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http://dx.doi.org/10.11614/KSL.2016.49.3.176

Inhibition of Growth and Microcystin Toxicity, and Characterization of Algicidal Substances from Lactobacillus graminis against Microcystis aeruginosa  

Joo, Jae-Hyoung (Department of Life Science, Hanyang University)
Park, Bum Soo (Department of Life Science, Hanyang University)
Lee, Eun-Seon (Department of Life Science, Hanyang University)
Kang, Yoon-Ho (Department of Life Science, Hanyang University)
Han, Myung-Soo (Department of Life Science, Hanyang University)
Publication Information
Korean Journal of Ecology and Environment / v.49, no.3, 2016 , pp. 176-186 More about this Journal
Abstract
For several decades, lactic acid bacterium (Lactobacillus graminis: LAB) has been generally recognized as safe. To develop the pan-environmental bio-control agent, algicidal activity of the live LAB cell and its culture filtrate (CF) was examined against Microcystis aeruginosa. LAB cells perfectly lysed M. aeruginosa within 3 days, while the CF had a less effect than the live cells, approximately 78% inhibition of algal growth during a same culture period. The concentration of microcystin in alone culture of M. aeruginosa was $7.1{\mu}gL^{-1}$, but gradually increased and leach $158.5{\mu}gL^{-1}$ on 10 days. However, LAB cells clearly decreased the microcystin by $10.3{\mu}gL^{-1}$ in the same period, approximately 93.5%. CF of LAB showed a strong algicidal activity over 75% between pH 2-7, 91.3% by the treatment of proteinase K, 87.8% by below 3 kDa in particle size, and 75.3% by heat treatment, respectively. Of five solvents, fractions of CF passed through solvents diethyl ether and ethyl acetate showed an obvious algicidal activity in the algal-lawn test. Among 5 fractions purified by silica-gel TLC plate, two spots showed a most strong removal activity on M. aeruginosa. Another analysis of GC indicate that CF contained six representative fatty acids. Even though most of these substance have been known as an anti-algal substance against M. aeruginosa, oleic acid is the most effective. These results suggested that the culture filtrate or specific substances, like a fatty acids, in comparison with live L. graminis can be a successful and eco-friendly agent to control Microcystis bloom.
Keywords
lactic acid bacteria; Lactobacillus; algicidal bacteria; Microcystis aeruginosa; eco-friendly mitigation;
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Times Cited By KSCI : 2  (Citation Analysis)
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