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PCR-based markers to select plastid genotypes of Solanum acaule (Solanum acaule 색소체 유전자형 선발을 위한 특이적 분자마커 개발)

  • Park, Tae-Ho
    • Journal of Plant Biotechnology
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    • v.49 no.3
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    • pp.178-186
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    • 2022
  • The tetraploid Solanum acaule is a wild potato species from Bolivia widely used for potato breeding because of its diverse attractive traits, including resistance to frost, late blight, potato virus X, potato virus Y, potato leafroll virus, potato spindle tuber viroid, and cyst nematode. However, the introgression of useful traits into cultivated potatoes via crossing has been limited by differences in endosperm balance number between species. Somatic fusion could be used to overcome sexual reproduction barriers and the development of molecular markers is essential to select proper fusion products. The chloroplast genome of S. acaule was sequenced using next-generation sequencing technology and specific markers for S. acaule were developed by comparing the obtained sequence with those of seven other Solanum species. The total length of the chloroplast genome is 155,570 bp, and 158 genes were annotated. Structure and gene content were very similar to other Solanum species and maximum likelihood phylogenetic analysis with 12 other species belonging to the Solanaceae family revealed that S. acaule is very closely related to other Solanum species. Sequence alignment with the chloroplast genome of seven other Solanum species revealed four InDels and 79 SNPs specific to S. acaule. Based on these InDel and SNP regions, one SCAR marker and one CAPS marker were developed to discriminate S. acaule from other Solanum species. These results will aid in exploring evolutionary aspects of Solanum species and accelerating potato breeding using S. acaule.

The Importance of Employee's Perceptions When Conducting a Company's CSR Strategy : The Concept of 'Authenticity' (조직의 CSR 전략 이행과정에서 직원 인식 중요성 : '진정성' 개념을 바탕으로)

  • Jung, Ji-Young;Kim, Sang-Joon
    • Korean small business review
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    • v.43 no.4
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    • pp.27-57
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    • 2021
  • How does authenticity influence the process that conducts a company's CSR Strategy? Authenticity, an internal/external alignment condition that an employee feels in relation to an organization, means the decision on how true and beneficial to employees through their experiences, such as thoughts and emotions. Also, it can be understood as a process of meaning formation between the organization's strategy to conduct CSR and the perception of employees conducting CSR. To prove the relation between authenticity and CSR clearly, we used various techniques like Text Mining, Topic Modeling and Semantic network analysis about O corporation's 657 review data, from 2015 to 2021. As a result of the analysis, we find out the special issues and types. The analysis shows that the issue concerning the 'external image' is the biggest characteristic of authenticity perception in other conditions. Furthermore, the types of authenticity perception evaluations are largely divided into acceptance and rejection, in detail, five categories. This study indicates that organizations should consider both external and internal conditions when establishing CSR strategies. In addition, it is necessary to be an interactive circular relationship between the organization and employee, collecting and reflecting employee's perceptions. Finally, this study proposes ways to overcome problems related to interaction.

Noise Measurement by Percentage of Effective Linear Attenuation Coefficient of Water in CT Image of AAPM CT Performance Phantom (AAPM CT 성능 팬텀의 CT영상에서 물 유효선감쇠계수의 백분율에 의한 노이즈 측정)

  • Jong-Eon, Kim;Sang-Hun, Lee
    • Journal of the Korean Society of Radiology
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    • v.16 no.6
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    • pp.771-778
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    • 2022
  • The purpose of this study is to present a method of measuring noise by the percentage of effective line attenuation coefficient of water that can be used for quality control of CT image noise using AAPM CT performance phantom in clinical practice. In the CT images obtained by scanning the AAPM CT performance phantom with a 120 kVp CT X-ray beam, the mean CT number was measured for each pin and water in the CT number linearity insert part. The effective energy was determined as the photon energy with the largest correlation coefficient from the correlation coefficients of the linear regression analysis of the measured mean CT number for each pin and water and the linear attenuation coefficient for each photon energy. And for water and acrylic, the contrast scale was calculated as 0.000188 cm-1 · HU-1 from the measured mean CT number and effective line attenuation coefficient. Using the calculated contrast scale, the effective line attenuation coefficient of water, and the standard deviation measured in the water of the alignment pin part of the AAPM CT performance phantom, The noise measurement value by the percentage of effective line attenuation coefficient of water obtained 0.31 ~ 0.52% in the range of 100 ~ 300 mAs.

One-point versus two-point fixation in the management of zygoma complex fractures

  • Lee, Kyung Suk;Do, Gi Cheol;Shin, Jae Bong;Kim, Min Hyung;Kim, Jun Sik;Kim, Nam Gyun
    • Archives of Craniofacial Surgery
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    • v.23 no.4
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    • pp.171-177
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    • 2022
  • Background: The treatment of zygoma complex fractures is of crucial importance in the field of plastic surgery. However, surgical methods to correct zygoma complex fractures, including the number of fixation sites, differ among operators. Although several studies have compared two-point and three-point fixation, no comparative research has yet been conducted on one-point versus two-point fixation using computed tomography scans of surgical results. Therefore, the present study aimed to address this gap in the literature by comparing surgical results between one-point and two-point fixation procedures. Methods: In this study, we randomly selected patients to undergo surgery using one of two surgical methods. We analyzed patients with unilateral zygoma complex fractures unaccompanied by other fractures according to whether they underwent one-point fixation of the zygomaticomaxillary buttress or two-point fixation of the zygomaticomaxillary buttress and the zygomaticofrontal suture. We then made measurements at three points-the zygomaticofrontal suture, inferior orbital wall, and malar height-using 3-month postoperative computed tomography images and performed statistical analyses to compare the results of the two methods. Results: All three measurements (zygomaticofrontal suture, inferior orbital wall, and malar height) showed significant differences (p< 0.05) between one-point and two-point fixation. Highly significant differences were found for the zygomaticofrontal suture and malar height parameters. The difference in the inferior wall measurements was less meaningful, even though it also reached statistical significance. Conclusion: Using three parameters in a statistical analysis of imaging findings, this study demonstrated significant differences in treatment outcomes according to the number of fixations. The results indicate that bone alignment and continuity can be achieved to a greater extent by two-point fixation instead of one-point fixation.

Improvement of Silkworm Egg Microinjection Using 3D Printing Technology (3D 프린팅 기술을 이용한 누에 알 미세주입 기술 개선)

  • Jeong, Chan Young;Lee, Chang Hoon;Seok, Young-Seek;Yong, Sang Yeop;Kim, Seong-Wan;Kim, Kee Young;Park, Jong Woo
    • Korean journal of applied entomology
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    • v.61 no.1
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    • pp.249-254
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    • 2022
  • Silkworms, which have for long been used as an insect resource for industrialization, have recently attracted attention as potential bio-factories for the production of novel biomaterials. In this regard, material production is typically achieved based on transformation technology, mediated via microinjection, in which a target gene is inserted into eggs containing an embryo. However, an essential step in the microinjection procedure is egg fixation, which can be a time-consuming and laborious task. Therefore, in this study, using the 3DCADian program, we adopted a 3D printing approach to model egg liners and glue drawers, which can contribute to facilitating egg alignment and fixation, thereby enhancing transformation efficiency by reducing time consumption and fatigue. After rendering using Fusion 360, the two supplementary tools were produced by printing with nylon resin (PA12) and Sinterit Lisa Pro. Subsequent analysis of the time required to fix eggs on glass slides using the two manufactured tools, revealed that the processing time was reduced by approximately 18.6% when the two tools were used compared with when these tools were not used. These innovations not only reduced fatigue but also contributed to more effective use of the microscope and manipulator for microinjection. Consequently, we believe that with additional research and refinement, the egg liner and glue drawer developed in this study could be used to enhance silkworm transformation efficiency and study similar transformation systems in other industrial insects.

Transferring Calibrations Between on Farm Whole Grain NIR Analysers

  • Clancy, Phillip J.
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1210-1210
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    • 2001
  • On farm analysis of protein, moisture and oil in cereals and oil seeds is quickly being adopted by Australian farmers. The benefits of being able to measure protein and oil in grains and oil seeds are several : $\square$ Optimize crop payments $\square$ Monitor effects of fertilization $\square$ Blend on farm to meet market requirements $\square$ Off farm marketing - sell crop with load by load analysis However farmers are not NIR spectroscopists and the process of calibrating instruments has to the duty of the supplier. With the potential number of On Farm analyser being in the thousands, then the task of calibrating each instrument would be impossible, let alone the problems encountered with updating calibrations from season to season. As such, NIR technology Australia has developed a mechanism for \ulcorner\ulcorner\ulcorner their range of Cropscan 2000G NIR analysers so that a single calibration can be transferred from the master instrument to every slave instrument. Whole grain analysis has been developed over the last 10 years using Near Infrared Transmission through a sample of grain with a pathlength varying from 5-30mm. A continuous spectrum from 800-1100nm is the optimal wavelength coverage fro these applications and a grating based spectrophotometer has proven to provide the best means of producing this spectrum. The most important aspect of standardizing NIB instruments is to duplicate the spectral information. The task is to align spectrum from the slave instruments to the master instrument in terms of wavelength positioning and then to adjust the spectral response at each wavelength in order that the slave instruments mimic the master instrument. The Cropscan 2000G and 2000B Whole Grain Analyser use flat field spectrographs to produce a spectrum from 720-1100nm and a silicon photodiode array detector to collect the spectrum at approximately 10nm intervals. The concave holographic gratings used in the flat field spectrographs are produced by a process of photo lithography. As such each grating is an exact replica of the original. To align wavelengths in these instruments, NIR wheat sample scanned on the master and the slave instruments provides three check points in the spectrum to make a more exact alignment. Once the wavelengths are matched then many samples of wheat, approximately 10, exhibiting absorbances from 2 to 4.5 Abu, are scanned on the master and then on each slave. Using a simple linear regression technique, a slope and bias adjustment is made for each pixel of the detector. This process corrects the spectral response at each wavelength so that the slave instruments produce the same spectra as the master instrument. It is important to use as broad a range of absorbances in the samples so that a good slope and bias estimate can be calculated. These Slope and Bias (S'||'&'||'B) factors are then downloaded into the slave instruments. Calibrations developed on the master instrument can then be downloaded onto the slave instruments and perform similarly to the master instrument. The data shown in this paper illustrates the process of calculating these S'||'&'||'B factors and the transfer of calibrations for wheat, barley and sorghum between several instruments.

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Mutilateralism vs. Bilateralism in Chinese Resource Diplomacy : Comparative Analysis on Chinese Foreign Policy toward Central Asia and Africa (중국 자원외교의 다자주의와 양자주의: 중앙아시아 및 아프리카에 대한 중국 자원외교 비교)

  • Kim, Ki-jung;Chun, Ja-hyun
    • Journal of International Area Studies (JIAS)
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    • v.13 no.1
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    • pp.107-133
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    • 2009
  • International relations in the 21st century is featured by boundless competition among nations to secure sufficient energy resources for achieving economic development. Resource diplomacy, therefore, is competitive in its nature, which is derived from the fact that resources of the globe are limited. Chinese recent economic growth has been possible mainly due to its success in resource diplomacy. The Chinese resource diplomacy has shown two different patterns according to target regions. On the one hand, China has pursued multilateral approach to Central Asia region, by which China aimed to secure energy with joint exploration method in the region. Chinese resource diplomacy toward Africa, on the other hand, has been based on bilateral approach combined with unit-centered economic aid to African nations. This difference in Chinese foreign policy pattern seems to be derived from three factors: namely, Chinese strategic considerations on geopolitical condition, regional security sensitivity, and legacies of Chinese long-time non-alignment diplomacy since the 1950s. Whether China is able to maintain the current pattern of resource diplomacy will be depended on how wisely China pursue its relations with two other global powers: Russia in the Central Asia and the U.S. in Africa. In this regard, the Chinese resource diplomacy is expected to work as determining factor of shaping a pattern of tri-lateral strategic relations among the U.S., Russia, and China. Chinese resource diplomacy thus will determine the future direction of the global politics in terms of strategic arrangement.

Examining the Object of Daesoonjinrihoe (대순진리회 목적(目的)에 관한 연구)

  • Yu, Byeong-mu
    • Journal of the Daesoon Academy of Sciences
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    • v.26
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    • pp.183-214
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    • 2016
  • The purpose of this study is to promote the understanding of the objective of Daesoon Thought through 'examining the Objectives of Daesoonjinrihoe.' The objective of Daesoon thought was set when Kang Jeungsan Sangje descended to human world, upon the plea of divine sages, buddhas, and bodhisattvas, and determined to save the world and relieve people far and wide. For building an Earthly Paradise with Daesoon thought, Sangje carried out the great work of Reordering of the Universe for nine years and passed into heaven. Cho Jeongsan Doju, who received the heavenly religious orthodox through divine revelation from Sangje, served the late will of Sangje and established Main Tenets, Creeds, and objective of Daesoon thought. The objective set by Doju was continued to Park Wudang Dojeon and formed the Objectives of Daesoonjinrihoe. The Objectives of Daesoonjinrihoe consist of three sets of objectives in sequential order, which are related in organic, mutual beneficient, and integrative with one another. The Objectives starts from individual cultivation and expand into worldly reformation: 1)one's Spiritual transformation through Guarding against self-deception 2)Renewal of human beings through Realizing Earthly Immortality 3)Opening of new world through Building of Earthly Paradise. Also, in these Objectives of Daesoonjinrihoe, Three Realms of Heaven, Earth, and Humanity keep close relations to enter into new world. These Objectives are based on the 'thought of mutual beneficence' and are realized with Daesoon thought, which is the Dao of 'Crowned King' that goes beyond the previous thoughts of Confucianism, Buddhism and Taoism. Objectives of Daesoonjinrihoe have following details in each objective as following; (1) 'Guarding against self-deception-Spiritual Transformation' is for human nature. In Daesoon thought, human has the potential to restore conscience, which is true nature, and return to undefiled essence by 'guarding against self-deception'. In other words, it views that one can reach into the stage of spiritual alignment with the Dao once he recovers conscience through cultivation of Daesoonjinrihoe based on the idea of 'Guarding against self-deception'. (2) 'Realizing Earthly Immortality-Renewal of human beings' is for ideal human character. 'Realizing Earthly Immortality' in Daesoon Thought indicates the advent of ideal human beings called 'Dotong gunja'(virtuous beings who are aligned with the Dao). They are leaders who integrate politics and religion in helping people to renew themselves and make the world into Earthly Paradise. (3) 'Building an Earthly Paradise-Opening of new world' is for ideal society. Daesoon thought aims to making a peaceful world by creating an ideal society of heaven on this earth. Park Wudang Dojeon, who led Daesoonjinrihoe, emphasized the importance of Three Basic Activities: Spreading of Sangje's virtue, Edification, and Cultivation: and Three Major Activities: Aid and charity, Social welfare, and Education. Dojeon said "One should make effort to reach the utmost goodness through enlightening their bright virtue and cultivating their talent virtue." In this words, he made clear that the Objectives of Daesoonjinrihoe can be realized when people enlighten their bright virtue and cultivate themselves with their talent virtue. In conclusion, the Objectives of Daesoon thought were clarified of their meanings by Sangje, established into laws by Doju, and actualized by Dojoen in various activities of Daesoonjinrihoe.

A Case Study of Hyundai Motors: Live Brilliant Campaign for Modern Premium Brand

  • Choi, Myounghwa;Lee, Yoonseo;Koo, Kay Ryung;Lee, Janghyuk
    • Asia Marketing Journal
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    • v.16 no.4
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    • pp.75-87
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    • 2015
  • As more companies become interested in global markets, it has become crucial for firms to create globalized brands whose positioning, advertising strategy, personality, looks, and feel are consistent across nations. The purpose of this study is to investigate the global branding strategy of the Hyundai Motor Company (hereafter HMC) in order to show how the company processes its branding strategy. HMC, one of the leading global companies in the automobile industry, set up its brand identity as "Modern premium", in alignment with their new slogan "New Thinking New Possibilities", in 2011. The aim of the "Modern premium" concept was to provide consumers with new experiences and values beyond their expectations. HMC wanted their consumers to think of their cars as not only a medium of transportation but as a life space, where they can share experiences alongside HMC. In an effort to conduct consumer research in 5 different nations, HMC selected "brilliant" as a key communication concept. The word "brilliant" expresses the functional, experiential, and emotional dimensions of HMC. HMC furthermore chose "live brilliant" as a key campaign message in order to reinforce their communication concept. After this decision, the "live brilliant" campaign was exhibited through major broadcast channels around the world. The campaign was the company's first worldwide brand campaign, where a single message was applied to all major markets, with the goal of building up a consistent image as a global brand. This global branding strategy is worth examining due to its significant contribution to growth generation in the global market. Overall, the 'live brilliant' global brand campaign not only improved HMC's reputation image-wise, with the 'Modern Premium' conceptualization of the brand as 'simple', 'creative' and 'caring', but also improved the consumer's familiarity, preference and purchase intention of HMC. In fact, the "live brilliant" campaign was a successful campaign which increased HMC's brand value. Notably, HMC's brand value increased continuously and reached 9 billion US dollars in 2013, leading it to reach 43rd place in the Global Brand Rankings according to the brand consulting group Interbrand. Its brand value largely surpassed that of Nissan (65th) and Chevrolet (89th) in 2013. While it is true that the global branding strategy of HMC involved higher risks, it was highly successful according to cross-nation consumer research. Therefore, this paper concludes that the global branding strategy of HMC made a positive impact on its performance. We further suggest HMC to combine its successful marketing with social media such as Facebook, Twitter, and Instagram and embrace digital media by extending its brand communication horizon to the mobile internet

Motion Vector Based Overlay Metrology Algorithm for Wafer Alignment (웨이퍼 정렬을 위한 움직임 벡터 기반의 오버레이 계측 알고리즘 )

  • Lee Hyun Chul;Woo Ho Sung
    • KIPS Transactions on Software and Data Engineering
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    • v.12 no.3
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    • pp.141-148
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    • 2023
  • Accurate overlay metrology is essential to achieve high yields of semiconductor products. Overlay metrology performance is greatly affected by overlay target design and measurement method. Therefore, in order to improve the performance of the overlay target, measurement methods applicable to various targets are required. In this study, we propose a new algorithm that can measure image-based overlay. The proposed measurement algorithm can estimate the sub-pixel position by using a motion vector. The motion vector may estimate the position of the sub-pixel unit by applying a quadratic equation model through polynomial expansion using pixels in the selected region. The measurement method using the motion vector can calculate the stacking error in all directions at once, unlike the existing correlation coefficient-based measurement method that calculates the stacking error on the X-axis and the Y-axis, respectively. Therefore, more accurate overlay measurement is possible by reflecting the relationship between the X-axis and the Y-axis. However, since the amount of computation is increased compared to the existing correlation coefficient-based algorithm, more computation time may be required. The purpose of this study is not to present an algorithm improved over the existing method, but to suggest a direction for a new measurement method. Through the experimental results, it was confirmed that measurement results similar to those of the existing method could be obtained.