• 한국동물학회지
• /
• 제36권3호
• /
• pp.425-432
• /
• 1993

Culex pipiens pallens에 존재하는 Alkaline phosphatase의 연구를 위한 적정 분석조건과 우화 후, 시간 경과에 따른 Alkaline phosphatase 활성 경향에 대해서 연구하였다. C. pipiens에 존재하는 Alkaline phosphatase의 활성은 우화 직후부터 지속적으로 감소하다가, 흡혈 자극에 의해서 급격하게 증가한다. 흡혈 후 30시간이 경과했을 때, 최대의 활성도를 보이고 감소하나, 흡혈 48시간 이후에는 다시 증가하여 지속적으로 유지됨을 알 수 있다. 기관별 분석에서 첫번째 활성 증가는 중장에서 일어나고, 두번째 활성 증가는 난소에서 일어남을 알 수 있다. 그리고 흡혈 후 30시간된 성체에서는 5개의 동위효소 밴드가 보이는데, 난소에서 ALP-1와 ALP-2가 나타나고, 가슴에서는 ALP-3, ALP-4와 ALP-5가 보인다. 지방체에서는 ALP-4와 ALP-5가, 중장에서는 ALP-3, ALP-4와 ALP-5가 나타남을 알 수 있다. 그리고 흡혈 후 72시간된 성체에서, ALP-1, ALP-2가 동일하게 존재함을 알 수 있다.

• Mass Spectrometry Letters
• /
• 제9권3호
• /
• pp.67-72
• /
• 2018

Alkaline phosphatase (AP) is a membrane-bound glycoprotein that is widely distributed in the plasma membrane of cells of various organs and also found in many organisms from bacteria to humans. The complete amino acid sequence and three-dimensional structure of human placental alkaline phosphatase have been reported. Based on the literature data, AP consists of two presumptive glycosylation sites, at Asn-144 and Asn-271. However, it only contains a single occupied N-linked glycosylation site and no occupied O-linked glycosylation sites. Hydrophilic interaction chromatography (HILIC) has been primarily employed for the characterization of the glycan structures derived from glycoproteins. N-glycan structures from human placental alkaline phosphatase (PLAP) were investigated using HILIC-Orbitrap MS, and subsequent data processing and glycan assignment software. 16 structures including 10 sialylated N-glycans were identified from PLAP.

• 대한수의학회지
• /
• 제11권2호
• /
• pp.141-143
• /
• 1971

The serum alkaline phosphatase activity was determined with 64 Korean cattles in different sexe and age groups, using p-nitrophenylphosphate as the substrate. The results obtained were as follows: 1. The mean value of serum alkaline phosphatase activity in Korean cattle was $1.78{\pm}0.21$ Bessey-Lowry units/ml, and $1.71{\pm}0.21$ units/ml. in female and $1.86{\pm}0.21$ units/ml. in male, respectively. 2. The serum alkaline phosphatase activity decreased with age from 4 years group. 3. No statistical significance was recognized among the sexes, but difference among the age groups was found to be highly significant.

• 대한수의학회지
• /
• 제15권2호
• /
• pp.233-240
• /
• 1975

Glycogen and alkaline phosphatase were studied histochemically in the placenta of the golden hamster (Mesocricetus auratus). The results were summarized as followings: 1. The histochemical distribution of glycogen and alkaline phosphatase was described in the various cells of both the placenta and uterus from implantation (5 days past coitum) parturition. 2. Glycogen appeared in the extraembryonic ectorm of the implanting ovum and increased gradually in the ectoplacental cone with the development of the placenta. 3. Glycogen granules began to accumulate in the labylinthine trophoblast of chorioallantoic placenta at 10.5 days and in the endodermal cells the visceral yolk sac sac at 10 days, respectively, and in these both cells glycogen increased gradually until the 13 days post coitum and after this time decreased progressively. 4. Alkaline phosphatase activity was high in the labyrinth, junctional zone and decidua, but was not observed in the yolk sac and trophospongium.

• 한국미생물·생명공학회지
• /
• 제13권4호
• /
• pp.403-408
• /
• 1985

Rhizopus oryzae를 액체배양하는 동안, $\alpha$-amylase, acid phosphatase 그리고 alkaline phosphatase와 같은 분비효소의 분비능에 대한 계면활성 제의 촉진효과를 조사하였다. 그 결과 집락의 형태적인 변화를 볼 수 있었는바, 0.18mM SDS 처리구에서는 작은 pelletal형, 0. 48mM DOC 처리구에서는 pulpy형, 계면활성제를 처리하지 않은 시험구에서는 사상형의 집 락을 나타내었다. SDS를 첨가함으로써 $\alpha$-amylase는 9배, acid phosphatase는 25배의 활성도 증가가 유도되었다. DOC첨가 시험구에서 alkaline phosphatase 활성도는 배양액에서 11배 증가가 나타났을 뿐 아니라 세포질에서도 역시 상승하였다.

• 동아시아식생활학회지
• /
• 제23권1호
• /
• pp.39-43
• /
• 2013

Carthamus tinctorius L. and Eucommia umoides Oliver are often used in traditional herbal medicines for reducing damage to the liver, kidney, bone and muscle. In the present study, we investigated cell viability and alkaline phosphatase activity in the human osteoblast-like MG-63 cell line with methanol extracts of Carthami Semen (CS) and Eucommiae Cortex (EC) alone or in a mixture (CS+EC). Osteoblast cell viability was evaluated using the MTS assay and alkaline phosphatase activity assays. The cell viability and alkaline phosphatase activity significantly increased in MG-63 osteoblast cells treated with the CS+EC mixture. These findings suggest the CS+EC mixture may have beneficial effects on bone health through the proliferation of osteoblast cells.

• 한국동물학회지
• /
• 제16권2호
• /
• pp.139-145
• /
• 1973

솔나방(Dendrolimus spectabilis)의 變態에 따른 acid 및 alkaline phosphatase의 活性度의 變化를 測定하였다. 두 酵素의 活性度는 幼蟲의 成長과 더불어 增加하고 전용기에서 減少하였다. Acid phosphatase는 용기의 初에, 그리고 alkaline phosphatase는 幼蟲8齡에서 각각 最高의 活性을 나타내었다. 전용기에서 두 酵素간의 活性度의 차이는 크게 나타나지 않았으나 幼蟲8齡에서 보다는 훨씬 낮았다. Acid phosphatase의 活性度는 용기초에 增加하고 용기말에서 減少하다가 成蟲에서 다시 上昇하였다. Total phosphatase의 活性度의 變化는 變態에 따른 生理的曲線인 U字型 pattern을 나타내었다.

• 약학회지
• /
• 제37권6호
• /
• pp.571-576
• /
• 1993

The substrate specificity of the purified rabbit plasma alkaline phosphatase (ALPase) was determined towards a extended range of potential substrates including relatively simple phosphate derivatives as p-NPP and indolyl phosphate, and several synthetic peptides and phosphoproteins. These results further estabilish the broad substrate specificity of these circulating enzymes. Interestingly, the plasma ALPase preferentially dephosphorylates Thr over Ser residues, as demonstrated with a series of synthetic peptides. The latter result is in contradiction to the behaviour of the tissue ALPase, which is thought to the ultimate source of plasma ALPase, and open therefore new perspectives with respective to the origin and "solubilisation" processes of these enzymes. Dephsphrylation of protein substrates by endogenous and isolated plasma ALPases indicates that ALPase probably displays protein phosphatase activity in vivo.

• 한국균학회지
• /
• 제25권2호통권81호
• /
• pp.85-90
• /
• 1997

표고버섯(Lentinula edodes)의 동위효소 양상을 표고 연구의 기초 연구의 일환으로 실시하였다. 균사체의 Tris-HCl 완충액에 용해되는 세포내 효소를 nondenaturing polyacrylamide gel 전기영동 법으로 분리후, peroxidase, esterase, superoxide dismutase, acid phosphatase, alkaline phosphatase, alcohol dehydrogenase, ${\alpha}-amylase$ 효소 활성을 측정하였다. 표고는 peroxidase, esterase, superoxide dismutase, acid phosphatase 활성이 검출되었으며, 본 연구에서 사용한 방법으로 alkaline phosphatase, alcohol dehydrogenase, ${\alpha}-amylase$ 효소 활성은 검출되지 않았다. 표고버섯의 peroxidase, esterase band는 배양 기간에 따라 큰 차이가 없이 안정하였으며, 동위효소는 표고의 유전적 연구 및 원형질체 융합 후 융합체의 특성 연구에 중요하다.

• Ocean Science Journal
• /
• 제40권4호
• /
• pp.183-190
• /
• 2005

We investigated the seasonal variability of tree alkaline phosphatase activity in seawater and alkaline phosphatase hydrolysable phosphorus (APHP) at 3 stations in Hiroshima Bay using alkaline phosphatase extracted from the dinoflagellates Alexandrium tamarense and Gymnodinium catenatum. The dissolved inorganic phosphorus (DIP) was lower than $1\;{\mu}M$ all samples; the lowest values were in May. The amount of APHP was high at the surface and bottom waters of all stations in May, showing DIP-depleted conditions. In August and November, the amount of APHP was much less than the amount of APHP in May, indicating that the availability of dissolved organic phosphorus (DOP) for these species was low and/or uptake during the dinoflagellate blooming might have occurred in the area. The results obtained from short-term variations of AP activity might suggest that the growth of dinoflagellates in this season may be partly supported by the AP produced by other diatoms.