• Journal of the Korean Applied Science and Technology
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• v.37 no.1
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• pp.66-75
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• 2020

Atopic dermatitis (AD) usually develops in patients with an individual or family history of allergic diseases, and is characterized by chronic relapsing inflammation seen specially in childhood, association with IgE hyperproduction and precipitation by environmental factors. and wished to examine closely effect that Polygonum multiflorum isolated PM-E and PM-70M orally adminstration used to atopy dermatitis disease patient get in atopy eruption control experimentally. Atopic dermatitis is a chronically relapsing inflammatory skin disease. Animal models induced by relevant allergens play a very important role in the elucidation of the disease. This study was investigated the anti-allergic effect of PM-E and PM-70M on BMAC induced atopic dermatitis in NC/Nga mice. We summerized as the follow. PM-E and PM-70M significantly reduced the skin number of total cell number, CD4⁺ and CD11b⁺/Gr-1 cell compared with positive control and decreased the invasion of CD4⁺ cell in dorsal skin tissue compared with positive control group by using immunohistochemical staining and chemokine such as eotaxin and CCR3 compared with positive control group. PM-E and PM-70M markedly suppressed invasion and edema of leukocytes and mast cell in dorsal skin. Taken together, these findings suggested that PM-E and PM-70M has an anti-allergic activity and this might be useful for the clinical application to treat allergic diseases such as atopic dermatitis.

• The Journal of Internal Korean Medicine
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• v.29 no.1
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• pp.90-103
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• 2008

Objective : This study wascarried out to understand the effect of Sopyung-tang (SPT) on blood glucose & antioxidant enzyme activities in streptozotocin-induced diabetic rats. Methods : SD rats were separated into three groups, each with 20 rats. Except the normal group, the other two groups were intra-peritoneally injected with streptozotocin 6mg/kg. The experimental group was treated with SPT extract 500mgkg/day for 4 weeks. The normal and control groups were treated with saline 500mg/kg/day for 4 weeks. Changes of plasma glucose level and body weight were observed. After4 weeks, liver and kidney weight, antioxidant enzyme activities, and survival rate were observed with histological changes on liver, kidney and pancreas. Results : In the experimental group, body weight and survival rate increased, while plasma glucose level decreased significantly. Liver and kidney weight, XOD activity decreased in the experimental group compared to the control. GSH-px and CAT activities andinsulin-immunoreactive granules in ${\beta}-cells$ increased significantly in the experimental group compared to the control. Conclusions : This study shows that SPT might be effective for treatment of diabetes and its complications, as well as reduction of oxidative stress.

• Korean Journal of Veterinary Service
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• v.37 no.3
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• pp.197-202
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• 2014

Dirofilaria immitis infection is one of the most important parasitic diseases in dogs and public health. It often elicits nodules in the pulmonary parenchyma and in the subcutaneous tissues of human. In this study, we investigated the prevalence of D. immitis infection among 754 dogs in Seoul area from April to December 2012. The infection rate of 754 dogs was 9.8% (74/754) by the antigen test (canine SNAP 4Dx). The infection rates of D. immitis in dogs at the age of < 2, 2~4, 5~7, 8~10, and > 10 years were 3.7% (7/189), 12.9% (30/233), 15.0% (19/127), 9.8% (18/184), and 0% (0/21), respectively. The infection rates in the female and male dogs were 9.7% (33/340) and 9.9% (41/414), respectively. The regional infection rates in northern and southern Han river region of Seoul were 10.7% (44/410) and 8.7% (30/344), respectively. In order to confirm D. immitis infection, we performed PCR on serum samples of 74 dogs which tested positive for D. immitis antigen by the antigen test and we detected a specific gene ITS-2 in 59 serum samples. In conclusion, this study suggests that treatment and preventive care on the D. immitis infection should be considered in dogs of Seoul area.

• The Journal of Korean Obstetrics and Gynecology
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• v.31 no.4
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• pp.128-146
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• 2018

Objectives: This study was performed to analyze the prescriptions of Korean herbal medicine which had been commonly used during postpartum period. Methods: We searched literature on postpartum cares with Korean Herbal medicine in 4 domestic search engines. After that, we conducted eligibility screening based on inclusion and exclusion criteria. Results: 1. We selected total 48 studies. There were 40 clinical studies (25 case reports and 15 case series) and 8 animal experimental studies. 2. Of the 40 clinical studies, other treatments were used together with herbal medicine : acupuncture (28), chuna manipulation (3), and pharmacopuncture (5). 3. Of the 25 case reports, the most common symptoms were musculoskeletal symptoms (8), followed by mental disorders including postpartum depression (5). Various prescriptions of herbal medicine were used depending on the diseases or symptoms. 4. In 15 case series, 665 subjects in 11 case series had taken Saenghwa-tang-gagam within 1~2 weeks after childbirth, and the average duration of Saenghwa-tang-gagam administration were $9{\pm}4.97$ days. 498 subjects in 9 studies were breast-feeding, and there were no side effects reported. 5. All experimental studies were about the recovery, the immunity and the breast-feeding associated puerperal characteristics. Also, 3 studies used Saenghwa-tang as an intervention. Conclusions: Saenghwa-tang can be commonly used for the purpose of prevention and care of the postpartum symptoms within the first 1~2 weeks after childbirth for 10 days. Also, various prescriptions of herbal medicine may be used depending on the diseases or symptoms, and can be conducted with other treatments like acupuncture, cupping, moxibustion, pharmacopuncture and chuna manipulation.

• Investigative Magnetic Resonance Imaging
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• v.23 no.4
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• pp.341-350
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• 2019

Purpose: To determine the MR parameters affecting India ink artifacts on opposed-phase chemical shift magnetic resonance (MR) imaging. Materials and Methods: The use of a female Sprague-Dawley rat was approved by our Institutional Animal Care and Use Committee. Using an iterative decomposition of water and fat with echo asymmetry and least-squares estimation (IDEAL) images, which is a modified Dixon method, axial opposed-phase images of the abdominal cavity were obtained with different MR parameters: series 1, different repetition times (TRs; 400, 2000, and 4000 ms); series 2, different echo times (TEs; 10, 50, and 100 ms); series 3, different field of views (FOVs; 6, 8, 16, and 24 cm); series 4, different echo train lengths (ETLs; 2, 4, and 8); series 5, different bandwidths (25, 50, and 85); and series 6, different slice thicknesses (1, 2, 4, 8, and 16 mm). Artifacts on opposed images obtained with different parameters were compared subjectively by two radiologists. For objective analysis, the thickness of the artifact was measured. Spearman's correlation between altered MR parameters and thicknesses of India ink artifact was obtained via objective analysis. Results: India ink artifact was increasingly apparent using shorter TE, larger FOV and ETL, and thicker slices upon subjective analysis. The objective analysis revealed a strong negative correlation between the thickness of the artifact and TE (r = -0.870, P < 0.01); however, strong positive correlations were found between FOV (r = 0.854, P < 0.01) and slice thickness (r = 0.971, P < 0.01). Conclusion: India ink artifact was thicker with shorter TE, larger FOV, and larger slice thickness.

• Clinical and Experimental Reproductive Medicine
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• v.30 no.4
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• pp.341-350
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• 2003

Objective: The purpose of this study was to identify dietary factors related to infertility in Korean women through a case-control study. Methods: The case group was composed of 236 women who had been diagnosed as infertility in hospital. The control group of 181 healthy women with children were recruited from local immunization centers. Socio-economic status, medical history, dietary intakes using food frequency questionnaire and stress were surveyed by interview. Anthropometric measurements were made and the causes of infertility were identified through medical records. Fasting blood samples were taken from subgroup of the subjects. Results: The mean age of infertile and control groups was 31.1 and 32.4 years, respectively and the difference was statistically significant. The mean Body Mass Index of infertile women was not significantly different from control women, however, Waist/ Hip Ratio and Triceps Skinfolds Thickness were significantly lower in infertile women than in control women. The dietary intake status was generally satisfactory in both groups. The intakes of energy, protein, fat, carbohydrate, retinol, vitamin B2 and niacin were lower in infertile women than in control women. The infertile women also showed lower intakes of animal foods. No differences were found between two groups in serum concentrations of albumin, hemoglobin, Fe, TIBC, total cholesterol, HDL-cholesterol, LDL-cholesterol, triglyceride, C3, IgA, IL-2, however, infertile women showed higher levels of Zn and IgG. The stress score was higher in infertile women. Conclusions: From the results of this study, dietary factors and nutritional status do not seem to be directly related to infertility. However, the intertile women have lower nutrient intake and lower body fat content than control women. Further researches are needed according to the causes of infertility for long term to establish the relationship between dietary factors and infertility.

• Journal of Pharmacopuncture
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• v.18 no.2
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• pp.42-50
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• 2015

Objectives: The aims of the study were to test the single- dose intravenous toxicity of Daebohwalryeok pharmacopuncture (DHRP) in Sprague-Dawley (SD) rats and to estimate the crude lethal dose. Methods: The experiments were conducted at Biotoxtech Co., a Good Laboratory Practice (GLP) laboratory, according to the GLP regulation and were approved by the Institutional Animal Care and Use Committee of Biotoxtech Co. (Approval no: 110156). The rats were divided into three groups: DHRP was injected into the rats in the two test groups at doses of 10 mL/kg and 20 mL/kg, respectively, and normal saline solution was injected into the rats in the control group. Single doses of DHRP were injected intravenously into 6 week old SD rats (5 male and 5 female rats per group). General symptoms were observed and weights were measured during the 14 day observation period after the injection. After the observation period, necropsies were done. Then, histopathological tests were performed. Weight data were analyzed with a one-way analysis of variance (ANOVA) by using statistical analysis system (SAS, version 9.2). Results: No deaths and no statistical significant weight changes were observed for either male or female SD rats in either the control or the test groups during the observation period. In addition, no treatment related general symptoms or necropsy abnormalities were observed. Histopathological results showed no DHRP related effects in the 20 mL/kg DHRP group for either male or female rats. Conclusion: Under the conditions of this study, the results from single-dose intravenous injections of DHRP showed that estimated lethal doses for both male and female rats were above 20 mL/kg.

• Journal of Dairy Science and Biotechnology
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• v.35 no.4
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• pp.232-243
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• 2017

Although several supplementary foods are available for pregnant and lactating women, there is a need for foods able to maintain a woman's beauty without any changes in body shape. In this study, four questionnaires about nutrition management, skin care, body shape, and the intent of purchasing beauty supplementary foods, were prepared for an online survey (http://pms8011.wixsite.com/project). A total of 95 answers from 293 women were classified into four groups, based on age, income, occupation, and skin type. The data were statistically analyzed using SPSS/WIN Program 22.0. The significances of the F-value analyzed by one-way ANOVA and the differences within the groups were verified by Scheffe's method. All groups were shown to have very high requirements for nutrition management and beauty standards. In the skin patterns, the groups of middle-aged women, house wife and the lower income showed high interests, with a high significance (P<0.01) in ages. With respect to body shape, significant interest was found within the groups of skin type (P<0.05) and age (P<0.001). With regard to supplementary foods, all groups showed a strong intention to purchase, but no significant differences were found. This study indicates the necessity to categorize supplementary foods by the intended target group, such as women planning to conceive, pregnant women, and lactating women.

• Reproductive and Developmental Biology
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• v.33 no.3
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• pp.139-146
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• 2009

This study tried to develop the system (device) that automatically notify a manager of condition just before and after farrowing to extend ubiquitous-based technology and to increase efficiency of delivery care and productivity by reducing human labor and time on standby when farrowing management is done in the difficult and hard working environment of farrowing such as night or holidays in field sand especially in pig industry. In this test, selected 10 gilts were executed timed artificial insemination and were set up each temperature sensor and load sensor to them 3 days before the estimated farrowing day and were observed the farrowing situation. This study was embodied the NESPOT-based (KT Corporation) monitoring system, the system to transmit data in real time by utilization of wireless LAN and the sensor module to apply the ubiquitous environment to them. And this study was observed the situation to automatically notify situations of 10 gilts that first bore just before and after farrowing. The result obtained the farrowing situations of them in real time by setup of the NESPOT-based monitoring system to check farrowing situation directly is as follow. The average time of the automatic notice about situation just before farrowing by the temperature sensor was 27.5 minutes before the beginning of farrowing (the expulsion time of a piglet). 6 of 8 pregnant gilts that first bore automatically were notified situations just before farrowing and the temperature sensors inserted into 2 ones before farrowing were omitted. (The automatic notice rate 75%) The average time of the automatic notice of situation just after farrowing by the load sensor was taken 46.5 minutes after the beginning of farrowing (the expulsion time of a first piglet). The average gestation period of 8 ones that first bore and were tested by the automatic notice of farrowing situation was 115.6 days. This result found that the automatic farrowing notice system by the temperature sensor is more efficient than the load sensor as the automatic farrowing alarm device and sanitary treatment and improvement of the omission rate were required.

• Journal of Veterinary Clinics
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• v.33 no.2
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• pp.131-134
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• 2016

A 4-year-old intact female Maltese was referred to the Animal Medical Center of Chonbuk National University with a history of consistent fecal incontinence over 4 months following sacculectomy surgery. We suspected that anal sacculectomy resulted in loss of the external anal sphincter. On physical examination, the external anal sphincter muscle on the left side was intact, while the external anal sphincter muscle on the right side could not be detected and exhibited severe laceration. To repair the defect, the left semitendinosus muscle was transposed around the anus. The left semitendinosus muscle was isolated and transected near the stifle, reflected dorsally and passed around the ventral rectum into the pararectal fossa. Care was taken to preserve the integrity of the vasculature and nerve supply. The muscle was secured dorsally with simple interrupted sutures to the levator ani and coccygeus muscles to simulate the external anal sphincter. Nine days after surgery, the dog was defecating normally with no evidence of incontinence. The use of semitendinosus muscle flaps is a good option for the treatment of fecal incontinence secondary to loss of sphincter muscle in dogs.