• KSBB Journal
• /
• 제9권2호
• /
• pp.165-173
• /
• 1994

재조합 소성장호르몬을 트립신, S.aureus V8 단백질가수분해효소, CNBr, 그리고 산 가수분해법을 이용하여 단백질 일차구조 분석을 실시하였다. N-말단 분석은 30 잔기까지를 수행하였는데, 대장균 내에서 발현된 소성장호르몬은 E. coli 내 에 존재 하는 methionyl-aminopeptidase에 의해 해독개시인자로 넣어준 N-말단의 Met이 모두 제거된 형태로 나타났으며 아미노산 조성분석 결과 연역된 조성과 유사하게 나타났다. 효소와 화학물질로 절단한 소성장호르몬 조각들을 HPLC로 분리한 후 단백질 서열분석기를 이용하여 아미노산 서열을 분석하였다. 대장균에서 발현된 소성장호르몬은 191개의 아미노산으로 구성된 21,802 Da의 분자량을 갖고 있는 단백질로 나타났다. 여기에서 을 갖고 있는 단백질로 나타났다. 여기에서 얻은 아미노산 서열을 바탕으로 hydropathy plot을 한 결과 N-말단에서는 소수성이 그리고 C-말단에서는 친수성 영역이 나타났다.

• KSBB Journal
• /
• 제10권3호
• /
• pp.271-282
• /
• 1995

met-hGH로부터 고정화 ApM에 의해 전환된 천 연형 hGH를 등전위점, 소수성, 전하량의 차이에 따라 3회의 일련의 크로마토그래피를 수행하여 정제하고, 정제된 hGH의 특성을 조사하였다. 정제한 hGH의 비활성은 효소면역측정법으로 분석하였을 때 2 2.75IUjmg이었고, hGH 회수율은 14.1%이었다. 정제한 재조합 hGH는 아미노산 조성, 아미노 말단의 15개의 아미노산 서열 분석, 카르복시 말단의 아미노산 분석, 트럽신 가수분해 랩타이드들의 HPLC 분석등을 통해 아미노말단 에치오닌이 제거된 천연 형 hGH임을 알 수 있었고, SDS-PAGE, native­P PAGE, IEF, RP-HPLC, HSGF 등의 분석 결과 정제한 hGH는 상용품보다 순도가 높다는 것을 알 수 있었으며, 뇌하수체 제거 쥐의 체중 증가와 경골성 장폭의 증가를 통한 활성 측정 결과 정제한 hGH는 상용 hGH와 같은 정도의 활성을 냐타내었으며 우혈청 알부민을 주사한 대조군에 비해 뚜렷한 성장 효 과를 나타냈다. 따라서 본 연구에셔 정제한 hGH는 천연형이며, 높은 순도와 우수한 생리활성을 나타내는 것을 알 수 있었다.

• Toxicological Research
• /
• 제12권2호
• /
• pp.277-281
• /
• 1996

To evaluate the renal toxicity of the antitumor agent, 5-(piperidonomethylphenyl)-2,3-dihydroimidazo[2,1-a]isoquinoline (SDZ-62-434), rats were treated with SDZ-62-434 of 50 mg/Kg, i.p., once and 10 mg/Kg, i.p., daily for 7 days. The kidney weights and urine volume after and during the treatment were observed. The concentrations of urinary creatinine, protein, and the activities of N-acetyl-$\beta $D-glucosaminidase (NAG), alanine aminopeptidase (AAP), $\gamma$-glutamyl transpeptidase (GGT) and lactate dehydrogenase (LDH) in 24 hr urine were also determined. The kidney weights after acute and subacute administration was not affected. The urine excretions were increased 5 days after the acute administration and increased after the daily 3rd day-administration. The excretion of creatinine was similar as that of urine excretion. The excretion of creatinine was increased 5 days after the acute and subacute administration. However, the protein excretion didn't changed in both treatment. Those indicate that SDZ-62-434 might induce the diuresis and also suggest that diuresis might be due to the some metabolites rather than the compound itself. The urinary activities of NAG and LDH were not affected after the acute treatment. However, the urinary activities of AAP and GGT were slightly increased 3 days after the acute administration but, returned to the control value. In subacute treatment, the activities of GGT was not changed. And the activities of NAG were declined after the 7th day-administration. However, the activities of AAP were significantly increased after the 5th day-administration. Furthermore, the urinary activities of LDH were continuously increased during the subacute administration. These results indicate that the high and subacute administration might induce a weak damage on the kidney cells. Furtherrnore, the present results suggest that SDZ-62-434 might have relatively slow-emerging and mild toxicity to the kidney.

• 한국산림과학회지
• /
• 제58권1호
• /
• pp.1-7
• /
• 1982

소나무의 glutamate-oxalate transaminase(GOT)와 leucine aminopeptidase(LAP)의 유전양식(遺傳樣式) 동위효소(同位酵素) 유전자좌(遺傳子座) 간의 연관관계(連關關係)의 구명을 목적으로 배유(胚乳) 및 배조직(胚組織)을 감자전분 전기영동법에 의하여 분석(分析)하였다. 4개의 GOT 지역(地域)(GOT-A~GOT-D)이 분리(分離)되었으며 가장 이동(移動)이 빠른 GOT-A 지역(地域)에서는 두개의 밴드를 보이는 표현형(表現型)으로 변이(變異)가 발견(發見)되지 않았다. GOT-B와 GOT-C 두 지역(地域)의 동위효소(同位酵素) 표현형(表現型)들을 공히 1:1의 분리비(分離比)를 보여 각각 하나의 유전자좌(遺傳子座)에 의해 지배(支配)됨이 추정(推定)되었으며 양유전자좌(兩遺傳子座)에서 각각 4개와 3개의 대립(對立) 유전자(遺傳子)가 확인되었다. 음극(陰極)으로 이동(移動)한 GOT-D 지역(地域)의 표현형(表現型)은 GOT-C 지역(地域)과 동일하게 분리(分離)되었다. 이 두 지역(地域)이 한 개의 동일한 유전자좌(遺傳子座)의 지배(支配)를 받는지 또는 강하게 연관(連關)되어 있는 두 개의 유전자좌(遺傳子座)에 의한 것인지 단정할 수 없었다. 변이(變異)를 보인 세 개의 GOT 지역(地域)에서 이형접합성(異形接合性)의 배조직(胚組織)은 세 개의 밴드를 보이는 표현형(表現型)을 보여 소나무의 GOT 동위효소(同位酵素)가 이량체(二量體)임을 제시하였다. LAP 동위효소(同位酵素)는 2개의 지역(地域)으로 분리(分離)되었다. 각 지역(地域)의 동위효소(同位酵素) 표현형(表現型)은 각각 1:1의 분리비(分離比)를 보여 LAP 동위효소(同位酵素)가 2개의 유전자좌(遺傳子座)에 의해 지배(支配)됨이 확인되었고 두 유전자좌(遺傳子座)에서 2개와 3개의 대립유전자(對立遺傳子)가 각각 확인되었다. GOT-B와 LAP-B는 강하게 연관(連關)되어 있음이 밝혀졌고, 이들 간의 평균(平均) 재조합(再組合) 비율(比率)은 12.5%였다. 또 다른 한쌍의 유전자좌(遺傳子座), GOT-B와 GOT-C 간에도 재조합(再組合) 비율(比率)이 41%로 나타나 연관(連關)의 가능성(可能性)이 제시되었다.

• 한국균학회지
• /
• 제27권5호
• /
• pp.328-336
• /
• 1999

Isozyme comparisons of mycelial extracts from Pleurotus ostreatus were undertaken using isoelectric focusing. Enzyme isozyme patterns were Used to describe the extent of geographical diversity and degree of intraspecific variation in these extracts. A total of 77 bands were resolved from six different enzymes. Cluster analyses were performed using the zymograms for esterase (EST), glucose phosphate isomerase (GPI), leucine aminopeptidase (LAP), malate dehydrogenase(MDH), peroxidase (POX), and phosphoglucomutase (pGM). EST gave multiple banding patterns, while less variability was observed for GPI, MDH, and PGM. Cluster analyses demonstrated that strains of P. ostreatus from geographically different origins are genetically divergent, supporting the idea that there is little or no gene flow between these geographically distant population groups.

• Ocean and Polar Research
• /
• 제24권1호
• /
• pp.47-53
• /
• 2002

To evaluate the effect of reed population on the distribution and activities of microorganisms, vertical distribution of heterotrophic bacteria, degradation rate of cellulose, extracellular aminopeptidase activity (APA) and metabolic diversity based on GN2 Microlog plate were measured at two salt marsh stations in Hogok-ri, Namyang Bay, west coast of Korea. The number of heterotrophic bacteria at station 1 (reed population inhabited area) showed 2 to 6 times higher than that of station 2 (exposed area) with exception in the surface layer. Cellulose degradation rates in station 1 showed more than 50%. month-I and higher than that of station 2 (10.2 to 38.4%. $month^{-1}$). Yet the APA at two stations did not show difference except surface layer and suggested that APA might not be a significant factor in degrading marsh plant debris. Lipid class compounds, cell wall polymers and L-alanine were widely used by microorganisms. The number and activities of bacterial populations especially concerned in plant debris degradation seemed to be stimulated by the reed communities.

• 대한약학회:학술대회논문집
• /
• 대한약학회 2001년도 Proceedings of the Pharmaceutical Society of Korea
• /
• pp.90-94
• /
• 2001

Wondonins A and B, aromatic alkaloids of an unprecedented skeletal class have been isolated form and association of the sponges Poecillastra wondoensis and Jaspis sp. In addition, four novel bromotyrosine-derived metabolites, psammaplins $A_1$ and $A_2$, aplysinellins A and B, have been isolated from the tropical sponge Aplysinella rhax. The structures of these compounds have been determined on the basis of combined chemical and spectral analyses. The new compounds exhibited significant cytotoxicity and antiangiogenic activity as well as inhibitory activities against farnesyl protein transferase and leucine aminopeptidase. In addition to these compounds, several bioactive metabolites have been isolated from sponges of Korean and tropical waters.

• 한국약용작물학회:학술대회논문집
• /
• 한국약용작물학회 2006년도 Spring Conference
• /
• pp.75-90
• /
• 2006

Both synthetic and endogenous $A{\beta}$ are degraded by peptidase G. Both $A{\beta}40$ and 42 are cleaved by peptidase G. Peptidase G cleaves $A{\beta}40$ into small fragments ($A{\beta}18$) which lacks aggregation property and are not toxic to neuron. Peptidase G seems to degrade multimeric $A{\beta}$ more efficiently than monomeric $A{\beta}$. Peptidase G protects neurons from toxicity induced by $A{\beta}$ by cleaving it into smaller fragments. Thus, dis-regulation of peptidase G could contribute amyloid deposit found in AD brain.

• Journal of Microbiology and Biotechnology
• /
• 제16권2호
• /
• pp.200-204
• /
• 2006

The enzymology of an activated sludge system for a petroleum wastewater purification process was investigated. Leucine-aminopeptidase (L-AMP), ${\beta}$-glucosidase (${\beta}-GLC$), and lipase (LIP) were selected for the study. It was found that more than 81.7% of enzymatic activity was associated with microbial cells in the activated sludge floc. The metabolic response of a mixed microbial population to increased phenol concentration showed that L-AMP activity increased in the activated sludge, whereas activities of ${\beta}-GLC$ and LIP decreased, due to the inhibitory effect of the phenol which varied from 100 mg/l to 500 mg/l.

• 한국생명과학회:학술대회논문집
• /
• 한국생명과학회 2005년도 국제학술심포지움 The 44th Annual Meeting of Korean Society for Life Science
• /
• pp.110-110
• /
• 2005