• 한국축산식품학회지
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• 제40권4호
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• pp.659-667
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• 2020

Muscle stem cells isolated from domestic animals, including cows and pigs, were recently spotlighted as candidates for the production of alternative protein resources, so-called cultured meat or lab-grown meat. In the present study, we aimed to optimize the in vitro culture conditions for the long-term expansion of pig muscle stem cells via the screening of various signaling molecules. Pig muscle stem cells were collected from the biceps femoris muscles of 3-d-old crossbred pigs (Landrace×Yorkshire×Duroc, LYD) and cultured in minimum essential medium-based growth media. However, the pig muscle stem cells gradually lost their proliferation ability and featured morphologies during the long-term culture over two weeks. To find suitable in vitro culture conditions for an extended period, skeletal muscle growth medium-2, including epidermal growth factor (EGF), dexamethasone, and a p38 inhibitor (SB203580), was used to support the stemness of the pig muscle stem cells. Interestingly, pig muscle stem cells were stably maintained in a long-term culture without loss of the expression of myogenic marker genes as determined by PCR analysis. Immunostaining analysis showed that the stem cells were capable of myogenic differentiation after multiple passaging. Therefore, we found that basal culture conditions containing EGF, dexamethasone, and a p38 inhibitor were suitable for maintaining pig muscle stem cells during expanded culture in vitro. This culture method may be applied for the production of cultured meat and further basic research on muscle development in the pig.

• 한국가금학회지
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• 제38권4호
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• pp.275-284
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• 2011

As the world population grows and developing countries become more affluent, the global consumption of meat will increase by more than 50% within the next 10 years. Confronting the increased demand for poultry food products are emerging field diseases, increasing regulatory bans of antimicrobial growth promoters, high-density growth conditions, and waste management. Although biotechnology offers solutions to some of these challenges, basic studies are needed to better understand the complex interaction between the intestinal microbiome, host immunity and the environment. This presentation will focus on emerging strategies to enhance gut immunity and to decrease economic losses due to poultry diseases. This presentation will highlight recent developments in coccidiosis research and provide information on host immunity, immunomodulation, and the latest advances in dietary and nutritional approaches against coccidiosis. Such information will magnify our understanding of host-parasite biology, mucosal immunology, and design of future nutritional interventions and vaccination strategies for coccidiosis.

• 한국식품영양학회지
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• 제22권4호
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• pp.612-617
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• 2009

This study was conducted to assess the information of food safety by college students according to mass-media reports. To accomplish this, a survey was conducted to investigate the preference and reliability, of primary mass-media sources of BSE information, and BSE-related knowledge of college students. The most common sources of media by male and female students were terrestrial television(60%), the internet(21%) and newspapers(11%) and terrestrial television(60%), newspapers (18%) and the internet(17%), respectively. The reliability of media of male students were terrestrial television(53%) and the internet(40%), while for female students these values were terrestrial television(55%) and internet(37%), respectively. In the case of male students, the primary sources of information regarding BSE were terrestrial television(54%) and the internet(38%), but for female students the primary sources were terrestrial television(57%) and the internet(37%). Both male and female students were found to have less knowledge regarding prions, SRM, and MM type genes associated with BSE when compared to the other factors associated with this disease. In addition, the important determining factors for the food purchase of male and female students both were the orders of taste, hygiene, price. The preferences for meats and the beef-alternative meats of male and female students were the orders of pork, chicken. Based on these results, greater efforts should be made to provide meaningful information regarding the safe production and distribution of meats.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.2122-2122
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• 2001

The ultra-high pressure technology fur the preservation of foods is under intense research to evaluate its potential as an alternative or complementary process to traditional methods of food preservation. Traditional processing methods usually need a large amount of energy, may cause unwanted reactions in the food, leading to cooked flavor and loss of vitamins, etc. The application of ultra-high hydrostatic pressure for food processing consists of subjecting the food to pressures in the range of 100-1000 ㎫. The ultra-high pressure inactivates the microorganisms and some enzymes, promotes the germination of spores and extends the shelf-life of the foods. This new technology follows the “minimal processing” concept minimizing the quality degradation, saving the vitamins, essential nutrients and flavors as well as utilizing less energy. We joined the research team at our University involved in the mentioned technology using an ultra-high pressure equipment, recording of the near infrared spectra and signal response of a chemosensor array (electronic nose) of their meat (beef and pork), vegetable and fruit samples exposed to different pressure. The results of our investigations achieved by evaluating the measured data using PCA and PQS methods will be presented.

• 한국축산식품학회지
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• 제36권5호
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• pp.567-576
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• 2016

Poultry industry has always been a dynamic and integral part of national economies in many countries. Economic losses incur especially in large-scale rearing facilities, often attributed to the deterioration of environmental conditions, poultry exposure to stressors and development of diseases. While antibiotics have been commonly used for prophylactic purposes and as growth stimulants, extensive documentation of antimicrobial resistance among pathogenic bacteria due to indiscriminate utilization of antibiotic in the industry has led to public and governmental outcries. Elimination of antibiotics from poultry production has thus encouraged intensive search for alternatives. In this review, we discuss the immense potential of probiotics to fill the gap as alternative growth promoters and evidences of beneficial effects of probiotic application in poultry production.

• 한국축산식품학회지
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• 제38권1호
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• pp.14-25
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• 2018

Chemical compositions, trypsin inhibitory activity, and gelling properties of albumen from duck egg during salting of 30 days were studied. As the salting time increased, moisture content decreased, the salt content and surface hydrophobicity increased (p<0.05). Trypsin inhibitory activity and specific activity were continuously decreased throughout the salting time of 30 days (p<0.05). This coincided with the decrease in band intensity of inhibitor with molecular weight of 44 kDa as examined by inhibitory activity staining. Nevertheless, no differences in protein patterns were observed in albumen during the salting of 30 days. Based on texture profile analysis, hardness, springiness, gumminess, chewiness, and resilience of albumen gel decreased with increasing salting time. Conversely, salted albumen gels exhibited higher cohesiveness and adhesiveness, compared to those of fresh albumen. Scanning electron microscopic study revealed that gel of salted albumen showed the larger voids and less compactness. In general, salting lowered trypsin inhibitory activity and gelling property of albumen from duck egg to some extent. Nevertheless, the salted albumen with the remaining inhibitor could be an alternative additive for surimi or other meat products to prevent proteolysis.

• 한국식품과학회지
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• 제37권2호
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• pp.294-300
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• 2005

식품의 안전성 평가에 사용되는 주요 지표 미생물들의 검출 시험법 중에서 전통적인 미생물 시험법과 신속 검출법인 건조 필름법에 의한 미생물 시험법 간의 유효성 관계를 국내 특정 축 수산식품에 존재하는 미생물을 대상으로 분석하였다. 축 수산식품 중 어묵, 게맛살, 아이스크림, 우유, 햄 시료에 대하여 두 가지 미생물 시험법을 각각 실시하였다. 일반 세균수 분석에는 plate count agar법과 $Petrifilm^{TM}$ aerobic count plate법을 비교하였고, 대장균군과 대장균의 분석에는 most probable number(MPN)법과 $Petrifilm^{TM}$ coliform count plate 및 $Petrifilm^{TM}$ E. coli/coliform count plate법을 각각 비교하였으며, 효모와 곰팡이의 분석에는 potato dextrose agar법과 $Petrifilm^{TM}$ yeast and mold count plate법을 비교하였다. 황색포도상구균의 분석에는 coagulase 시험법과 $Petrifilm^{TM}$ staph express count plate법을 비교하였다. 5가지 축 수산식품에 대한 두 방법들간 상관계수는 일반세균이 $0.990{\sim}0.999$, 대장균군이 0.979-0.987, 대장균이 0.978-0.984, 효모와 곰팡이가 0.975-0.999, 황색포도상구균이 0.999로 두 방법간 상관성이 매우 높았으며, 평균 미생물 수에 있어서도 유의적인 차이가 없는 것으로 확인되었다. 이상의 결과로부터 건조필름법이 기존의 전통적인 시험 방법을 대체할 수 있는 미생물 분석법임이 확인되었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1998년도 21세기 한반도 농업전망과 대책(한국작물학회.한국육종학회 공동주관 심포지움 회보)
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• pp.266-287
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• 1998

Environmental conditions and expected profit are the major criteria to select crops to be cultivated in any region. Traditionally, miscellaneous crops have been cultivated as alternative crop in case of unfavorable climate or supplementary crop in marginal lands. Since the successful breeding of high yielding varieties of staple crops and development of cultural techniques in 1970s in Korea, production of miscellaneous crops decreased markedly due to the rapid commercialization of agriculture and increased productions of horticultural crops, although yields have been doubled during the last $2\~3$ decades. On the other hand, animal husbandry has been developing remarkably parallel with national economic development and the increased consumption of animal products. As a result, imports of feed grains such as corn have increased rapidly, while roughage production became lower than demand. Among miscellaneous crops, corn production increased markedly through the development of hybrids and due to its importance as silage crops. Acreage of corn production including grains, fresh corns and feeding is about 120,000 ha, while acreage of buckwheat is about 5,000 ha and those of other miscellaneous crops decreased to a level of about 1,000 ha. Although miscellaneous crops have low yield potential and are unprofitable due to low price and imports of cheap foreign products, they should be kept to cultivate. Miscellaneous crops are important components that maintain diversity among upland crops as well as alternative crops in case of unfavorable climate. The low yield potential of miscellaneous crops might be due to lack of efforts to breed high yielding varieties and to develope cultural techniques. Continuous investment in research, exploitation of new utilization for miscellaneous crops as sustainable crop, honey crop and sightseeing crop, and development of healthy and special foods will promote their cultivation. Animal feeding in Korea depends mainly on formula feeds. As the number of animals increases to meet demand of animal products, there is no alternative way but to import grains to feed them. But roughage production, which is necessary for normal growth of ruminant animals, should be increased. However, lack of arable land and pasture land limits the production of good roughages. It is estimated that number of course for meat and milk production will be $2.5{\~}2.6$ million and total of $6.2{\~}7.5$ million tons of roughages should be produced. This implies that more than 1 million tons of roughage are needed, although pasture land, upland for forage crops, forage crops after rice cultivation and rice straw are utilized. Therefore, new reclamation of pasture land, increased roughage production in cultivating land, increased cultivation of forage crops after rice, more utilization of rice straw and active exploration of indigenous plant species as roughage resources should be promoted

• 한국포장학회지
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• 제24권3호
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• pp.141-148
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• 2018

Photo-oxidation is one of the main causes of food deterioration of great variety of foods, such as dairy products, nuts, meat products, and wine. It causes a loss of both nutritional value and sensorial quality of products and may even leads to the formation of toxic compounds. Active packaging for food and beverages has been investigated and developed with embedding light absorbers or blocking materials into the plastics. In recent years, several novel light barrier materials have been proposed as an alternative option for different applications. This article reviews the up-to-date technology in light absorber and blocking material with special emphasis on chemical compound and mechanism. Inorganic, organic, hybrid organic-inorganic, and natural light absorbers were scoped. The challenges and future perspectives of light barrier materials are also discussed.

• Journal of Animal Science and Technology
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• 제49권6호
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• pp.753-760
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• 2007

본 시험은 돼지에서 면역생물학 제제 투여에 따른 거세 효과를 구명하기 위해서 수행되었다. 공시동물은 2원교잡종(Landrace×Yorkshire) 21일령 자돈 45두를 공시하였고, 시험사육은 출하체중 110kg 도달 때까지 실시하였다. 시험구 배치는 비거세구(NC), 거세구(SC), 면역생물학 제제 투여구(GA)로 나누어 돈방당 5두씩 완전임의 배치하여 3회 반복하였다. 거세는 생후 3-4일령의 자돈에서 외과적 방법으로 실시하였다. 면역생물학 제제 투여는 GnRH antagonist (Improvac, Pfizer Australia)를 시험개시 후 16주와 20주에 각각 두당 2ml씩 2회에 걸쳐 피하주사하였다. 면역생물학 제제 투여에 따른 생산성은 다른 처리구에 비해 유의적인 차이는 나타나지 않았다. 도체특성에서, 정육율과 체지방 함량은 면역생물학 제제 투여에 의해 외과적 거세와 유사한 효과를 나타내었다. 육질 특성에서, 육색, pH, 전단력 및 가열 감량은 유의적인 차이를 나타내지 않았다. 혈중 testosterone 농도는 면역생물학 제제 2차 투여 2주 후에 일반 거세와 유사한 경향을 보였다. 생식기에서 정소 직경, 무게 및 요도구선 무게는 면역생물학 제제 투여에 의해서 비거세 돼지보다 유의적으로 작았다(P=0.002).따라서 본 시험에 이용한 면역생물학 제제를 투여하였을 때 도체 및 육질에 있어서 일반 거세와 비슷한 효과를 나타내었으므로 외과적 수술에 의한 거세를 대처할 수 있을 것으로 사료된다.