• 제목/요약/키워드: Analytical Methodology

검색결과 564건 처리시간 0.026초

A demonstration of the H3 trimethylation ChIP-seq analysis of galline follicular mesenchymal cells and male germ cells

  • Chokeshaiusaha, Kaj;Puthier, Denis;Nguyen, Catherine;Sananmuang, Thanida
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제31권6호
    • /
    • pp.791-797
    • /
    • 2018
  • Objective: Trimethylation of histone 3 (H3) at 4th lysine N-termini (H3K4me3) in gene promoter region was the universal marker of active genes specific to cell lineage. On the contrary, coexistence of trimethylation at 27th lysine (H3K27me3) in the same loci-the bivalent H3K4m3/H3K27me3 was known to suspend the gene transcription in germ cells, and could also be inherited to the developed stem cell. In galline species, throughout example of H3K4m3 and H3K27me3 ChIP-seq analysis was still not provided. We therefore designed and demonstrated such procedures using ChIP-seq and mRNA-seq data of chicken follicular mesenchymal cells and male germ cells. Methods: Analytical workflow was designed and provided in this study. ChIP-seq and RNA-seq datasets of follicular mesenchymal cells and male germ cells were acquired and properly preprocessed. Peak calling by Model-based analysis of ChIP-seq 2 was performed to identify H3K4m3 or H3K27me3 enriched regions ($Fold-change{\geq}2$, $FDR{\leq}0.01$) in gene promoter regions. Integrative genomics viewer was utilized for cellular retinoic acid binding protein 1 (CRABP1), growth differentiation factor 10 (GDF10), and gremlin 1 (GREM1) gene explorations. Results: The acquired results indicated that follicular mesenchymal cells and germ cells shared several unique gene promoter regions enriched with H3K4me3 (5,704 peaks) and also unique regions of bivalent H3K4m3/H3K27me3 shared between all cell types and germ cells (1,909 peaks). Subsequent observation of follicular mesenchyme-specific genes-CRABP1, GDF10, and GREM1 correctly revealed vigorous transcriptions of these genes in follicular mesenchymal cells. As expected, bivalent H3K4m3/H3K27me3 pattern was manifested in gene promoter regions of germ cells, and thus suspended their transcriptions. Conclusion: According the results, an example of chicken H3K4m3/H3K27me3 ChIP-seq data analysis was successfully demonstrated in this study. Hopefully, the provided methodology should hereby be useful for galline ChIP-seq data analysis in the future.

회복력 (Resilience) 개념 개발 (Concept Development of Resilience)

  • 김혜성
    • 대한간호학회지
    • /
    • 제28권2호
    • /
    • pp.403-413
    • /
    • 1998
  • The Resilience is described as the personal capacity which brings psychosocial comeback. The role of nursing is to do its best to rehabilitate patients and to explore the individual in order to promote patients psychosocial change. However, as the current nursing is heavily physical nursing oriented, the identity of the nursing would be lost. Therefore this researcher reviewed if the concept of resilience can be applied to the nursing after examing the concept of resilience by Documents and Fieldwork. The methodology of this research is Hybrid Model developed by Schwartz-Barcott and Kim for the concept development and analysis. The process and procedure consist of The Theoretical Phase, The Fieldwork Phase and The Final Analytical Phase in accodance with the Hybrid Model. The followings the summary of the Research. 1. The Concept of Resilience Finally Analyzed by Documents and Fieldwork (1) The Redefinition of Resilience The resilience is the latent psychosocial capacity which minimize the negative emotion and promote the adaptation under adversity. Resilience appears as cognitive, emotional and behavioral response in the course of changing from negative response to positive response through the interaction of the individual and the enviroments in a given time. Resilience changes and decreases according to time and situation and it can be nurtured. Resilience is the higher concept including hardiness, sense of coherence and self-strength which maintain the health under stress. (2) The Attribute of Resilience The attribute of resilience was devided into psychological and social dimension. In psychological attributes, there are admittion of reality of situation, denial of negative emotion, desire to live, responsibility, confidence, courage, hope, pursuit of positive meaning, identification and pursuit of goal, self-esteem, reception, spontaneity, planning, positiveness, will power, flexibility and creativity. In social attributes, there are a sense of belonging, perception of social support and active social relations. (3) The Process of Resilience There are 4 resilience phases which were the process minimizing the possibility of the negative chain reactions under adversity, the process minimizing the negative emotion under adversity, the process gaining the desire to live and the process exposing the active social relations. 2. The Application Possibility of Resilience Concept to Nursing The resilience concept is the psychosocial capacity with which an individual manages adversity. As many nursing scientists have developed nursing theory based on this capacity and the identification of nursing has been established in this field, resilience is not the new conception in nursing. However, since resilience appears in the attributes related with the resilience process concretely, it would help a lot when nurses execute psychosocial nursing.

  • PDF

Rogers 이론에 근거한 가족양상 사정지침개발 (Development of a Family Pattern Appraisal to Guide a Rogerian Nursing Practice)

  • 이광옥;한영란;김희정
    • 대한간호학회지
    • /
    • 제25권4호
    • /
    • pp.751-773
    • /
    • 1995
  • We, clinical nurse specialists practising and guiding student practice in a Community health nursing clinic, wanted to develop a family pattern appraisal consistent with Rogers' conceptual system, the nursing model guiding our practice. We use Rogers' model because it is harmonious with the traditional Korean view of the one human, natural and cosmic world. The purpose of our research was to contribute to science - based nursing practice, not only, one helpful model, but also a model of how to use, in guiding practice, a conceptual system which reflects nurse practitioners' philosophy of nursing, is intellectually satisfying, and enriches meaning in daily nursing life. The research objectives were to review the literature on Rogers' model and analyse it according to Kim's five - level analytical framework, to explore Rogers' definition of family, to review appraisals based on Rogers' model, and to develop a family appraisal which is culturally appropriate for use in our community. This work including the use of the appraisal and its refinement with families in our practice which was done during 1994 and 1995, in Seoul, in the Capital of the Republic of Korea. At the highest level of analysis, Rogers conceptual system emphasizes acausality and multidimentional meaning ; the world view is characterized by process, movement and wholeness. The epistemology Is one of holism and the knowledge base includes all forms of experience, from sensory to mystical, objective, and subjective. At the metaparadigm level, nursing focuses on the unitary human being and the environment. At the level of nursing philosophy, the model identifies human being, nursing, nurse, and illness and health. At the paradigm level the model assumes the irriducibility of the human to parts, noncausality and continual change. Rogers' practice methodology consists of pattern manifestation appraisal and deliberative mutual patterning. Under-standing patterns and patterning of people is the key to helping them achieve their potential. At the theory level, the basic assumptions, key concepts, and homeodynamic principles were identified. Rogers states the family energy field is an undividable, four-dimensional negentropic energy field which is in a larger envircinmental field show-ing such characteristics as cannot be predicted by knowledge of individual family members. Based on the word of Rogers scholars, we chose Rogers' correlates of patterning to understand the family unit as a whole-frequency, rhythms, motion, time perception, sleeping-waking beyond waking, pragmatic -imaginative-visionary to develop the appraisal. We, also used some of Barrel's (1988) criteria including interpersonal network and professional health care access and use, and Cordon's (1982) criteria including self perception - self concept modified to fit the family. Our family Pattern appraisal included 1. Influencirg data, 2. Professional health care access and use, 3. Family self perception-self concept, 4. Family interpersonal network, 5. Sleep-wake-be-yond waking, 6. Pragmatic-imaginary-visionary, 7. Family frequency and rhythm, 8. Family motion, 9. Family time perception. The appraisal was used with four families and modified to eliminate overlap and to make it possible for the family member to express themselves more easily. We plan to gain more experience with the appraisal toward further development of the tool.

  • PDF

GCP로 보강된 연약지반의 해석적 거동분석 (Analysis on the Analytical Behavior of Soft Ground Reinforced with Granular Compaction Piles)

  • 김민석;나승주;양열호;김대현
    • 한국지반신소재학회논문집
    • /
    • 제15권3호
    • /
    • pp.27-37
    • /
    • 2016
  • 국내 GCP공법은 많은 선행연구가 진행되었으나, 설계자의 경험에 의해 의존하여 설계되고 있는 실정이므로 파괴사례가 종종 보고되고 있다. 이에 따라 명확한 파괴 원인규명 및 파괴예방대책 수립이 어려운 실정이다. 따라서 본 연구에서는 GCP공법의 합리적인 설계법의 제안을 위한 기초단계로써, GCP에 사용되는 최적배합비를 대형직접전단시험을 통해 결정하고, 내부마찰각의 변화에 따른 응력분담비의 변화 및 지반의 수직/수평 침하거동을 수치해석을 통하여 분석하였다. 직접전단 실험결과 쇄석과 모래의 최적배합비는 70:30으로 평가되었다. 수치해석결과 내부마찰각이 증가할수록 응력분담비가 증가하나 일정한 값으로 수렴하고 침하량이 감소하며, 최적배합비로 시공할 경우 측방유동 및 히빙현상의 감소를 유도할 것으로 판단된다.

설계시간계수 및 Simulation 기반 철도역사 이용시설 LOS 분석 (The LOS Analysis of Railway Station Facilities Based on Design Hourly Factor and Simulation)

  • 오태호;이선하;천춘근;김은지;유병영
    • 한국ITS학회 논문지
    • /
    • 제15권3호
    • /
    • pp.105-117
    • /
    • 2016
  • 최근 국내철도역사 인프라의 다변화(환승, 쇼핑 등)로 이용객이 증가하고 있는 반면, 이를 고려하지 못한 철도역사 설계로 이용객의 철도역사에 대한 만족도 수준은 낮아지고 있는 실정이다. 예를들어, KTX 광주송정역의 경우 2015년 개통 이후 이용객이 약 3배 이상 증가하여 역사 내 이용객 혼잡과 같은 불편함을 초래하고 있다. 본 연구에서는 철도역사 이용시설의 적정규모 산정을 위하여 도로공학의 설계시간계수 개념과 보행 시뮬레이션을 활용한 분석을 시행하였다. 이를 위해서는 분석과정을 크게 2단계로 구분하였다. 1단계는 교통학적 측면에서 도로설계 시 이용되는 설계시간계수의 방법론을 인용한 철도역사 이용객 산출, 2단계는 보행 시뮬레이션을 통하여 철도역사 시설물별 서비스수준 분석을 시행하였다. 분석결과 보행 시뮬레이션 활용으로 철도역사 시설물별 서비스수준 분석 가능여부가 검증되어 향후 역사 설계 시 시설물별 적정 서비스수준 따른 면적 제시가 가능할 것으로 판단된다.

도시계획과 연계한 공간구조의 변화 특성 분석 - 공간통계기법을 이용하여 (Analysis on Change Characteristics of Spatial Structure Related with Urban Planning : Using Spatial Statistical Method)

  • 서경민;김호용;이성호;권태호
    • 한국지리정보학회지
    • /
    • 제17권2호
    • /
    • pp.1-14
    • /
    • 2014
  • 본 연구에서는 효율적인 도시 관리를 위하여 대구시 도시공간구조의 시계열적 공간변화 특성을 도시계획과 연계하여 분석하였다. Getis-Ord $G_i^*$ 방법론을 활용하여 1970년부터 2010년까지 대구시의 도시성장과정을 분석한 결과, 대구시는 도시성장단계 중에서 역도시화 단계에 해당하는 것을 확인하였다. 하지만 대구시는 도시계획적 차원에서 다핵도시를 지향함으로써 역도시화가 나타난 2000 이후의 도시성장단계는 더 이상 전통적인 이론으로 설명하기 어려웠다. 이에 2000년 이후 다핵권역의 세부적인 공간구조변화를 분석하기 위하여 인구, 지가, 고용 요인의 변화를 바키측 정치를 이용하여 분석하였다. 분석결과 대구시는 다핵화가 진행 중이었으며 도심권역과 칠곡권역은 분산형, 안심 달서권역은 집중형, 성서권역은 집중 분산형태로의 공간구조변화가 나타났다. 지역에 따라 다른 도시공간구조를 나타내는 도시의 효율적인 관리를 위해서는 권역별 특성을 고려한 도시계획과 개발계획이 필요한 것으로 판단된다.

Target Identification for Metabolic Engineering: Incorporation of Metabolome and Transcriptome Strategies to Better Understand Metabolic Fluxes

  • Lindley, Nic
    • 한국미생물생명공학회:학술대회논문집
    • /
    • 한국미생물생명공학회 2004년도 Annual Meeting BioExibition International Symposium
    • /
    • pp.60-61
    • /
    • 2004
  • Metabolic engineering is now a well established discipline, used extensively to determine and execute rational strategies of strain development to improve the performance of micro-organisms employed in industrial fermentations. The basic principle of this approach is that performance of the microbial catalyst should be adequately characterised metabolically so as to clearlyidentify the metabolic network constraints, thereby identifying the most probable targets for genetic engineering and the extent to which improvements can be realistically achieved. In order to harness correctly this potential, it is clear that the physiological analysis of each strain studied needs to be undertaken under conditions as close as possible to the physico-chemical environment in which the strain evolves within the full-scale process. Furthermore, this analysis needs to be undertaken throughoutthe entire fermentation so as to take into account the changing environment in an essentially dynamic situation in which metabolic stress is accentuated by the microbial activity itself, leading to increasingly important stress response at a metabolic level. All too often these industrial fermentation constraints are overlooked, leading to identification of targets whose validity within the industrial context is at best limited. Thus the conceptual error is linked to experimental design rather than inadequate methodology. New tools are becoming available which open up new possibilities in metabolic engineering and the characterisation of complex metabolic networks. Traditionally metabolic analysis was targeted towards pre-identified genes and their corresponding enzymatic activities within pre-selected metabolic pathways. Those pathways not included at the onset were intrinsically removed from the network giving a fundamentally localised vision of pathway functionality. New tools from genome research extend this reductive approach so as to include the global characteristics of a given biological model which can now be seen as an integrated functional unit rather than a specific sub-group of biochemical reactions, thereby facilitating the resolution of complexnetworks whose exact composition cannot be estimated at the onset. This global overview of whole cell physiology enables new targets to be identified which would classically not have been suspected previously. Of course, as with all powerful analytical tools, post-genomic technology must be used carefully so as to avoid expensive errors. This is not always the case and the data obtained need to be examined carefully to avoid embarking on the study of artefacts due to poor understanding of cell biology. These basic developments and the underlying concepts will be illustrated with examples from the author's laboratory concerning the industrial production of commodity chemicals using a number of industrially important bacteria. The different levels of possibleinvestigation and the extent to which the data can be extrapolated will be highlighted together with the extent to which realistic yield targets can be attained. Genetic engineering strategies and the performance of the resulting strains will be examined within the context of the prevailing experimental conditions encountered in the industrial fermentor. Examples used will include the production of amino acids, vitamins and polysaccharides. In each case metabolic constraints can be identified and the extent to which performance can be enhanced predicted

  • PDF

GMM(Gaussian Mixture Model)을 적용한 영상처리기법의 연속류도로 사고 자동검지 알고리즘 개발 (Development of the Algofithm for Gaussian Mixture Models based Traffic Accident Auto-Detection in Freeway)

  • 오주택;임재극;여태동
    • 대한교통학회지
    • /
    • 제28권3호
    • /
    • pp.169-183
    • /
    • 2010
  • 영상기반의 교통정보수집시스템은 관리 및 운영상의 한계를 보이고 있는 기존의 루프검지기의 역할을 대체하는 검지기로써의 역할 뿐만 아니라 다양한 교통류의 정보를 제공하고 관리할 수 있으며, 교통사고의 발생전과 후의 순차적인 상황을 정확히 기록하고, 이 자료를 통해 발생된 교통사고의 사고 매커니즘을 객관적이고 명확하게 조명하고 분석하는 것은 교통사고 처리에 있어서 중요한 부분을 차지함으로서, 여러 나라에서 보급 활용되고 있다. 본 논문에서는, 기존 기술들이 연속류 도로의 특성인 속도변화, 교통량 변화, 점유율 변화와 같은 교통류 흐름을 반영하여 1차 예비판단을 실시하였다. 또한, 1차 예비판단된 경우 영상추출 및 처리를 통해 최종 사고판단을 실시하게 된다. 이 때, 도로상의 다양한 환경적 변화로 인해 극복하기 어려운 차량의 객체추출, 객체분리, 추적 등의 정확성을 확보하기 위해서 계산속도와 정확도 측면에서 우수함을 보이고 있는 Adaptive GMM(Gaussian Mixture Model) 기반으로 실시하였으며, 환경적인 요인으로 인해 자주 발생하고 있는 오 검지 상황들을 효과적으로 저감시킬 수 있는 능동적이고 환경적응적인 기법을 통해 사고 최종판단을 실시하였다. 이렇게 구현된 기술의 성능을 평가하고자 중부내륙 실험도로에서 12건의 사고 모의실험을 실시하였으며, 실제 운용되고 있는 장항IC에서의 사고영상을 실시간 온라인으로 입력받아 시험하였다. 결과적으로, 검지율 93.33%, 오검지 6.7%로 높은 신뢰성을 보였다.

"태양좌천(太陽左泉) 한음소승(寒淫所勝)의 병증(病證)과 기미(氣味)배합 분석" (Symptoms and Prescriptions Based on the Theory for Properties and Tastes of Korean Oriental Herbal Medicines with Regard to the Year When Taeyang is Affecting the Earth Energy and the Cold Energy is partially Over-abundant)

  • 양유인;서부일;신순식
    • 대한한의학방제학회지
    • /
    • 제12권2호
    • /
    • pp.19-30
    • /
    • 2004
  • This thesis aims to provide an analytical basis for existing or new prescriptions based on the theory for properties and tastes of Korean oriental herbal medicines. To this end, prescriptions presented in the Various Changes in the Dominations of the Six Energies and their Relations with the Diseases of Plain Questions were analyzed based on the theory for properties and tastes of Korean oriental herbal medicines in Yellow Emperor's Canon of Internal Medicine. This thesis focused on symptoms and prescriptions based on the theory for properties and tastes of Korean oriental herbal medicines, which were described in the Various Changes in the Dominations of the Six Energies and their Relations with the Diseases of Plain Questions with regard to the year when Taeyang is affecting the earth energy and the cold energy is partially over-abundant. Those symptoms and prescriptions originate from the theory of the five elements and six climates. The symptoms were analyzed from the perspective of physiology. The prescriptions were first analyzed based on the theory for properties and tastes of Korean oriental herbal medicines in Yellow Emperor's Canon of Internal Medicine. Then, a mix formula of oriental medicines pursuant to those prescriptions was studied. Lastly, established prescriptions, which were in conformity with the above prescriptions, were selected. From the physiological point of view, symptoms with regard to the year when Taeyang is affecting the earth energy and the cold energy is partially over-abundant can be, based on the theory of the five elements and six climates, diagnosed as the symptoms from impaired heart due to excessive cold energy. Established prescriptions pursuant to prescriptions based on the theory for properties and tastes of Korean oriental herbal medicines can be broken down into the following: Cassia Twig, White Peony and Anemarrhena Decoction (Gyejijakyakjimotang); Rhemannia Bolus with Eight Herbs (Palmijihoanghoan); Back to the Left Pill (Joaguihoan); Bone-Strengthening Pill (Hojamhoan); Major Eum-Replenishing Pill (Daeboeumhoan). Therefore, symptoms of six kinds of weather presented in the Various Changes in the Dominations of the Six Energies and their Relations with the Diseases of Plain Questions can be analyzed from the physiological point of view. As a result, in addition to the methodology that analyzes existing prescriptions within the boundary of the theory for properties and tastes of Korean oriental herbal medicines in Yellow Emperor's Canon of Internal Medicine, it is expected that a theoretical basis for new prescriptions can be provided by analyzing established prescriptions based on prescriptions from the theory for properties and tastes of Korean oriental herbal medicines.

  • PDF

미토콘드리아 호흡활성 전이온도의 측정에 의한 벼품종별 상대적 내냉성판정 (Transition Temperature of Mitochondrial Respiratory Activity as an Index for Chilling Susceptivity of Varieties of Rice Plant)

  • 정진;김희정
    • Applied Biological Chemistry
    • /
    • 제30권4호
    • /
    • pp.357-363
    • /
    • 1987
  • 생육기간동안 냉수처리하는 포장시험(작물시험장 시험사업)을 통해 재배학적방법으로 상대적 내냉성정도가 판정되어 있는 벼 41품종을 재배하여 그 유수로부터 분리한 미토콘드리아의 호흡활성전이 온도를 측정하였다. 강내냉성으로 분류된 품종들은 $11.5{\sim}13.5^{\circ}C$의 상대적으로 낮은 활성전이온도를 보였으며 내냉성이 약한 품종들일수록 그 온도가 높아졌다. 작물시험장의 내냉성 판정등급(강, 중강, 중, 중약 및 약)에 따라 그룹별로 해당품종들의 호흡활성 전이온도를 배열하면 그 순서는 그룹별로 내냉성이 약해지는 순서와 일치하였다. 이 결과는 유수미토콘드리아의 호흡활성 전이온도가 전반적 내냉성(overall cold-tolerance) 판정의 객관적 지표가 된다는 사실을 강하게 시사하고 있다. 호흡활성 전이온도의 간편한 측정을 위한 방법론을 분석생화학적 측면에서 검토하였다. 본연구에서 주로 이용한 polarographic method 이외에도 막에 결합된 cytochrome C oxidase가 호흡활성 및 그 전이온도를 결정한다는 발견에 입각하여 이 효소의 온도에 따른 활성변화를 spectrophotometric method로 측정하므로서 미토콘드리아 호흡계 전체의 활성전이온도를 측정할 수 있음도 확인하였다.

  • PDF