• The Korean Journal of Food And Nutrition
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• v.27 no.6
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• pp.1014-1021
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• 2014

This study was performed to investigate the quality characteristics of the Yanggaeng with the addition of fermented aged black giant garlic paste (FABGGP) such as pH, reducing power, color, texture, total polyphenol, DPPH radical scavenging activity, and sensory acceptability. FABGGP was incorporated into Yanggaeng at 0, 3, 6, 9 and 12%(w/w) weight amounts based on the total weight of cooked white bean and FABGGP. pH decreased significantly with the increasing levels of FABGGP add. Reducing the sugar content increased as the amount of FABGGP increased. In term of color, lightness(L) and yellowness(b) decreased significantly but redness increased with increasing the levels of FABGGP. In the texture analysis, hardness, springiness and gumminess of FABGGP Yanggaeng were lower than those of the control. Total polyphenol content and 1,1-di-phenyl-1-picrylhydrazyl (DPPH) radical scavenging activity significantly increased as the FABGGP concentration increased in the formulation, The results of the sensory test showed that Yanggaeng with 6% FABGGP had the highest score in color, overall preference and chewiness. Based on these results, it is suggested that Yanggaeng with up to 6% added FABGGP can be developed as products without adverse effects on the sensory characteristics.

• Journal of Plant Biotechnology
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• v.32 no.1
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• pp.31-35
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• 2005

To obtain regeneration system of onion, we analyzed the effects of 2,4-D and BA concentration on the embryogenic callus induction from mature embryos. The highest embryogenic callus induction ratio was shown on MS medium (Murashie and Skoog 1962) containing $2.5\;\cal{mg/L}\;or\;5\;\cal{mg/L}$ picloram after mature embryos were placed on medium. When induced callus were cultured on half strength of MS medium containing $1\;\cal{mg/L}$ Kinetin, the highest shoot formation ratio was observed on MS medium containing $1\;{mg/L}$ 2,4-D and $1\;{mg/L}$ BA. Embryogenic callus were cultured in MS liquid medium containing $1\;\ccal{mg/L}$ of 2,4-D and $1\;\cal{mg/L}$ BA. The suspension cultured cell clumps could be mass propagated. Embryogenic callus were friable, but non-embryogenic callus included a lot of moisture, hence the identification between embryogenic and non-embryogenic callus as easily achieved. When embryogenic callus as cultured on half strength of MS medium containing $1\;\cal{mg/L}$ Kinetin, shoots were induced. The whole plantlet was obtained on rooting medium containing $0.5\;\cal{mg/}$ of NAA.

• Microbiology and Biotechnology Letters
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• v.33 no.4
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• pp.248-254
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• 2005

One hundred thirty nine bacteria were isolated from rotten onions collected from main producing districts, Chang-Nyung, Eui-Ryung, and Ham-Yang in Korea. The $18\%$ (25 strains) of bacterial isolates have carboxymethylcellulase (CMCase) activity and the $53\%$ (74 strains) have polygalacturonase (PGase) activity. Thirty one among randomly selected 45 strains of PGase producing bacteria have pathogenicity to onions. The isolates were classified into Pseudomonas sp. (18 strains), Bacillus sp. (11 strains), Yers-inia sp. (7 strains), and others (9 strains) on the basis of FAMEs patterns. Eighteen strains of Pseudomonas sp. were mainly divided into three cluster in the dendrogram and only the two clusters of them showed pathogenicity to onions. CMCase and PGase activities of Pseudomonas sp. weaker than those of Bacillus sp.. However, the pathogenicity of pseudomonas sp. to soften onions was stronger than that of Bacillus sp. Inoculation of $10^{2}$ cfu of Pseudomonas sp. gives rise to softening of onions. Pseudomonas sp. was identified as Pseudomonas gladioli by biochemical and physiological characteristics. P. gladioli is the first reported bacterium as a pathogen of onion in Korea. In low temperature, P. gladioli showed better growth and higher PGase activity than those of Bacillus sp. identified as Bacillus subtilis. And pH 9.0 is optimal pH for PGase activity of B. subtilis while that of P. gladioli is pH $5.0\∼6.0$ which is the acidity of onions. Taken together, P. gladioli may be a main pathogene of onion rot during the cold storage condition.

• Korean Journal of Environmental Agriculture
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• v.17 no.4
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• pp.346-351
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• 1998

To investigate the hormetic effects of the low dose ${\gamma}-ray$ radiation on the germination rate, Welsh onion (Allium fistulosum L. cv. Eunchun and cv. Sukchangwoidae) seeds were irradiated at the dose of $0.5\;{\sim}24.0$ Gy with the ${\gamma}-ray$ radiation (Co-60). The germination rate of 'Eunchun' cultivar increased about 10% in the low dose ${\gamma}-ray$ irradiation group compared with that of the control. In the 'Sukchangwoidae' cultivar, the germination rate of the 4 Gy irradiation group increased 40% more than that of the control. Broadly, it seemed that the hormetic effects of the low-dose ${\gamma}-ray$ radiation were taken more promisingly in the uncultivated soil than in the fertile soil. The germination rate from the paper towel and filter paper based cultivation increased 10% and 16% more, respectively, in the 1 Gy irradiation group than that in the control group. And the electric conductivities of the above groups supposed to be taken hormetic effects of the ${\gamma}-ray$ radiation were lower than those of the control group. From the above results, it is suggested that the low dose ${\gamma}-ray$ radiation ranged from 1 Gy to 10 Gy could have the hormetic effects on the germination rate related characters in Welsh onion seeds.

• Journal of Bio-Environment Control
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• v.9 no.4
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• pp.230-236
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• 2000

For extension of storability, onion bulbs produced in Cheju Island were exposed to ${\gamma}$-ray irradiation at a dosage of 0, 3, or 6Krad and stored in a natural cavern, or in low (2$^{\circ}C$) or ambient temperature storage condition. Sprouting began from approximately five months in storage, regardless of storage condition, and increased as the storage period increased. From early August, sprouting as high as 50% occurred in the natural storage cavern, and 20% in ambient temperature storage when bulbs were treated with 0 krad ${\gamma}$-ray. However, bulbs treated with 3 or 6krad ${\gamma}$-ray did not any sprouting until six months in storage. No significant weight loss was observed in all treatments until sprouting occurred from 40 days after the initiation of storage. Weight loss, however, increased remarkably after 40 days in storage, indicating that it was closely related to sprouting during storage. Bulbs stored at 2$^{\circ}C$ showed 5~10% rot, but were still edible. Percent rot reached to as high as 50% in bulbs unirradiated and stored at ambient temperatures after seven months, while it in irradiated bulbs reached to 5, 33, and 30% at 2$^{\circ}C$, in a natural cavern, and ambient temperatures, respectively. Sugar contents slightly increased in irradiated bulbs in the later storage period under ambient temperatures, while sugar contents in irradiated bulbs in the later storage period under ambient temperatures, while sugar contents in Allium vegetables generally decrease in later storage if unirradiated. Sulfur content slightly increased in irradiated bulbs in the early storage period and decreased gradually in all treatments in the later storage stage.

• Journal of the Korea Organic Resources Recycling Association
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• v.22 no.4
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• pp.87-94
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• 2014

Objective of this study was to investigate characteristics of inorganic components contained in liquid fertilizer produced using bone powder and rice bran by adding dry yeast and molasses. Addition of dry yeast to liquid fertilizer resulted in little change in pH, considerable increase in EC, and it showed high EC value compared to the control which has no additives. Also, it was appeared that the dry yeast-added treatment had higher at $2,936mg{\cdot}L^{-1}$ of $NH_4$-N concentration than the control which had $1,782mg{\cdot}L^{-1}$. In the other hand, addition of molasses resulted in low pH and slightly low EC, as compared to the control. $NH_4$-N concentration in the no added molasses treatment was $2,936mg{\cdot}L^{-1}$ higher than its molasses added treatment which had $2,378mg{\cdot}L^{-1}$. In conclusion, it was shown that addition of dry yeast to liquid fertilizer increased ammonium nitrogen concentration by accelerating nitrogen mineralization, while molasses has an effect of inhibiting nitrogen mineralization and enhancing the characteristics of fermentation. With application of organic liquid fertilizer containing bone powder and rice bran increased the fresh weight of Allium tuberosum.

• Journal of Nutrition and Health
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• v.23 no.2
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• pp.135-147
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• 1990

This study was devised to observe the cytotoxic activities of garlic extracts against various cancer cells, that is, murine leukemic lymphocyte(L1210 and P388) and human rectal(HRT-18) and colon cancer cells(HCT-48 and HT-29) in vitro, and murine ascitic tumor cell(S-180) in vivo. Each cell-line except S-180 was cultured in medium containing serial concentration of the garlic extract in vitro. Inhibitory effect n the growth rate of the cancer cells was stronger in extracts of petroleum ether than that of ethanol. A lipid soluble compound in the extracts of garlic was cytocidal to murine leukemic cells, human rectal and colon cancer cells in vitro. The growth rates of the cancer cells in medium containing garlic extracts were inhibited gradually to a significant degree in proportion to the increase of the extract concentration. The cytotoxic activity of garlic active fraction from TLC was about 2.3 times more potent than that of crude garlic extract, one unit of cytotoxic activity against L1210 cells being equivalent to 4.2$\mu\textrm{g}$ and 1.8$\mu\textrm{g}$ from the crude garlic and active fraction, respectively. The Rf value of the active fraction on silica-gel TLC was 0.18 in condition that petroleum ether/ethyl ether/acetic acid mixture(90:10:1, v/v/v) was used as a developing solvent. The survival times of mice inoculated with S-180 cells were extended about 1.5 to 2 times in the groups treated with garlic extract(through i.p. and oral administration) compared with their control group(no garlic extract treatment). Observations were carried out on S-180. Ethanol extracts of garlic injured markedly tumor cells within 3 hours after injection.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.82-82
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• 2018

산채는 주로 산지에서 자생하는 것을 채취하거나 자생지와 비슷한 환경의 임간에서 재배하여 수확을 한다. 본 시험은 차광처리를 통해 산지가 아닌 노지에서 산채 재배 가능성을 검토하고자 하였다. 대상작목은 개미취(Aster tataricus), 눈개승마(Aruncus dioicus var. kamtschaticus), 단풍취(Ainsliaea acerifolia), 박쥐나물(Parasenecio auricudatus var. matsumurana), 산마늘(Allium microdictyon), 어수리(Heracleum moellendorffii), 우산나물(Syneilesis palmata)이다. 시험작목을 전북 남원시 허브시험장(표고 500m) 포장에서 2017년 4월에 종묘를 정식하여 약 2년간 재배하였다. 고온기인 5월부터 9월까지 35%, 55%, 75%, 95% 차광정도를 달리하여 설치 한 후 시설 내의 기온, 상대습도 등 환경조건 및 재배작물의 고사율, 출현율, 생육특성 등을 조사하였다. 시설 내부의 평균 기온은 차광정도가 높아질수록 다소 낮아졌으며, 상대습도는 점차 높아졌다. 차광정도별 고사율을 조사한 결과 개미취, 어수리, 우산나물의 경우는 차광비율에 상관없이 각각 3.6%, 5.7%, 9.8%로 낮게 나타났다. 반면 단풍취와 산마늘의 경우 차광 비율에 따른 편차가 크지는 않았지만 최대 고사율이 각각 20.0%, 36.8%로 다소 높게 나타났다. 또한 눈개승마 75% 차광에서 50.0%, 박쥐나물 35% 차광에서 100% 고사율을 보여 적정 차광비율 선정 필요하다고 판단된다. 정식 2년차 4월 평균 출현율은 개미취 100%, 눈개승마 86.2%, 단풍취 84.3%, 박쥐나물 77.9%, 산마늘 97.5%, 어수리 88.9%, 우산나물 96.3%이었다. 다만 단풍취, 박쥐나물은 차광정도가 높아질수록 출현율이 높아졌고, 어수리는 차광정도가 높아질수록 다소 출현율이 낮아졌다. 또한 생육특성 조사 결과 초장은 단풍취를 제외하고 차광처리별 경향성이 뚜렷하게 나타나지 않았으며, 엽장과 엽폭은 산마늘을 제외한 작목들은 차광이 강할수록 커지는 경향이었다. SPAD 값은 개미취와 산마늘은 처리구역별 차이가 없었으나, 나머지 작물은 차광이 높아질수록 SPAD 값도 커졌다. 시험결과 표고 500m지역에서 개미취, 산마늘, 어수리, 우산나물은 차광수준에 따른 생육의 차이가 크지 않거나 차광이 낮아도 생육이 비교적 양호하였다. 그러나 단풍취와 박쥐나물은 차광정도에 따라 생육의 차이가 분명하였으며, 차광이 높아질수록 생육이 좋아졌다. 이를 바탕으로 산채류 노지재배를 위해서는 연차 간 작목별 생육특성, 수량성, 상품율, 경제적 분석 등의 추가적인 조사가 이루어져 할 것으로 여겨진다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.1
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• pp.84-88
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• 2011

Some thermally processed foods have higher biological activities due to their various chemical changes during heat treatment. Especially, 5-hydroxymethylfurfural (HMF) is derived from dehydration of sugars and has been identified in processed garlic. The biological function of HMF have revealed as antisickling agent and thyrosinase inhibitor. This study was carried out to examine the formation of HMF and free sugars from the aged garlics when it is treated at 60 and $75^{\circ}C$ and different incubation periods from 7 to 35 days. HMF and free sugars from the hot-water extracts of aged garlics were analyzed with GC/MS, LC/MS, and HPLC. The amount of HMF was higher than at $75^{\circ}C$ and increasing incubation period. Among free sugars, the only fructose except glucose and sucrose was formed and converted to HMF at high temperature and long incubation period. However, fructose formed in low temperature during making of aged garlic was rarely converted to HMF. This result indicates that formation of HMF can be dependent on the temperature and incubation period for making aged garlic.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.8
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• pp.972-978
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• 2008

Antioxidant compounds and activities were investigated for both northern (Uiseong) and southern ecotype (Namhae) domestic garlic stems. The contents of chlorophyll, carotenoid, vitamin C, phenolic compounds and allicin in garlic stems were measured. Antioxidant activities of methanol and ethanol extracts of garlic stems were compared determining lipid peroxidation on the linoleic acid system, electronic donating ability (EDA), superoxide dismutase (SOD)-like activity and nitrite scavenging ability (NSA). The amounts of chlorophyll, carotenoid, phenolic compounds and allicin in northern ecotype were significantly higher, but vitamin C content was lower than those of southern ecotype garlic stems. The allicin contents of garlic stems and garlic bulbs were $26.1{\pm}1.0{\sim}28.2{\pm}0.9$ and $33.2{\pm}0.7{\sim}33.5{\pm}0.8\;mg%$, respectively. All antioxidant activities were higher in methanol extracts of garlic stems than in ethanol extracts and were higher with northern ecotype than those with southern ecotype extract. The SOD-like activity of garlic stem extract was higher than that of garlic bulbs while EDA, lipid peroxidation and NDA of garlic stem extracts were lower than those of garlic bulbs. Antioxidant activities of garlic stems were more than 50% of garlic bulbs.