Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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The Isolation of Acetylcholinesterase Inhibitory Constituents from Lycoris radiata using On-line HPLC-biochemical Detection System

  • Yang, Hee-Jung (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University) ;
  • Yoon, Kee-Dong (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University) ;
  • Chin, Young-Won (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University) ;
  • Kim, Young-Choong (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University) ;
  • Kim, Jin-Woong (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
  • Received : 2010.09.06
  • Accepted : 2010.11.26
  • Published : 2010.12.31
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Abstract

Bioactivity-guided fractionation using on-line HPLC biochemical detection system on $CHCl_3$-soluble fraction of Lycoris radiata led to the isolation of deoxylycorenine (1), O-demethylhomolycorine (2), galanthamine (3), lycoramine (4), mixture of $6{\alpha}$-and $6{\beta}$-haemanthidine (5), and lycorine (6), identified by spectroscopic data and physicochemical property. Among the isolated compounds, 1, 3 and 6 showed acetylcholinesterase inhibitiory activities with $IC_{50}$ values of 18.0, 12.0 and $16.6\;{\mu}M$, respectively, in in vitro colorimetric microplate assay.

Keywords

References

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