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http://dx.doi.org/10.3347/kjp.2014.52.5.507

First Evaluation of an Outbreak of Bovine Babesiosis and Anaplasmosis in Southern Brazil Using Multiplex PCR  

Canever, Mariana Feltrin (Laboratorio de Bioquimica de Hemoparasitas e Vetores, Centro de Ciencias Agroveterinarias, Universidade do Estado de Santa Catarina)
Vieira, Luisa Lemos (Laboratorio de Bioquimica de Hemoparasitas e Vetores, Centro de Ciencias Agroveterinarias, Universidade do Estado de Santa Catarina)
Reck, Carolina (Laboratorio de Bioquimica de Hemoparasitas e Vetores, Centro de Ciencias Agroveterinarias, Universidade do Estado de Santa Catarina)
Richter, Luisa (Companhia Integrada de Desenvolvimento Agricola de Santa Catarina)
Miletti, Luiz Claudio (Laboratorio de Bioquimica de Hemoparasitas e Vetores, Centro de Ciencias Agroveterinarias, Universidade do Estado de Santa Catarina)
Publication Information
Parasites, Hosts and Diseases / v.52, no.5, 2014 , pp. 507-511 More about this Journal
Abstract
Outbreaks of tick-borne disease cases in Santa Catarina, Brazil are known, but the presence of the pathogen DNA has never been determined. In this study, the first survey of Anaplasma marginale, Babesia bigemina, and Babesia bovis DNA on blood samples of 33 cattle from an outbreak in Ponte Alta Municipality, Santa Catarina, Brazil, has been carried out. A multiplex PCR detected 54.5% of animals were co-infected with 2 or 3 parasites, while 24.2% were infected with only 1 species. The most prevalent agent was B. bigemina (63.6%) followed by A. marginale (60.6%). This is the first report of tick-borne disease pathogens obtained by DNA analysis in Southern Brazil.
Keywords
Babesia bovis; Babesia bigemina; Anaplasma marginale; multiplex-PCR; Brazil;
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