• 대한본초학회지
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• 제33권1호
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• pp.85-91
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• 2018

Objectives : This study was carried out to evaluate the quality of different drying methods and different roots(primary root and lateral root) of Angelica gigas Nakai. Methods : The experimental method was performed according to the Korea Pharmacopoea Eleventh edition (KP11). Loss on drying, ash, acid insoluble ash, ethanol extract, nodakenin and total decursin contents were tested to evaluate the quality of root tissue of Angelica gigas Nakai. In addition, the treatment of different root parts were prepared in two groups of washing dry process and natural dry process. Results : In comparison of dry processing methods, total contents of nodakenin and total decursin in the primary root and lateral root through washing dry process were ranged from 3.55 to 4.09% and from 5.18 to 6.13%, respectively. And also, those of roots from the natural dry process were from 4.36 to 6.22% and from 6.28 to 8.34%, respectively. In the washing dry process and natural dry process methods, 47.9% and 22.3% higher amount of nodakenin and total decursin were measured in lateral root compared to primary root. In common, lateral roots accumulated higher contents of nodakenin and total decursin compared to primary roots, and samples drying processed with natural dry process compared to washing dry process method contained higher amount of compounds. Conclusions : We sincerely hope that this study will be contributed to the standardization and quality control of Angelica Gigas Root.

• Preventive Nutrition and Food Science
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• 제14권3호
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• pp.179-187
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• 2009

Five kinds of coumarin compounds were successfully purified from Angelica gigas Nakai by using recyclingpreparative HPLC and identified as decursin (1), decursinol angelate (2), 7-demethylsuberosine (3), marmesin (4), and decursinol (5) by NMR analyses. None of the purified compounds in ethanol showed DPPH radical scavenging activity, while the A. gigas extract (AGNEX) displayed a significant level of activity. Interestingly, compounds 3 in phosphate buffered saline (PBS) showed good $ABTS^+$ radical scavenging activity ($IC_{50}=8.1{\mu}g$/mL) as did compounds 4 and 5. The anti-inflammatory activities of the purified compounds were evaluated and compared using the NO concentration assay and western blot analysis on LPS-stimulated RAW 264.7 cells. NO production was significantly suppressed by all the compounds in a dose-dependent manner among which compounds 1, 2, and 3 showed very good activities with $IC_{50}$ values of 7.4, 6.5, and $7.6{\mu}g$/mL, respectively. Treatment with compounds 1-5 effectively suppressed the expression levels of iNOS, IL-1$\beta$, and COX-2, which are responsible for promoting the inflammatory process. Thus, the ethanol extract and coumarin compounds of A. gigas Nakai hold promise for use as potential anti-inflammatory agents.

• 한국약용작물학회지
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• 제22권5호
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• pp.349-362
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• 2014

This study was designed to maximize extract yields of functional components in Angelica gigas Nakai by extrusion process. In addition, the optimizing condition of the extrusion process was established to increase the extraction of the functional components in A. gigas. The total polyphenol was increased by 40% compared to a control. And the optimizing condition was that the screw speed was 62.76 rpm, the amount of A. gigas powder was 34.79 kg/h, and the amount of water was 4.44 kg/h. The result of antioxidant activities of A. gigas extrusion, the inhibition of oxidization had the effect of 10.29 ~ 14.59% compared to a control. The content of decursin and decursinol angelate, which were index components, was 6.37%; it was increased by 16.64% compared to a control (5.31%) and showed a significantly difference (p < 0.05). And the optimizing condition was the screw speed 93.71 rpm, the amount of A. gigas powder was 28.67 kg/h, and the amount of water was 9.9 kg/h.

• 생약학회지
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• 제11권1호
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• pp.11-14
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• 1980

A Study of the effects of the root components of Angelica gigas Nakai (Umbelliferae) on voluntary activity in mice has confirmed that there are: 1) MeOH soluble extract, MeOH insoluble extract, decursin and decursinol were antagonized against the voluntary activity in mice pretreated with a dose of caffeine natrium benzoate 30mg/kg. 2) MeOH insoluble extract showed higher depressant effect than MeOH soluble extract. 3) The depressant activity of decursinol was significantly more highly effective than that of decursin.

• 대한한의학방제학회지
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• 제31권4호
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• pp.315-325
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• 2023

Objectives : Recently, research has been actively conducted on the efficacy of complexes based on oriental medicine prescriptions for improving immune activity and allergies. In this study, In this study, we aimed to examine the effect of Angelica gigas Nakai and Corni fructus extract (AC), medicinal herbs, among candidate drugs derived through preliminary experiments with various components of oriental medicine prescriptions for allergies, on allergies in RBL-2H3 cells. Methods : We evaluated the effect of the ethanol extract of Ulmus on the allergic inflammatory response in anti-DNP-IgE activated DNP-HSA in RBL-2H3 cells. Cell toxicity was determined by WST-1 assay and the markers of degranulation such as beta-hexosaminidase, histamine, TNF-α and IL-6 production of inflammatory mediators and FcεRI-mediated expression. Results : The results showed that treatment with AC extract (20, 40 and 80㎍/㎖) noncytotoxic levels and significantly inhibited the release of β-hexosaminidase, histamine and the production of TNF-α and IL-6 in RBL-2H3 by the antigen stimulation. Conclusions : These results indicate that AC extract exhibits anti-allergic activity through inhibition of degranulation and inhibition of inflammatory mediators and cytokine release. These findings suggest that AC extract may have potential as a prophylactic and therapeutic agent for the treatment of various allergic diseases.

• Korean Chemical Engineering Research
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• 제47권5호
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• pp.553-557
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• 2009

참당귀 뿌리 추출물에서 기능성 화장품소재를 개발하고자 하였다. 기기분석시험을 통하여 참당귀 뿌리 추출물에는 유효성분으로서 decursin과 decursinol angelate가 약 97%로 매우 고농도로 존재하였으며 그 비가 약 3:2로 나타났다. 화장품소재 시험으로는 항산화(DPPH free radical scavenging assay), 미백(Tyrosinase inhibition assay, Melanogenesis inhibition assay), 주름개선(Elastase inhibition assay), 자외선 흡수 및 안전성 시험(MTT assay)이 실시되었다. 참당귀 뿌리 추출물은 $15{\mu}g/ml$의 농도에서 tyrosine에 대한 tyrosinase 저해효과가 $45.2{\pm}3.9%$, melanin 생성억제 효과가 $24.2{\pm}12.0$로 미백효과가 우수하였다. 항산화효과는 $240{\mu}g/ml$의 추출물농도에서 DPPH free radical 소거율이 $40.9{\pm}9.1%$로 비교적 우수하였다. 주름개선 효과는 $100{\mu}g/ml$의 추출물농도에서 $12.7{\pm}6.8%$로 낮았으며, 자외선 흡수효과도 거의 없었다. 따라서 본 연구를 통하여 참당귀 뿌리 추출물은 화장품용 미백소재로서 가능성을 보여주었다.

• 한국식품과학회지
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• 제32권3호
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• pp.513-518
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• 2000

당귀의 정유물을 Nickerson 동시 중류 추출법으로 추출하여, 저장온도와 기간에 따른 정유량의 변화를 비교하였고, 저장 60일째의 각 시료의 휘발성 성분을 GC 및 GC-MS법으로 분석, 동정하였다. 당귀의 정유함량을 비교한 결과 저장 10일째는 저온저장$(1{\sim}5^{\circ}C)$과 상온저장한 당귀는 저장직전에 분석한 당귀의 정유량과 거의 차이가 없었으나 20일 이후에는 저온저장한 당귀 시료의 정유량이 상온저장한 당귀의 정유량보다 함량이 많았다. 상온저장한 시료는 저장기간이 길어짐에 따라 정유량의 중가가 완만하다가 60일에는 정유량이 증가하였다. 저온 저장한 시료는 저장기간 20일째는 정유량의 현저한 중가추세를 보였으나 60일까지는 유지 혹은 완만한 중가추세를 보였다. 당귀의 휘발성 성분을 분석한 결과 당귀의 주요 향기성분으로 극성 컬럼에서는 ${\beta}-elemene$ 등의 terpene hydrocarbon류, eudesmol 등의 terpene alcohol류, (-)-trans-myrtanyl acetate ester 등이 둥정되었다. 비극성 컬럼에서는 camphene 등의 terpene hydrocarbon류, ${\alpha}-eudesmol$ 둥의 terpene alcohol류, butylidene phthalide 등이 동정되었다. 특히 eudesmol류는 상온저장에서 전체 정유량의 45%, 저온저장에서 48%를 차지했다. 당귀의 휘발성 성분으로 극성과 비극성 컬럼에서 함량이 많은 eudesmol과 terpene alcohol류 등은 독특하고 상쾌한 당귀의 향에 기여하리라고 생각된다. 저장 방법 면에서 상온저장한 시료와 저온 저장한 시료를 비교해보면 당귀의 대부분의 향기성분의 함량은 저온저장한 시료에서 많았다. 그리고 eudesmol은 전체적으로 상온저장 시료와 저온저장 시료 양쪽에서 많았으나 저온저장한 시료 쪽의 함량이 더 많았다.

• 약학회지
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• 제44권2호
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• pp.119-127
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• 2000

This paper describes isolation and elucidation structure of the components from leaves and evaluate the radical scavenging activity on DPPH radical for antioxidant effect. Bioassay guided fractionation of MeOH extract afforded active EtOAc and BuOH factions. The most active EtOAc fraction was repeatedly chrormatographed over silica and Sephadex LH-20 to afford six flavonoid compounds. Studies on the antioxidant activity of these constituents showed that quercetin was the most active of these compounds. Luteolin and kaempherol are also active, These results suggested that the antioxidant activity of leaves of Angelica gigas may be due to flavonoid components. All the compounds were identified by spectroscopic methods and are the first report from leaves of Angelica gigas.

• 한국식품영양과학회지
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• 제27권3호
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• pp.399-405
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• 1998

This study was carried out to investigate the effects of herb extracts on lipid oxidation and free radical reaction in iron sources reacted with active oxygen species. The catalytic effects of active oxygen on lipid oxidation in oil emulsion tended to show more active in the order of OH, H2O2 and KO2. Herb extracts tended to show a little catalytic effect and active oxygen scavenging ability of herb extracts didn't show. But herb extracts played role as a strong chelating agents to bind iron if Fe2+ ion exist in oil emulsion. The contents of Fe2+ ion and total iron in Salvia miltiorrhiza Bge. and Angelica gigas Nakai were higher than those of Prunus persica Stockes and pinus strobus. The content of asocrbic acid in Pinus strobus showed the highest (26.97ppm) among several herb extracts. Electron donating abilities of Pinus strobus and Salvia miltiorrhiza Bge. were 79.54% and 77.11%, respectively, which were higher contents than those of Prunus persical Stokes and Angelica gigas Nakai. The SOD-like activity of Prunus persca Stokes showed 0.16 optical density (O.D), which means the most strong antioxidant activity among other herb extracts. The nitrite scavening effects tended to be different depending on pH. Pinus strobus and Angelica gigas Nakai showed 99.8% and 98.6% nitrite scavening effects at pH 1.2. And the effects were decreased as pH was increased. Especially, they didn't show the nitrite scavenging effect in pH 6.0. In conculsin, the Prinus strobus extract among herb extracts were the most effective antioxidant by evaluating several functional tests.

• 대한화장품학회지
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• 제44권1호
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• pp.59-66
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• 2018

Propionibacterium acnes (P. acnes)는 여드름의 주요 병원성균으로 염증성 질환에 관련된 미생물 중에 하나이다. 본 연구에서는 여드름 주요 유발 균주인 P. acnes에 대하여 70% Ethanol로 추출한 제주 자생식물 추출물의 항균활성을 disc diffusion test 실험을 이용하여 측정하였다. 실험 결과 61개의 제주 자생 식물 추출물 중에서 45개 추출물의 항균 활성이 검출되었으며, 16개의 추출물은 항균 활성이 검출되지 않았다. 그중에서 여우 구슬(Phyllanthus urinaria L.) - 줄기 잎 추출물이 $18.96{\pm}0.69mm$로 가장 높은 항균 활성을 나타내었고, 참당귀(Angelica gigas Nakai) - 뿌리, 돌외(Gynostemma pentaphyllum (Thunb.) Makino), 참당귀(Angelica gigas Nakai) - 잎 줄기, 산박하(Isodon inflexus (Thunb.) Kudo), 차나무(Camellia sinensis L.) - 꽃, 그리고 로즈마리(Rosmarinus officinalis L.) - 줄기 추출물 순으로 높은 항균 활성을 나타내었다. 이러한 결과는 제주 자생 식물 추출물이 여드름 치료 예방의 항균제 및 기능성 화장품 천연 원료로써의 이용 가능성을 제안한다.