• Journal of Korean Society on Water Environment
• /
• v.33 no.4
• /
• pp.441-448
• /
• 2017

Microcystis aeruginosa (M. aeruginosa) is a cyanobacterium species that can form harmful algal blooms in freshwater bodies worldwide. The use of Artemisia asiatica extracts to control M. aeruginosa inhibition will be environmentally friendly and promising. Artemisia asiatica extracts removed successfully upto 88% of M. aeruginosa pH 8 at $25^{\circ}C$ of temperature. These results was indicated that the amount of 2.24 g/L Artemisia asiatica extracts was removed 1g dryweight/L of M. aeruginosa. The kinetic data showed substrate inhibition kinetics and maximum growth rate was obtained when the M. aeruginosa was grown in medium containing 2.5 g/L of initial concentration of Artemisia asiatica extracts. In the various growth control models, Luong model showed the highest correlation coefficient of 0.9916. Therefore, the Luong model was the most suitable control model for the growth control of M. aruginosa using Artemisia asiatica extracts. In conclusion, the growth control of M. aruginosa using Artemisia asiatica extracts can be applied in the field without controlling the temperature and pH of rivers and streams, and it is possible to control the growth of M. aruginosa efficiently in a short time. The natural extract, Artemisia asiatica extracts, can be a promising inhibition due to its high efficiency and low dose requirements.

• Korean Journal of Environmental Biology
• /
• v.19 no.2
• /
• pp.129-135
• /
• 2001

The effects of CellCaSi and bioflocculant on the control of algal bloom were investigated in enclosures in a small eutrophic pond. The bioflocculant produced by a bacterial strain S-2 was finally selected to remove Microcystis aeruginosa which was a dominant species of algal bloom in the pond. Enclosure experiment showed that phosphorus concentration decreased dramatically from $131\mu{g}\ell^{-1}$ (Control) to $1-14\mu{g}\ell^{-1}$ in three CellCaSi-enriched enclosures. Chlorophyll $-\alpha$ concentration also decreased from $215\mu{g}\ell^{-1}$ (Control) to $59\mu{g}\ell^{-1}$ by the addition of CellCaSi $(1g\ell^{-1}$, bioflocculant $(2ml\ell^{-1}$, calcium chloride $(1g\ell^{-1}$ and ferric chloride $(2mg\;Fe\ell^{-1})$ in Enclosure 4. From the results of the mouse acute toxicity test of the S-2 bioflocculant and the goldfish survival test in enclosures, it seems that both the S-2 bioflocculant and the CellCaSi do not show any severe toxicity in water system. Consequently, it was concluded that the bioflocculant and the CellCaSi could be used to control algal bloom in eutrophic waters by removing phosphorus and chlorophyll$-\alpha$.

• Journal of Korean Society on Water Environment
• /
• v.26 no.3
• /
• pp.420-431
• /
• 2010

Simple material budget models were developed to predict the dry season water quality for a river-type reservoir in Paldang, Republic of Korea. Of specific interest were the total phosphorus (TP), chlorophyll ${\alpha}$ (Chl. ${\alpha}$), 5-day biochemical oxygen demand (BOD), and chemical oxygen demand (COD). The models fit quite well with field data collected for 20 years and have enabled the identification of the origins of organic materials in the reservoir. The critical hydraulic load that determines the usability of phosphorus for algal production appeared to be about $1.5m\;d^{-1}$. When a hydraulic load was smaller than the critical value, the concentrations of $Chl.{\alpha}$, COD, and BOD in the reservoir water became sensitive to internal algal reactions such as growth, degradation, and settling. In spite of the recent intensive efforts for organic pollutant removal from major point sources by central and local governments, the water quality in the reservoir had not been improved. Instead, the concentration of COD increased. The model analysis indicated that this finding could be attributed to the continuing increase of the algal production in the reservoir and the allochthonous load from non-point sources. In particular, the concentrations of COD and BOD of algal origin during 2000~2007, each of which is comprised of approximately one half of the total, were approximately 2.5 times higher than those observed during 1988~1994 and approximately 1.3 times higher than those between 1995~1999. The results of this study suggested that it is necessary to reduce the algal bloom so as to improve the water quality of the reservoir.

• Journal of Korean Society of Water and Wastewater
• /
• v.32 no.2
• /
• pp.97-106
• /
• 2018

In this study, the effect of chemically enhanced backwash(CEB) coping with algal(Heterosigma Akashiwo) inflow was evaluated in the seawater desalination pretreatment process using ceramic membrane. In order to confirm the possibility of long-term filtration operation, the recovery rate of transmembrane pressure(TMP) due to the CEB using NaOCl was examined. When the membrane flow rate was 83.3 LMH, the TMP was maintained within 200 kPa for 84 hours in seawater influent. As the algal counts of 30,000 cell/mL were injected into the influent of seawater, however, the TMP rapidly increased and exceed maximum value. Membrane fouling caused by the algae was very poorly recovered by usual physical backwash. The CEB was performed for 30 min(3 min circulation / 27 min immersion) with 300 mg/L of NaOCl. As a result of the CEB application, it was possible to maintain a stable operating of filtration during 10 days and the average recovery rate of TMP by the CEB was 98.1%. It has been confirmed that the CEB using NaOCl is very effective in removal of membrane fouling by algae, resulted in stable membrane filtration for the long-term operation.

• Korean Journal of Ecology and Environment
• /
• v.49 no.3
• /
• pp.176-186
• /
• 2016

For several decades, lactic acid bacterium (Lactobacillus graminis: LAB) has been generally recognized as safe. To develop the pan-environmental bio-control agent, algicidal activity of the live LAB cell and its culture filtrate (CF) was examined against Microcystis aeruginosa. LAB cells perfectly lysed M. aeruginosa within 3 days, while the CF had a less effect than the live cells, approximately 78% inhibition of algal growth during a same culture period. The concentration of microcystin in alone culture of M. aeruginosa was $7.1{\mu}gL^{-1}$, but gradually increased and leach $158.5{\mu}gL^{-1}$ on 10 days. However, LAB cells clearly decreased the microcystin by $10.3{\mu}gL^{-1}$ in the same period, approximately 93.5%. CF of LAB showed a strong algicidal activity over 75% between pH 2-7, 91.3% by the treatment of proteinase K, 87.8% by below 3 kDa in particle size, and 75.3% by heat treatment, respectively. Of five solvents, fractions of CF passed through solvents diethyl ether and ethyl acetate showed an obvious algicidal activity in the algal-lawn test. Among 5 fractions purified by silica-gel TLC plate, two spots showed a most strong removal activity on M. aeruginosa. Another analysis of GC indicate that CF contained six representative fatty acids. Even though most of these substance have been known as an anti-algal substance against M. aeruginosa, oleic acid is the most effective. These results suggested that the culture filtrate or specific substances, like a fatty acids, in comparison with live L. graminis can be a successful and eco-friendly agent to control Microcystis bloom.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.20 no.3
• /
• pp.83-88
• /
• 2012

The algal bloom, resulting from eutrophication, has caused serious water quality problems in river and lake. Therefore, it has to be removed by any means including physicochemical or biological treatment for preserving water quality. This study was conducted to investigate the microalgae removal and energy production using combined electro-flotation and anaerobic hydrogen fermentation processes. The result showed that algae removal efficiency based on chlorophyll a removal increased with the current. At a current of 0.6A, the maximum microalgae removal efficiency of 95.9% was achieved. The treatability of anaerobic hydrogen fermentation was investigated to recover energy from microalgae removed by electro-flotation. The ultimate hydrogen yields of algae before and after ultrasonic pretreatment were 17.3 and 61.1 ml $H_2/g$ dcw(dry cell weight), respectively. The ultrasonic pretreatment of algae led to 3.4-fold higher $H_2$ production due to the increase of hydrolysis rate.

• Journal of Microbiology and Biotechnology
• /
• v.10 no.4
• /
• pp.455-461
• /
• 2000

Abstract Spirulina platensis was grown in SWlUe waste to reduce inorganic compowlds and simultaneously produce feed resources. Spirulina platensis prefers nitrogenous compounds in Ibe order: $NH_4^{+}-N>NO_3^{-}-N>simple-N$ such as urea and simple amino acids. It even consumes $NH_4^{+}-N$ first when urea or nitrate are present. Therefore, the content of residual $NH_4^{+}-N$ in Spimlina platensis cultures can be determined by the relative extent of the following processes: (i) algal uptake and assimilation; (ii) ammonia stripping; and (iii) decomposition of urea to NH;-N by urease-positive bacteria. The removal rates of total nitrogen ffild total phosphorus were estimated as an indicator of the treatment effIciency. It was found that Spirulina platensis was able to reduce 70-93% of $P_4^{3-}-P$, 67-93% of inorganic nitrogen, 80-90% of COD, and 37-56% of organic nitrogen in various concentrations of swine waste over 12 days of batch cultivation. The removal of inorganic compounds from swine waste was mainly used for cell growth, however, the organic nitrogen removal was not related to cell growlb. A maximum cell density of 1.52 dry-g/l was maintained with a dilution rate of 0.2l/day in continuous cultivation by adding 30% swine waste. The nitrogen and phosphorus removal rates were correlated to the dilution rates. Based on the amino acid profile, the quality of the proteins in the Spirulina platensis grown in the waste was the same as that in a clean culture.ulture.

• Journal of Korean Society of Water and Wastewater
• /
• v.31 no.6
• /
• pp.577-586
• /
• 2017

One of the major sources causing eutrophication and algal blooms of lakes or streams is phosphorus which comes from point and nonpoint pollution sources. HAP (hydroxyapatite) crystallization using granular alkaline materials can achieve the decrease of phosphorus load from wastewater treatment plants and nonpoint pollution control facilities. In order to induce HAP crystal formation, continuous supply of calcium and hydroxyl ions is required. In this research, considering HAP crystallization, several types of lime-based granular alkaline materials were prepared, and the elution characteristics of calcium and hydroxyl ions of each were analyzed. Also, column tests were performed to verify phosphorus removal efficiencies of granular alkaline materials. Material_1 (gypsum+cement mixed material) achieved the highest pH values in the column tests consistently, also, Material_2 (gypsum+slag mixed material) and Material_3 (calcined limestone material) achieved over pH 9.0 for 240 hours (10 days) and proved the efficiencies of long-term ion supplier for HAP crystallization. In the column tests using Material_3, considerable pH increase and phosphorus removal were carried out according to each linear velocity and filtration depth. T-P removal efficiencies were 87.0, 84.0, 68.0% and those of PO4-P 100.0, 97.0, 80.0% for linear velocity of 1.0, 2.5, 5.0 m/hr respectively. Based on the column test results, the applicability of phosphorus removal processes for small-scale wastewater treatment plants and nonpoint pollution control facilities was found out.

• Journal of Wetlands Research
• /
• v.19 no.4
• /
• pp.508-514
• /
• 2017

Water-bloom and red tide due to eutrophication have been overgrown and have caused various environmental problems. Recently, however, research on bid-diesel that can utilize algae as an energy source has been actively carried out. In particular, many studies variously have been conducted to utilize algal photosynthesis oxygen as a supply method for reducing the energy by an air blower in MWTP. In this study, a lab scale algae-nitrification reactor was operated to replace the oxygen required for nitrogen removal and the operation period was largely divided into three sections. In the first section, ammonia nitrogen removal efficiency was 24 ~ 38% according to the MLSS (Mixed Liquer Suspended Solid) concentration. In the second section, ammonia nitrogen removal efficiency was 38 ~ 50% according to the micro-algae concentration and in the last section ammonia nitrogen removal efficiency was 61 ~ 80% according to HRT (Hydraulic Retention Time). As a result, as the MLSS decreased and algae biomass increased, the ammonia nitrogen removal efficiency tended to increase, but the effect of Algae biomass was greater than that of MLSS.

• Journal of Environmental Science International
• /
• v.11 no.11
• /
• pp.1157-1163
• /
• 2002

The effective removal of microcystins by chlorination was investigated on a laboratory scale. With an initial chl.a concentration of more than 1,000 $\mu\textrm{g}$/ℓ, the required chlorine dose for the effective removal of microcystins from the raw water was more than 8.0 mg/ℓ. Whereas, a chlorine dose of 3.0 mg/ℓcould effectively remove microcystins from raw water containing a chl.a concentration of less than 1,000 $\mu\textrm{g}$/ℓ. The microcystin removal was more effective below pH 8.0, plus the optimum pH range was unrelated to the concentration of toxic algal material. Although chlorination is one of the most effective methods for reducing the toxin from blue-green algae, it causes cell lysis and toxin release. However, it was demonstrated that the released cell lysates and toxins could be effectively removed by a higher dose of the oxidant. The highest removal efficiency of dissolved microcystins(initial concentration: 280 $\mu\textrm{g}$ L$\^$-1/) was with a chlorine dose of 5.0 mg/ℓ.