• 제목/요약/키워드: Antheraea pernyi

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작잠(Antheraea pernyi) 아릴포린(Arylphorin) 유전자의 cDNA 클로닝 및 아릴포린 유전자의 발육시기 의존성 발현양상 (cDNA Cloning and Stage-Dependant Expression of Arylphorin Gene from Chinese Oak Silkworm, Antheraea pernyi)

  • 이상몽;황재삼;박남숙;김용균;김근기;손홍주;박현철;진병래
    • 생명과학회지
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    • 제20권8호
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    • pp.1193-1200
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    • 2010

  • 상수리 잎을 먹고 자라는 야생견사곤충의 일종인 작잠의 저장단백질인 아릴포린 유전자의 cDNA를 클로닝하고 발육경과에 따른 유전자발현의 양상을 조사 검토하였다. 작잠의 아릴포린 유전자의 cDNA는 2,112 bp의 ORF(open reading frame)를 포함하여 2,234 bp임을 밝혔다. 작잠 아릴포린 유전자의 cDNA염기서열로부터 아미노산 서열을 검토한 결과 방향족 아미노산인 페닐알라닌(phenylalanine)과 티로신(tyrosine)의 성분이 높은 아미노산 서열구조를 보였으며 ORF로부터 계산한 단백질의 분자량은 83,439 Da 이었다.작잠 아릴포린 저장단백질의 아미노산서열을 다른 곤충의 서열과 그 상동성을 비교 분석한 결과 세크로피아잠(H. cecropia)과는 78%, 담배나방의 알파단량체(M. sexta-$\alpha$ subunit)와는 71%, 담배나방의 베타단량체(M. sexta $\beta$-subunit)와는 62% 그리고 가잠(B. mori)과는 64%의 아미노산서열 상동성을 각각 보였다. 또, Northern blot analysis에서 유충 5령기의 중장, 중부 실샘, 후부 실샘에서는 아릴포린 유전자가 발현되지 않고 오로지 지방체 조직에서만 아릴포린 유전자가 발현되었음을 확인하였다. 아릴포린 유전자는 특히 종령인 5령 유충의 초기에 발현강도가 높았으며 중, 후기로 갈수록 그 강도가 감소하였다. 하지만 번데기시기에는 흔적 정도의 해당 mRNA가 검출되었으며, 이와 같이 검출된 mRNA는 불활성화된 것으로 추정된다. 이상의 결과에서 작잠 아릴포린 유전자의 cDNA가 클로닝되었으며, 이 유전자는 유충기특이적 발현 및 지방체 조직특이적 발현양상을 보인 점이 본 연구에서 확인되었다.

  • https://doi.org/10.5352/JLS.2010.20.8.1193 인용 PDF KSCI

A novel pattern recognition protein of the Chinese oak silkmoth, Antheraea pernyi, is involved in the pro-PO activating system

  • Wang, Xialu;Zhang, Jinghai;Chen, Ying;Ma, Youlei;Zou, Wenjun;Ding, Guoyuan;Li, Wei;Zhao, Mingyi;Wu, Chunfu;Zhang, Rong
    • BMB Reports
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    • 제46권7호
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    • pp.358-363
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    • 2013

  • In this paper, we firstly reported a C-type lectin cDNA clone of 1029 bps from the larvae of A. Pernyi (Ap-CTL) using PCR and RACE techniques. The full-length cDNA contains an open reading frame encoding 308 amino acid residues which has two different carbohydrate-recognition domains (CRDs) arranged in tandem. To investigate the biological activities in the innate immunity, recombinant Ap-CTL was expressed in E. coli with a 6-histidine at the amino-terminus (Ap-rCTL). Besides acted as a broad-spectrum recognition protein binding to a wide range of PAMPs and microorganisms, Ap-rCTL also had the ability to recognize and trigger the agglutination of bacteria and fungi. In the proPO activation assay, Ap-rCTL specifically restored the PO activity of hemolymph blocked by anti-Ap-rCTL antibody in the presence of different PAMPs or microorganisms. In summary, Ap-rCTL plays an important role in insect innate immunity as an pattern recognition protein.

  • https://doi.org/10.5483/BMBRep.2013.46.7.009 인용 PDF KSCI KPUBS HTML

Powdered Wound Dressing Materials Made from wild Silkworm Antheraea pernyi Silk Fibroin on Full-skin Thickness Burn Wounds on Rats

  • Kim, Min-Keun;Yoo, Ki-Yeon;Kwon, Kwang-Jun;Kim, Seong-Gon;Park, Young-Wook;Lee, Kwang-Gill;Jo, You-Young;Kweon, Hae-Yong
    • Maxillofacial Plastic and Reconstructive Surgery
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    • 제36권3호
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    • pp.111-115
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    • 2014

  • Purpose: This study evaluated powdered burn wound dressing materials from wild silkworm fibroin in an animal model. Methods: Fifteen rats were used in this experiment. Full-thickness $2{\times}2cm$ burn wounds were created on the back of rats under anesthesia. In the two experimental groups, the wounds were treated with two different dressing materials made from silkworm fibroin. In the Control Group, natural healing without any dressing material was set as control. The wound surface area was measured at five days, seven days and 14 days. Wound healing was evaluated by histologic analysis. Results: By gross observation, there were no infections or severe inflammations through 14 days post-injury. The differences among groups were statistically significant at seven days and 14 days, postoperatively (P<0.037 and 0.001, respectively). By post hoc test, the defect size was significantly smaller in experimental Group 1 compared with the Control Group and experimental Group 2 at seven days postoperatively (P=0.022 and 0.029, respectively). The difference between Group 1 and Group 2 was statistically significant at 14 days postoperatively (P<0.001). Group 1 and control also differed significantly (P=0.002). Group 1 showed a smaller residual scar than the Control Group and Group 2 at 14 days post-injury. Histologic analysis showed more re-epithelization in Groups 1 and 2 than in the Control Groups. Conclusion: Burn wound healing was accelerated with silk fibroin spun by wild silkworm Antheraea pernyi. There was no atypical inflammation with silk dressing materials. In conclusion, silk dressing materials can be used for treatment of burn wound.

  • https://doi.org/10.14402/jkamprs.2014.36.3.111 인용 PDF KSCI

The Antheraea pernyi Arylphorin That is Immunologically Cross-Reacted with the Storage Proteins of the Wild Silkmoths, Antheraea yamamai, Samia cynthia pryeri and Actias gnoma

  • Kim, Bo-Youn;Park, Gae-Jong;Park, Nam-Sook;Jin, Byung-Rae;Park, Sang-Bong;Kim, Hong-Ja;Seo, Sook-Jae;Lee, Sang-Mong
    • 한국잠사학회:학술대회논문집
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    • 한국잠사학회 2002년도 Join Meetings of Korean Society of Sericultural Science and Japanse Society of Sericultural Science
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    • pp.56-56
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    • 2002

  • No Abstract, See Full Text

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