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http://dx.doi.org/10.7847/jfp.2014.27.1.025

A comparison of the hydrolase activities of excretory-secretory products and somatic extracts from fish parasitic nematodes, Anisakis simplex sensu stricto and Anisakis pegreffii larvae  

Jeon, Chan-Hyeok (Department of Marine Bioscience, Gangneung-Wonju National University)
Wi, Seong (Department of Marine Bioscience, Gangneung-Wonju National University)
Kim, Jeong-Ho (Department of Marine Bioscience, Gangneung-Wonju National University)
Publication Information
Journal of fish pathology / v.27, no.1, 2014 , pp. 25-33 More about this Journal
Abstract
Hydrolase activities of excretory-secretory products (ESP) and somatic extracts (SE) from Anisakis simplex sensu stricto (s.s.) and Anisakis pegreffii larvae were investigated by using API ZYM kit. In esterase group, acid phosphatase showed high activity from both of A. simplex (s.s.) and A. pegreffii. Esterase (C4) showed activity only from SE and A. simplex (s.s.) showed higher activity than A. pegreffii. Alkaline phosphatase, acid phosphatase and naphthol-AS-BI-phosphohydrolase showed higher activity in 3rd stage larvae than in 4th stage larvae of both species. In aminopeptidase group, only leucine arylamidase showed remarkable activity in SE of both anisakid species, and A. simplex (s.s.) SE showed higher activity than A. pegreffii SE. In glycosidase group, N-acetyl-${\beta}$-glucosaminidase, ${\alpha}$-mannosidase, ${\alpha}$-fucosidase showed higher activity in A. simplex (s.s.) than A. pegreffii, and 4th larvae showed higher activity than 3rd larvae. These differences in hydrolase activity of anisakid nematodes larvae are thought to be due to different metabolism such as growth, moulting, digestion and feeding.
Keywords
Anisakis simplex sensu stricto; Anisakis pegreffii; Hydrolase activities; API ZYM;
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1 Wasko, A.P., Martins, C., Oliveira, C and Foresti, F.: Non-destructive genetic sampling in fish. An improved method for DNA extraction from fish fins and scales. Hereditas., 138: 161-165, 2003.   DOI   ScienceOn
2 Zhu, X.Q., Podolska, M., Liu, J.S., Yu, H.Q., Chen,H.H., Lin, Z.X., Luo, C.B., Song, H.Q. and Lin, R.Q.: Identification of anisakid nematodes with zoonoticpotential from Europe and China by single-strand conformation polymorphism analysis ofnuclear ribosomal DNA. Parasitol. Res., 101: 1703-1707, 2007.   DOI   ScienceOn
3 Chai, J.Y.: Fish-borne parasitic diseases. Hanyang. Med. Rev., 30: 223-231, 2010 (In Korean with english summary)   DOI
4 Arizono, N., Yamada, M, Tegoshi, T and Yoshikawa, M.: Anisakis simplex sensu stricto and Anisakis pegreffii: Biological characteristics and pathogenetic potential in Human anisakiasis. Foodborne. Pathog. Dis. 9: 517-521, 2012.   DOI
5 Bradford M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254, 1976.   DOI   ScienceOn
6 D'Amelio, S., Mathiopoulos, K.D., Santos, S.P., Pugachev, O.N., Webb, S.C., Picanco, M. and Paggi, L.: Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda; Ascaridoidea) defined by polymerase chain reactionbase restriction fragement length polymorphism. Int. J. Parasitol., 30: 223-226, 2000.   DOI   ScienceOn
7 D'Amelio, S., Busi, M., Ingrosso, S., Paggi, L and Giuffra, E.: Anisakis. In: Liu D.Y. (eds) Molecular detection of foodborne pathogens. CRC press, pp. 757-768, 2010.
8 Dziekonska-rynko J., Rokicki, J. and Jablonowski, J.: Activity of selected hydrolases in excretion-secretion products and homogenates from L3 and L4 larvae of Anisakis simplex (Nematoda: Anisakidae) parasitising herring. Acta. Ichthyol. Piscat., 33: 125-135, 2003.
9 Dziekonska-rynko J. and Rokicki, J.: Activity of selected hydrolases in excretion-secretion products and exracts of adult Contracaecum rudolphii. Wiad. Parasitol. 51: 227-231, 2005.
10 Dziekonska-rynko J.: The activity of selected hydrolases of adult female Ascaris suum, Goeze 1782. Helminthologia., 43: 59-63, 2006.   DOI
11 Lee D.L.: The histochemical localization of leucine aminopeptidase in Ascaris lumbricoides. Parasitology. 52: 533-538, 1962.   DOI
12 Hotez P.J., Cappello M., Hawdon J., Beckers C., Sakanari J.: Hyaluronidases of the gastrointestinal invasive nematodes Ancylostoma caninum and Anisakis simplex: possible functions in the pathogenesis of human zoonoses. J. Infect. Dis. 170: 918-926, 1994.   DOI   ScienceOn
13 Iglesias, L., Valero, A., Benitez, R. and Adroher F.J.: In vitro cultivation of Anisakis simplex: pepsin increases survival and moulting from fourth larval to adult stage. Parasitology., 123: 285-291, 2001.
14 Kim, W.S., Jeon, C.H., Kim, J.H. and Oh, M.J.: Current status of anisakid nematode larvae infection in marine fishes caught from the coastal area of Korea between 2010 and 2012. J. Fish. Pathol., 25: 189-197, 2012. (In Korean with english summary)   과학기술학회마을   DOI
15 Liew, S.M., Tay, S.T., Wongratanacheewin, S. and Puthucheary, S.D.: Enzymatic profiling of clinical and environmental isolates of Burkholderia pseudomallei. Trop. Biomed., 29: 160-168, 2012.
16 Nisbet, A. J. and Billingsley P.F: A comparative survey of the hydrolytic enzymes of ectoparasitic and free-living mites. Int. J. Parasitol. 30: 19-27, 2000.   DOI
17 Malagon, D., Benitez, R., Adroher F.J. and Diaz-Lopez, M.: Proteolytic activity in Hysterothylacium aduncum (Nematoda: Anisakidae), a fish gastrointestinal parasite of worldwide distribution. Vet. Parasitol., 183: 95-102, 2011.   DOI
18 Morris, S.R. and Sakanari, J.A.: Characterization of the serine protease and serine protease inhibitor from the tissue-penetrating nematode Anisakis simplex. J. Biol. Chem. 269: 27650-27656, 1994.
19 Nadler, S.A., D'Amelio, S., Dailey, M.D., Paggi, L., Siu, S. and Sakanari, J.A.: Molecular phylogenetics and diagnosis of Anisakis, Pseudoterranova, and Contracaecum from northern Pacific marine mamals. J. Parasitol., 91: 1413-1429, 2005.   DOI   ScienceOn
20 Oishi, K. and Hiraoki, M.: Food hygienic studies on Anisakis laravae-IV On the relation between the mortality and the penetration capacity of the larvae into an agar layer. Nippon Suisan Gakkaishi. 39: 1345-1348, 1973.   DOI
21 Rhodes M.B., Marsh C.L. and Ferguson D.L.: Studies in helminth enzymology. V. An aminopeptidase of Ascaris suum which hydrolyzes L-leucyl-$\beta$-naphthylamide. Exp. Parasitol. 19: 42-51, 1966.   DOI
22 Ondrovics, M., Silbermayr, K., Mitreva, M., Young, N.D., Razzazi-Fazeli, E., Gasser, R.B. and Joachim, A.: Proteomic analysis of Oesophagostomum dentatum (Nematoda) during larval transition, and the effects of hydrolase inhibitors on development. Plos. One. 8: e63955, 2013.   DOI
23 Setyobudi, E., Jeon, C.H., Choi, K.H., Lee, S.I. and Kim, J.H.: Molecular identification of anisakid nematodes third stage larvae isolated from common squid (Todarodes pacificus) in Korea. Ocean. Sci. J. 48: 197-205, 2013.   DOI
24 Quiazon, K.M.A., Yoshinaga, T., Ogawa, K. and Yukami, R.: Morphological differences between larvae and in vitro-cultured adults of Anisakis simplex (sensu stricto) and Anisakis pegreffii (Nematoda: Anisakidae). Paratiol. Int. 57: 483-489, 2008.
25 Rhoads M.L., Fetterer R.H. and Urban J.F.: Secretion of an aminopeptidase during transition of thrid- to fourth-stage larvae of Ascaris suum. J. Parasitol. 83: 780-784, 1997.   DOI
26 Sakanari, J.A., Staunton, C.E, Eakin, A.E. and Craik, C.S.: Serine proteases from nematode and protozoan parasites: Isolation of sequences homologs using generic molecular probes. Pro. Natl. Acad. Sci. 86: 4863-4867, 1989.   DOI
27 Smith, J.W. and Wootten, R.: Anisakis and Anisakiasis. Adv. parasitol. 16: 93-163, 1978.   DOI
28 Suzuki, J., Murata, R., Hosaka, M. and Araki, J.: Risk factors for human Anisakis infection and association between the geographic origins of Scomber japonicus and anisakid nematodes. Int. J. Food. Microbiol., 137: 88-93, 2010.   DOI   ScienceOn
29 Tabouret, G., Bret-Bennis, L, Dorchies, Ph and Jacquiet, Ph.: Serine protease activity in excretory-secretory products of Oestrus ovis (Diptera: Oestridae) larve. Vet. Parasitol., 114: 305-314, 2003.   DOI
30 Umehara, A., Kawakami Y., Araki, J. and Uchida, A.: Multiplex PCR for identification of Anisakis simplex sensu stricto, Anisakis pegreffii and the other anisakid nematodes. Parasitol. Int., 57: 49-53, 2008a.   DOI   ScienceOn
31 Ruitenberg E.J. and Loendersloot H.J.: Histochemical properties of the excretory organ of Anisakis sp. larvae. J. Parasitol. 56: 1149-1150, 1971.
32 Audicana, M.T. and Kennedy, M.W.: Anisakis simplex: from Obscure Infectious Worm to Inducer of Immune Hypersensitivity. Clin. Microbiol. Rev., 21: 360-379, 2008.   DOI   ScienceOn
33 Umehara, A., Kawakami, Y., Araki, J., Uchida, A. and Sugiyama, H.: Molecular analysis of Japanese Anisakis simplex worms. Southeast. Asian. J. Trop. Med. Public. Health. 36: 26-31, 2008b.