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http://dx.doi.org/10.15230/SCSK.2013.39.2.159

The Inhibitory Effects of Alnus Japonica Steud. Extract on Melanogenesis  

Lee, Jun Young (Saimdang Cosmetics Co. Ltd. R&D Center)
Im, Kyung Ran (Saimdang Cosmetics Co. Ltd. R&D Center)
Jung, Taek Kyu (Saimdang Cosmetics Co. Ltd. R&D Center)
Yoon, Kyung-Sup (Saimdang Cosmetics Co. Ltd. R&D Center)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.39, no.2, 2013 , pp. 159-166 More about this Journal
Abstract
In order to develop new skin whitening agents, we prepared the EtOAc layer (AJE) after enzyme treatment of 75% EtOH extract of the Alnus Japonica Steud. We measured their tyrosinase inhibitory activity in vitro and melanin synthesis inhibitory activity in B16-F1 melanoma cells. They did not show inhibitory activity against mushroom tyrosinase but showed melanin synthesis inhibitory activity in a dose-dependent manner. In a melanin synthesis inhibition assay, AJE suppressed melanin production up to 52% at a concentration of $40{\mu}g/mL$. To elucidate the mechanism of the inhibitory effects of AJE on melanogenesis, we measured expression of melanogenesis-related proteins by the western blot assay. As a result, AJE suppressed the expression of tyrosinase related protein 1 (TRP-1) and microphthalmia associated transcription factor (MITF). Moreover, AJE increased the expression of phosphorylated extracellular signal-regulated kinase (p-ERK). These results conclude that ERK activation by AJE reduces melanin synthesis via MITF downregulation and is subsequent to the inhibition of TRP-1 expression. Therefore, we suggest that AJE could be used as active ingredients for skin whitening.
Keywords
Alnus Japonica Steud; melanogenesis; TRP-1; MITF; ERK activation;
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