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Alkaline Phosphatase Activity and Phosphatase Hydrolyzable Phosphorus for Phytoplankton in Hiroshima Bay, Japan  

Oh, Seok-Jin (Division of Bioresource and Bioenvironmental Science, Kyushu University Graduate School)
Yoon, Yang-Ho (Division of Ocean System, College of Fisheries and Ocean Sciences, Yosu National University)
Yamamoto, Tamiji (Graduate School of Biological Sciences, Hiroshima University)
Matsuyama, Yukihiko (Hamful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea)
Publication Information
Ocean Science Journal / v.40, no.4, 2005 , pp. 183-190 More about this Journal
Abstract
We investigated the seasonal variability of tree alkaline phosphatase activity in seawater and alkaline phosphatase hydrolysable phosphorus (APHP) at 3 stations in Hiroshima Bay using alkaline phosphatase extracted from the dinoflagellates Alexandrium tamarense and Gymnodinium catenatum. The dissolved inorganic phosphorus (DIP) was lower than $1\;{\mu}M$ all samples; the lowest values were in May. The amount of APHP was high at the surface and bottom waters of all stations in May, showing DIP-depleted conditions. In August and November, the amount of APHP was much less than the amount of APHP in May, indicating that the availability of dissolved organic phosphorus (DOP) for these species was low and/or uptake during the dinoflagellate blooming might have occurred in the area. The results obtained from short-term variations of AP activity might suggest that the growth of dinoflagellates in this season may be partly supported by the AP produced by other diatoms.
Keywords
Alkaline phosphatase; Alexandrium tamarense; Gymnodinium catenatuin; DIP; DOP; APHP;
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1 Hiroshima Prefecture. 1995. Report to observation of shellfish poisoning, 1994. Hiroshima, 1-5. (In Japanese)
2 Holm-Hansen, O.C., C.J. Lorenzen, R.W. Holms, and J.D.H. Strickland. 1965. Fluorometric determination of chlorophyll. J. Cons. Perm. Int. Explor. Mer., 30, 3-15
3 Kuenzler, E.J. and J.P. Perras. 1965. Phosphatases of marine algae. Bull. Mar. Biol. Lab., 128, 271-284   DOI   ScienceOn
4 Kuenzler, E.J. 1965. Glucose-6-phosphate utilization by marine algae. J. Phycol., 1, 156-164   DOI
5 SECA (Seto Inland Sea Environmental Conservation Association). 1998. Seto Inland Sea Environmental Conservation, Kobe. 1-17. (In Japanese)
6 Tada, K., K. Monaka, M. Morishita, and T. Hashimoto. 1998. Standing stocks and production rates of phytoplankton and abundance of bacteria in the Seto Inland Sea, Japan. J. Oceanogr., 54, 285-295   DOI   ScienceOn
7 Valiela, I. 1995. Marine Ecological Processes. Springer-Verlag, New York. 686 p
8 Yamamoto, T., M. Ishida, and T. Seiki. 2002a. Long-term variation in phosphorus and nitrogen concentration in the Ohta River water, Hiroshima, Japan as a major factor causing the change in phytoplankton species composition. Bull. Jpn. Soc. Fish. Oceanogr., 66, 102-109. (In Japanese)
9 Gonzalez-Gil, S., B. A. Keafer, R.V.M. Jovine, A. Aguilera, S. Lu, and D.M. Anderson. 1998. Detection and quantification of alkaline phosphatase in single cells of phosphorus-starved marine phytoplankton. Mar. Ecol. Prog. Ser., 164, 21-35   DOI
10 Chrost, R.J. 1991. Microbial enzymes in aquatic environments. Springer-Verlag, New York. 317 p
11 Koroleff, F. 1983. Determination of phosphorus. P. 172. In: Methods of seawater analysis. ed. by K. Grasshoff, M. Ehrhardt, and K. Kremling. Verlag Chemie, Weinheim
12 Cembella, A.D., N.J. Antia, and P.J. Harrison. 1984. The utilization of inorganic and organic phosphorus compounds as nutrients by eukaryotic microalgae: A multidisciplinary perspective: Part 1. CRC Critic. Rev. Microbiol., 10, 317-391
13 Keller, M.D., R.C. Selvin, W. Claus, and R.R.L. Guillard. 1987. Media for the culture of oceanic ultraphytoplankton. J. Phycol., 23, 633-638   DOI
14 Hernandez, I. and B.A. Whitton. 1996. Retention of P-nitrophenol and 4-methylumbelliferone by marine macroalgae and implications for measurement of alkaline phosphatase activity. J. Phycol., 32, 819-825   DOI   ScienceOn
15 Oh, S.J., T. Yamamoto, Y. Kataoka, O. Matsuda, Y. Matsuyama, and Y. Kotani. 2002. Utilization of dissolved organic phosphorus by the two toxic dinoflagellates, Alexandrium tamarense and Gymnodinium catenatum (Dinophyceae). Fisheries Sci., 68, 416-424   DOI   ScienceOn
16 Oh, S.J. and Y.H. Yoon. 2004. Effects of water temperature, salinity and irradiance on the growth of the toxic dinoflagellate, Gymnodinium catenatum (Graham) isolated from Yeosuhae Bay, Korea. Algae., 19, 1-10. (In Korean)   DOI
17 Pollehne, F., S. Busch, G. Jost, B. Meyer-Harms, M. Nausch, M. Reckermann, P. Schaning, D. Setzkorn, N. Wasmund, and Z. Witek. 1995. Primary production patterns and heterotrophic use of organic material in the Pomeranian Bay (Southern Baltic). Bull. Sea. Fish Inst., 3, 43-60
18 Strickland, J.D.H. and T.R. Parsons. 1972. A practical handbook of seawater analysis. Fishries Research Board of Canada, Ottawa. 310 p
19 Suzumura, M., K. Ishikawa, and H. Ogawa. 1998. Characterization of dissolved organic phosphorus in coastal seawater using ultrafiltration and phosphohydrolytic enzymes. Limnol. Oceanogr., 43, 1553-1564   DOI   ScienceOn
20 Yamaguchi, M. and S. Itakura. 1999. Nutrition and growth kinetics in nitrogen- or phosphorus-limited cultures of the noxious red tide dinoflagellate Gymnodinium mikimotoi. Fisheries Sci., 65, 367-373
21 Maeda, M. and N. Taga. 1973. Deoxyribonuclease activity in seawater and sediment. Mar. Biol., 20, 58-63   DOI
22 Uchida, T. 1992. Alkaline phosphatase and nitrate reductase activity in Prorocentrum micans Ehrenberg. Bull. Plankton Soc. Jpn., 38, 85-92
23 Hallegraeff, G.M., S.O. Stanley, C.J. Bolch. and S.I. Blackburn. 1989. Gymnodinium catenatum blooms and shellfish toxicity in southern Tasmania, Australia. p. 77-80. In: Red tides. ed. by T. Okaichi, D.M. Anderson, and T. Nemoto. Elsevier, New York
24 Parsons, T.R., M. Takahashi, and B. Margrave. 1984. Biological oceanographic processes. Pergamon Press. 330 p
25 Guillard, R.R.L. and P.E. Hargraves. 1993. Stichochrysis immobilis is a diatom, not a chrysophyte. Phycologia, 32, 234-236   DOI
26 Yamamoto, T. and K. Tarutani. 1997. Effects of temperature, salinity and irradiance on the growth of toxic dinoflagellate Alexandrium tamarense isolated from Hiroshima Bay, Japan. Jpn. J. Phycol. (Sorui), 45, 95-101. (In Japanese)
27 Yamamoto, T., S.J. Oh, and Y. Kataoka. 2002b. Effect of temperature, salinity and irradiance on the growth of the toxic dinoflagellate Gymnodinium catenatum (Dinophyceae) isolated from Hiroshima Bay, Japan. Fisheries Sci., 68, 356-363   DOI   ScienceOn
28 Nausch, M. 1998. Alkaline phosphatase activities and the relationship to inorganic phosphate in the Pomeranian Bight (southern Baltic Sea). Aquat. Microb. Ecol., 16, 87-94   DOI   ScienceOn
29 Boni, L., E. Carpene, D. Wynne, and M. Reti. 1989. Alkaline phosphatase activity in Protogonyaulax tamarensis. J. Plankton. Res., 11, 879-885   DOI