• 대한수의학회지
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• 제61권1호
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• pp.7.1-7.7
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• 2021

The levels of aflatoxin (AFT) and ochratoxin (OCT) were assessed at different seasons in raw materials, different feed manufacture processing stages, and animal feeds in feed mills in Korea implementing the hazard analysis and critical control point (HACCP) system. Two hundred samples were collected in all four seasons from five feed mills implementing the HACCP system and examined for AFT and OCT contents. The AFT and OCT levels were analysed by using HPLC method to provide information on raw material and product stage. The AFT content of raw ingredients in the spring season was highest in corn gluten (3.84 ppb) and lowest in corn (1.82 ppb) The AFT content of corn was highest in the winter season (2.17 ppb). The content of OCT in wheat was highest in the winter season. The amounts of AFT and OCT at processing stages were higher than in the raw materials or feed. In particular, AFT content was higher in the transfer stage (3.88 ppb) than in the mixing (2.86 ppb) or filling stages (3.45 ppb) in the summer season. The means of AFT and OCT level in laying hen feed was 3.41 ppb and 1.14 ppb for broiler feed, respectively. The means of AFT and OCT level in and broiler feeds was 3.44 ppb and 1.17 ppb for broiler feed, respectively.

• Asian-Australasian Journal of Animal Sciences
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• 제24권12호
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• pp.1757-1762
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• 2011

Aflatoxins are natural contaminants of poultry feeds and feed ingredients and cause liver damage, immunosuppression, reduction in performance and mortality in broilers. A number of studies have been carried out to study the effects of aflatoxin on feed conversion ratio in broilers. The results on feed conversion ratio of 10 research articles in broilers fed with aflatoxin from first day of age to six weeks of age were compiled and were subjected to meta-analysis. Chi-square test and $Tau^2$ (heterogeneity co-efficient) were applied to test for significance of heterogeneity of studies. To integrate results, fixed effect model by Inverse Variance method (IV method) was used when heterogeneity was insignificant and otherwise random effect model by DerSimonian and Laird Method (DL method) was used. The results of meta-analysis showed that the adverse effect of aflatoxin on feed conversion ratio at the end of first week was negligible, second week was medium and third to six weeks was very large.

• Journal of Animal Science and Technology
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• 제62권5호
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• pp.761-763
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• 2020

Here we report the complete genome sequence of Bacillus amyloliquefaciens ATC6, which produces acidic cellulase, isolated from pig feces. The genome is 4,062,817 bp in length and has a guanine-cytosine (GC) content of 46.27%. Among the predicted 3,913 protein-coding genes, two glucanase genes, which are involved in lichenan and cellulose degradation, were found. This genome analysis helps clarify the mechanism involved in cellulose biodegradation and support its application for efficient use of livestock feeds.

• Asian-Australasian Journal of Animal Sciences
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• 제12권2호
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• pp.215-219
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• 1999

This study aims to make detail examination of smallholder dairy farming systems in the Nongpho Dairy Cooperative. Forty-three dairy farms were selected from three geographical areas i.e. irrigated area, municipality area, and factory area. Within each area some number of sample farms were selected from each of the three levels of farm and animal crowdedness (very crowded, crowded, and not crowded farms). Detail data were collected during 1996 to 1997, they were socio-economic conditions of the sample farms and farmers, dairy production systems and management of resources (animals, bam, feeds, stocking rates, herd structure, animal body conditions, milk yield and milk quality, manure and farm wastes management, and other related items). Detail information useful for the improvement of farm production efficiency were discussed. It was very clear that much improvement of smallholder dairy production can be achieved if the recommendations given by this study were implemented.

• Asian-Australasian Journal of Animal Sciences
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• 제6권1호
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• pp.123-126
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• 1993

Hand dibbling in rows produced higher (p < 0.05) fresh and dry matter (DM), fodder yield, higher plant density and cob yield than broadcasted. However, the varying seed rates (40, 50 and 55 kg/ha) did not affect (p < 0.05) the fresh and DM yield and cob yield except for 30 kg/ha seed rate which produced the lowest (p < 0.01). However, the overall average fresh fodder yield and the fresh and DM yield of cob were 24.97, 10.04 and 6.90 t/ha respectively. The overall average plant height (cm), cob/plant (nos.) plant/ha, (nos.) cob/ha (nos.) and weight (g) of one cob were 178, 1.11, 664141, 70104 and 145 respectively for both the sowing methods and the four seed rates. Crude protein contents of maize plant and cob by-products (8.12% and 7.34% respectively) indicated promising cattle feeds along with human food.

• Animal Bioscience
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• 제36권2_spc호
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• pp.364-373
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• 2023

The gastrointestinal (GI) tract of ruminants contains diverse microbes that ferment various feeds ingested by animals to produce various fermentation products, such as volatile fatty acids. Fermentation products can affect animal performance, health, and well-being. Within the GI microbes, the ruminal microbes are highly diverse, greatly contribute to fermentation, and are the most important in ruminant nutrition. Although traditional cultivation methods provided knowledge of the metabolism of GI microbes, most of the GI microbes could not be cultured on standard culture media. By contrast, amplicon sequencing of 16S rRNA genes can be used to detect unculturable microbes. Using this approach, ruminant nutritionists and microbiologists have conducted a plethora of nutritional studies, many including dietary interventions, to improve fermentation efficiency and nutrient utilization, which has greatly expanded knowledge of the GI microbiota. This review addresses the GI content sampling method, 16S rRNA gene amplicon sequencing, and bioinformatics analysis and then discusses recent studies on the various factors, such as diet, breed, gender, animal performance, and heat stress, that influence the GI microbiota and thereby ruminant nutrition.

• Asian-Australasian Journal of Animal Sciences
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• 제18권3호
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• pp.340-344
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• 2005

In high-income Asian countries like Korea and Japan, per capita rice consumption has declined because of the change in consumer' favorite foods from rice to high-cost quality foods. This has forced farmers to reduce rice production. Although fodder rice could be another option to be cultured by farmers, available information concerning rice grain silage has been limited. In the present study, therefore, the difference in the quality of fodder rice silage prepared from either whole crop or grain at different ripening stages was compared. Various supplements were also added into whole crop and grain silages of fodder rice before ensiling, and thereafter, the palatability of prepared silages was determined by beef cattle. At ear emergence stage, the pH values for both grain and whole crop silages were approximately 4.5. In both grain and whole crop silages, the pH values were significantly increased by progressing ripening stages from milk-ripe stage to yellow-ripe stages, and the increase in pH value for grain silage was faster than that for whole crop silage. In the grain silage, the higher lactic acid (LA) content in grain silage seemed to be, the lower pH value was. Both in grain and whole crop silages, pH was significantly decreased by supplementation with LA bacteria. There were no significant differences in feed intake among any treatment groups.

• Asian-Australasian Journal of Animal Sciences
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• 제16권6호
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• pp.873-879
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• 2003

The study was conducted to improve the nutritive value of defatted rice polishing (DRP). DRP was treated with various concentrations of HCl, NaOH, $H_2O_2$ and Kemzyme-H $F^{(R)} and the effect on its chemical composition and nutritive value in broiler chicks was observed. The treatments levels of 0.4 N HCl, 0.2 N NaOH and 6% $H_2O_2$ were selected from many concentrations of HCl, NaOH and $H_2O_2$ tried earlier on DRP. The selection was made on the basis of release of nutrients from DRP. The Kemzyme-H $F^{(R)} was used at rate of 0.1% of DRP. The selected concentrations of HCl, NaOH, and $H_2O_2$ were then used for treatment of DRP that was used in biological experiments. Two hundred and forty, day-old Hubbard male broiler chicks (38-40 g) were randomly divided into 48 experimental units with five chicks each. Each chemically treated DRP was incorporated into broiler diets at 10, 20 or 30% levels replacing yellow corn from the control feed and thus sixteen experimental feeds were prepared. These feeds were randomly assigned to 48 experimental units such that there were three replicates of chicks on each diet. The results of the study suggest that DRP can be effectively used in broiler diets at 20% level. The best weight gain and feed conversion ratio were observed with diet containing 20% level of DRP treated with 6% $H_2O_2$. The diets containing 30% levels of treated DRP were uneconomical, as excess use of oil was required to compensate the energy needs of the birds.

• Asian-Australasian Journal of Animal Sciences
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• 제15권11호
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• pp.1659-1676
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• 2002

Ruminant animals develop a diverse and sophisticated microbial ecosystem for digesting fibrous feedstuffs. Plant cell walls are complex and their structures are not fully understood, but it is generally believed that the chemical properties of some plant cell wall compounds and the cross-linked three-dimensional matrix of polysaccharides, lignin and phenolic compounds limit digestion of cell wall polysaccharides by ruminal microbes. Three adaptive strategies have been identified in the ruminal ecosystem for degrading plant cell walls: production of the full slate of enzymes required to cleave the numerous bonds within cell walls; attachment and colonization of feed particles; and synergetic interactions among ruminal species. Nonetheless, digestion of fibrous feeds remains incomplete, and numerous research attempts have been made to increase this extent of digestion. Exogenous fibrolytic enzymes (EFE) have been used successfully in monogastric animal production for some time. The possibility of adapting EFE as feed additives for ruminants is under intensive study. To date, animal responses to EFE supplements have varied greatly due to differences in enzyme source, application method, and types of diets and livestock. Currently available information suggests delivery of EFE by applying them to feed offers the best chance to increase ruminal digestion. The general tendency of EFE to increase rate, but not extent, of fibre digestion indicates that the products currently on the market for ruminants may not be introducing novel enzyme activities into the rumen. Recent research suggests that cleavage of esterified linkages (e.g., acetylesterase, ferulic acid esterase) within the plant cell wall matrix may be the key to increasing the extent of cell wall digestion in the rumen. Thus, a crucial ingredient in an effective enzyme additive for ruminants may be an as yet undetermined esterase that may not be included, quantified or listed in the majority of available enzyme preparations. Identifying these pivotal enzyme(s) and using biotechnology to enhance their production is necessary for long term improvements in feed digestion using EFE. Pretreating fibrous feeds with alkali in addition to EFE also shows promise for improving the efficacy of enzyme supplements.

• 한국동물위생학회지
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• 제39권3호
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• pp.167-174
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• 2016

In this study, we analyzed for the changes of low organic compounds during 4 weeks incubation though inoculation of harmful microorganisms on commercial feed. Two percent of overnight cultures of Exiguobacterium acetylicum, Acinetobacter calcoaceticus, Aspergillus flavus and Fusarium graminearum were inoculated in feed, respectively. After adjusting moisture level to 50% for the promotion of feed spoilage, pH was decreased to 4.58~5.03 and microorganism was ranged to $6{\sim}10log_{10}CFU/g$. The compounds were compare between aflatoxin G1 producing feeds and aflatoxin G1 non-producing feeds. Aflatoxins G1 were detected by the immunoaffinity column clean-up method with HPLC-FLD, and were confirmed in samples incoulated by Aspergillus flavus and Acinetobacter calcoaceticus. Koiganal II, cyclohexanol and butadien-one were detected from samples (the non-sterilized inoculated feed) by Aspergillus flavus and Acinetobacter calcoaceticus. Respectively as aflatoxin G1 pre-detected substance, Koiganal II, cyclohexanol and butadien-one may be useful substance for the pre-detection of aflatoxin G1.