• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2003.09a
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• pp.368-371
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• 2003

Accidental release of petroleum products from underground storage tank(USTs) is one of the most common causes of groundwater contamination. BTEX is the major components of fuel oils, which are hazardous substances regulated by many nations. In addition to BTEX, other gasoline consituents such as MTBE(methyl-t-buthyl ether), anphthalene are also toxic to humans. Natual attenuation processes include physic, chemical, and biological trasformation. Aerobic and anaerobic biodegradation are believed to be the major processes that account for both containment of the petroleum-hydrocarbon plum and reduction of the contaminant concentrations. Aerobic bioremediation has been highly effective in the remediation of many fuel releases. However, Bioremediation of aromatic hydrocarbons in groundwater and sediments is ofen limited by the inability to provide sufficient oxygen to the contaminated zones due to the low water solubility of oxygen. Anaerobic processes refer to a variety of biodegradation mechanisms that use nitrate, ferric iron, sulfate, and carbon dioxide as terminal electron accepters. The objectives of this study was to conduct laboratory-scale microcosm studies in assessing enhanced bioremediation potential of BTEX and MTBE under various electron accepters(aerobic, nitrate, ferric iron, sulfate) in contaminated Soil. these results suggest that, presents evidence and a variety pattern of the biological removal of aromatic compounds under enhanced nitrate-, Fe(III)-, sulfate-reducing conditions.

• Journal of Korean Society of Water and Wastewater
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• v.25 no.4
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• pp.611-616
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• 2011

The impacts of ultrasonic pretreatment on the biodegradability of domestic sewage sludge were evaluated through a series of anaerobic digestion experiments in batch system. The gas and methane production from the sludge samples pretreated by an ultrasonic tool with different durations were measured with time. Although the biogas production increased with the extent of sludge solubilization and the period of ultrasonic pretreatment, the enhancement of sludge biodegradability was much more sensitive to the pretreatment for the relatively short periods. Most of the enhanced biodegradability by the pretreatment was appeared in the early stage of anaerobic digestion, less than 6 days. The maximum biogas production per day was observed between 4 to 6 days when the sludge was pretreated less than 10 minutes while it was obtained in the beginning for the sludge pretreated longer periods. The results suggest that the repeated alternation of low strength ultrasonic pretreatment and anaerobic digestion may be more effective than the combination of one time pretreatment for a relatively long period and following anaerobic digestion.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2004.04a
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• pp.131-134
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• 2004

Several physical and chemical methods have been used for remediation contaminated by oils. However the cost was very high and secondary pollution rose during treating. The purpose of this study was to comprision TPH (total petroleum hydrocarbon) removal from artificially contaminated soil by diesel with and without seeding anaerobic digested sludge. After 120 days of overall at 35$^{\circ}C$, removal efficiency of TPH with seeding sludge was 2-3 times higher than blank. Also, the more amount seeding sludge, TPH removal efficiency and CH$_4$ content more obtained. It was sad that seeding of anaerobic digested sludge was a good method for enhancing TPH removal efficiency without increasing operating cost. Sulfate, nitrate-reducing, methanogenic condition were evaluated for alkane, isoprenoid as target contaminated soil.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2003.04a
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• pp.368-371
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• 2003

Leaking underground storage tanks are a major source of groundwater contamination by petroleum hydrocarbons. Bioremediation of aromatic hydrocarbons in groundwater and sediments is often limited by dissolved oxygen. Aerobic bioremediation has been highly effective in the remediation of many fuel releases, but Many aromatic hydrocarbons degrade very slowly under anaerobic conditions. Nitrate is a good alternative electron acceptor to oxygen and denitrifying bacteria are commonly found in the subsurface and in association with contaminated aquifer materials. Because nitrate is less expensive and more soluble than oxygen. it may be more economical to restore fuel-contaminated aquifers using nitrate rather than oxygen. This study show that biodegradation of BTEX and MTBE is enhanced by the nitrate-amended microcosms under aerobic/anaerobic conditons. Although aromatic hydrocarbons degrade very slowly under anaerobic conditions. Biodegradaton was observed for all of the test compounds.

• Korean Journal of Environmental Agriculture
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• v.23 no.3
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• pp.138-141
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• 2004

Seven soil samples were collected from paddy fields located nearby Nagoya city in Japan. All the soils were subjected to flooded condition and incubated with PCP at $30^{\circ}C$ for two months, and their anaerobic PCP degradation have been monitored by checking the PCP concentration of the soils at regular intervals. The degradation of PCP did not occur in the soils autoclaved two times before pre-incubation. On the other hand, all the soils showed significant PCP degradation in non-sterilized condition after 30 days of incubation, except far one soil sample (Yatomi), in which PCP was rarely degraded until 30 days of incubation. This result showed PCP disappearance in the pad(rf soils was mainly caused by microbiological activity, and depended upon the physicochemical characteristics of the soils.

• Journal of Environmental Health Sciences
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• v.22 no.2
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• pp.96-103
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• 1996

The objectives of this study were to examine the biodegradation of phenol using the anaerobic fluidized bed reactor(AFBR). Mixed microorganisms were selected from the anaerobic digestion tank, and could be adapted to high concentration of phenol by increasing the phenol concentration 600-3600 mg/l step by step. The results were summarized as follows: 1. The average removal efficiency of phenol was 90%, decreased by increasing concentration of phenol, and then a shock range was 1200~2400 ppm. 2. The production rate of biogas in overall limits was proportional to the concentration of influent phenol. 3. At steady state, compositions of gases were $CH_4$ 55~60%, $C0_2$ 34~43%, respectively. These were similar to that of the theoretical estimates. 4. The production rates of biogas and methane per the molarity of phenol removed were linearly increased, 56.45 l gas/mol-phenol and 29.20 l $CH_4/mol$-phenol. Using this biogas, the recoverable energy was 269.1 kcal/mol phenol. It was 120.2 kcal/g-COD, transforming into the chemical oxygen demand. 5. The bulk of microorganisms existed in suspended section of fluidized bed with type of biofilm and its concentration was 340 mg/g-media. In conclusion, the anaerobic treatment of pure phenol was possible and its removal efficiency, introducing the AFBR, was successful. Also toxic organic compound such as phenol was biodegradable and was recoverable as resource of energy.

• Journal of Environmental Science International
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• v.15 no.9
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• pp.897-905
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• 2006

This study has been conducted to investigate biodegradation characteristics and optimum mixing ratio for co-digestion with thermophilic acid-fermented food waste and sewage sludge using batch anaerobic digester. As the basis operating conditions for anaerobic digestion, the reaction temperature was controlled $35{\pm}1^{\circ}C$ and stirrer was set 70rpm. Thermophilic acid-fermented food waste and sewage sludge were mixed at the ratio of 10:0, 7:3, 5:5, 3:7, 0:10 and 5;5(food waste : sewage sludge) as the influent substrates. In results of co-digestion according to mixing ratio of thermophilic fermented food wastes and sewage sludge in batch mesophilic anaerobic digestion reactor, $385mL\;CH_4/g\;VS_{added}$ of methane production rate at 1:1 mixing ratio was more than that of any other mixing ratios. Compared with $293mL\;CH_4/g\;VS_{added}$ of methane production rate at 1:1 mixing ratio of food wastes and sewage sludge, pretreatment of food wastes by thermophilic acid fermentation was more effective in co-digestion with sewage sludge.

• Journal of the Korean GEO-environmental Society
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• v.15 no.5
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• pp.39-46
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• 2014

The objectives of this study were to determine biodegradation and characteristics of BTEX and MTBE under aerovic-anaerobic conditions and evaluate the potential of natural attenuation method in denitrifying condition.. In the single-substrate experiments, all of the BTEX compounds were degraded under all the conditions. but, lower degradation of benzene and p-xylene were observed under aerobic condition due to the lack of oxygen initially supplied. In the mixed-substrate experiments, BTEX degradation was delayed compared to that in the single-substrate experiments due to a competition of the substrates. Biodegradation of MTBE was observed only under denitrifying conditions and we expected that MTBE mineralized to $CO_2$ without the accumulation of TBA. We also conducted to determine the effect of initial nitrate concentration on BTEX and MTBE degradation. At low nitrate concentration (<50 mg/L), BTEX degradations were limited by the lack of electron acceptor and BTEX degradation was inhibited at high nitrate concentration (>200 mg/L). The results in this study indicated that biotransformation could be applied to the gasoline-contaminated region under aerovic-anaerobic.

• Journal of Microbiology and Biotechnology
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• v.30 no.6
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• pp.839-847
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• 2020

In the present study, an anaerobic microbial consortium for the degradation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) was selectively enriched with the co-addition of RDX and starch under nitrogen-deficient conditions. Microbial growth and anaerobic RDX biodegradation were effectively enhanced by the co-addition of RDX and starch, which resulted in increased RDX biotransformation to nitroso derivatives at a greater specific degradation rate than those for previously reported anaerobic RDX-degrading bacteria (isolates). The accumulation of the most toxic RDX degradation intermediate (MNX [hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine]) was significantly reduced by starch addition, suggesting improved RDX detoxification by the co-addition of RDX and starch. The subsequent MiSeq sequencing that targeted the bacterial 16S rRNA gene revealed that the Sporolactobacillus, Clostridium, and Paenibacillus populations were involved in the enhanced anaerobic RDX degradation. These results suggest that these three bacterial populations are important for anaerobic RDX degradation and detoxification. The findings from this work imply that the Sporolactobacillus, Clostridium, and Paenibacillus dominant microbial consortium may be valuable for the development of bioremediation resources for RDX-contaminated environments.

• Journal of the Korea Organic Resources Recycling Association
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• v.16 no.3
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• pp.83-90
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• 2008

The ultrasonic pre-treatment of sewage sludge (SS) was investigated to increase soluble organic material and to improve anaerobic biodegradability. Ultrasonic disintegration of SS increased the amount of soluble chemical oxygen demand (SCOD), protein and carbohydrate concentrations whereas particle size decreased due to the break-up of cell walls. In terms of anaerobic biodegradability, ultrasonic pre-treatment enhanced the anaerobic biodegradation of SS, leading to the methane gas production improvement. Biochemical methane potential (BMP) of SS was 211.3 ml $CH_4/gVS$ whereas BMP after ultrasonic pre-treatment was 294.3 ml $CH_4/gVS$. The improvement in BMP for SS treated with ultrasonic disintegration was as high as 40 %. This result indicated that disintegration of SS was efficient for enhancing anaerobic biodegradability.