• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.523-526
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• 2000

A novel cell surface display system was developed by employing the Escherichia coli outer membrane protein C (OmpC) as an anchoring motif. Poly-histidine (poly-His) peptides of 19, 32, 45, 84, and 162 amino acids (aa) could be successfully displayed by inserting them into the seventh exposed loop(L7) of OmpC. Recombinant cells displaying poly-His of 19, 32, 45, and 84 aa could absorb 18.9, 23.9, 26.1, and 32.0 ${\mu}mol$ of $Cd^{2+}$ per gram cell dry weight, respectively and therefore, would be useful as the biosorbents of heavy metals.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2012년도 추계 학술논문 발표대회
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• pp.169-170
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• 2012

It is analyzed that constructability of post-tensioning system applying HDPE greased strand that is greased and coated by high density polyethylene on a bare strand in reactor containment building. The improvement of corrosion resistance by greasing and HDPE coating on a strand makes transportation, handling and installation of tendon to be easier. Therefore, serial and repetitive process of post-tensioning composed of construction preparation, tendon installation, stressing and anchoring, grease injection could be improved parallel and lumping process of installation and grouting, stressing and anchoring.

• 대한치과교정학회지
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• 제26권4호
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• pp.341-348
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• 1996

저자는 마찰견인법으로 상악견치 후방견인시 저항원상실을 최소화하기 위해 근심 경사된 구치를 직립시키는 Molar Uprighting Spring의 작용기전을 응용하고 마찰견인법의 장점을 이용하여 임상적으로 사용이 용이하며 저항원상실이나 견치의 조절되지 못한 경사이동(uncontrolled tipping)등의 부작용을 최소화 할 수 있는 장치인 Molar Anchoring Spring(MAS)을 고안하였다. MAS를 임상에 적용한 결과 비교적 만족스러운 결과를 얻어 이에 장치를 소개하며 MAS의 저항원 조절능력과 견치의 이동양상을 평가하기 위하여 광탄성 모형을 제작한 후 주호선에 diameter 0.009" lumen size 0.030" NiTi closed coil spring만을 장착하여 250gm의 견인력이 발생하도록 한 경우와, 같은 NiTi closed coil spring으로 동일한 견인력 이 발생하도록 하고 .017" X .025" TMA wire로 $60^{\circ}$의 tip-back bend를 부여하여 수직교정력이 60-70gm이 되도록 제작한 MAS를 제1대구치 auxiliary tube에 삽입하고 견치 전방에 걸어준 경우를 비교실험 하여 견치견인시 초기응력분포를 관찰한 결과 다음과 같은 결과를 얻었다. 1. 주호선(main arch wire)에 저항원 조절을 위한 조치없이 견치에 수평견인력만을 가했을 때 구치부에 심한 저항원상실은 물론 견치의 원심이동양상도 조절되지 못한 경사이동(uncontrolled tipping)으로 나타났다. 2. 구치부의 저항원 조절을 위해 사용한 MAS는 견치의 원심이동 초기에 구치부 저항원 조절효과와 견치의 정출없는 조절된 경사이동(controlled tipping)을 유도할 수 있는 장치로 나타났다.

• KSBB Journal
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• 제26권3호
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• pp.199-205
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• 2011

Using tetrameric ${\beta}$-galactosidase as a model protein, anchoring motives were screened in Bacillus subtilis spore display system. Eleven spore coat proteins were selected considering their expression levels and the location in the spore coat layer. After chromosomal single-copy homologous integration in the amyE site of Bacillus subtilis chromosome, cotE and cotG were chosen as possible spore surface anchoring motives with their higher whole cell ${\beta}$-galactosidase activity. PAGE and Wester blot of extracted fraction of outer layer of purified spore, which express CotE-LacZ or CotG-LacZ fusion verified the existence of exact size of fusion protein and its location in outer coat layer of purified spore. ${\beta}$-galactosidase activity of spore with CotE-LacZ or CotG-LacZ fusion reached its highest value around 16~20 h of culture time in terms of whole cell and purified spore. After intensive spore purification with lysozyme treatment and renografin treatment, spore of BJH135, which expresses CotE-LacZ, retained only 1~2% of its whole cell ${\beta}$-galactosidase activity. Whereas spore of BJH136, which has cotG-lacZ cassette in the chromosome, retained 10~15% of its whole cell ${\beta}$-galactosidase activity, proving minor perturbation of CotG-LacZ, when incorporated in the spore coat layer of Bacillus subtilis compared to CotE-LacZ. Usage of Bacillus subtilis WB700, of which 7 proteases are knocked-out and thereby resulting in 99.7% decrease in protease activity of the host, did not prevent the proteolytic degradation of spore surface expressed CotG-LacZ fusion protein.

• 한국해양공학회지
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• 제11권4호
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• pp.205-212
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• 1997

The major subject of this paper is the conceptual design of a cable-buoy system based on mechanics of floating bodies and mooring lines. The goal is to present a systematic relevant analysis procedure for the conceptual design of oceanographic buoy systems. The dynamics of a free floating body with an anchoring line is studied and a model is proposed through the primary engineering.

• 해양환경안전학회지
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• 제24권6호
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• pp.662-669
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• 2018

최근 제주도를 비롯한 도서지역의 전력 사용량 증가 및 해상풍력 발전단지 개발 등으로 인해 해저 동력케이블의 신규 설치가 검토되고 있다. 해저에 설치되는 동력케이블의 보호를 위해서는 선박의 투묘, 주묘, 어로 작업 등에 대한 특성을 기반으로 매설 깊이를 산출해야 한다. 그러나 우리나라는 아직까지 해저 동력케이블 보호를 위한 대상 선박의 규모와 관련된 설계기준이 전무한 실정이다. 본 연구에서는 해저 동력케이블과 유사한 국내 해저배관의 보호를 위한 설계기준을 분석하고, 동력케이블의 설치 환경을 고려한 긴급 투묘의 형태별 분류를 토대로 위험도 매트릭스 모델을 개발하여 보호 대상 선박의 규모를 해당 해역을 통항하는 선박의 크기별 누적함수 규모에 따라 설계하였다. 해저 동력케이블 보호 기준에는 설치 해역의 수심과 조류 등의 환경 조건, 투묘와 주묘 등과 같은 해양사고 조건 등을 연계하였고, 선박의 운항 환경을 항계, 연안해역과 근해해역으로 구분하여 동력케이블의 구체적인 보호 대상 선박의 규모를 검토하였다. 대상 선박의 규모 결정에 대한 적정성 및 유용성 차원에서 완도에서 제주구간에 신설 예정인 제3호 해저 동력케이블에 적용하여 검증하였다. 이러한 해저 동력케이블과 해저배관 등의 보호를 위한 대상 선박의 선정 기준은 향후 매설깊이 설정에 따른 앵커 중량 선정은 물론 해저케이블 물리적 보호시스템 개발에도 활용될 것으로 기대된다.

• Steel and Composite Structures
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• 제10권1호
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• pp.87-108
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• 2010

A Steel Plate Shear Wall (SPSW) is a lateral load resisting system consisting of an infill plate located within a frame. When buckling occurs in the infill plate of a SPSW, a diagonal tension field is formed through the plate. The study of the tension field behavior regarding the distribution and orientation patterns of principal stresses can be useful, for instance to modify the basic strip model to predict the behavior of SPSW more accurately. This paper investigates the influence of torsional and out-of-plane flexural rigidities of boundary members (i.e. beams and columns) on the buckling coefficient as well as on the distribution and orientation patterns of principal stresses associated with the buckling modes. The linear buckling equations in the sense of von-Karman have been solved in conjunction with various boundary conditions, by using the Ritz method. Also, in this research the effects of symmetric and anti-symmetric buckling modes and complete anchoring of the tension field due to lacking of in-plane bending of the beams as well as the aspect ratio of plate on the behavior of tension field and buckling coefficient have been studied.

• Geomechanics and Engineering
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• 제16권3호
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• pp.321-329
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• 2018

Classic braced walls use struts and wales to minimize ground movements induced by deep excavation. However, the installation of struts and wales is a time-consuming process and confines the work space. To secure a work space around the retaining structure, an anchoring system works in conjunction with a braced wall. However, anchoring cannot perform well when the shear strength of soil is low. In such a case, innovative retaining systems are required in excavation. This study proposes an innovative earth-retaining wall that uses in situ soil confined in dual sheet piles as a structural component. A numerical study was conducted to evaluate the stability of the proposed structure in cohesionless dry soil and establish a design chart. The displacement and factor of safety of the structural member were monitored and evaluated. According to the results, an increase in the clearance distance increases the depth of safe excavation. For a conservative design to secure the stability of the earth-retaining structure in cohesionless dry soil, the clearance distance should exceed 2 m, and the embedded depth should exceed 40% of the wall height. The results suggest that the proposed method can be used for 14 m of excavation without any internal support structure. The design chart can be used for the preliminary design of an earth-retaining structure using in situ soil with dual steel sheet piles in cohesionless dry soil.

• Journal of Microbiology and Biotechnology
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• 제17권4호
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• pp.677-680
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• 2007

To analyze a cotG-based Bacillus subtilis spore display system directly, $GFP_{UV}$ was expressed on the surface of Bacillus subtilis spores. When $GFP_{UV}$ was fused to the C-terminal of the cotG structural gene and expressed, the existence of a $CotG-GFP_{UV}$ fusion protein on the B. subtilis spore was confirmed by flow cytometry confocal microscopic analysis. When the cotG anchoring motif was deleted, no fluorescence emission was observed under flow cytometry and confocal microscopic analysis from the purified spore, confirming the essential role of CotG as an anchoring motif. This $GFP_{UV}$ displaying spore might be used for another signaling application triggered by intracellular or extracellular stimuli.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 1998년도 봄 학술발표회논문집(II)
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• pp.559-565
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• 1998

Flexural tests on 2.4m long reinforced concrete beams with epoxy-bonded GFRP plates are reported in these tests. The selected experimental variables are strengthening plate length, plate thickness, plate width and the method of anchoring the plate ends. The effects of these variables in overall behavior are discussed. The results generally indicate that the flexural strength of strengthened beams is increased. The ductile behavior of tested beams in inversely proportional to the plate thickness, plate width. The use of an U-jacket plate provided a proper anchorage system and improved the ductility of beams.