• Journal of Life Science
• /
• v.29 no.12
• /
• pp.1386-1392
• /
• 2019

This study investigated the potential of dye plants as natural oral health products. The antibacterial activity of ethanol stem extracts of A. japonica, R. verniciflua Stokes, G. jasminoides, D. morbifera, P. amurense Rupr., and S. japonica against P. gingivalis KCTC 5352, S. mutans KCTC3065, S. downei KCTC3634, S. sanguinis KCTC3284, and S. gordonii KCTC 3286 was confirmed. Among the stem extracts from 6 dye plants grown in Korea, ethanol extract from A. japonica stem (1 mg/disc) showed the highest antibacterial activity against P. gingivalis KCTC5352. The A. japonica stem extracts showed antibacterial activity similar to chlorhexidine, which was used as a positive control. The MIC and MBC of P. gingivalis KCTC5352 were 0.4 mg/ml and 0.6 mg/ml, respectively. The biofilm production rate and cell growth of P. gingivalis KCTC5352 in the cultures treated with 0.2-2.0 mg/ml of A. japonica extract were significantly decreased in a concentration-dependent manner. In addition, the mRNA expression of the superoxide dismutase and fimA associated with fimbriae formation in these cultures was suppressed, also in a concentration-dependent manner. Based on these results, it is concluded that A. japonica stem extracts can be used as an oral health product derived from natural materials, as demonstrated by its antibacterial action against and inhibition of biofilm formation of P. gingivalis KCTC5352.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.17 no.3
• /
• pp.35-47
• /
• 1990

This study was executed to analyze the vegetational landscape structure and administration planning of Heonin Royal Tomb forest by three kinds of ordination techniques (PCA, RA and DCA) and comparison of the couple photographs between 1920s and 1980s. Seventeen sites in the recreation area and fifteen sites in the protective area were sampled with clumped sampling method in June 1988 and five quadrats were examined in each site. Environmental impact grade 3, 4 and 5 area covered 56.4% of the surveyed area and these area should be restored by the input of human energy. Pinus densifora community of actual vegetation covered 8.4%, Alnus japonica 24.2% and Quercus community 40.9% of the total area. And the afforested vegetation of Pinus koraiensis and Pinus rigida covered 23.1 % The recreation area was divided by P. densiflora, P. densiflora-Quercus aliena, A. japonica-Q.aliena, A. japonica and the protective area by Q. acutissima, Q. aliena, A. japonica-Q. aliena.. DCA ordination showed that successional trends of tree species seem to be from P. densiflora, Sorbus alnifolia, Styrax obassia to Q. variabilis Q. serrata in P. densiflora community and from A. japonica through A. ginnala to Q. aliena in A. japonica community of the upper layer. By the comparison of the couple photographs between 1920s and 1980s, we can recognize the change of historical landscape composed of P. densiflora community and those community is succeeded to Q. aliena.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.25 no.4
• /
• pp.123-140
• /
• 1998

The flora of Kunsan Wolmyong park was 77 families, 168 genera, 240 species, 2 subspecies, 35 varieties, 1 formae or 278taxa. Using the Phytosociology method, 12 plant communities were recognized ; Alnus japonica, Pinus densiflora, Pinus thunbergii, Pinus rigida, Castanea crenata, Quercus acutissima, Robinia pseudo-acacia, Alnus hirsuta, Quercus serrata, Aluns firma, Liriope platyphylla and Reynoutria communities. Similarity between different communities was low for most of the cases except for Pinus densiflora, Pinus Thunbergii and Pinus rigida communities kept comparely high with ond another. Species diversity was analyzed by species richness(SR), species diversity index(H') and evenness(J'). Castanea crenata, Robinia pseudo-acacia and Alnus firma communities were higher in SR, H' than the other communities. But Pinus rigida, Quercus acutissima and castanea crenata communities were higher in evenness than the other communities. To investigate the plant community structure and to establish restoration counter plan of a Wolmyeng Park in Kunsan City. As a remedial approach, following is propesed. first, Establishment of an adequate planting plan and development of slope stalilization method by planting native species. second, Sellection of adequate species by planting experiments for pioneer species, native species, and dietary species. third, Landscape planting zones should be managed artificially, the others be managed with ecological approach.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.39 no.2
• /
• pp.159-166
• /
• 2013

In order to develop new skin whitening agents, we prepared the EtOAc layer (AJE) after enzyme treatment of 75% EtOH extract of the Alnus Japonica Steud. We measured their tyrosinase inhibitory activity in vitro and melanin synthesis inhibitory activity in B16-F1 melanoma cells. They did not show inhibitory activity against mushroom tyrosinase but showed melanin synthesis inhibitory activity in a dose-dependent manner. In a melanin synthesis inhibition assay, AJE suppressed melanin production up to 52% at a concentration of $40{\mu}g/mL$. To elucidate the mechanism of the inhibitory effects of AJE on melanogenesis, we measured expression of melanogenesis-related proteins by the western blot assay. As a result, AJE suppressed the expression of tyrosinase related protein 1 (TRP-1) and microphthalmia associated transcription factor (MITF). Moreover, AJE increased the expression of phosphorylated extracellular signal-regulated kinase (p-ERK). These results conclude that ERK activation by AJE reduces melanin synthesis via MITF downregulation and is subsequent to the inhibition of TRP-1 expression. Therefore, we suggest that AJE could be used as active ingredients for skin whitening.

• The Korean Journal of Ecology
• /
• v.28 no.2
• /
• pp.99-103
• /
• 2005

Actual vegetation map drown with the scale 1 to 100 and Alnus japonica population in Ja-neup and Woong-neup of the Mujechi moor were described in order to monitor long-termly and preserve permanently, where is a very rare Molinietea moor and a legally protected area. A total of 3036 plots of 5m $\times$ 5m were surveyed during summer 1996. Thiessen polygons of 1491 alder trees were derived from the plot data. Actual vegetation map was illustrated by 6 cover types such as needle spike-rush type, moor-grass type, alder-moor type, eulalia type, oak forest type, and exposed site. Molinia grasses native to the moor and Miscanthus grasses alien to the moor are reciprocally dominant. The area of Molinia grasslands was rapidly in decline and alder population size was dramatically in increase in the moor, particularly in Woong-neup. In Molinietea moor preservation more attention should be focused on the regulation of a nutrient rich soil from forest road and fire.

• Korean Journal of Pharmacognosy
• /
• v.23 no.3
• /
• pp.132-136
• /
• 1992

The whole plants of Selaginella tamariscina, Orostachycis japonicus, the cortex of Ulmus mandshurica, and the wood of Alnus japonica have been used as folk medicine for treating cancer. The cytotoxic activity of these plants were tested using a calorimetric tetrazolium assay (MTT assay). S. tamariscina and A. japonica showed mild $IC_{50}$ value, comparing with O. japonicus and U. mandshurica. So, MeOH extracts of S. tamariscina and A. japonica were partitioned into $CHCl_3$, EtOAc and n-BuOH, successively. The $CHCl_3$, EtOAc and BuOH fractions of S. tamariscina and A. japonica showed low percent of survival against $P_{388}$ and $MKN_{45}$ cells respectively. To isolate active components, they were subjected to silica gel column chromatography. Compound I was obtained from EtOAc extracts of S. tamariscina and identified as amentoflavone by chemical and spectral analysis. Amentoflavone inhibited the survival of P388 cells dose dependently, while not clearly inhibited that of $MKN_{45}$ cells.

• Proceedings of the Korean Society of Toxicology Conference
• /
• 2005.05a
• /
• pp.208-208
• /
• 2005

• Journal of Plant Biology
• /
• v.29 no.2
• /
• pp.109-115
• /
• 1986

Actinorhizal plants being capable of fixing atmospheric nitrogen in symbiotic association with Frankia were surveyed for their root nodules, and the root nodules were collected for further studies. Three species of Alnus and Elaeagnus (A. hirsuta, a. firma, A. japonica; E. glabra, e. umbellata, E. macrophylla) and one species of Myrica (M. rubura) were confirmed to bear the root nodules of typical external shape. Morphological studies using light microscope and scanning electron microscope revealed the presence of vesicle clusters in the cortex region of the root. Two tentative endophyte colonies were isolated from the root nodule of A. hirsuta.

• Proceedings of the Korea Environmental Mutagen Society Conference
• /
• 2004.05a
• /
• pp.110-111
• /
• 2004

• Proceedings of the PSK Conference
• /
• 2003.04a
• /
• pp.141.2-141.2
• /
• 2003

Subendothelial accumulation of foam cells plays a key role in the initiation of atherosclerosis. These foam cells accumulate in fatty streaks that evolve to more complex fibrofatty or atheromatous plaques. Oxidized LDL may also be involved in atherogenesis by inducing smooth muscle cell proliferation and smooth muscle foam cell generation. (omitted)