• Journal of fish pathology
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• v.27 no.1
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• pp.25-33
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• 2014

Hydrolase activities of excretory-secretory products (ESP) and somatic extracts (SE) from Anisakis simplex sensu stricto (s.s.) and Anisakis pegreffii larvae were investigated by using API ZYM kit. In esterase group, acid phosphatase showed high activity from both of A. simplex (s.s.) and A. pegreffii. Esterase (C4) showed activity only from SE and A. simplex (s.s.) showed higher activity than A. pegreffii. Alkaline phosphatase, acid phosphatase and naphthol-AS-BI-phosphohydrolase showed higher activity in 3rd stage larvae than in 4th stage larvae of both species. In aminopeptidase group, only leucine arylamidase showed remarkable activity in SE of both anisakid species, and A. simplex (s.s.) SE showed higher activity than A. pegreffii SE. In glycosidase group, N-acetyl-${\beta}$-glucosaminidase, ${\alpha}$-mannosidase, ${\alpha}$-fucosidase showed higher activity in A. simplex (s.s.) than A. pegreffii, and 4th larvae showed higher activity than 3rd larvae. These differences in hydrolase activity of anisakid nematodes larvae are thought to be due to different metabolism such as growth, moulting, digestion and feeding.

• 한국해양학회지
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• v.30 no.3
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• pp.197-202
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• 1995

A total of 272 specimens of Todarodes pacificus purchased during the period from July 1 to August 30, 1994 in the southern sea off Pusan were examined for their infection status with larval anisakids. Larvae in squids were encapsulated and appeared to remain active. Firty five larval anisakids sorted from T. pacificus (7.72% of infection rate) were classified based on morphological and morphometric observations as follows; Anisakis type I larvae (23 larvae, 51.0%: positive rate), Contracaecum type A (9, 20.0%), Contracaecum D (4, 9.0%), Anisakis II (3, 6.7%) and unknown type (6, 13.3%).

• Parasites, Hosts and Diseases
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• v.53 no.3
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• pp.349-353
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• 2015

Anisakis simplex sensu stricto (s.s.), Anisakis pegreffii, Anisakis berlandi (=A. simplex sp. C), and Anisakis typica are the 4 major species of Anisakis type I larvae. In the Republic of Korea (Korea), A. pegreffii, A. berlandi, and A. typica larvae in fish hosts has seldom been documented. In this study, molecular analysis was performed on Anisakis larvae from the sea eels (Astroconger myriaster), the major source of human anisakiasis in Korea, collected from Tongyeong City, a southern coastal area of Korea. All 20 sea eels examined were infected with Anisakis type I larvae (160 larvae; 8 per fish). Their species were analyzed using PCR-RFLP patterns and nucleotide sequences of internal transcribed spacers (ITS1, 5.8 subunit gene, and ITS2) and mitochondrial cytochrome c oxidase 2 (cox2). Most (86.8%; 112/129) of the Anisakis type I larvae were A. pegreffii, and 7.8% (10/129) were A. typica. The remaining 5.4% (7/129) was not identified. Thus, A. pegreffii is the major species of anisakid larvae in sea eels of the southern coast of Korea.

• Parasites, Hosts and Diseases
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• v.44 no.1 s.137
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• pp.81-86
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• 2006

Anisakiasis is a parasitic disease caused by ingestion of raw fish infected with anisakid larvae. Endoscopic changing patterns of submucosal lesions in chronic gastric anisakiasis have not been known yet. Here we report 4 cases of suspected gastric anisakiasis which were improved during follow-up periods without surgical treatment. The patients presented with abdominal pain, nausea and vomiting after consuming raw marine fish, and visited our gastroenterology outpatient department. Their endoscopic findings showed firm and yellowish submucosal masses accompanied with eccentric erosions. Histologic findings showed severe eosinophilic infiltrations. In blood tests, peripheral eosinophil counts and total IgE levels were elevated. We believed that all cases were caused by larval anisakid infections. The submucosal mass lesions disappeared during the follow-up periods of 2 to 4 mo.

• Parasites, Hosts and Diseases
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• v.33 no.3
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• pp.231-234
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• 1995

Anisakid larvae were found from the liver and stomach of the codfish (Notothenia negzecta) caught from the Antarctic Ocean, South Pole, where the Polar Research Center of The Korea Ocean Research and Development Institute is operating- Through observations by scanning electron (SEM) and light microscopy (LM), they were identified as the third stage larvae of Pseunotewnnovo necipiens, a potential agent of human codworm anisakiasis. They measured 25.0-28.5 mm in length, and 0.6-0.8 mm in width. The SEM revealed a prominent boring tooth, three lips, and excretory pore at the anterior end, and a small but prominent mucron at the posterior end. From whole mounts and cross sections of the larvae the ventriculus, intestinal cecum and a Renette cell were found characteristically at the same plane. Raw eating of the codfish caught from the Antarctic Ocean near the South Pole should be avoided so as to prevent human codworm anisakiasis in this area.

• Parasites, Hosts and Diseases
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• v.24 no.1
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• pp.1-11
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• 1986

Larval anisakids found in the yellow corvina (Pseudosciaena manchurica), a marine fish caught in the Yellow Sea, were classified by their morphological types. Total 1, 068 anisakid larvae were collected from 30 fish examined, with the average number per fish of 35.6. They were classified into Anisakis type I larvae of Berland (859 in number, 80.4%), Contracaecum type A of Koyama et at. (13, 1.2%), Contra caecum type C'(new type) (55, 5.1%), Contracaecum type D of Koyama et aZ. (18, 1.7%), Contracaecum type D' (new type) (77, 7.2%), Contracaecum type V of Yamaguti (3, 0.28%), Raphidascaris sp. of Koyama et aZ. (1, 0.09%) and unidentified (42, 3.9%). Contracaecum type C' and D' were considered new in the literature.

• Parasites, Hosts and Diseases
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• v.30 no.4
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• pp.263-268
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• 1992

A study was presented on the anisakid larvae in Astroconger myriaster which were caught at the Southern Sea and waled at Pusan area. The nematodes were morphologically classified into Anisakis sp. and Centracgecum sp. after fixation in 70% ethanol and clearing in lactophenol. Total of 1, 768 larval anisakids were collected from 259(67.8% positive rate) out of 382 examined A. myriaster. Total 642 larvae of Anisakis sp. were obtained from 94 (24.6%) and 1, 126 Ccntracaecum sp. were recovered from 165 (43.1%) fish. The average number of worms per infected fish was 4.6. The infection rate increased according to the length of fish, and all of the fish over 71 cm were found infected. The numbers (proportions) of recovered worms by the organs were 1, 440 (80.5%) in the omentuin, 166(9.4%) in the intestine, 107(6.0%) in the stomach, 32(1.8%) in the skin, 18(1.0%) in the muscle, 13(0.7%) in the liver, and 2(0.1%) in the head. The larvae in the muscle may infect the humans who are enjoying raw sliced meat of the fish.

• Journal of agricultural medicine and community health
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• v.16 no.1
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• pp.70-78
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• 1991

Antibody changes in experimental anisakiasis were observed by ELlSA and SDS-PAGE/EITB using various antigens : whole worm extract antigen(WWE), somatic antigen(SOM), excretory-secretory antign(ES), and hemoglobin antigen(HB) of Anisakis Type 1. The results obtained were as follows. l) Serum levels of IgG antibody by ELISA increased from 1st week of infection and reached their maximum titer at 5th week after infection, and decreased gradually thereafter. 2) The best result expressed as positive/negative ratio could be obtained when ES antigen was used. 3) Silver stained SDS-PAGE of each antigen showed at least 20 protein bands : In WWE, 286, 278, 262, 38, 18 Kd bands ; In SOM, 38 Kd band : In ES, 286, 65, 13 Kd bands ; In Hb, 61, 55, 38, 28, 26, 22, 20, 16, 15 Kd bands iepntibied as were major bands. 4) By EITB using WWE, Serum antibody recognized major protein with molecular weight of 86 Kd and 16 Kd. Using ES, 69, 59, 16 Kd bands were observed and using Hb, 28 Kd band was observed as specific band. In conclusion, excretory-secretory antigen(ES) of Anisakis larvae was most usable for ELISA.