• Title/Summary/Keyword: Alcohol Formation

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Gentisyl Alcohol, an Antioxidant from Microbial Metabolite, Induces Angiogenesis In Vitro

  • Kim Hye-Jin;Kim Jin-Hee;Lee Choong-Hwan;Kwon Ho-Jeong
    • Journal of Microbiology and Biotechnology
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    • v.16 no.3
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    • pp.475-479
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    • 2006
  • Gentisyl alcohol isolated from Penicillium sp. has an antioxidative activity, protecting cells from oxidative stresses. From our in vitro angiogenesis assays with bovine aortic endothelial cells (BAECs), gentisyl alcohol was newly identified as a pro-angiogenic small molecule that induces new blood vessel formation of the cells. Gentisyl alcohol stimulated the proliferation of BAECs in a dose-dependent manner. Moreover, it induced in vitro angiogenesis of BAECs such as invasiveness, migration, and tube formation of the endothelial cells. Effects of gentisyl alcohol on invasion and tube formation were also dose-dependent. These results demonstrate that gentisyl alcohol could affect the angiogenic phenotypes of endothelial cells and be developed as a new small molecule with pro-angiogenic activity.

Enzymatic Formation of Guaiacylglycerol 8-O-4'-(Coniferyl Alcohol) Ether from Coniferyl Alcohol with Enzyme Preparations of Eucommia ulmoides

  • Alam, Md. Shameul;Katayama, Takeshi;Suzuki, Toshisada;Sultana, Deeder;Sultana, Saima;Hossain, Md. Daud
    • Journal of Crop Science and Biotechnology
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    • v.11 no.1
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    • pp.45-50
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    • 2008
  • Lignans and neolignans are optically active plant secondary metabolites. Research on biosynthesis of lignans has already been advanced especially for the formation of (+) pinoresinol but information on the biosynthesis of 8-O-4'- neolignans is still limited. Moreover, the chemical structure(position of substituents on aromatic rings) and stereochemistry of 8-O-4' neolignans is not clear. Katayama and Kado discovered that incubation of cell-free extracts from E. ulmoides with coniferyl alcohol in the presence of hydrogen peroxide gave (+)-erythro- and (-)-threo- guaiacylglycerol 8-O-4'-(coniferyl alcohol) ether (GGCE)(diastereomeric ratio, 3:2) which is the first report on enzymatic formation of optically active -8-O-4' neolignans from an achiral monolignol. In this aspect, enzymatic formation of guaiacyl 8-O-4' neolignan is noteworthy to clarify its stereochemistry from incubation of coniferyl alcohol with enzyme prepared from Eucommia ulmoides. In this experiment, soluble and insoluble enzymes prepared from E. ulmoides were incubated with 30 mM coniferyl alcohol(CA) for 60 min. The enzyme catalyzed GGCE, dehydrodiconiferyl alcohol(DHCA), and pinoresinol identified by reversed phase HPLC. Consequently, diastereomeric compositions of GGCE were determined as erythro and threo isomer. Enantiomeric composition was determined by the chiral column HPLC. Both enzyme preparations enantioselectively formed (-)-erythro, (+)-erythro and (+)-threo, (-)-threo-GGCEs respectively.

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Effect of Liuweidihuang-tang on Alcohol-induced Decrease in New Cell Formation in Rat Dentate Gyrus

  • Bahn Goon Ho;Yoon Doh Joon;Park Jin Kyung;Lee Taeck Hyun;Jang Mi Hyoon;Shin Min Chul;Kim Chang Ju;Paik Eun Kyung;Park Jae Hyung;Cho Son Hae;Lee Choong Yool
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.16 no.5
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    • pp.1055-1059
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    • 2002
  • Liuweidihuang-tang has been traditionally used for the treatment of delayed mental and physical development in children, complications of diabetes. and glomerulonephritis. In the present study, the effect of Liuweidihuang-tang on cell proliferation in the dentate gyrus of alcohol-intoxicated Sprague-Dawley rats was investigated via 5-bromo-2' -deoxyuridine (BrdU) immunohistochemistry. Alcohol administration was shown to decrease the number of BrdU-positive cells in the dentate gyrus, while Liuweidihuang-tang treatment increased new cell formation in the dentate gyrus under normal conditions and alcohol intoxication. It was thus demonstrated that aqueous extract of Liuweidihuang-tang exerts a protective effect against alcohol-induced decrease in new cell formation.

The Effect of Iron Catalysts on the Formation of Alcohol and Ketone in the Biomimetic Oxidation of Cyclohexane

  • Kim Seong-Bo;Lee Kyu-Wan;Kim, Yong-Joon;Hong Seog-In
    • Bulletin of the Korean Chemical Society
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    • v.15 no.6
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    • pp.424-427
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    • 1994
  • Effects of iron compounds in known biomimetic oxidation systems (Gif IV and GoAgg II) have been studied on activity and ketone/alcohol selectivity of cyclohexane oxidation. Both ketone/alcohol ratio and cyclohexane conversion were affected by counter-ion Z of iron compounds Z-Fe. When Z has a more electron withdrawing property, the reactivity is increased and the formation of ketone is favored. From these experimental results, a new mechanism is proposed for the biomimetic oxidation system.

Isolation and Identification of Adventitious Root Formation Inducing Substances from Cortex of cinnamomum cassia J.Presl (육계(Cortex of Cinnamomum cassia J.Presl) 추출물로부터 부정근 형성 유도물질 분리 및 동정)

  • Joo Ho Yeo;Jeong Kyu Baek;Jee Sung Park;Kun Woo Kim
    • Korean Journal of Plant Resources
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    • v.37 no.1
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    • pp.11-21
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    • 2024
  • In this study, as a result of exploring the physiological activity of plants useful for agriculture on various plant resources, it was possible to confirm an activity similar to auxin that promotes plant rooting in methanol extract of Cinnamon Bark (cortex of Cinnamomum cassia J.Presl). After separating the active body by applying column chromatography and HPLC to the CHCl3 active fraction obtained by solvent extraction for each polarity from the methanol extract of cinnamon bark, cinnamyl alcohol was identified through GC/MS analysis. By bioassay using cinnamyl alcohol standard and the active fraction separated and purified from the methanol extract of cinnamon bark, the rooting rate of mung bean seedlings of the cinnamyl alcohol standard was 290% compared with the untreated control at 134.2 ㎍/mL concentration, and the adventitious root formation activity similar to the rooting rate (268.6%; 100 ㎍/mL) of the active fraction was shown. In conclusion, it is believed that cinnamyl alcohol contained in methanol extract of Cinnamon Bark is the main compound that induces adventitious root formation in mung bean.

Effect of ethyl alcohol aging on the apatite formation of a low-modulus Ti-7.5Mo alloy treated with aqueous NaOH

  • Ho, Wen-Fu;Tsou, Hsi-Kai;Wu, Shih-Ching;Hsu, Shih-Kuang;Chuang, Shao-Hsuan;Hsu, Hsueh-Chuan
    • Biomaterials and Biomechanics in Bioengineering
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    • v.1 no.1
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    • pp.51-62
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    • 2014
  • The purpose of this experiment was to evaluate the apatite-formation abilities of low-modulus Ti-7.5Mo substrates treated with NaOH aqueous solutions and subsequent ethyl alcohol aging before soaking them in simulated body fluid. Specimens of Ti-7.5Mo were initially treated with 5 M NaOH at $60^{\circ}C$ for 24 h, resulting in the formation of a porous network structure composed of sodium hydrogen titanate. Afterwards, the specimens were aged in ethyl alcohol at $60^{\circ}C$ for 5 or 10 min, and subsequently immersed in simulated body fluid at $37^{\circ}C$ for 3, 7 and 14 days. Ethyl alcohol aging significantly increased the apatite-forming abilities of Ti-7.5Mo. The amount of apatite deposited on the Ti-7.5Mo after NaOH treatment and subsequent ethyl alcohol aging was much greater, especially after the Ti-7.5Mo specimens were aged for 5 min. Due to its excellent combination of bioactivity, low elastic modulus and low processing costs, the Ti-7.5Mo treated with NaOH aqueous solutions and subsequently aged in ethyl alcohol has promising heavy load-bearing applications.

Inhibition of in vitro Nitrosation by Capsaicin and Its Metabolites (Capsaicin 및 그 대사산물에 의한 in vitro에서의 니트로소 화합물 생성 저해효과)

  • 유리나;박정순;박건영
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.5
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    • pp.1015-1018
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    • 1998
  • Capsaicin(8-methyl-N-vanillyl-6-nonenamide: CAP) known well as a major compound of not taste in hot pepper, was investigated for the inhibition effect on in vitro nitrosation. CAP(100$\mu$mol) inhibited the formation of N-nitrosoproline(NPRO) and N-nitrosothioproline(NTPRO) by 56% and 26%, respectively. Vanillyl alcohol inhibited the nitrosation of proline by a concentration-dependent manner, and vanillic acid and vanillin were less effective in blocking the nitrosation of proline compared to CAP and anillyl alcohol. The inhibitory effect of NPRO formation by CAP was evaluated to similar with alpha-tocopherol, and vanillyl alcohol was more effective than alpha-tocopherol in blocking the nitrosation of proline. Our results suggested that CAP and its metabolites such as vanillyl alcohol could inhibit endogenous nitrosation in hydrophobic biological environment.

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Influence of the Syndiotacticity of Poly(vinyl alcohol) block on the formation of micelles for Poly(vinyl alcohol-b-styrene) (Poly(vinyl alcohol) block의 신디오탁틱이 Poly(vinyl alcohol-b-styrene)의 micelle형성에 미치는 영향)

  • 이광화;조창기
    • Proceedings of the Korean Fiber Society Conference
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    • 2003.04a
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    • pp.291-292
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    • 2003
  • Amphiphilic block copolymer는 self-assembly특성을 가지고 있기 때문에 선택된 용매 속에서 nanoscale-domain 즉 micelle을 형성할 수 있다. 이러한 특성은 기타 불용성 물질을 가용화하고 colloidal particle을 안정화시키거나 micro-emulsion을 형성할 수 있어 pharmaceutics, drug delivery system등 영역, 그리고 emulsion stabilizer, thickener, dispersion agent등으로 사용될 수 있다. (중략)

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AN EXPERIMENTAL STUDY ON THE EFFECT OF ALCOHOL INJECTION IN RAT ORAL MUCOSA (알콜(Alcohol)주사가 구강조직에 미치는 영향에 관한 실험적 연구)

  • Min, Byong-Il
    • The Journal of the Korean dental association
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    • v.15 no.12
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    • pp.957-962
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    • 1977
  • The author has observed the tissue reaction of the absolute alcohol infection of rat oral mucosa. 0.5ml absolute alcohol was injected subcutaneously on the mucobuccal fold of rat. And the rats were sacrifieced at intervals of one day, 3rd, 1 week, 2 week and 4 week after alcohol injection. The microscopic tissue sections were made and stained with hematoxylin and eosin. The results were are as follows; 1. Degeneration and shrinkage of fibroblasts and coagulative necrosis were observed one day to and three day after alcohol injection. 2. Although coagulative necrosis and tissue degeneration occurred, the inflammatory infiltration was not prominent especially there were scarcely any polymorphonuclear leukocytes in that field. 3. Granulation tissue with moderate small round cell infiltration were replaced the necrotic area at one week after injection and the fibroblast proliferate into the granulation tissue at two week group. 4. At four week after injection, the damaged area recovered by fibroblastic proliferation and collage formation, but there were

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