• 대한본초학회지
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• 제22권4호
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• pp.95-100
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• 2007

Objectives : The purpose of this study is to investigate the delayed luminescence-biophoton emission from roots of Angelica gigas N., Angelica sinensis D., and Angelica acutiloba K These three species of Genus Angelica are now used as 'Danggui' in Traditional Korean Medicine. Methods : Randomly selected samples from roots of Angelica gigas N., Angelica sinensis D., and Angelica acutiloba K were radiated with 150 W metal halide lamp for 1 minute. After radiation, biophoton emissions of each sample were detected by electron multiplication-charge coupled device camera. The detected biophoton image was calculated with unit of counts per pixel. Results : The average and maximum biophoton emissions of delayed luminescence with electron multiplication ratio of ${\times}150$ and ${\times}250$were distinguished significantly between Angelica gigas N. and the other two species. Conclusions : These results suggest that biophoton imaging of roots of Angelica gigas N., Angelica sinensis D., and Angelica acutiloba K. could become the meaningful method for the study of differentiation between root of Angelica gigas N. and the other two species, Angelica sinensis D. and Angelica acutiloba K.

• Journal of Applied Biological Chemistry
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• 제50권4호
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• pp.264-269
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• 2007

Root extracts of Angelica gigas and A. acutiloba have been used traditionally for the treatment of gynecological diseases, as well as anemia, blood stasis, and inflammatory pain, as blood tonics in Oriental medicine. In the present study, we investigated the effects of A. gigas and A. acutiloba on inflammatory mediators in mouse macrophages and compared their activities. Many studies suggest that prostaglandin $E_2$ ($PGE_2$) biosynthesis and nitric oxide (NO) production play important roles in the processes of both inflammation and carcinogenesis. Ethanolic extracts from the roots of both species exhibited significant inhibitory effects on $PGE_2$ generation in lipopolysaccharide-stimulated RAW 264.7 cells. In particular, the extract from A. gigas was more effective than that from A. acutiloba. Although neither inhibited NO generation, the extract from A. acutiloba stimulated NO generation. Our results suggest that the roots of A. gigas might possess more anti-inflammatory and/or cancer chemopreventative activity than that of A. acutiloba due to the suppression of cyclooxygenase-2 (COX2)-mediated $PGE_2$ production. In addition, A. acutiloba might exert anti-tumor activity through an increase in macrophage-produced NO.

• Natural Product Sciences
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• 제17권3호
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• pp.202-205
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• 2011

The Angelica root has been used as a medicinal herb in many Asian countries including Korea, China, and Japan. Angelica gigas, A. sinensis, and A. acutiloba have been considered as Angelicae radix in Korean, Chinese, and Japanese Pharmacopoeia, respectively. Since the origins of Angelicae radix differ from country to country, there is a need to develop an efficient analytical method to identify the origin of the Angelica root. In order to obtain chemical fingerprints, three different Angelicae Radices were analyzed by direct analysis in real time mass spectrometry (DART-MS). Significantly different DART-MS spectra were observed from three different species of Angelicae Radix. Strong peaks of decursin or decusinol angelate, and its dimer were exclusively found from A. gigas. Ligustilide and linoleic acid were detected as the major component from A. acutiloba. The strongest ligustilide peak was observed from A. sinensis. DART-MS fingerprinting is a promising method for the rapid identification and/or quality control of Angelicae Radix.

• 한국약용작물학회지
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• 제13권2호
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• pp.121-125
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• 2005

An analysis of RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) was performed with three Angelica species (A. gigas Nakai, A. sinensis (Olive.) Diels and A. acutiloba Kitag) in an effort to distinguish between members of these three species. Two arbitrary primers (OPC02, OPD11) out of80 primers tested, produced 17 species-specific fragments among the three species. Eight fragments were specific for A. sinensis, four fragments specific for A. gigas, five specific for A. acutiloba. When primers OPC02 and OPD11 were used in the polymerase chain reaction, RAPD-PCR fragments that were specific for each of the three species were generated simultaneously. Primer OPC02 produced eight species-specific fragments: four were specific for A. sinensis, one for A. gigas, and three for A. acutiloba. Primer OPD11 produced nine speciesspecific fragments: four for A. sinensis, three for A. gigas, and two for A. acutiloba. The RAPD-PCR markers that were generated with these two primers should rapidly identify members of the three Angelica species. The consistency of the identifications made with these species-specific RAPD-PCR markers was demonstrated by the observation that each respective marker was generated from three accessions of each species, all with different origins. We also performed the RAPD-PCR analysis with the dried Angelica root samples that randomly collected from marketed and from the OPC02 primer, obtained a A. gigasspecific band and the band were cloned and sequenced.

• Toxicological Research
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• 제33권2호
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• pp.125-134
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• 2017

The effects that ultraviolet rays elicit on collagen synthesis and degradation are the most common causes of wrinkle formation and photo-aging in skin. The objectives of this study were to evaluate the effects of Angelica acutiloba root ethanol extract (AAEE) to promote collagen synthesis and inhibit collagen degradation in human dermal fibroblasts. By examining total polyphenol and flavonoid contents, electron donating ability, radical scavenging activity, and superoxide dismutase-like activity, we found that AAEE exhibited fairly good antioxidant activity. Treatment with AAEE significantly increased type I procollagen production by cultured fibroblasts, as well as reduced ultraviolet-induced matrix metalloproteinase-1 (MMP-1) expression and MMP-2 activity in a dose-dependent manner (p < 0.05). In addition, AAEE significantly increased TIMP-1 mRNA expression (p < 0.05), although without an associated dose-dependent increase in TIMP-1 protein expression. In summary, we suggest that AAEE may be a potentially effective agent for the prevention or alleviation of skin-wrinkle formation induced by ultraviolet rays.

• 한국약용작물학회지
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• 제12권1호
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• pp.43-46
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• 2004

당귀는 우리 나라는 참당귀 (Angelica gigas), 중국은 중국당귀 (A. sinensis) , 일본은 일당귀 (A.acutiloba)를 각각 같은 용도의 한약재로 사용하고 있다. 일당귀는 과거부터 우리나라에서도 일부 재배되고 있으며, 중국당귀는 ‘97년 이후 종자를 도입하여 재배하였으나 실패하여 농가의 피해가 발생한다. 이들 종의 생산성과 중국당귀의 국내 적응성을 수원 (해발 50 m), 진부 (해발 500 m), 태백 (해발 700 m) 3개지역에서 시험하였던 바 얻어진 결과를 요약하면 다음과 같다. 1. 참당귀, 일당귀는 3개 지역 모두 정상적으로 생육되고 수확이 되었다. 2. 중국당귀의 생존율은 테백 70.3%, 진부 45.2%, 수원 4%로 수원에서는 7월 이후 고온기에 고사되었다. 3. 중국당귀의 10a당 수량은 태백 160 kg, 진부 127 kg, 수원 8 kg으로 태백, 진부에서는 수확이 가능하였으나 수량이 극히 낮았으며, 수원에서는 생산이 불가능하였다. 4. 중국당귀는 해발 500 m 이하에서는 생존율이 극히 낮으며, 해발 700 m 이상의 높은 지역에서는 생존은 가능하나 생산성이 낮아 국내생산은 어려운 것으로 나타났다.

• 한국약용작물학회지
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• 제11권5호
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• pp.352-357
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• 2003

참당귀의 전초 (꽃봉우리, 줄기, 잎)와 뿌리의 정유성분을 분석한 결과 전초의 정유 함량은 0.063%로 뿌리의 0.389%에 비해서 아주 적은 함량을 나타내었다. 주요 성분에 있어서 전초와 뿌리의 정성적 차이는 없었으나, 함량에 있어서는 큰 차이를 보였다. 주요 휘발성 성분은 nonane, ${\alpha}$-pinene, limonene + ${\beta}-phellandrene$이였으며, 이 중 nonane과 ${\alpha}-pinene$의 경우 전초에서는 각각 7.5%와 14.6%이었으나, 뿌리에서는 각각 24.5%와 31.7%로 많은 양을 나타내었다. 이 외에 ${\gamma}-terpinene$, germacrene-d, (E,E)-${\alpha}-farnesene$, ${\beta}- eudesmol$의 경우 전초에서는 5%에서 8% 사이로 나타났으나, 뿌리 에서는 1% 안팎의 적은 함량을 보였다. 참당귀와 일당귀를 분석한 결과 줄기와 잎에서 각각 0.068%, 0.127%와 0.153%, 0.243%로 정유의 함량은 일당귀가 더 높았고, 부위별로는 잎이 줄기의 약 2배 정도 높았다. 참당귀 줄기와 잎의 성분으로는 각각 18개, 32개로 잎에서 더 다양한 향기성분이 분포되어 있었고, 주요 휘발성 성분으로는 ${\alpha}-pinene$, myrcene, limonene, germacrene-d, eudesmol and butylphthalide로 잎에서 germacrene-d가 높았고, butylphthalide가 낮은 차이를 보였다. 일당귀줄기와 잎의 휘발성 정유 성분으로는 각각 21개, 32개로 참당귀와 같이 잎에서 더 다양했고, 성분은 ${\gamma}-terpinene$과 butylphthalide로 butylphthalide가 70%에 육박하는 주성분을 차지했다. 그러므로 Angelica gigas와 Angelica acutiloba는 관능적으로 확연히 다른 차이를 느낄 수 있지만, 분석결과로도 전혀 다른 향기성분임을 알수 있었다.

• 생약학회지
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• 제36권2호통권141호
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• pp.141-144
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• 2005

Chinese crude drug 'Dang Gui' has been used mainly as a tonifying the blood, promoting blood circulation, relieving pain and moistening the bowels. To clarify the botanical origin of 'Dang Gui' from Korea, the anatomical characteristics of Angelica gigas, Angelica sinensis and Angelica acutiloba were studied. As a result, it was clarified that 'Dang Gui' from Korea was the root of Angelica gigas.

• Plant Resources
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• 제8권1호
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• pp.71-74
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• 2005

Activated carbon(AC) can be utilized as a soil conditioner in agricultural crop areas. This study was conducted to investigate the effect of AC on Leaf and Stem Production of Angelica acutiloba as affected by different amounts of AC. The results obtained are summarized as follows. Growth characteristics including plant height and leaf length were the highest when activated carbon added with 10, suggesting that optimum amount of activated carbon was ranged from 10 to $20\%$. Growth and enlargement of the root were improved by $10\%$ AC.

• 대한본초학회지
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• 제27권6호
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• pp.37-42
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• 2012

Objectives : Angelica Gigantis Radix is prescribed as the root of different Angelica species on the pharmacopoeia in Korea, Japan and China. Chemical components and their biological activities were also different according to their species. A study for the development of simple method to compare Angelica roots was needed. In order to classify them, the methods such as DNA sequencing analysis and taste sensor were applied to three Angelica species like Angelica gigas, Angelica acutiloba and Angelica sinensis. Methods : PCR amplification of intergenic transcribed spacer (ITS) region was performed using ITS1 and ITS4 primer from nine Angelica roots, and then nucleotide sequence was determined. Taste pattern of samples were measured using the taste-sensing system SA402B equipped with a sensing unit, which consists of artificial lipid membrane sensor probes of anionic bitterness, astringency, saltiness, umami, and cationic bitterness (C00, AE1, CT0, AAE, and AN0, respectively). Results : As a result of comparing the similarity of the ITS region sequences, A. sinensis was discriminated from the others (A. gigas and A. acutiloba). Equally this genetic result, A. gigas and A. acutiloba showed similar taste pattern as compared to A. sinensis. Sourness, bitterness, aftertaste of bitterness, astringency, and aftertaste of astringency of A. sinensis were significantly high as compared with A. gigas and A. acutiloba. In contrast, richness was significantly low. Conclusions : These taste pattern can be used as a way of comparison of Angelica species and this technic could be applied to establish a taste pattern marker for standardization of herbs in various purposes.