-231
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    • 2006
  • Wrinkle formation is mainly attributed to the environmental factors such as UV rays, air pollution, smoking and stress etc. Especially, UV rays induce premature skin aging which is characterized by deep wrinkle, leathery dryness etc. Recently, researches on the wrinkle formation and its prevention have been the main theme in cosmetics fields. We have studied the various plant extracts having anti-wrinkle effects and finally showed that Noni (Morinda citrifolia) extracts have the efficacy of promoting the type I collagen synthesis in normal human fibroblast, using PICP assay. We purified one active compound from Noni extracts and identified its structure. It was identified as 6,7-Dimethoxy-2H-1-Benzopyran-2-one; scopoletin by $^1H-NMR,\;^{13}C-NMR,$ IR, Mass analysis. Scopoletin increased collagen synthesis in a dose dependent manner (89.5% at $0.2{\mu}g/mL$). In order to verify the anti-aging effectiveness of the cream containing 3% noni extracsts, we performed the in vivo test with some female volunteers for 12 weeks. It reduced the signs of aging, especially face wrinkles. From these results, we conclude that the noni extracts could be used as an useful anti-wrinkle agent.

Anti-wrinkle Effect of Cosmetics Containing Duchesnea indica Extract (사매추출물을 함유하는 화장품의 주름 개선 효과)

  • Yang, Woong-Suk;Kim, Young-Min;Kim, Ee-Hwa;Seu, Young-Bae;Yang, Yoon-Jung;Kim, Hyun-Woo;Kang, Se Chan
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.36 no.4
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    • pp.281-288
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    • 2010
  • In this study, we investigated anti-oxidative effects of Duchesnea indica extracts by using Oxygen Radical Absorbance Capacity (ORAC). The extracts were prepared with 0 %, 30 %, 50 %, 70 % and 100 % aqueous ethanol respectively. The 30 % EtOH D. indica extract showed higher ORAC activity than the other extracts. Therefore, we performed in vitro studies on cytotoxicity of NIH-3T3 cells and MMP-8 collagenase inhibition using by the 30 % EtOH extract. The 30 % EtOH extract showed no cytotoxicity and significant inhibition on MMP-8 collagenase. And we performed clinical studies for the anti-wrinkle effect of the Di-Wrinkle Free Cream. The cream formula was prepared with 2 % arbutin and 1 % D. indica extract. Twenty one healthy women volunteers, ages of 35 and 50, applied the cream on their faces twice a day for 8 weeks. The skin was evaluated with PRIMOS (phaseshift rapid in vivo measuring of human skin) system and analyzed by the student's paired t-test. The wrinkles on the eye region were reduced by 13 % based on the PRIMOS system after 8 weeks. In the safety study of the Di-Wrinkle Free Cream, no symptoms were observed such as erythema, edema, scaling, itching, stinging, burning, tightness and prickling by visual observation and medical examination of volunteers for 8 weeks. Moreover, there was no noticeable skin disorder during experience period. These results suggested that D. indica extracts could be applied as cosmeceuticals effective for anti-wrinkle.

The Study on the Potential Anti-aging Properties of Prunella vulgaris Extract In Vitro and In Vivo (하고초 추출물의 항노화 효과에 관한 연구)

  • Hong, Eun-Suk;Ahn, Gi-Woong;Jo, Byoung-Kee
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.34 no.2
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    • pp.129-135
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    • 2008
  • In this study, the potential anti-aging properties of Prunella vulgaris extract were investigated. According to our results, Prunella vulgaris extract increased collagen synthesis(74.7% at 250 ${\mu}g/mL$) and decreased on MMP-1 synthesis(90.2% at 200 ${\mu}g/mL$) and elastase activity(43.7% at 2.0%). Furthermore, it also showed free radical scavenging activity(76.9% at 2.0%) and reduced $H_2O_2$-induced cytoxicity(49.9% at 2.0%). A double-blind clinical study to investigate the effect of Prunella vulgaris extract on the skin's surface was conducted with 22 healthy volunteers, aged 34 to 48 years. The volunteers applied a cream formula with 4.0% of Prunella vulgaris extract, or placebo cream, on each crow's feet twice a day for 12 weeks. Skin wrinkles were evaluated with the naked eye and instrumental image analysis of silicone replicas, followed by statistical analysis. Twelve weeks after application of cream formula with 4.0% of Prunella vulgaris extract, we found significant improvement of facial wrinkle. Moreover, silicone replica analysis confirmed notable improvement in average of R2 and R3 at 12 weeks(p<0.05). These results demonstrate that Prunella vulgaris provides a remarkable and significant tensor and anti-wrinkle effect on the skin, which could be of great use in anti-aging skin care products.

Consideration of preservation methods for plant genetic resources in natural monument - Focusing on preparation for becoming effective of Nagoya Protocol - (천연기념물 식물유전자원의 보존방안 고찰 - 나고야의정서 발효 대비 중심으로 -)

  • Kim, Jung A;Kim, Hyo Jeong;Shin, Jin Ho;Kim, Dae Yeol;Jo, Woon Yeon
    • Korean Journal of Agricultural Science
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    • v.41 no.3
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    • pp.193-203
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    • 2014
  • Natural Monument is a designated cultural property as part of the country. According to Article 2 of the Cultural Properties Protection Act, a national, ethnic and global heritage artificially or naturally formed, with a great historical, artistic, scientific and landscape significance is defined as a cultural heritage. Animals, plants, topography, geology, minerals, caves, biological products and special natural phenomena, having a great of historic, scenic and scientific value, are defined as the monument. According to Article 3 of Cultural Properties Protection Act, the conservation, management and utilization of National Heritage should be kept intact in its original form. So, Natural monuments are managing as retained its original form under the Basic Principles of current law. The highest population of coniferous tree in natural monument plant is ginkgo tree including 22 objects, followed by pines, junipers that order. And in case of broadleaf tree, there are zelkova trees, retusa fringe trees, pagoda trees, cork oaks, silver magnolias and etc. There are many of reported efficacy in available natural monument plants. The efficacy of plant species on pharmaceutical like anti-cancer, anti-diabetic, anti-obesity, antioxidant activity, neuroprotective, improves cholesterol, anti-inflammatory, liver protection and anti-bacterial efficacy, on cosmetics and beauty like the inhibiting formation of skin wrinkles, whitening effect, variety of materials and the efficacy of the proposed utilization of its various papers and etc have been widely reported. Before the Nagoya Protocol enters into force, the future role of the National Research Institute for Cultural Properties Administration of Cultural Heritage should be obtain a legal right to manage the social, cultural and national natural monument with emotional value to the plant genetic resource as a natural monument efficient ways to study and preserve traditional knowledge biological resources by securing a claim to the sovereignty of the material will be ready.

Supercritical Extraction of Oriental Herb : Anti-aging and Anti-wrinkle Effects (한방원료의 초임계 추출을 이용한 항노화 및 주름개선 효과)

  • Kim, In-Deok;Kwon, Ryun-Hee;Heo, Ye-Young;Jung, Hye-Jin;Kang, Hwan-Yul;Ha, Bae-Jin
    • KSBB Journal
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    • v.23 no.6
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    • pp.529-534
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    • 2008
  • The reactive oxygen species generated by ultraviolet rays causes various types of cutaneous damage, such as lipid peroxidation and denaturation of the extra-cellular matrix. The accumulation of such damage contributes to skin aging, especially the formation of wrinkles. This study was carried out to develop functional cosmatic by using Oriental herb supercritical extracts (OHSE) for prevention of skin. Effects of OHSE on anti-oxidation, collagenase inhibition and collagen synthesis in normal human fibroblast were investigated. OHSE showed antioxidative activity as high as vitamin C, trolox and DL-penicillamine. Also OHSE showed promotive effect on collagen synthesis and inhibitory effect on collagenase activity. From this results, we conclude that OHSE may have the potential to be conveniently used as an additive in cosmetics for prevention and improvement of skin aging.

A Study on Cosmetic Acupuncture Through Anatomy and Physiology Interpretation (해부생리학 해석을 통한 미용침의 연구)

  • Kim, Min-Sik
    • Korean Journal of Acupuncture
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    • v.30 no.3
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    • pp.171-177
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    • 2013
  • Objectives : The purpose of this study is to investigate the mechanism of Cosmetic Acupuncture through reinterpretation of anatomy and physiology. Methods : The causes of wrinkle increases and rapid aging of facial skin were studied and the theoretical system of Cosmetic Acupuncture treatment was analyzed through anatomy and physiology reinterpretation. Results and Conclusions : An increase in wrinkles and rapid aging of facial skin is caused by xerosis. Skin condition represents the condition of subcutaneous muscle. The reason why skin becomes easily dry is the heat produced by craniofacial part. Craniofacial part always generates lot of physiological fever because of the muscles. This physiological fever is produced from the muscles that are responsible for maintaining skull suture, controlling the movement of temporomandibular joint, maintaining head and neck posture. Controlling this fever is the crux of Cosmetic Acupuncture mechanism. These muscles correspond to Foot Taeyang meridian-muscle, Foot Soyang meridian-muscle and Foot Yangmyung meridian-muscle. Cosmetic Acupuncture is effective for preventing facial skin from aging and wrinkle increase by mechanical stimulus on facial muscles, and for controlling craniofacial part meridian-muscle system producing the heat.