Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Bacterial Surface Display of $GFP_{UV}$ on Bacillus subtilis Spores

  • Kim, Jung-Hyung (Department of Chemical Engineering, Dong-A University) ;
  • Roh, Chang-Hyun (Institute for Molecular Biology and Genetics, and School of Chemical Engineering, Seoul National University) ;
  • Lee, Chang-Won (Institute for Molecular Biology and Genetics, and School of Chemical Engineering, Seoul National University) ;
  • Kyung, Do-Hyun (Institute for Molecular Biology and Genetics, and School of Chemical Engineering, Seoul National University) ;
  • Choi, Soo-Keun (Genofocus Inc.) ;
  • Jung, Heung-Chae (Genofocus Inc.) ;
  • Pan, Jae-Gu (Genofocus Inc.) ;
  • Kim, Byung-Gee (Institute for Molecular Biology and Genetics, and School of Chemical Engineering, Seoul National University)
  • Published : 2007.04.30
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Abstract

To analyze a cotG-based Bacillus subtilis spore display system directly, $GFP_{UV}$ was expressed on the surface of Bacillus subtilis spores. When $GFP_{UV}$ was fused to the C-terminal of the cotG structural gene and expressed, the existence of a $CotG-GFP_{UV}$ fusion protein on the B. subtilis spore was confirmed by flow cytometry confocal microscopic analysis. When the cotG anchoring motif was deleted, no fluorescence emission was observed under flow cytometry and confocal microscopic analysis from the purified spore, confirming the essential role of CotG as an anchoring motif. This $GFP_{UV}$ displaying spore might be used for another signaling application triggered by intracellular or extracellular stimuli.

Keywords

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