• 제목/요약/키워드: Anaplasma

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대전 주택가 산책로 진드기의 인수공통전염병 병원체 감염실태 조사 (Isolation and identification of tick-borne pathogens in hard ticks collected in Daejeon)

  • 한소영;성선혜;서진우;김종호;이석주;유상식
    • 한국동물위생학회지
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    • 제44권2호
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    • pp.93-102
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    • 2021
  • In this study, a total of 9,449 hard ticks were collected once a month from April to October 2020 from a neighborhood park in Daejeon by flagging & dragging method and CO2 manned trap method. The collected ticks were classified according to the Yamagutsi search table using a stereoscopic microscope and molecular biological analysis of four pathogens (SFTSV, Anaplasma spp., Ehrlichia spp., Borrellia spp.). As a result of the study, Haemaphysalis longicornis were collected the most in all areas of the five boroughs at a rate of 82 to 96 percent, while adults were collected the most in May to July, nymphs were collected the most in April to June, and larvae from August to October at a rate of 78 percent to 98 percent. In pathogens, three cases of SFTSV were detected, showing a minimum infection rate (MIR) of 0.46%, while Anaplasma spp. and Ehrlichia spp. were detected one each, with 0.15% and Borrelia spp. with a minimum infection rate of 0.46%. The detected SFTSV showed 99.9% homogeneity with the KF781490 detected in Cheongwon-gun, Chungbuk Province, Anaplasma spp. showed 99.0% homogeneity with JN990105 detected in China, and Erhlichia spp. showed 98.9% genetic similarity with U96436 separated from the U.S. In this study, the distribution status and pathogen infection rate of the hard ticks in the Daejeon area are analyzed and provided as basic data for the prevention of the hard tick-borne infectious disease.

First Evaluation of an Outbreak of Bovine Babesiosis and Anaplasmosis in Southern Brazil Using Multiplex PCR

  • Canever, Mariana Feltrin;Vieira, Luisa Lemos;Reck, Carolina;Richter, Luisa;Miletti, Luiz Claudio
    • Parasites, Hosts and Diseases
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    • 제52권5호
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    • pp.507-511
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    • 2014
  • Outbreaks of tick-borne disease cases in Santa Catarina, Brazil are known, but the presence of the pathogen DNA has never been determined. In this study, the first survey of Anaplasma marginale, Babesia bigemina, and Babesia bovis DNA on blood samples of 33 cattle from an outbreak in Ponte Alta Municipality, Santa Catarina, Brazil, has been carried out. A multiplex PCR detected 54.5% of animals were co-infected with 2 or 3 parasites, while 24.2% were infected with only 1 species. The most prevalent agent was B. bigemina (63.6%) followed by A. marginale (60.6%). This is the first report of tick-borne disease pathogens obtained by DNA analysis in Southern Brazil.

전라북도 동부지역 사슴에서 진드기매개성 병원체에 대한 감염 실태 조사 (A survey for tick-borne disease agents from farm deer in the eastern area of Jeonbuk)

  • 엄성심;고원석;허철호;배정준
    • 한국동물위생학회지
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    • 제29권2호
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    • pp.103-110
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    • 2006
  • Ticks cause economic losses to the deer industry by decreasing the growth and production of the farmed animals. The mediation of ticks affects humans and animals by causing contagious disease both directly and indirectly. Blood from farmed deer from the areas near Jangsu branch was collected for screening of infectious protozoa and rickettsial disease. Seventy deer blood samples were collected from 30 different deer farms located in Jinan, Jangsu and Muju. This blood samples were used for blood slide smear examination and hematological analysis. DNA from these samples was extracted and was used for PCR analysis for detection of gene fragments of Theileria spp, Babesia spp, Anaplasma spp and Ehrlichia spp. In the blood slide smear examination and PCR analysis all samples did not show presence of protozoal and rickettsial diseases. Eight blood samples showed anemia, 1 sample showed iron deficiency and 7 samples showed regenerative anemia. Results for PCR analysis showed 2 samples were positive for T orientalis. All DNA samples were negative for Babesia spp, Anaplasma spp, and Ehrlichia spp.

재래산양에서 주혈미생물의 감염실태 및 실험적 치료시험 (Prevalence of blood parasites infection and experimental treatment in Korean native goats)

  • 허부홍;전창권;이희문;김용수;김윤태;이정원;최승옥;안병목;송희종
    • 한국동물위생학회지
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    • 제18권3호
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    • pp.42-52
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    • 1995
  • Anaplasmosis is most important tick-borne rickettsial diseases of domestic ruminant. with Anaptasma spp. as their respective causal agents. In order to the survey prevalence of anaplasmosis in Korean native goats, we examined the Giemsa's blood filrms from 552 grazing and 188 nongrazing-goats in Chonbuk area Namwon, Imsil and Sunchang. The conclusive diagnosis was made by observing the characteristic marginal or central bodies in the red blood cells. The infection rate of grazine and nongrazing-goats with Anaplasma spp. was 71.7% and 8.5%, respectively. In order to study about the therapeutic effects of drug for 40 grazing-oats infected with Anaptasma spp. in mountain-area, Berenil(diminazene acetate) was jntramuscularly injected (0.5㎖ /10kg B.W.). After 1 month, we don't detected the parasite in the blood films from 33 goats(82.5%), but no change of significant hematological values(PCV, ESR, WBC, RBC, MCH, MCHC, PLT, MPV, PDW and HB) was observed. These results indicated that the infection rate of Anaplasma spp. was higher on glazing than nongrazing-goats and Berenil as an antianaplasmal drug is recommended.

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경북지역의 흑염소 내부기생충 감염률 조사 (A survey on the prevalence of internal parasites in the Korean indigenous goats of Kyungbuk area)

  • 박노찬;도재철;김수웅;송해범
    • 한국동물위생학회지
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    • 제20권4호
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    • pp.349-358
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    • 1997
  • From December 1995 to July 1997, a total of 546 Korean indigenous goats were examined for the presence of internal parasites by parenchymal organs and fecal examinations. Four hundreds seventy eight(87.5%) of the goats were infected with 4 classes and 12 parasitic species. The nematodes were Haemonchus contortus(57.5%), Oesophagostomun venulosum (52.6%), Strongyloides papillosus(48.4%), Ostertagia sp(28.4%), Trichostrongylus colubriformis(17.0%), and Trichuris ovis(13.2%). The trematodes were Paramphistomum sp(17.6%), Fasciola hepatica(15.4%), and Eurytrema Pancreaticum (8.8%). The cestode was Moniezia expansa(16.7%), and the protozoa were Eimeria sp(74.9%) and Toxoplasma gondii(8.6%). The incidence of single or mixed infections among 12 parasitic species were one species infection (6.2%), 2 species(11.5%), 3 species(19.6%), 4 species(24.9%), 5 species(17.8%), 6 species(5.9 %), and 7 species(1.6), respectively. The level of infection according to the age was considerably higher under one year(97.2%) than between one and two years(89.7%), and over two years(69.3%) Among 12 parasites, seasonal pattern was remarkably observed. Most paracites were detected at the highest level in the Winter, whereas Strongyloides papillosus and Paramphistomum sp were in the Spring, and Ostertagia sp and Fasciola hepatica were in the Autumn. Infection rates of Anaplasma sp were 8.6% of 210 goats by blood smear method, and 51.0% by complement fixation test.

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대전시와 강원도에서 심장사상충증, 아나플라즈마증, 보렐리아증 및 얼리키아증의 혈청학적 발생율 조사 (Seroprevalence of Dogs with Dirofilaria immitis, Anaplasma phagocytophilum, Borrelia burgdorferi and Ehrlichia canis Infection in the Daejeon City and Kangwon Province)

  • 김태훈;김연희;최준혁;박형진;정대욱;김덕환;송근호
    • 한국임상수의학회지
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    • 제27권6호
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    • pp.631-634
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    • 2010
  • 본 연구는 대전시와 강원도에서 사육중인 개 252두 (암컷: 127두, 수컷: 125두, 실외견: 147두, 실내견: 105두)를 대상으로 ELISA kit (SNAP 4Dx, IDEXX Lab. USA)를 이용하여 심장사상충증, 아나플라즈마증, 보렐리아증 및 얼리키아증에 대한 혈청학적 방법으로 발생율을 조사하였다. 아나플라즈마증은 암컷에서 실내견보다 실외견에서 유의성 있는 높은 감염율을 나타내었다. 또한 얼리키아증에서는 연령별로 분석한 결과, 실외견에서 실내견보다 4세미만, 4-7세사이, 7세 이상에서 각각 유의성 있는 높은 감염율을 나타내었다. 본 연구는 임상가들에게 유용한 임상자료로 활용될 것으로 판단된다.

도입우에 대한 진드기매개질병의 감염실태에 관한 조사연구 (Studies on Incidence of Tick - borne Diseases in Imported Cattle in Korea)

  • 서명득;김용희;강영배;강승원
    • 대한수의사회지
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    • 제18권4호
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    • pp.19-32
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    • 1982
  • 진드기매개질병에 대한 예방대책을 수립하고 저 국내에 기도입된 소에서 출산하여 처음방목 되는 소와 신규로 도입되어 처음 방목되는 유우 및 육우를 대상으로 하여 각 지역별로 주혈원충의 감염상과 이 원충에 의한 질병의 발생실태를 조사하여 다음과 같은 결론을 얻었다. 1. 국내 도입우의 진드기매개질병에 관여하는 병원체는 Theileria sergenti, Babesia ovata Anaplasma marginate 및 Anaplasma centrale로 확인되었다. 2. 내륙의 평야지대인 경기, 충남 및 경남지역에서는 T. sergenti, 충북과 전남지역에서는 T. sergenti와 B.ovata 그리고 전북지역 (고냉지대)에서는 T. sergenti, B.ovata및 A.centrale가 각각 관여하고 있었다. 3. 대관령지역에서는 T. sergenti만이 관여하고 있었다. 4. 제주지역에서는 T. sergenti, B.ovata 및 A. .marginate가 관여하고 있었다. 5. 내륙의 진드기 서식이 높은 지역에서는 방목후 4 - 5주째에 감염강도 $109.9\~174.7$을 나타내었고 이 시기에 발병증상을 보였다. 그리고 진드기 서식이 낮은 지역에서는 방목후 $5\~6$주째에 감염강도 $31.3\~40$을 나타내었고 발병증상 없이 내과하였다. 6. 대관령 지역에서는 방목후 8주째에 감염강도 54.4-69.2를 나타내었고 이 시기에 발병 증상을 보였다. 7. 제주지역에서는 방목후 $3\~4$주째에 감염강도 33.9-42.7을 나타내었고 이 시기를 전후하여 발병증상을 보였다 . 8. 국내에 있어서 진드기 섭식이 낮은 지역과 높은 지역을 설정하였다.

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