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Bacterial Surface Display of $GFP_{UV}$ on Bacillus subtilis Spores  

Kim, Jung-Hyung (Department of Chemical Engineering, Dong-A University)
Roh, Chang-Hyun (Institute for Molecular Biology and Genetics, and School of Chemical Engineering, Seoul National University)
Lee, Chang-Won (Institute for Molecular Biology and Genetics, and School of Chemical Engineering, Seoul National University)
Kyung, Do-Hyun (Institute for Molecular Biology and Genetics, and School of Chemical Engineering, Seoul National University)
Choi, Soo-Keun (Genofocus Inc.)
Jung, Heung-Chae (Genofocus Inc.)
Pan, Jae-Gu (Genofocus Inc.)
Kim, Byung-Gee (Institute for Molecular Biology and Genetics, and School of Chemical Engineering, Seoul National University)
Publication Information
Journal of Microbiology and Biotechnology / v.17, no.4, 2007 , pp. 677-680 More about this Journal
Abstract
To analyze a cotG-based Bacillus subtilis spore display system directly, $GFP_{UV}$ was expressed on the surface of Bacillus subtilis spores. When $GFP_{UV}$ was fused to the C-terminal of the cotG structural gene and expressed, the existence of a $CotG-GFP_{UV}$ fusion protein on the B. subtilis spore was confirmed by flow cytometry confocal microscopic analysis. When the cotG anchoring motif was deleted, no fluorescence emission was observed under flow cytometry and confocal microscopic analysis from the purified spore, confirming the essential role of CotG as an anchoring motif. This $GFP_{UV}$ displaying spore might be used for another signaling application triggered by intracellular or extracellular stimuli.
Keywords
Bacillus subtilis; cotG; $GFP_{UV}$; spore; surface display;
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Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 7  (Related Records In Web of Science)
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