• 제목/요약/키워드: Alkaloids

검색결과 485건 처리시간 0.033초

맥각균(麥角菌)의 배양(培養)에 관한 연구(硏究)(IV) -한국산(韓國産) 맥각균(麥角菌)의 배양(培養)- (Studies on the Culture of Ergot Fungus(IV) -Culture of Korean Ergot Fungus-)

  • 김병각;심미자;최응칠;박영인
    • 생약학회지
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    • 제6권1호
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    • pp.9-13
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    • 1975
  • To isolate ergot strains which are capable of growing and producing alkaloids in submerged culture, strains were isolated from the sclerotia parasitizing the Graminae plants in Korea and the experiments of submerged culture of these strains yielded the following results: 1) The mycelia which were respectively isolated from the sclerotia parasitizing Agropyron semicostatum $N_{EES}$, Arundinella hirta $T_{ANAKA}$ var. ciliata $K_{OIDSUMI}$, Ischaemum anthephoroides $M_{IQ}$. var. eriostachyum $H_{ONDA}$, and Phleum pretense L. grew in Medium D by submerged culture. 2) When the strain of the ergot of Agropyron semicostatum was inoculated into six different nutrient solutions for submerged culture, its mycelium grew well in Media C, D and F, but produced alkaloids only in Medium C, indicating that Medium C is relatively suitable for the strain. 3) The extraction of the alkaloids from the culture broth by ether and T.L.C. analysis of the extract showed that it contained at least two types of alkaloids.

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Selection of Tropane Alkaloids High-Producing Lines by Single Cell Cloning of Hyoscyamus niger L. Root Cultures

  • Min, Ji-Yun;Park, Dong-Jin;Jeong, Mi-Jin;Song, Hyun-Jin;Kang, Seung-Mi;Kang, Young-Min;Choi, Myung-Suk
    • 한국산림과학회지
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    • 제98권2호
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    • pp.142-147
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    • 2009
  • Hyoscyamus species is sources of the hypnotic and sedative drugs hyoscyamine and scopolamine. Single cells of Hyoscyamus niger were dissociated from suspension cultures and adventitious roots obtained from single-cell clones which were cultured on B5 medium containing 3% (w/v) sucrose, 0.1 mg/L IBA and 0.4% (w/v) gelrite. H. niger adventitious root lines showed wide variation in tropane alkaloids production and growth. An effective selection of 200 root lines was made possible by the application of the 'Dragendorff's reagent' for qualitative detection of the alkaloids from root. A high correlation coefficient (r=0.9390) was observed between the values obtained with the two methods based on HPLC and Dragendorff's reagent analysis. Among the selected roots, the highest scopolamine content was 16.64 mg/g DW (Hn-59), which was 8.82-fold more productive than the lowest alkaloid producing line (Hn-25). Here, we established an efficient selection method on tropane alkaloids production and suggest that the Dragendorff's reagent is of great practical value in selection of invisible compounds.

Tryptophan-derived Alkaloids from Hedera rhombea Fruits and Their Butyrylcholinesterase Inhibitory Activity

  • Ha, Manh Tuan;Park, Se Eun;Kim, Jeong Ah;Woo, Mi Hee;Choi, Jae Sue;Min, Byung Sun
    • Natural Product Sciences
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    • 제28권3호
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    • pp.138-142
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    • 2022
  • Alzheimer's disease (AD) is the most common age-related neurodegenerative disease in industrialized countries. It is estimated that about 47 million people living with dementia and the number of cases will be tripled by 2050. However, the exact mechanism of AD is not known, and full therapy has still not been found. Various tryptophan-derived alkaloids have been reported as promising agents for the treatment of AD. In the present study, a series of tryptophan-derived alkaloids were isolated and characterized from the methanol extract of Hedera rhombea fruit. Based on the analysis of their observed and reported spectroscopic data, their structures were identified as N-[4'-hydroxy-(E)-cinnamoyl]-L-tryptophan (1), N-[3',4'-dihydroxy-(E)-cinnamoyl]-L-tryptophan (2), N-[4'-hydroxy-(E)-cinnamoyl]-L-tryptophan methyl ester (3), and N-[3',4'-dihydroxy-(E)-cinnamoyl]-L-tryptophan methyl ester (4). These compounds were screened for anti-Alzheimer activity via their inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes in vitro. As a result, compounds 3 and 4 showed moderate BChE inhibition with IC50 values of 86.9 and 78.4 μM, respectively, compared to those of the positive control [berberine (IC50 = 11.5 μM)]. However, all four compounds did not show significant inhibition of the AChE enzyme. This is the first time, the AChE and BChE inhibitory activities of these tryptophan-derived alkaloids were investigated and reported.

Quantitative Analysis and Enantiomeric Separation of Ephedra Alkaloids in Ma Huang Related Products by HPLC-DAD and UPLC-MS/MS

  • Kyoung-Moon Han;Jinwoo Hwang;Sun Hee Lee;Boreum Park;Hyungil Kim;Sun Young Baek
    • Natural Product Sciences
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    • 제28권4호
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    • pp.168-180
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    • 2022
  • Ephedra is a genus of the Ephedraceae family and is found in temperate regions, such as Central Asia and Europe. Among the various ephedra species, Ma Huang (Ephedra herb) is derived from the aerial parts of Ephedra sinica S tapf, Ephedra equisetina Bunge, and Ephedra intermedia Schrenk & C.A. Mey. Ma Huang contains various ephedra alkaloids, including (-)-ephedrine, (+)-pseudoephedrine, (-)-norephedrine, (+)-norpseudoephedrine, (-)-methylephedrine, and (+)-methylpseudoephedrine, which are found naturally as single enantiomers, although they can be prepared as racemates. Although the use of Ma Huang in foods is prohibited in Korea, products containing Ma Huang can be imported, and so it is necessary to develop a suitable analytical technique for the detection of Ma Huang in foods. Herein, we report the development of analytical methods for the detection of ephedra alkaloids in products containing Ma Huang. Following sample purification by solid phase extraction, quantitative analysis was performed using ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS). Additionally, the enantiomers were successfully separated using HPLC-DAD. We successfully analyzed various food samples, where the ephedra alkaloids were qualitatively and quantitatively determined, and the enantiomers were separated. It is expected that these methods may contribute toward preventing the distribution of illegal products containing Ma Huang.

Isoflavonoids and Alkaloids from Spartidium saharae

  • Abdel-Halim, Osama B.;Abdel-Fattah, Hosny A.;Halaweish, Fathi T.;Halim, Ahmed F.
    • Natural Product Sciences
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    • 제6권4호
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    • pp.189-192
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    • 2000
  • A new isoflavone, $(+)-4'-O-methyl-8-C-{\beta}-D-glucopyranosylgenistein$, was isolated from the aerial parts of Spartidium saharae together with the known isoflavone, $(+)-8-C-{\beta}-D-glucopyranosylgenistein$ as well as dipiperidine alkaloids, (+)-ammodendrine and (+)-N-acetyl hystrine. Details of their structure elucidation are based on chemical and spectroscopic methods. N-formyl ammodendrine was detected by GC-MS. The potential chemotaxonomic value of the alkaloid content is explored. Cytotoxic activity has been determined for both alcoholic extract and isolated compounds.

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자생식물 미치광이풀의 모상근배양에 의한 Tropane alkaloids 생산

  • 정희영;강민정;강영민;강승미;박우진;윤대진;박정동;최명석
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2001년도 추계학술발표대회
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    • pp.483-486
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    • 2001
  • Growth and tropane alkaloids(hyoscyamine and scopolamine) contents were studied in root cultures from Scopolia paruiflora. All excised roots grew well, even without an addition of a growth regulators. Scopolia roots could be divided scopolamine-rich species. Among the culture media, SH medium was the best for tropane alkaloids production from the hairy roots, whereas the growth increased in SH medium. In precusor experiments, feeding of hyoscyamine was promotive for scopolamine formation. Abiotic elicitors made a slightly production promotion effect.

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세잎돌쩌기의 알칼로이드 성분과 함량의 계절적 변화 (Alkaloid Constituents of Aconitum triphyllum NAKAI and their Seasonal Variation)

  • 이진우;박종희;김혜경;이정규
    • 생약학회지
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    • 제35권2호통권137호
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    • pp.128-133
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    • 2004
  • Aconitum triphyllum Nakai, the Korean spontaneous perennial herb, is one of the most important materials of oriental drug Fu-zi(aconite) and has long been used as cardiotonic for arrhythmia, analgesic and antiinflammatory drug in oriental countries. Although the plant is widely used for the preparation of aconite, the composition and amount of the toxic aconitine type alkaloids have not studied so far. As the preliminary study for seasonal variation of major constituents in mother tuber and daughter tuber of the plant, the authors tried to elucidate phytochemical characteristics of $C_{19}-diterpenoid alkaloids by $^{13}C-NMR$ spectra and seasonal variation of the alkaloidal contents by high performance liquid chromatography.

In Vivo Disease Control Efficacy of Isoquinoline Alkaloids Isolated from Corydalis ternata against Wheat Leaf Rust and Pepper Anthracnose

  • Han, Jae Woo;Shim, Sang Hee;Jang, Kyoung Soo;Choi, Yong Ho;Kim, Hun;Choi, Gyung Ja
    • Journal of Microbiology and Biotechnology
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    • 제28권2호
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    • pp.262-266
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    • 2018
  • Phytochemicals have been considered as alternatives for synthetic fungicides because of their biodegradability and low toxicity. In this study, we found that the methanolic extract of Corydalis ternata suppressed the development of plant diseases caused by Puccinia triticina and Colletotrichum coccodes. As the antifungal substance, three isoquinoline alkaloids (dehydrocorydaline, stylopine, and corydaline) were isolated from C. ternata. These active compounds also exhibited in vivo antifungal activity against P. triticina and C. coccodes. Taken together, our results suggest that C. ternata and its active compounds can be used to control plant diseases.

Analysis of Alkaloids in the Seeds of Zizyphus jujuba by High Performance Liquid Chromatography

  • Park, Man-Ki;Park, Jeong-Hill;Shin, Young-Geun;Cho, Kyung-Hee;Han, Byung-Hoon;Park, Myung-Hwan
    • Archives of Pharmacal Research
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    • 제14권2호
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    • pp.99-102
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    • 1991
  • A hight performance liquid chromatographic methods was developed for the seperation and determination of seven alkaloids in "sanjoin" (the seeds of Zizyphus jujuba Rhamnaceae), a plant with potent sedative activity. A reverse phase system of Lichrosorb RP-Select B column and 0.05 M potassium phosphate buffer (pH = 3.5)-acetonitrile with gradient elution was employed. Two known alkaloids, juzirine and lysicamine, were newly isolated fom "sanjoin"."sanjoin".oin&".ot;.

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경포부자의 다이테르펜 알칼로이드 성분 (Diterpene Alkaloids from Kyong-Po Buja (Processed Aconitium carmichaeli))

  • 최상진;권학철;민용득;이성옥;김경란;최상운;손건호;강삼식;이강노
    • 생약학회지
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    • 제33권3호통권130호
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    • pp.187-190
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    • 2002
  • Three diterpene alkaloids, songorine (1), neoline (2), N-deethylneoline (3), were isolated from Kyong-Po Buja (Processed Aconitium carmichaeli, Ranunculaceae) which was used as Chinese traditional medicine with analgesic, anti-inflammatory and cardiotonic activity. Their structures were established by chemical and spectroscopic methods.