• Asian-Australasian Journal of Animal Sciences
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• v.16 no.3
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• pp.394-402
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• 2003

Individual and combined supplementation of phosphorus-adequate, wheat-based broiler diets with exogenous phytase and xylanase was evaluated in three experiments. The effects of the enzyme combination in lysine-eficient diets containing wheat and sorghum were more pronounced than those of the individual feed enzymes. The inclusion of phytase plus xylanase improved (p<0.05) weight gains (7.3%) and feed efficiency (7.0%) of broilers (7-28 days post-hatch) and apparent metabolisable energy (AME) by 0.76 MJ/kg DM. Phytase plus xylanase increased (p<0.05) the overall, apparent ileal digestibility of amino acids by 4.5% (0.781 to 0.816); this was greater than the responses to either phytase (3.6%; 0.781 to 0.809) or xylanase (0.7%; 0.781 to 0.784). Absolute increases in amino acid digestibility with the combination exceeded the sum of the individual increases generated by phytase and xylanase for alanine, aspartic acid, glutamic acid, glycine, histidine, isoleucine, phenylalanine, threonine, tyrosine and valine. These synergistic responses may have resulted from phytase and xylanase having complementary modes of action for enhancing amino acid digestibilities and/or facilitating substrate access. The two remaining experiments were almost identical except wheat used in Experiment 2 had a higher phytate concentration and a lower estimated AME content than wheat used in Experiment 3. Individually, phytase and xylanase were generally more effective in Experiment 2, which probably reflects the higher dietary substrate levels present. Phytase plus xylanase increased (p<0.05) gains (15.4%) and feed efficiency (7.0%) of broiler chicks from 4-24 days post-hatch in Experiment 2; whereas, in Experiment 3, the combination increased (p<0.05) growth to a lesser extent (5.6%) and had no effect on feed efficiency. This difference in performance responses appeared to be 'rotein driven'as the combination increased (p<0.05) nitrogen retention in Experiment 2 but not in Experiment 3; whereas phytase plus xylanase significantly increased AME in both experiments. In Experiments 2 and 3 the combined inclusion levels of phytase and xylanase were lower that the individual additions, which demonstrates the benefits of simultaneously including phytase and xylanase in wheat-based poultry diets.

• Sleep Medicine and Psychophysiology
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• v.1 no.2
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• pp.145-155
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• 1994

The neurophysiological study has been widely used in the search of the relation between brain and behavior. The basic techniques for the animal experiments of this kind such as stereotaxic techniques, lesioning methods, the methods of electrical stimulation and confirmation of histological location were simply reviewed. Nevertheless, the importance of complementary neurochemical, neuroanatomical and behavioral studies can not be neglected.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.4
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• pp.573-583
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• 2015

B-32 is one of a panel of monoclonal anti-idiotypic antibodies to growth hormone (GH) that we developed. To characterize and identify its potential role as a novel growth hormone receptor (GHR) agonist, we determined that B-32 behaved as a typical $Ab2{\beta}$ based on a series of enzyme-linked immunosorbent assay assays. The results of fluorescence-activated cell sorting, indirect immunofluorescence and competitive receptor binding assays demonstrated that B-32 specifically binds to the GHR expressed on target cells. Next, we examined the resulting signal transduction pathways triggered by this antibody in primary porcine hepatocytes. We found that B-32 can activate the GHR and Janus kinase (2)/signal transducers and activators of transcription (JAK2/STAT5) signalling pathways. The phosphorylation kinetics of JAK2/STAT5 induced by either GH or B-32 were analysed in dose-response and time course experiments. In addition, B32 could also stimulate porcine hepatocytes to secrete insulin-like growth factors-1. Our work indicates that a monoclonal anti-idiotypic antibody to GH (B-32) can serve as a GHR agonist or GH mimic and has application potential in domestic animal (pig) production.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.6
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• pp.643-646
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• 1996

Experiments were conducted to determine potassium (K) requirement of mule ducklings. One-day-old ducklings with equal number of both sexes were fed diets containing graded levels of K for three weeks. In experiment 1, corn-soybean meal diet (CP 18.7%, ME 2,890 kcal/kg, K 0.80%) was used. The addition of K (0.00, 0.10, 0.20, 0.30, 0.40%) to the diet by potassium carbonate resulted in a decrease in weight gain and feed efficiency with the group fed on basal diet having the best performance. This means that the existing K in the basal diet is adequate for the growth of ducklings. In experiment 2, low K corn-isolated soy protein diet (K, 0.19%) was used. The supplementation of K (0.00, 0.16, 0.32, 0.48, 0.64%) resulted in an increase in weight gain and feed efficiency with the control group having the lowest performance. The minimum K requirement was found to be 0.49% for both maximum growth and best feed efficiency as determined by using bent-stick model.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.4
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• pp.520-524
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• 1999

The present experiments were designed to examine whether exogenous DNA injected into the germinal crescent region (GCR) of early stage of developing embryos, which is considered to be the main place from which PGCs originate, can be transferred to recipient chicken embryos. In this experiment, Miw Z (DNA) dissolved in the transfection reagent (TR: Boehringer, Germany) was introduced into the GCR of donor embryos at stage 3-5 or 9-11, followed by continued incubation until the stage 13-15 of embryonic development. The PGCs collected from the embryonic blood vessels were examined for the incorporation of the injected DNA into the PGCs by the methods of X-gal staining and PCR analysis. As the results, the foreign DNA was successfully incorporated into the PGCS, leading to their transfer to the gonadal tissues. The present results, therefore, suggest that the early stage (3-5 or 9-11) of chicken embryonic development would be more successful than stage 13-15 in transferring exogenous genes to the recipient embryos, leading to the possibility of producing transgenic chicken medianting the PGCS.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.12
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• pp.1736-1743
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• 2011

This paper reports the peripheral and central effect of corticosterone on feed intake and hypothalamic corticotropinreleasing hormone (CRH) gene expression in chicks fed a high energy diet. Three experiments were conducted: corticosterone was supplemented to the feed (30 mg/kg diet), injected subcutaneously (s.c., 4 mg/kg body weight) or intracerebroventricularly (i.c.v., 4 ng). The results showed that dietary corticosterone significantly increased feed intake. The s.c. corticosterone administration increased feed intake within 1 to 3 h and at 1 to 5 h after the injection. The i.c.v. corticosterone administration increased feed intake within 1 h after the injection, but not at 1 to 3 h. Dietary supplementation and s.c. injection of corticosterone decreased the CRH gene expression in the hypothalamus. Therefore, peripheral corticosterone exerted a decreased effect on hypothalamic CRH mRNA levels, and corticosterone had a stimulating effect on feed intake in broiler chicks fed a high energy diet.

• Food Science of Animal Resources
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• v.29 no.5
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• pp.550-556
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• 2009

Two experiments were carried out to investigate the relationship of slaughter age and carcass traits of Hanwoo cattle. A total number of 11,284 heads of cattle (4,624 bulls, 6,120 steers, and 540 females) were used in the experiment (Exp.) 1. In the Exp. 2, 3,887 heads of steers slaughtered at age of 24-36 mon were used. After 24 h post-slaughter chilling, the carcasses were weighed and evaluated by an official grader of carcass traits according to the Korean carcass grading standard. Backfat thickness was the main variable in yield index and grade whereas marbling score was the main variable in carcass quality grade. Meat color was an important factor affecting carcass quality grade of bulls; fat color was an important factor affecting those of steers and females; and maturity was an important factor affecting that of females. After 24-mon-old, extension of slaughter age did not automatically increase the quantity and quality of meat. However, 29 mon of age can be regarded as the optimum time to slaughter steers to attain the highest take home money for farmers.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.10
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• pp.1430-1434
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• 2005

We conducted two experiments to assess the effect of transfer from housing to grazing on stress hormone secretion in cattle using urine samples. In a preliminary experiment, urine samples were collected following an adrenocorticotrophic hormone (ACTH) challenge, and cortisol levels in urine were compared with the levels in plasma. In a second experiment, urinary cortisol was measured before and after the start of a grazing season in 6 Japanese Shorthorn cows, all of which had experienced grazing before. In experiment 1, urinary cortisol showed a pattern of changes similar to that of plasma with a 0.5-h temporal lag time, and the peak levels were 4 to 10 times higher than the basal levels. In experiment 2, the urinary cortisol levels in cows did not change after the cows were let out to pasture, with no decreases in body weight. This study suggests that the transfer from housing to grazing did not affect physiological responses to cause high excretion of urinary cortisol in grazing-experienced cattle using a non-invasive sampling method.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.14 no.8
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• pp.3384-3398
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• 2020

In recent years, as the environment has become an important issue in dealing with food, energy, and urban development, diverse environment-related applications such as environmental monitoring and ecosystem management have emerged. In such applications, automatic classification of animals using video or sound is very useful in terms of cost and convenience. So far, many works have been done for animal sounds classification using artificial intelligence techniques such as a convolutional neural network. However, most of them have dealt only with the sound of a specific class of animals such as bird sounds or insect sounds. Due to this, they are not suitable for classifying various types of animal sounds. In this paper, we propose a sound classification scheme based on a multi-feature network for classifying sounds of multiple species of animals. To do that, we first collected multiple animal sound datasets and grouped them into classes. Then, we extracted their audio features by generating mixed records and used those features for training. To evaluate the effectiveness of our scheme, we constructed an animal sound classification model and performed various experiments. We report some of the results.

• Asian-Australasian Journal of Animal Sciences
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• v.7 no.2
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• pp.183-189
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• 1994

Two experiments were made. In experiment 1, four diets containing 0, 5, 15 and 25% washed seaweed meal were prepared to study the effects of incorporating seaweed meal instead of equivalent amounts of berseem leaf meal in fish feeds on growth performance and feed utilization of common carp. The results showed that average daily gain (ADG), specific growth rate (SGR%), dry matter (DM) and ether extract (EE) of the carcasses were decreased (p<0.05) with the increasing level of seaweed meal in the feeds. Inclusion of 5% seaweed meal in the diet gave the best feed conversion ratio (FCR) among all diets, however, protein productive value (PPV) and energy utilization (EU) were decreased (p<0.05) with increasing level of seaweed meal in the diet. In experiment 2, washed seaweed meal was either steam cooked or sprayed with NaOH (0.5% or 1% NaOH) and incorporated in the diets at the level of 25% instead of equivalent amount of berseem leaf meal. The results showed that steam cooked seaweed gave the best (p<0.05) growth performance, FCR and protein efficiency ratios, PER and PPV, for other treatments in descending order were NaOH treated seaweed, washed seaweed and unwashed seaweed.