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http://dx.doi.org/10.5536/KJPS.2016.43.4.273

Single Nucleotide Polymorphisms (SNPs) Discovery in GHSR Gene and Their Association Analysis with Economic Traits in Korean Native Chickens  

Choi, So-Young (College of Animal Life Sciences, Kangwon National University)
Hong, Min-Wook (College of Animal Life Sciences, Kangwon National University)
Yang, Song-Yi (College of Animal Life Sciences, Kangwon National University)
Kim, Chong-Dae (Poultry Science Division, National Institute of Animal Science, RDA)
Jeong, Dong Kee (Department of Animal Biotechnology, Jeju National University)
Hong, Yeong Ho (Department of Animal Science and Technology, Chung-Ang University)
Lee, Sung-Jin (College of Animal Life Sciences, Kangwon National University)
Publication Information
Korean Journal of Poultry Science / v.43, no.4, 2016 , pp. 273-279 More about this Journal
Abstract
Recently, it was reported that certain polymorphisms in the growth hormone secretagogue receptor gene (GHSR) are associated with the growth of chickens. However, the correlation between GHSR polymorphisms and economic traits has not been investigated in Korean native chickens (KNCs). Therefore, the objective of this study was to confirm the suitability of the GHSR gene as a candidate for genomic selection and identify a genetic marker for KNCs. A total of 220 KNCs from six breeds raised at the National Institute of Animal Science were genotyped for the c.739+726 SNP in the GHSR gene using polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP), and the sequence for a subset of 30 birds was analyzed using direct sequencing. The association between the SNP genotypes and the economic traits of the KNCs was analyzed using the statistical package for the social science (SPSS) software program. The association analysis between the c.739+726T>C SNP and economic traits revealed that the SNP was significantly associated with body weight at 150 and 270 days (BW150 and BW270, respectively) in all KNCs (p<0.01), BW150 in KNC (Gary) (p<0.05), and egg production number in KNC (White, p<0.05). In addition, the SNPs discovered using direct sequencing (513A>G, 517A>T) had a significant effect on the body weight and egg production traits (p<0.05). In conclusion, these results might be useful as a basis for studies on the improvement of KNC breeds. Furthermore, these results suggest that the SNPs (c.739+726T>C, 513A>G, and 517A>T) located in the GHSR gene could be useful molecular genetic markers for KNCs.
Keywords
GHSR; Korean native chickens; SNP; economic traits;
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Times Cited By KSCI : 2  (Citation Analysis)
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