• Journal of Microbiology
• /
• v.43 no.5
• /
• pp.383-390
• /
• 2005

The diversity of the denitrifying bacterial populations in Daejeon Sewage Treatment Plant was examined using a culture-dependent approach. Of the three hundred and seventy six bacterial colonies selected randomly from agar plates, thirty-nine strains that showed denitrifying activity were selected and subjected to further analysis. According to the morphological and biochemical properties, the thirty nine isolates were divided into seven groups. This grouping was supported by an unweighted pair group method, using an arithmetic mean (UPGMA) analysis with fatty acid profiles. Restriction pattern analysis of 16S rDNA with four endonucleases (AluI, BstUI, MspI and RsaI) again revealed seven distinct groups, consistent with those defined from the morphological and biochemical properties and fatty acid profiles. Through the phylogenetic analysis using the 16S rDNA partial sequences, the main denitrifying microbial populations were found to be members of the phylum, Proteobacteria; in particular, classes Gammaproteobacteria (Aeromonas, Klebsiella and Enterobacter) and Betaproteobacteria (Acidovorax, Burkholderia and Comamonas), with Firmicutes, represented by Bacillus, also comprised a major group.

• The Plant Pathology Journal
• /
• v.17 no.1
• /
• pp.57-61
• /
• 2001

Phytoplasmas were identified from two chrysanthemum (Dendranthema grandiflorum) plants showing different symptoms ; one with stusting, rosette, and excessive branching (Ph-ch1), and the other with stunting and chlorosis (Ph-ch2). Electron microscopy of midrib of the plants with the symptoms revealed that numerous phytoplasmas were localized in the phloem cells. The disease was transmitted from infected plants to healthy ones by grafting. Phytoplasma-specific DNA was detected in polymerase chain reaction (PCR) analysis with template DNA extracted from the leaves of Ph-ch1 and Ph-ch2, both of which yielded a same DNA band corresponding to 1.5 kb. Using a specific primer pair (R16F1/R1) synthesized based on aster yellows (AY) phytoplasma, a DNA fragment of 1.1 kb was amplified by PCR. Endonuclease restriction patterns of the 1.1 kb PCR products from Ph-ch1 and Ph-ch2, which were dgeste with each of the restriction endonucleases Sau3A, Hha, Alu and Rsa, were same as those of AY phytoplasma from periwinkle. This suggests that the chrysanthemum plants (Ph-ch1 and Ph-ch2) be infected with a phytoplasma belonging to AY phytoplasma.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2021.06a
• /
• pp.287-287
• /
• 2021

본 연구에서는 CUDA(Compute Unified Device Architecture) 포트란을 이용하여 확산파 강우 유출모형을 개발하였다. CUDA 포트란은 그래픽 처리 장치(Graphic Processing Unit: GPU)에서 수행하는 병렬 연산 알고리즘을 포트란 언어를 사용하여 작성할 수 있도록 하는 GPU상의 범용계산(General-Purpose Computing on Graphics Processing Units: GPGPU) 기술이다. GPU는 그래픽 처리 작업에 특화된 다수의 산술 논리 장치(Arithmetic Logic Unit: ALU)로 구성되어 있어서 중앙 처리 장치(Central Processing Unit: CPU)보다 한 번에 더 많은 연산 수행이 가능하다. 이에 따라, CUDA 포트란기반 확산파모형은 분포형 강우유출모형의 수치모의 연산시간을 단축시킬 수 있다. 분포형모형의 지배방정식은 확산파모형과 Green-Ampt모형으로 구성되었고, 확산파모형은 유한체적법을 이용하여 이산화 하였다. CUDA 포트란기반 확산파모형의 정확성은 기존 연구된 수리실험 결과 및 CPU기반 강우유출모형과 비교하였으며, 연산소요시간에 대한 효율성은 CPU기반 확산파모형과 비교하였다. 그 결과 CUDA 포트란기반 확산파모형의 결과는 수리실험 결과 및 CPU기반 강우유출모형의 결과와 유사한 결과를 나타냈다. 또한, 연산소요시간은 CPU 기반 확산파모형의 연산소요시간보다 단축되었으며, 본 연구에 사용된 장비를 기준으로 최대 100배 정도 단축되었다.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2020.06a
• /
• pp.323-323
• /
• 2020

그래픽 처리 장치(Graphic Processing Unit: GPU)는 그래픽 처리 작업에 특화된 다수의 산술논리 장치(Arithmetic Logic Unit: ALU)로 구성되어 있어서 중앙 처리 장치(Central Processing Unit: CPU)보다 한 번에 더 많은 연산 수행이 가능하다. 본 연구는 GPU 가속 운동파모형을 실제 유역에 적용하여, GPU 가속 운동파 강우유출모형 결과에 대한 정확성과 연산 소요 시간에 대한 효율성을 확인하였다. GPU 가속 운동파모형은 분포형 강우유출모형의 수치모의 연산시간을 단축시키기 위해 CUDA 포트란을 이용하여 개발되었다. 분포형모형의 지배방정식은 운동파모형과 Green-Ampt모형으로 구성되었고, 운동파모형은 유한체적법을 이용하여 이산화 하였다. GPU 가속 운동파모형을 이용하여 금강의 미호천 유역에서 발생하는 강우유출현상을 모의 하였고, 동일한 유한체적법을 이용한 CPU(Central Processing Unit) 기반의 강우유출모형과 비교하였다. 그 결과 GPU 가속모형의 결과는 미호천 유역 하류단에서 관측한 결과와 유사한 결과를 나타냈다. 또한, 연산소요시간은 CPU 기반의 강우유출모형의 연산소요시간보다 단축되었으며, 본 연구에 사용된 장비를 기준으로 최대 100배 정도 단축되었다.

• Asian-Australasian Journal of Animal Sciences
• /
• v.21 no.4
• /
• pp.484-488
• /
• 2008

We investigated the effect of the prolactin receptor gene (PRLR) on total number of born (TNB), number of born alive (NBA) and number of weaned (NW) piglets in Large White (LW), White Meaty (WM) and Landrace (L) sows from six Slovak breeding farms. The frequency of A allele was 0.48, 0.49 and 0.47 in LW, WM and L, respectively. We found numerous highly significant effects of PRLR locus on TNB ($p{\leq}0.01$; $p{\leq}0.05$) in all tested breeds. The most marked difference of +$1.31{\pm}0.45pigs/L$ was found between AA and BB genotypes in WM. Within the other breeds the difference between the homozygous genotypes reached up to +$0.94{\pm}0.3$ and +$1.21{\pm}0.19$ pigs per litter in LW and L, respectively. We also identified significant differences between AA and AB genotypes related to TNB in L. Similarly NBA, as well as NW traits were significantly affected ($p{\leq}0.01$; $p{\leq}0.05$) by the genotype just in LW and L. The homozygous genotype AA was favourable in all breeds and traits. Our results showed the possibility of PRLR utilization in marker-assisted selection within breeding programs to increase reproductive traits of pigs in Slovakia.

• Journal of the Institute of Electronics Engineers of Korea SD
• /
• v.43 no.9 s.351
• /
• pp.53-58
• /
• 2006

A Vertex Shader is designed to show more 3D graphics expressions, and to increase flexibility of the fixed function T&L (Transform and Lighting) engine. Design of this Shader is based on Vertex Shader 1.1 of DirectX 8.1 and OpenGL ARB. The Vertex Shader consists of four floating point ALUs for vectors operation. The previous 32bits floating point data type is replaced to 24bits floating point data type in order to design the Vertex Shader that consume low-power and occupy small area. A Xilinx Virtex2 300M gate module is used to verify behaviour of the core. The result of Synopsys synthesis shows that the proposed Vertex Shader performs 115MHz speed at the TSMC 0.13um process and it can operate as the rate of 12.5M Polygons/sec. It shows the complexity of 110,000 gates in the same process.

• Journal of the Institute of Electronics Engineers of Korea SD
• /
• v.39 no.2
• /
• pp.72-81
• /
• 2002

A new high-performance DSP architecture is proposed, which behaves as a coprocessor of a 32bit microcontroller. Because the proposed DSP architecture is a dual MAC(Multiply and Accumulate) DSP architecture, it can process efficiently a number of SOP(sum of product) operations used in many DSP applications. In order to efficiently perform other operations such as pure additions without any restriction, a MAC is composed of a multiplier and a ALU placed in parallel. In addition, it is a 3-way superscalar architecture, which can issue 3 instructions at a time. The benchmark results with 3 thor dual MAC DSPs show that the proposed DSP has the best performance. Futhermore, it is proven that the proposed DSP is more efficient in memory usage, although the performance is comparable in some algorithms such as Viterbi decoding and FFT butterfly.

• The Plant Pathology Journal
• /
• v.33 no.2
• /
• pp.140-147
• /
• 2017

Fusarium wilts of strawberry, caused by Fusarium oxysporum f. sp. fragariae, is a serious soil-borne disease. Fusarium wilt causes dramatic yield losses in commercial strawberry production and it is a very stubborn disease to control. Reliable chemical control of strawberry Fusarium wilt disease is not yet available. Moreover, other well-known F. oxysporum have different genetic information from F. oxysporum f. sp. fragariae. This analysis investigates the genetic diversity of strawberry Fusairum wilt pathogen. In total, 110 pathogens were isolated from three major strawberry production regions, namely Sukok, Hadong, Sancheong in Gyeongnam province in South Korea. The isolates were confirmed using F. oxysporum f. sp. fragariae species-specific primer sets. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analyses were executed using the internal transcribed spacer, intergenic spacer, translation elongation factor1-${\alpha}$, and ${\beta}$-tubulin genes of the pathogens and four restriction enzymes: AluI, HhaI, HinP1I and HpyCH4V. Regarding results, there were diverse patterns in the three gene regions except for the ${\beta}$-tubulin gene region. Correlation analysis of strawberry cultivation region, cultivation method, variety, and phenotype of isolated pathogen, confirmed that genetic diversity depended on the classification of the cultivated region.

• Journal of Microbiology and Biotechnology
• /
• v.20 no.1
• /
• pp.193-201
• /
• 2010

A specific and rapid real-time PCR assay for detecting Ralstonia solanacearum in horticultural soil and plant tissues was developed in this study. The specific primers RSF/RSR were designed based on the upstream region of the UDP-3-O-acyl-GlcNAc deacetylase gene from R. solanacearum, and a PCR product of 159 bp was amplified specifically from 28 strains of R. solanacearum, which represent all genetically diverse AluI types and all 6 biovars, but not from any other nontarget species. The detection limit of $10^2\;CFU/g$ tomato stem and horticultural soil was achieved in this real-time PCR assay. The high sensitivity and specificity observed with field samples as well as with artificially infected samples suggested that this method might be a useful tool for detection and quantification of R. solanacearum in precise forecast and diagnosis.

• Journal of Microbiology and Biotechnology
• /
• v.21 no.10
• /
• pp.1001-1011
• /
• 2011

To explore bacterial diversity for elucidating genetic variability in acylhomoserine lactone (AHL) lactonase structure, we screened 800 bacterial strains. It revealed the presence of a quorum quenching (QQ) AHL-lactonase gene (aiiA) in 42 strains. These 42 strains were identified using rrs (16S rDNA) sequencing as Bacillus strains, predominantly B. cereus. An in silico restriction endonuclease (RE) digestion of 22 AHL lactonase gene (aiiA) sequences (from NCBI database) belonging to 9 different genera, along with 42 aiiA gene sequences from different Bacillus spp. (isolated here) with 14 type II REs, revealed distinct patterns of fragments (nucleotide length and order) with four REs; AluI, DpnII, RsaI, and Tru9I. Our study reflects on the biodiversity of aiiA among Bacillus species. Bacillus sp. strain MBG11 with polymorphism (115Alanine > Valine) may confer increased stability to AHL lactonase, and can be a potential candidate for heterologous expression and mass production. Microbes with ability to produce AHL-lactonases degrade quorum sensing signals such as AHL by opening of the lactone ring. The naturally occurring diversity of QQ molecules provides opportunities to use them for preventing bacterial infections, spoilage of food, and bioremediation.