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http://dx.doi.org/10.5483/BMBRep.2013.46.7.009

A novel pattern recognition protein of the Chinese oak silkmoth, Antheraea pernyi, is involved in the pro-PO activating system  

Wang, Xialu (School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University)
Zhang, Jinghai (School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University)
Chen, Ying (School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University)
Ma, Youlei (School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University)
Zou, Wenjun (School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University)
Ding, Guoyuan (School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University)
Li, Wei (School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University)
Zhao, Mingyi (School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University)
Wu, Chunfu (School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University)
Zhang, Rong (School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University)
Publication Information
BMB Reports / v.46, no.7, 2013 , pp. 358-363 More about this Journal
Abstract
In this paper, we firstly reported a C-type lectin cDNA clone of 1029 bps from the larvae of A. Pernyi (Ap-CTL) using PCR and RACE techniques. The full-length cDNA contains an open reading frame encoding 308 amino acid residues which has two different carbohydrate-recognition domains (CRDs) arranged in tandem. To investigate the biological activities in the innate immunity, recombinant Ap-CTL was expressed in E. coli with a 6-histidine at the amino-terminus (Ap-rCTL). Besides acted as a broad-spectrum recognition protein binding to a wide range of PAMPs and microorganisms, Ap-rCTL also had the ability to recognize and trigger the agglutination of bacteria and fungi. In the proPO activation assay, Ap-rCTL specifically restored the PO activity of hemolymph blocked by anti-Ap-rCTL antibody in the presence of different PAMPs or microorganisms. In summary, Ap-rCTL plays an important role in insect innate immunity as an pattern recognition protein.
Keywords
Antheraea pernyi; C-type lectin; Innate immunity; Pattern recognition protein; Pro-PO activation;
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