• The Microorganisms and Industry
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• v.13 no.1
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• pp.10-13
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• 1987

L-Azetidine-2-carboxylic acid(A-2-C) is a four-membered cyclic imino acid which was first discovered from Convalaria majalis and Polygonatum officinalis in 1955(1,2). The imino acid A-2-C has been identified in at least 16 species of plants (3) (mostly the families Liliaceae, Agavaceae and Amaryllidaceae); in two marine sponges (Haliclona sp. and Chalinospilla sp.) (4); in the red algae (Lophocladia lamenandi) (5); in the sugar beet Beta vulgaris (6) ; and the microorganism Actinoplanes ferrugineus (7).

• Natural Product Sciences
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• v.16 no.4
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• pp.228-232
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• 2010

Bioactivity-guided fractionation using on-line HPLC biochemical detection system on $CHCl_3$-soluble fraction of Lycoris radiata led to the isolation of deoxylycorenine (1), O-demethylhomolycorine (2), galanthamine (3), lycoramine (4), mixture of $6{\alpha}$-and $6{\beta}$-haemanthidine (5), and lycorine (6), identified by spectroscopic data and physicochemical property. Among the isolated compounds, 1, 3 and 6 showed acetylcholinesterase inhibitiory activities with $IC_{50}$ values of 18.0, 12.0 and $16.6\;{\mu}M$, respectively, in in vitro colorimetric microplate assay.

• Natural Product Sciences
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• v.17 no.2
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• pp.100-103
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• 2011

Phytochemical study on the ethanol extract of a Crinum species by bioassay-guided fractionation resulted in the isolation of an active principle, which was determined as parthenin (1) on the basis of physicochemical and spectroscopic analyses. This is the first report on the existence of a sesquiterpenoid from Crinum ensifolium. Compound 1 was found to show strong cytotoxic activity against some cancer cell lines and strongly inhibit NF-${\kappa}$B activity with the $IC_{50}$ value of 1.82 ${\mu}M$.

• Korean Journal of Plant Resources
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• v.21 no.3
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• pp.226-229
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• 2008

We described and illustrated a rare species in Korea, Hypoxis aurea Lour. (Hypoxidaceae) which was rediscovered about 70 years after its first collection from Jeju island in Korea. The members of the family Hypoxidaceae R. Br. are distinguished from the plants of Amaryllidaceae J. St-Hill. by having grass-like leaves, an invisible stem which is modified into a corm or a rhizome, trimerous, and radially symmetric flowers with an inferior ovary developing into a capsule on scapes. Hypoxis aurea Lour. is readily distinguishable from Curculigo orchinoides Gsertn. in Japan by beakless ovary and capsular fruit. The number of somatic chromosome is 2n=54.

• Natural Product Sciences
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• v.20 no.2
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• pp.107-112
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• 2014

This research aimed to investigate the antibacterial activity of Lycoris radiata. The methanol extract and solvent fractions from L. radiata exhibited antibacterial activities against Escherichia coli, Staphylococcus aureus, and Helicobactor pylori. Open-column chromatography was used to isolate phytochemical constituents from L. radiata; spectroscopic analysis elucidated their structures as ${\beta}$-sitosterol (1), daucosterol (2), O-methyllycorenine (3), lycorenine (4), lycoricidinol (5), lycorine (6), and lycoricidine (7). Further testing of compounds 1 - 7 revealed antibacterial effects against E. coli, S. aureus, and H. pylori, which suggested the potential of these substances as antibacterial agents. We determined that compounds 1 and 2, isolated from the n-hexane fraction, were more effective against S. aureus and H. pylori. Compound 4, isolated from the methylene chloride fraction, exhibited noticeable antibacterial effects against E. coli. This study is the first report on the antibacterial activities of phytochemical constituents from L. radiata against E. coli, S. aureus, and H. pylori.

• The Korean Journal of Oral and Maxillofacial Pathology
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• v.41 no.1
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• pp.1-7
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• 2017

Lycorine, a natural alkaloid extracted from the Amaryllidaceae plant family, was reported to various physiological and pharmacological effects including anti-cancer activity. Nevertheless, there is no report of the anticancer effect of lycorine in oral cancer cells. The effects of lycorine on cell proliferation and apoptosis were examined through trypan blue exclusion assay, 4'-6-diamidino-2-phenylindole (DAPI) stain, Live/Dead assay, Western blot analysis and RT-PCR. Lycorine suppressed cell viability and induced apoptosis in MC3 and HSC-3 cell lines. Lycorine decreased survivin protein but did not affect its mRNA. It regulated survivin through accelerating protein degradation in a time-dependent manner although neither proteasome nor lysosome was not associated with lycorine-mediated protein degradation. Collectively, our results suggest that lycorine may be a potential therapeutic anti-cancer drug candidate for the treatment of human oral cancer.

• Advances in Traditional Medicine
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• v.6 no.4
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• pp.274-285
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• 2006

Allium sativum (Family Amaryllidaceae or Liliaceae) is used worldwide for various clinical uses like hypertension, cholesterol lowering effect, antiplatelets and fibrinolytic activity etc. Due to these common house hold uses of Allium sativum, as a herbal supplements, and failure of patients to inform their physician of the over-the-counter supplements they consume leads to drugnutrient interactions with components in herbal supplements. Today these types of interactions between a herbal supplement and clinically prescribed drugs are an increasing concern. In vitro studies indicated that garlic constituents modulated various CYP (cytochrome P450) enzymes. CYP 3A4 is abundantly present in human liver and small intestine and contributes to the metabolism of more than 50% of commonly used drugs including nifedipine, cyclosporine, erythromycin, midazolam, alprazolam, and triazolam. Extracts from fresh and aged garlic inhibited CYP 3A4 in human liver microsomes. The in vivo effects of garlic constituents are found to be species depended and the dosing regimen of garlic constituents appeared to influence the modulation of various CYP isoforms. Studies have indicated that the inhibition of various CYPs by organosulfur compounds from garlic was related to their structure also. Studies using in vitro, in vivo, animal and human models have indicated that various garlic constituents can be the substrates, inhibitors and or inducers of various CYP enzymes. The modulation of CYP enzyme activity and expression are dependent on the type and chemical structure of garlic constituents, dose regime, animal species and tissue, and source of garlic thus this review throws light on the possible herb drug interaction with the use of garlic.

• Korean Journal of Plant Taxonomy
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• v.38 no.1
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• pp.17-29
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• 2008

Phylogenetic relationships were examined for 17 taxa of Lycoris by RAPD analysis. The length of the amplified DNA fragments ranged from 300 bp to 1,700 bp. 57 scorable RAPD markers were observed from PCR reactions with five random oligoprimers. The analysis by UPGMA sepatated the examined taxa of Lycoris into were clusters. First group was comprised of ten taxa of L. chinensis var. sinuolata, L. sanguinea var. koreana, L. uydoensis, L. flavescens, L. radiata var. pumila, L. radiata, L. squamigera, L. chejuensis, L. aurea and L. guangxiensis, second group of L. haywardii, L. sprengeri, L. rosea, L. straminea and L. houdyshii, third group of outgroup of Narcissus tazetta var. chinensis and Crinum asiaticum var. japonicum. From the viewpoint of cytological characters such as polyploidy and karyotype, the RAPD analysis was very useful to show the relationship among the intraspecific taxa of Lycoris.

• Journal of Plant Biotechnology
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• v.47 no.1
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• pp.15-25
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• 2020

Allium L. is one of the largest genera of the Amaryllidaceae family, with more than 920 species including many economically important species used as vegetables, spices, medicines, or ornamental plants. Currently, DNA barcoding tools are being successfully used for the molecular taxonomy of Allium. A total of 46 Allium species were collected from their native areas, and DNA was extracted using the IBRC DNA extraction kit. We used specific primers to PCR amplify matK. DNA sequences were edited and aligned for homology, and a phylogenetic tree was constructed using the neighbor-joining method. The results show thymine (38.5%) was the most frequent and guanine (13.9%) the least frequent nucleotide. The matK regions of the populations were quite highly conserved, and the amount of C and CT was calculated at 0.162 and 0.26, respectively. Analysis of the nucleotide substitution showed C-T (26.22%) and A-G (8.08%) to have the highest and lowest percent, respectively. The natural selection process dN/dS was 1.16, and the naturality test results were -1.5 for Tajima's D and -1.19 for Fu's Fs. The NJ dendrogram generated three distinct clades: the first contained Allium austroiranicum and A. ampeloprasum; the second contained A. iranshahrii, A. bisotunense, and A. cf assadi; and the third contained A. rubellum and other species. In this study, we tested the utility of the matK region as a DNA barcode for discriminating Allium. species.

• Korean Journal of Plant Taxonomy
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• v.48 no.4
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• pp.363-369
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• 2018

Since the establishment of the Korean Society of Plant Taxonomists (KSPT) on Dec. 13, 1968, the professional manpower training and research activities have been analyzed. The survey method was based on the homepage of the KSPT and the Korean J. Pl. Taxon, and on data provided by each university about professional manpower. Over the past 50 years, a total of 680 specialists in plant taxonomy have been trained, consisting of 537 master's degree holders (274 males, 263 females) from 30 universities and 143 PhDs (97 males, 46 females) from 26 universities, and the number has increased significantly since 1998. With regard to changes in the field of research over the last ten years, revision papers were the most common in the period of 1988-1997 (72%), but this rate has decreased to 51% over the last ten years, while the number of unrecorded papers has increased to 28%. In the 629 revision papers on taxa, 49% of the taxa belong to Asteraceae, Ranunculaceae, Cyperaceae, Liliaceae, Rosaceae, Fabaceae, Apiaceae, Lamiaceae, Orchidaceae, Oleaceae, Euphorbiaceae, Polygonaceae, and Amaryllidaceae. With regard to changes in research methods, the number of morphological papers increased from 6% to 51%, while pollen papers have decreased from a rate of 20% to only 2%. Chromosome studies account for 3-4%, chemotaxonomic studies 2%, and DNA studies remain low at 3-16%. The percentage of papers in English now stands at 43%, mainly due to the increased number of papers on unrecorded species.