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Analysis of vitamin B12 in fresh cuts of Korean pork for update of national standard food composition table (국가표준식품성분표 개정을 위한 국내산 돈육의 부위별 비타민 B12 함량 분석)

  • Mun, Geum-Ju;Song, Wonju;Park, Sun-Hye;Jeong, Beom-Gyun;Jung, Gil-Rak;Choi, Kap Seong;Kim, Jin-Hyoung;Choi, Youngmin;Chun, Jiyeon
    • Food Science and Preservation
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    • v.24 no.7
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    • pp.983-991
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    • 2017
  • This study was performed to update the National Standard Food Composition Table (NSFCT) published by Korea Rural Development Administration, especially focusing on vitamin $B_{12}$ for Korean pork. Total 7 primal and 22 retail fresh cuts of Korean pork were analyzed for vitamin $B_{12}$ and the applied immunoaffinity-HPLC was validated. Vitamin $B_{12}$ assay by immunoaffinity-HPLC obtained recoveries over 95% and coefficient variations of precision below about 10%, which met the limits required for validation acceptance. Limits of detection and quantification of immunoaffinity-HPLC were 0.01 and $0.33{\mu}g/100g$, respectively. Quality control chart showed that analysis performance was excellent during the entire of study. Vitamin $B_{12}$ contents of pork cuts significantly varied depending the types of primal and its retail cuts (p<0.05). Belly, Boston butt, rib cuts showed relatively high vitamin $B_{12}$ contents compared to other primal cuts. Vitamin $B_{12}$ content of pork retail cuts were also significantly different within the same primal cuts (p<0.05). Among 22 retail cuts, the highest vitamin $B_{12}$ was observed in Tosisal in belly primal part ($0.98{\mu}g/100g$) while both Aldeungsimsal in loin and Hongdukkaesal in hide leg were the lowest by $0.33{\mu}g/100g$. This study provides reliable vitamin $B_{12}$ data for the Korean pork fresh cuts through standard sampling, method validation and analytical quality control, which would be used for update of Korean NSFCT.

Antioxidant activity and physicochemical composition of fermented Vigna angularis using Bacillus subtilis KCCM 11965P (Bacillus subtilis KCCM 11965P를 이용한 붉은팥 발효물의 항산화 활성 및 이화학성분 분석)

  • Jeong, Kyung Ok;Oh, Keun Su;Moon, Kwang Hyun;Kim, Dae Geun;Im, So Yeon;Lee, Eun Ji;Kim, Na Ri;Kim, Wook;Kim, Hae Jin;Lee, Jeong Ho
    • Food Science and Preservation
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    • v.24 no.7
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    • pp.975-982
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    • 2017
  • Health of human intestine has deteriorated due to excessive stress and western diet. In this study, Vigna angularis was fermented by Bacillus subtilis KCCM11965P in order to develop prebiotic resource for improving bowel movement. The contents of ash and crude protein were $3.35{\pm}0.04%$ and $21.1{\pm}0.19%$ respectively. Vigna angularis extract (1, 3, 5%) were incubated with 3% (v/v) Bacillus subtilis KCCM 11965P for 0, 24, 48, and 72 h. Total bacterial numbers showed that the combination of 3% powdered seeds and 72 h incubation time was optimum condition for this experiment. Total polyphenol content increased from $0.18{\pm}0.010mg/mL$ in pre-incubation to $0.23{\pm}0.007mg/mL$ in post-incubation with the condition mentioned above. DPPH radical scavenging activity also increased from $36.1{\pm}6.0%$ to $63.6{\pm}5.2%$. Analysis of protease activity showed $2.69{\pm}0.003unit/mL$ in combination of 5% powdered seeds and 72 h incubation time. Amylase activity increased from $1.0{\pm}0.1unit/mL$ in pre-incubation to $26.0{\pm}0.2unit/mL$ in post-incubation. The analysis of free amino acids after incubation with Bacillus subtilis KCCM 11965P showed that leucine increased from 5.22 mg/L to 67.59 mg/L and tyrosine, one of non-essential amino acid also increased 10.08 mg/L to 259.35 mg/L by incubation with 5% powdered seeds. Most of organic acid were reduced by incubation for 72 h. These results suggest that Vigna angularis could be utilized most as a prebiotic resources.

A Study on Job Stress and Job Satisfaction of Dental Hygienists Working in Dental Clinics (치과의원에 근무하는 치과위생사의 직무 스트레스와 직무만족에 관한 연구)

  • Jung, Jung-Ock;Song, Kwui-Sook
    • Journal of dental hygiene science
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    • v.8 no.4
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    • pp.305-312
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    • 2008
  • This study was attempted to look into relation between job stress and job satisfaction perceived by clinical dental hygienists and the influencing factors for providing fundamental data to increase their job satisfaction. For this, mailed correspondence examination was conducted with questionnaire for dental hygienists who are engaged in dental hygiene duties at dental clinics in Seoul between June 19 and July 24, 2008. Total 180 questionnaires were distributed, and among these, 156 questionnaires were included in analysis except for 24 questionnaires having incomplete answers. The findings of this study are as follows: 1. The total mean of dental hygienists' job stress was found to be 3.46 points out of 5 points, and that of their job satisfaction to be 2.99. 2. For relation between general characteristics and job stress of dental hygienists, significant difference was found just in the motive to select dental hygienist (p < 0.001) and the expected period of engagement in dental hygienist duties (p < 0.05). 3. For relation between general characteristics and job satisfaction of dental hygienists, significant difference was found just in job experience(p < 0.05), current position (p < 0.05), average salary (p < 0.001), and the expected period of engagement in dental hygienist duties (p < 0.01). 4. For correlation between job stress and job satisfaction, negative correlation was shown generally (r=-0.222), such that the more was job stress, the less was job satisfaction. 5. As a result of multiple regression analysis to look into the job stress factors influencing dental hygienists' job satisfaction, it was found that the factors of excessive workload(p < 0.05) and improper treatment(p < 0.01) most influenced job satisfaction, and the descriptive power on the job stress factors influencing their job satisfaction was found to be 24.1%.

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Development of Evaluation Index for Infection Control and Prevention at Dental Hospital and Its Validity Verification (치과의료기관 감염관리 평가지표 개발 및 타당성 검증)

  • Bae, Sung-Suk;Lee, Myung-Sun
    • Journal of dental hygiene science
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    • v.13 no.3
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    • pp.254-263
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    • 2013
  • This study is to develop an evaluation index for infection control and to verify its validity by examining each set of weighted data collected from 121 infection control personnel at dental hospitals who agreed to the preliminary survey and advisory. The study was conducted from 14th December, 2010 to 31st January, 2011, and PASW Statistics 18.0 and AMOS 5.0 had been used for the statistical data analysis. As a result of the study, five evaluation factors with 21 sub-indicators have been identified at structural level, eight evaluation factors with 32 sub-indicators at processing level, and one evaluation fact with five sub-indicators at resulting level, total 14 evaluation factors with 58 sub-indicators throughout all levels. The path analysis added on the result that 'standard precautions ($x_1$)', 'infection control support system ($x_2$)', 'internal and external characteristics ($x_3$)' are exogenous variables that affect on other variables, and 'standard infection control ($y_1$)','Organization equipment management handwashing ($y_2$)', 'environmental infection control ($y_3$), 'personal protective equipment ($y_4$)', 'waste and laundry management ($y_5$)' are endogenous variables that are infulenced by others. The standardized metrics are more needed than anything else when examining on infection control. This study attempts to develop proper dental infection control metrics adequately adjusted for domestic circumstances, and therefore to contribute to effective systematic management and decision-making in infection control.

The Analysis of Operational Characteristics in Contract - managed Highschool Foodservice in Seoul (서울시 소재 고등학교 위탁급식 운영현황 분석)

  • Yang, Il-Seon;Kim, Hyeon-A;Sin, Seo-Yeong;Jo, Mi-Na;Park, Su-Yeon;Cha, Jin-A;Lee, Bo-Suk
    • Journal of the Korean Dietetic Association
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    • v.8 no.3
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    • pp.280-288
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    • 2002
  • The purpose of this study was to investigate the operational characteristics of the contract-managed highschool foodservice and to analyze the factors to effect the menu price. The data was collected from 249 highschools in Seoul. The results are as follows; Those surveyed highschools were established as 1 national, 74 public, and 174 private institution. Highschools were classified as 176 academic, 66 vocational, and 7 specific purposed institution. Students were organized as 70 boys', 23 girls', and 41 coeducational highschools. Most highschool started contract-managed highschool foodservice from 1999 and the period of foodservice contract was most 3 years and the operation styles in food distribution were 96 classrooms, 105 dining halls and 17 classrooms combined dining halls. The scale of contract foodservice management companies was 63.1% small and medium and 36.9% large enterprises. The surveyed highschools had the average meal price 2,141 won per meal and they had 1,518 pupils on the register. The participating rate to the foodservice was 68.5%. The facilities investment cost of the contract foodservice management company was 179,204,230 won for private institutions and was 138,119,010 won for national&public institutions. The period of the contract was 3.22 years in private institutions, which was significantly higher than national&public institutions which showed 2.85 years. The commissary foodservice schools had higher facilities investment cost than conventional foodservice schools. Classrooms foodservice had higher participating foodservice rate than Dining halls. The investment cost for facilities showed high in order of girls', boys', and coeducational high schools, and the number on the register and the number participating in the foodservice showed high in order of boys', girls', and coeducational high schools. The number on the register showed the highest in academic and vocational schools, specific purposed institutions in sequence, and the number participating in the foodservice showed high in order of academic schools, specific purposed institutions and vocational. However, the participating foodservice rate showed high in specific purposed institution, academic and vocational schools in order, and the meal price, the investment cost for facilities showed high in specific purposed institution, academic and vocational schools in sequence. Regionally, the district south of Han river had the average meal price 2,266.13 won, which showed higher in the eastern part which had 2,033.33 won. The western part had the average investment cost for facilities of 233,331,060 won, and the central district 126,137,140 won. The number on the register showed 1845.68 in the eastern part and 1308.00 in Dong-Jak area, that had clear differences among areas. When the period of the contract went longer, the investment cost for facilities had a tendency to increase. The significant differences were existed among meal price, the investment cost for facilities, the number on the register, the number participating in the foodservice, and the participating foodservice rate. The investment cost for facilities had increased according to the number participating in the foodservice and the participating foodservice rate. And the large enterprises showed higher participating foodservice rate than the small and medium enterprises.

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Analyzing the Importance and Performance of Sanitation Management within Childcare Center Foodservice Facilities in Gyeongbuk Province (경북 지역 보육 시설 급식소의 위생 관리에 대한 중요도-수행도 조사)

  • Jung, Hyeon-A;Kim, An-Na;Joo, Na-Mi;Paik, Jae-Eun
    • Journal of the East Asian Society of Dietary Life
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    • v.21 no.3
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    • pp.385-391
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    • 2011
  • The purpose of this study was to analyze the importance and performance of sanitation management within childcare center foodservice facilities in Gyeongbuk province. The survey involved 248 people who participated in the food hygiene and safety education for childcare center managers in Gyeongbuk province from June to July 2010. A total of 236 survey papers were analyzed statistically by SPSS program. By the method, t-test and importance-performance analysis (IPA) was performed. Among the respondents, facility managers are 40s (41.9%) which accounted the most percentage, and for the type of facility, the largest amount was home (39.9%). The number of preschool children who are under 20 people accounted for 45.3% and food service staffs were accounted for 36.7%. No significant differences were observed for the four items on the IPA, except for the topics "the immediate deal with trash and leftover food" and "minimizing time (within 2 hours) for distribution after cooking" (p<0.001). The average score on the IPA was 4.14 points, and the average score for the importance of the evaluation was 4.49 points out of 5. Finally, childcare facility managers are aware of the importance that is lower than can be done. The results suggest that continuing education is necessary to administrators to manage the health care facility effectively.

Distribution Dynamics of Fish Community in Shallow Wetland by Environmental Variables (얕은 습지에서 환경 요인에 따른 어류상 분포 특성)

  • Choi, Jong-Yun;Jo, Hyunbin;Kim, Seong-Ki;La, Geung-Hwan;Joo, Gea-Jae
    • Korean Journal of Environment and Ecology
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    • v.29 no.3
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    • pp.391-400
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    • 2015
  • In order to investigate the distribution and species composition of fish in shallow wetlands that might be affected by environmental factors, we investigated the physicochemical parameters, macrophytes biomass, and fish assemblage in 24 shallow wetlands in South Korea from May to June, 2012. In this study, a total of 20 fish species were identified, and Cypinidae were found to be the most dominant species. Physicochemical parameters and macrophyte biomass were different in the survey sites, and macrophytes biomass, in particular, showed a positive relationship with fish abundance in stepwise multiple regression (df=1, F=32.00, P=0.001). According to the result of the cluster analysis between survey sites, the survey sites were divided into three groups in accordance with species composition of fish in relation to macrophytes biomass. In the wetlands of the first group, Lepomis macrochirus which belongs to Centrarchidae was found to be dominant and other fish assemblages were hardly seen. In the second group, unlike the first group, Carassius auratus that belongs to Cypinidae was found to be dominant. In the third group, Lepomis macrochirus was found to be as dominant as the first group but various other fish species appeared. Where there was abundance of the main food sources (i. e. zooplankton) of fish in the survey sites, there were more diverse macrophyte biomass. Consequently, it is proven that macrophytes strongly affect the species composition and abundance of fish, and high biomass of macrophytes support high assemblage of fish. Based on these results, we recommend establishing diverse aquatic macrophytes communities when restoring or creating wetlands to assure high diversity of fish species that use macrophytes as their habitat.

Development of Rubber Damper of Flywheel for Diesel Engine (디젤기관(機關) 플라이휠의 고무댐퍼 개발(開發))

  • Myung, Byung Soo;Kim, Sung Rai
    • Korean Journal of Agricultural Science
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    • v.20 no.1
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    • pp.68-87
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    • 1993
  • Data acquisition system and computer program developed in this study could be well used in engine vibration analysis. The system and program developed were also operated to be able to control measuring interval, number of channels, number of data. The flywheel was specially studied to provide the proper weight with rubber damper for the engine design at low level of vibration. This study was conducted to obtain basic data which affect the engine vibration. The experiment of this study was performed on original weight flywheel, weight-reduced flywheel, weight-reduced and rubber-coated flywheel, weight-reduced and damper-attached flywheel. Avarage of peak value, maximum vibration, power spectrum density based on FFT analysis are major factors of this experiment. Results were obtained as follows : 1. When rubber was inserted in the flywheel rim of which weight was reduced from 32.2kgf to 24.4kgf, maximum vibration of the engine was decreased 48.3% at X axis, 35.5% at Y axis and 34.6% at Z axis in comparison with the flywheel of original weight. 2. When the flywheel of rubber damper was compared with the original flywheel, the average of absolute vibration for rubber damped flywheel was decreased at X, Y, Z axis and especially its decreasing rate was so high at X-axis comparing with the other flywheel, which implied that rubber damper was very useful to reducing the vibration of the engine at X axis. 3. Hysteresis losses of X, Y, Z axis were greatly decreased in the flywheel with rubber damper on rim. 4. Damped oscillation effect on X and Y axis vibration above average peak vibration by the flywheel of rubber damper on rim was larger than those by the other flywheels. 5. Power spectrums of vibration at real and imaginery part were bi-mode type. The vibration frequency of rubber dampered flywheel which weight is decreased was slightly increased as compared with original flywheel.

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A Study on Factors Affecting Emotional Labor of Clinical Dental Hygienists (임상치과위생사들의 감정노동에 영향을 미치는 요인)

  • Lee, Myeong-Ju;Kim, Young Nam;Lee, So-Young
    • Journal of dental hygiene science
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    • v.15 no.2
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    • pp.113-118
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    • 2015
  • This study was conducted to develop a plan for emotional labor of clinical dental hygienists by understanding the factors affecting their emotional labor. Self-administered questionnaires were distributed to 293 dental hygienists who worked in dental hospitals and clinics located in Busan and Gyeongsangnam-do from June to September in 2013. The questionnaire items used in this study consisted of 5 items of general characteristics, 2 items of characteristics related to turnover, and 9 items of emotional labor. t-test, ANOVA, and multiple regression analysis were performed using the SPSS Statistics ver. 17.0 for Windows program for data analysis. The average level of the study subjects was $69.85{\pm}12.82$. With regard to the emotional labor according to the general characteristics, the frequency of emotional expressions (p<0.037) showed significant differences in the marital status. The workplace showed significant differences in the frequency of emotional expressions (p<0.001), attentiveness of emotional expressions (p<0.002), emotional dissonance (p<0.032), and total (p<0.001). The turnover intentions showed significant differences in the frequency of emotional expressions (p<0.001), attentiveness of emotional expressions (p<0.001), emotional dissonance (p<0.001), and total (p<0.001). As a factor affecting emotional labor, turnover intention (p<0.001) and workplace (p<0.001) had an effect on emotional labor. The results showed that turnover intention and workplace were associated with the emotional labor of dental hygienists. There is a need to develop an emotional education program to make it possible to effectively manage emotional labor of dental hygienists for providing high-quality medical services of dental institutions.

Single Nucleotide Polymorphisms (SNPs) Discovery in GHSR Gene and Their Association Analysis with Economic Traits in Korean Native Chickens (GHSR 유전자 내 유전변이의 탐색과 한국재래계의 성장 및 산란 특성에 미치는 연관성 분석)

  • Choi, So-Young;Hong, Min-Wook;Yang, Song-Yi;Kim, Chong-Dae;Jeong, Dong Kee;Hong, Yeong Ho;Lee, Sung-Jin
    • Korean Journal of Poultry Science
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    • v.43 no.4
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    • pp.273-279
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    • 2016
  • Recently, it was reported that certain polymorphisms in the growth hormone secretagogue receptor gene (GHSR) are associated with the growth of chickens. However, the correlation between GHSR polymorphisms and economic traits has not been investigated in Korean native chickens (KNCs). Therefore, the objective of this study was to confirm the suitability of the GHSR gene as a candidate for genomic selection and identify a genetic marker for KNCs. A total of 220 KNCs from six breeds raised at the National Institute of Animal Science were genotyped for the c.739+726 SNP in the GHSR gene using polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP), and the sequence for a subset of 30 birds was analyzed using direct sequencing. The association between the SNP genotypes and the economic traits of the KNCs was analyzed using the statistical package for the social science (SPSS) software program. The association analysis between the c.739+726T>C SNP and economic traits revealed that the SNP was significantly associated with body weight at 150 and 270 days (BW150 and BW270, respectively) in all KNCs (p<0.01), BW150 in KNC (Gary) (p<0.05), and egg production number in KNC (White, p<0.05). In addition, the SNPs discovered using direct sequencing (513A>G, 517A>T) had a significant effect on the body weight and egg production traits (p<0.05). In conclusion, these results might be useful as a basis for studies on the improvement of KNC breeds. Furthermore, these results suggest that the SNPs (c.739+726T>C, 513A>G, and 517A>T) located in the GHSR gene could be useful molecular genetic markers for KNCs.