• Journal of Microbiology and Biotechnology
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• v.22 no.4
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• pp.555-562
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• 2012

PCR was performed to analyze the ${\beta}$-lactamase genes carried by ampicillin-resistant Vibrio spp. strains isolated from marine environments in Korea between 2006 and 2009. All 36 strains tested showed negative results in PCR with the primers designed from the nucleotide sequences of various known ${\beta}$-lactamase genes. This prompted us to screen new ${\beta}$-lactamase genes. A novel ${\beta}$-lactamase gene was cloned from Vibrio alginolyticus KV3 isolated from the aquaculture water of Geoje Island of Korea. The determined nucleotide sequence (VAK-3 ${\beta}$-lactamase) revealed an open reading frame (ORF) of 852 bp, encoding a protein of 283 amino acids (aa), which displayed low homology to any other ${\beta}$-lactamase genes reported in public databases. The deduced 283 aa sequence of VAK-3, consisting of a 19 aa signal peptide and a 264 aa mature protein, contained highly conserved peptide segments specific to class A ${\beta}$-lactamases including the specific amino acid residues STFK (62-65), SDN (122-124), E (158), and RTG (226-228). Results from PCR performed with primers specific to the VAK-3 ${\beta}$-lactamase gene identified 3 of the 36 isolated strains as V. alginolyticus, Vibrio cholerae, and Photobacterium damselae subsp. damselae, indicating the utilization of various ${\beta}$-lactamase genes including unidentified ones in ampicillin-resistant Vibrio spp. strains from the marine environment. In a mating experiment, none of the isolates transfered the VAK-3 ${\beta}$-lactamase gene to the Escherichia coli recipient. This lack of mobility, and the presence of a chromosomal acyl-CoA flanking sequence upstream of the VAK-3 ${\beta}$-lactamase gene, led to the assumption that the location of this new ${\beta}$-lactamase gene was in the chromosome, rather than the mobile plasmid. Antibiotic susceptibility of VAK-3 ${\beta}$-lactamase was indicated by elevated levels of resistance to penicillins, but not to cephalosporins in the wild type and E. coli harboring recombinant plasmid pKV-3, compared with those of the host strain alone. Phylogenetic analysis showed that VAK-3 ${\beta}$-lactamase is a new and separate member of class A ${\beta}$-lactamases.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.6C
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• pp.429-437
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• 2008

Multiple-input multiple-output (MIMO) techniques can be used for the spectral efficiency enhancement of the cellular systems, which can be categorized into spatial multiplexing (SM) and spatial diversity schemes. MIMO systems suffer a severe performance degradation due to the intercell interference from the adjacent cells as the mobile terminal moves toward the cell boundary. Therefore for the spectral efficiency enhancement, an appropriate transmission scheme for the given channel environment and reception scheme which can mitigate the intercell interference are required. In this paper, we propose an adaptive signal transmission/reception scheme for the spectral efficiency improvement of $M_R{\times}M_T$ MIMO systems, present the decision criteria for the adaptive operation of the proposed scheme, and demonstrate the performance gain. The proposed scheme performs adaptive transmission using spatial multiplexing and spatial diversity, and adaptive reception using maximal ratio combining (MRC) and intercell spatial demultiplexing (ISD) when the spatial diversity transmission is used at the transmitter. Spatial multiplexing/demultiplexing is performed at the high signal-to-interference ratio (SIR) range, and the transmit diversity in conjunction with the adaptive reception uses either conventional MRC or ISD which can mitigate the $M_R-1$ interference signals, based on the mobile location. For the performance evaluation of the proposed adaptive scheme, the probability density function (pdf) of the effective SIR for the transmission/reception methods in consideration are derived for $M_R{\times}M_T$ MIMO systems. Using the results, the average effective SIR and spectral efficiency are presented and compared with simulation results.

• Korean Journal of Ecology and Environment
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• v.52 no.3
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• pp.221-230
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• 2019

Barbel steed (Hemibarbus labeo) is a small freshwater fish species as semi-bottom dwellers distributed in eastern Asia. We carried out characterization of the cytochrome c oxidase subunit I (COI) gene from the mitochondrial DNA of H. labeo in the Sumjin River to identify the phylogenetic location of H. labeo in the genus Hemibarbus and Cyprinidae. Multiple alignment of the 577 bp COI sequence revealed high sequence homology (99~100%) between Seomjin River H. labeo. The nucleotide sequence similarity between H. labeo (HD1) and H. mylodon was 88.91% and that of H. longirostis was 88.81% among the three species found in Korea. In addition, the nucleotide sequence similarities of H. maculatus, H. meditus, H. umbrifer and H. barbus showed 98.97%, 97.20%, 96.87% and 98.85%, respectively. Phylogenetic analysis on seven species of the genus Hemibarbus showed that the H. labeo collected in this study formed two clades. One of which consisted of Hadong, Imsil, Kangjin. The other one formed a step with HD2, HD8 and HD9 of Hadong and the H. labeo reported in Busan, Asan and Seoul, Korea. Phylogenetic position of the H. labeo among Cyprinidae showed 0.143 for the evolutionary distance from Zacco platypus and 0.006 for the H. maculatus. In addition, the genetic position of the H. labeo among 28 species of Cyprinidae was found to be located in Group I, including Gobioninae fishes. The results of this study will provide key genetic information for the taxonomic comparison in Cyprinidae and study of model fish for pollution monitoring in freshwater environments.

• The Journal of the Korea institute of electronic communication sciences
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• v.17 no.1
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• pp.77-84
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• 2022

A submarine power cable is a transmission cable for carrying electric power below the surface of the water. Recently, submarine cables transfer power from offshore renewable energy schemes to shore, e.g. wind, wave and tidal systems, and these cables are either buried in the seabed or lie on the ocean floor, depending on their location. Since these power cables are used in the extreme environments, they are made to withstand in harsh conditions and temperatures, and strong currents. However, undersea conditions are severe enough to cause all sorts of damage to offshore cables, these conditions result in cable faults that disrupt power transmission. In this paper, we explore the design criteria for such cables and the procedures and challenges of installation, and cable transfer splicing system. The specification of submarine cable designed with 3 circuits of 154kV which is composed of the existing single circuit and new double circuits, and power capacity of 100MVA per cable line. The determination of new submarine cable burial depth and cable arrangement method with both existing and new cables are studied. We have calculated the permission values of cable power capacity for underground route, the values show the over 100MW per cable line.

• Land and Housing Review
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• v.14 no.4
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• pp.63-76
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• 2023

Investors should understand and actively consider factors like location, future value, policies, pricing, market trends, and their income, as these elements can shift with changing local, social, economic, and policy environments. This study seeks to clarify the impact of investment factors on the performance and reinvestment intentions of Sejong City investors by surveying those who have invested in real estate. This study employs a structural equation model with confirmatory factor analysis, focusing on four aspects: value, economic and policy, psychological, and financial. We find that the investment value factor has the largest impact on investment performance, indicating that investors prioritize the investment value of real estate in Sejong City. In addition, factors increasing asset value and expected satisfaction were significant, indicating that real estate investment in Sejong City yields high returns and investor satisfaction. with a positive outlook for future reinvestment.

• Journal of KIISE:Computing Practices and Letters
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• v.14 no.2
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• pp.143-157
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• 2008

Ubiquitous Web Services is able to be specified future Web Services technology for connecting with various application services in any device and network environments. The devices, in ubiquitous environment, have dynamic characteristic such as location and statuse. So, we must support methods of dynamic service discovery in ad-hoc network. There are many related works at transaction, security, QoS, semantic and Web Services composition with various fields. Recently, the studies are interested in the Ubiquitous by development of computing and network technology. However, they are an early stage. For this reason, in this paper, we propose a WSUN(Web Services on Universal Networks) for Ubiquitous Web Services. It is a SOA based framework. And this paper extracts necessity of WSUN environment from scenario. The framework is composed of US Broker(Universal Service Broker). It is designed for satisfying the conditions and supports dynamic service discovery using a US Registry (Universal Service Registry). Consequently. clients are able to discover and use Universal Service by protocol stack of the US Broker for Web Services. And it is a strong point which supports interoperability between heterogeneous networks.

• Journal of Cadastre & Land InformatiX
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• v.51 no.1
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• pp.53-65
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• 2021

C-ITS(Cooperative Intelligent Transport System) that pursues traffic safety and convenience uses various sensors to generate traffic information. Therefore, it is necessary to improve the sensor-related technology to increase the efficiency and reliability of the traffic information. Recently, the role of CCTV in collecting video information has become more important due to advances in AI(Artificial Intelligence) technology. In this study, we propose to identify and track dynamic objects(vehicles, people, etc.) in CCTV images, and to analyze and provide information about them in various environments. To this end, we conducted identification and tracking of dynamic objects using the Yolov4 and Deepsort algorithms, establishment of real-time multi-user support servers based on Kafka, defining transformation matrices between images and spatial coordinate systems, and map-based dynamic object visualization. In addition, a positional consistency evaluation was performed to confirm its usefulness. Through the proposed scheme, we confirmed that CCTVs can serve as important sensors to provide relevant information by analyzing road conditions in real time in terms of road infrastructure beyond a simple monitoring role.

• Journal of Applied Biological Chemistry
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• v.65 no.4
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• pp.429-438
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• 2022

The composition of crops reveal natural variation according to genetic characteristics and environmental factors such as the cultivated regions. For comparative investigation of the impact of genetic difference and environmental influence on the levels of bioactive components in rice seeds, 23 cultivars including indica, japonica, and tongil rice were grown in two location in Korea (Jeonju and Cheonan) for two years (2015 and 2016). Sixteen compounds consisting of tocopherols, tocotrienols, phytosterols, and policosanols were identified from 368 rice samples and the compositional data were subjected to data mining processes including principal component analysis and Pearson's correlation analysis. Under 4 different environmental conditions (Jeonju in 2015, Cheonan in 2015, Jeonju in 2016, Cheonan in 2016), the natural variability of rice seeds showed that the genetic background (indica vs japonica vs tongil) had more impact on the compositional changes of bioactive components compared to the environments. Especially, the results of correlation analysis revealed negative correlation between α-, β-tocopherols and γ-, δ-tocopherols as a representative genetic effect that did not changed by the environmental influence.

• Journal of Internet of Things and Convergence
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• v.9 no.1
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• pp.71-76
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• 2023

Recently, in order to store and manage big data, research and development of a high-performance storage system capable of stably accessing large data have been actively conducted. In particular, storage systems in data centers and enterprise environments use large amounts of SSD (solid state disk) to manage large amounts of data. In general, SSD uses FTL(flash transfer layer) to hide the characteristics of NAND flash memory, which is a medium, and to efficiently manage data. However, FTL's algorithm has a limitation in using DRAM more to manage the location information of NAND where data is stored as the capacity of SSD increases. Therefore, this paper introduces FTL policies that apply virtual memory to reduce DRAM resources used in FTL. The virtual memory-based FTL policy proposed in this paper manages the map data by using LRU (least recently used) policy to load the mapping information of the recently used data into the DRAM space and store the previously used information in NAND. Finally, through experiments, performance and resource usage consumed during data write processing of virtual memory-based FTL and general FTL are measured and analyzed.

• Journal of the Institute of Electronics and Information Engineers
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• v.54 no.1
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• pp.55-69
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• 2017

Recently, the IoT (Internet of Things) environment is being developed rapidly through network which is linked to intellectual objects. Through the IoT, it is possible for human to intercommunicate with objects and objects to objects. Also, the IoT provides artificial intelligent service mixed with knowledge of situational awareness. One of the industries based on the IoT is a car industry. Nowadays, a self-driving vehicle which is not only fuel-efficient, smooth for traffic, but also puts top priority on eventual safety for humans became the most important conversation topic. Since several years ago, a research on the recognition of the surrounding environment for self-driving vehicles using sensors, lidar, camera, and radar techniques has been progressed actively. Currently, based on the WAVE (Wireless Access in Vehicular Environment), the research is being boosted by forming networking between vehicles, vehicle and infrastructures. In this paper, a research on the recognition of a traffic signs on highway was processed as a part of the awareness of the surrounding environment for self-driving vehicles. Through the traffic signs which have features of fixed standard and installation location, we provided a learning theory and a corresponding results of experiment about the way that a vehicle is aware of traffic signs and additional informations on it.