Browse > Article

Enzymatic Activities in Petroleum Wastewater Purification System by an Activated Sludge Process  

Li Yin (College of Environmental Science & Engineering, Dong Hua University)
Chrost Ryszard J. (Department of Microbial Ecology, Institute of Microbiology, Warsaw University)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.2, 2006 , pp. 200-204 More about this Journal
Abstract
The enzymology of an activated sludge system for a petroleum wastewater purification process was investigated. Leucine-aminopeptidase (L-AMP), ${\beta}$-glucosidase (${\beta}-GLC$), and lipase (LIP) were selected for the study. It was found that more than 81.7% of enzymatic activity was associated with microbial cells in the activated sludge floc. The metabolic response of a mixed microbial population to increased phenol concentration showed that L-AMP activity increased in the activated sludge, whereas activities of ${\beta}-GLC$ and LIP decreased, due to the inhibitory effect of the phenol which varied from 100 mg/l to 500 mg/l.
Keywords
Enzymatic activity; activated sludge; phenol; petroleum wastewater;
Citations & Related Records

Times Cited By Web Of Science : 1  (Related Records In Web of Science)
연도 인용수 순위
  • Reference
1 Boszczyk-Maleszak, H., M. Chorazy, E. Bieszkiewicz, and J. Kacieszczenko. 2002. Phenol utilisation by fungi isolated from activated sludge. Acta Microb. Pol. 51: 183-191
2 Cadoret, A., A. Conrad, and J.-C. Block. 2002. Availability of low and high molecular weight substrates to extracellular enzymes in whole and dispersed activated sludges. Enzyme Microb. Tech. 31: 179-186   DOI   ScienceOn
3 Avci, A., M. Kacmaz, and I. Durak. 2005. Peroxidation in muscle and liver tissues from fish in a contaminated river due to a petroleum refinery industry. Ecotox. Environ. Safe. 60: 101-105   DOI   ScienceOn
4 Barjenbruch, M. and O. Kopplow. 2003. Enzymatic, mechanical and thermal pre-treatment of surplus sludge. Adv. Environ. Res. 7: 715-720   DOI   ScienceOn
5 Kibret, M., W. Somitsch, and K.-H. Robra. 2000. Characterization of a phenol degrading mixed population by enzyme assay. Water Res. 34: 1127-1134   DOI   ScienceOn
6 Czerska, B., K. Miksch, N. Matsche, and A. Franz. 1997. Application of enzymatic activity measurements to biological phosphate removal from wastewater. Water Sci. Tech. 36: 87-95
7 Nguyen, V. T. and W. K. Shieh. 1995. Anoxic and oxic biological fluidized bed treatment of amines and phenol. Water Sci. Tech. 31: 185-193
8 Whiteley, C. G., J. E. Burgess, X. Melamane, B. Pletschke, and P. D. Rose. 2003. The enzymology of sludge solubilisation utilising sulphate-reducing systems: The properties of lipases. Water Res. 37: 289-296   DOI   ScienceOn
9 Guellil, A., F. Thomas, J.-C. Block. J.-L. Bersillon, and P. Ginertet. 2001. Transfer of organic matter between wastewater and activated sludge flocs. Water Res. 35: 143- 150   DOI   ScienceOn
10 Gessesse, A., T. Dueholm, S. B. Petersen, and P. H. Nielsen. 2003. Lipase and protease extraction from activated sludge. Water Res. 37: 3652-3657   DOI   ScienceOn
11 Seetharam, G. B. and B. A. Saville. 2003. Degradation of phenol using tyrosinase immobilized on siliceous supports. Water Res. 37: 436-440   DOI   ScienceOn
12 Lopez-Molina, D., A. N. P. Hiner, J. Tudela, F. Garcia- Canovas, and J. N. Rodriguez-Lopez. 2003. Enzymatic removal of phenols from aqueous solution by artichoke (Cynara scolymus L.) extracts. Enzyme Microb. Tech. 33: 738-742   DOI   ScienceOn
13 Raunkjaer, K., T. Hvitved-Jacobsen, and P. H. Nielsen. 1994. Measurement of pools of protein, carbohydrate and lipid in domestic wastewater. Water Res. 28: 251-262   DOI   ScienceOn
14 APHA, 1998. Standard Methods for Examination of Water and Wastewater, 20th Ed. American Public Health Association, Washington, D.C., U.S.A
15 Gianfreda, L., F. Sannino, M. A. Rao, and J.-M. Bollag. 2003. Oxidative transformation of phenols in aqueous mixtures. Water Res. 37: 3205-3215   DOI   ScienceOn
16 Wingender, J., K.-E. Jaeger, and H.-C. Flemming. 1999. Interaction between extracellular polysaccharides and enzymes, pp. 231-251. In Wingender, J., T. R. Neu, and H.-C. Flemming (eds.). Microbial Extracellular Substrances: Characterization, Substructure and Function. Springer, New York, U.S.A
17 Ou, S., J. Zheng, J. Zheng, B. J. Richardson, and P. K. S. Lam. 2004. Petroleum hydrocarbons and polycyclic aromatic hydrocarbons in the surficial sediments of Xiamen Harbour and Yuan Dan Lake, China. Chemosphere 56: 107-112   DOI   ScienceOn