Browse > Article
http://dx.doi.org/10.7853/kjvs.2016.39.3.167

Analysis of low molecular organic compounds produced during the spoilage of dairy cattle compound feed  

Kim, Yong Tak (Department of Medicinal Biotechnology, Dong-A University)
Yi, Kwon Jung (Department of Animal Science and Technology, Konkuk University)
Kim, Gyeom-Heon (Department of Animal Science and Technology, Konkuk University)
Kim, Dong Woon (National Institute of Animal Science, RDA)
Kim, Soo Ki (Department of Animal Science and Technology, Konkuk University)
Moon, Hyung-In (Department of Medicinal Biotechnology, Dong-A University)
Publication Information
Korean Journal of Veterinary Service / v.39, no.3, 2016 , pp. 167-174 More about this Journal
Abstract
In this study, we analyzed for the changes of low organic compounds during 4 weeks incubation though inoculation of harmful microorganisms on commercial feed. Two percent of overnight cultures of Exiguobacterium acetylicum, Acinetobacter calcoaceticus, Aspergillus flavus and Fusarium graminearum were inoculated in feed, respectively. After adjusting moisture level to 50% for the promotion of feed spoilage, pH was decreased to 4.58~5.03 and microorganism was ranged to $6{\sim}10log_{10}CFU/g$. The compounds were compare between aflatoxin G1 producing feeds and aflatoxin G1 non-producing feeds. Aflatoxins G1 were detected by the immunoaffinity column clean-up method with HPLC-FLD, and were confirmed in samples incoulated by Aspergillus flavus and Acinetobacter calcoaceticus. Koiganal II, cyclohexanol and butadien-one were detected from samples (the non-sterilized inoculated feed) by Aspergillus flavus and Acinetobacter calcoaceticus. Respectively as aflatoxin G1 pre-detected substance, Koiganal II, cyclohexanol and butadien-one may be useful substance for the pre-detection of aflatoxin G1.
Keywords
Aflatoxin; Koiganal II; Cyclohexanol; Butadien-one; low organic compounds;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Kwonjung Yi, Yeon JS, Kim J, Kim C, Moon HI, Jeon CO, Lee SS, Kim DW, Kim SK. 2015. Microbiological and Chemical Changes of Complete Feed during Spoilage. Journal of Life Science 25: 1148-1155.   DOI
2 Pereira VL, Fernandes JO, Cunha SC. 2014. Mycotoxins in Cereals and Related Foodstuffs: A Review on Occurrence and Recent Methods of Analysis. Trends in Food Science & Technology 36: 96-136.   DOI
3 Warth B, Parich A, Atehnkeng J, Bandyopadhyay R, Schuhmacher R, Sulyok M, Krska R. 2012. Quantitation of Mycotoxins in Food and Feed from Burkina Faso and Mozambique using a Modern LC-MS/MS Multitoxin Method. Journal of Agricultural and Food Chemistry 60: 9352-9363.   DOI
4 Song YK, Jung BY, Choi JS, Moon OK, Lee SH, Chung DH, PAK SI, Park AC. 2010. Survey on aflatoxins in pig feeds in Korean pig farms. Korean Journal of Veterinary Public Health 34: 237-243.
5 Lee EY, Lim JS. 2011. Effects of the Feed and Probiotic Feeding on the Improvement of Hoggery Environment and the Productivity of Swine. Korean Journal of Microbiology and Biotechnology 39: 200-209.
6 Jang MR, Lee CH, Cho SH, Park JS, Kwon EY, Lee EJ, Kim SH, Kim DB. 2007. A Survey of Total Aflatoxins in Food Using High Performance Liquid Chromatography-Fluorescence Detector (HPLC-FLD) and Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). Korean journal of food science and technology 39: 488-493.
7 Reiter E, Zentek J, Razzazi E. 2009. Review on sample preparation strategies and methods used for the analysis of aflatoxins in food and feed. Molecular Nutrition & Food Research. 53: 508-524.   DOI
8 Lee SD, Kim YS, Yoon YT, Park AS, Shin Y, Kim HS, Kim YK, Choi BH. 2010. Monitoring of Aflatoxins in Herb Medicines, Korean J. Medicinal Crop Sci. 18(5): 338-344.
9 International Agency for Research on Cancer and World Health Organization. 1987. Monograph on the Evaluation of Carcinogenic Risks to Humans.
10 Bartov IN. Paster, Lisker N. 1982. The Nutritional Value of Moldy Grains for Broiler Chicks. Poultry Science 61: 2247-2254.   DOI
11 Kim KH, Cho ES, Kim KS, Kim JE, Seol KH, Park JC, Kim YH. 2015. Investigation on changes in pig farm productivity after ban of antibiotics growth promoter in commercial mixed feed. Journal of Agricultural Science 42: 223-229.
12 Kung L, Sheperd AC, Smagala AM, Endres KM, Bessett CA, Ranjit NK, Glancey JL. 1998. The Effect of Preservatives Based on Propionic Acid on the Fermentation and Aerobic Stability of Corn Silage and a Total Mixed Ration. Journal of Dairy Science 81: 1322-1330.   DOI